Click for Details
Figure G1.1  Cutaway View of Galvo-Galvo Scan Head
The scan lens being used with the scan head needs to be positioned such that its entrance pupil is at the midpoint of the 4.33 mm green line.

• Optical Scan Range of ±15° (±7.5° Mechanical Scan Range) for Beams Up to Ø4 mm
• Driven by User-Supplied DC, Sine, Triangle, or Sawtooth Wave Signal
• BNC Connectors and NI 68-Pin Connector for Position Control and Readout
• Protected Silver Coating for High Reflectance in Visible and NIR
• Scan Head is SM05, SM1, 30 mm Cage, and Post Mountable

Our LSKGG4 Galvo-Galvo Scanner utilizes two galvo scan mirrors that deflect an incident laser beam in X and Y. Identical to the galvo-galvo scanner used in our Bergamo III multiphoton microscopes, four-channel Cerna-based confocal systems, and Veneto inverted microscopy platform, each mirror has a mechanical scan range of ±7.5°, corresponding to an optical scan range of ±15°. This range is large enough to explore the entire diffraction-limited FN20 field of view of Thorlabs' SL50 scan lenses for laser scanning microscopy.

A National Instruments (NI) 68-pin connector is available to directly control and monitor the scanner via an NI card or breakout box (not included). The beam can also be steered by supplying DC, triangle, sawtooth or sine wave drive signals through two BNC connectors. Two additional BNC connectors provide X and Y position feedback signals. This scanner is also compatible with Vidrio's ScanImage software.

Each scan mirror has a protected silver coating that provides high reflectance in the visible and NIR. Click here for a typical reflectance curve at a 45° AOI. Since the coating is metallic, its performance varies minimally over the ±7.5° mechanical scan range of the mirror.

The scan head is connected to the controller using the included DB25 cable. If using the BNC input connectors to drive the galvo mirrors, a drive voltage of ±10 V at an impedance of 10 kΩ will be required for each axis. The BNC output connectors have a voltage range of ±3.75 V and an impedance of 1 kΩ.

Complete specifications and usage details are available at our full web presentation.

Mounting
The input face of the scan head has internal SM05 (0.535"-40) threads, internal SM1 (1.035"-40) threads, and four Ø6 mm bores spaced for our 30 mm cage system, while the output face has internal SM1 threads and four 4-40 taps for our 30 mm cage system. The scan lens needs to be positioned such that its entrance pupil is at the midpoint between the galvo mirrors; when being used at the input, the distance is 27.255 mm, while at the output, the distance is 29.045 mm. For the CLS-SL Scan Lens, an LCP02 (/M) Cage System Size Adapter can be used to convert the 30 mm cage system to a 60 mm cage system to mount and position this scan lens at the correct distance using a LCP8S Cage Plate. An SM1A70 adapter (sold below) with external SM1 and internal SM30 mounting threads will position our SL50-CLS2, SL50-2P2, or SL50-3P Scan Lens at the correct distance from the scanner output port.

The scan head has a mass of 0.56 kg, so it may be useful to support it directly using a Ø1/2" post or Ø1" post. Four 1/4"-20 (M6) tapped holes are located on the bottom.

Click to Enlarge
Figure G2.1  Cutaway View of Galvo-Resonant Scan Head
The scan lens being used with the scan head needs to be positioned such that its entrance pupil is at the midpoint of the 7.13 mm green line between the resonant mirror (X) and the galvo mirror (Y).

Item #a	LSK-GR08	LSK-GR12
Galvo Scan Range	±7.5° Mechanical (±15° Optical)
Resonant Scan Range	±5° Mechanical (±10° Optical)	±2.5° Mechanical (±5° Optical)
Beam Diameter	5 mm (Max)
Resonant Scanner Frequency	7910 ± 15 Hz	12 000 ± 15 Hz
Galvo BNC Input Bandwidth	0 - 500 Hz (Max)b

• Please see the full web presentation for a complete list of specifications.
• Bandwidth measured for a ±10 V sine wave. Results vary depending on the amplitude of the waveform.

• 8 kHz or 12 kHz Resonant Scanner Frequency for High-Speed XY Scans
• Control via BNC Connectors, Software, and NI 68-Pin Connector
• Protected Silver Coating for High Reflectance in Visible and NIR
• Scan Head is SM1, 30 mm Cage, and Post Mountable

Our Galvo-Resonant (GR) Scanners are available with 8 kHz or 12 kHz resonant scanner frequency. Each scanner contains one resonant scan mirror and one galvo scan mirror that deflect an incident laser beam in X and Y, respectively. The resonant frequency of the resonant scan mirror enables much higher-speed scans than a galvo-galvo system. The LSK-GR08(/M) 8 kHz and LSK-GR12(/M) 12 kHz GR scanners are used in our Bergamo III multiphoton microscopes, four-channel Cerna-based confocal systems, and Veneto inverted microscopy platform. In our Bergamo system, the 8 kHz GR scanner can achieve 30 fps at 512 x 512 pixels image resolution, while the 12 kHz GR scanner can achieve 45 fps at 512 x 512 pixels image resolution.

The beam can be steered via two BNC connectors for simple uni-directional scans. For fine tuning and bi-directional scanning functionality, we provide a GUI for Windows^® to adjust the scan range of the resonant scan mirror and the sync signal offset, as well as a National Instruments (NI) 68-pin connector to directly control and monitor the galvo scan mirror via an NI card or breakout box (not included). See the full web presentation for details. Each scanner is also compatible with Vidrio's ScanImage software.

Each scan mirror has a protected silver coating that provides high reflectance in the visible and NIR. Click here for a typical reflectance curve at a 45° AOI. Since the coating is metallic, the performance varies minimally over the scan range of the mirror. The scan head input port also features an NIR window that, when combined with a scan lens on the output port, greatly reduces the audible noise from the system.

Mounting
The input face of each scan head has four Ø6 mm bores spaced for our 30 mm cage system. By removing the NIR window installed on the input face using a SPW801 spanner wrench, internal SM05 (0.535"-40) and internal
SM1 (1.035"-40) threads become accessible. The output face is equipped with internal SM1 threads and four 4-40 taps for our 30 mm cage system.

The scan lens needs to be positioned such that its entrance pupil is at the midpoint between the galvo and resonant mirrors. The distance from the input face to the midpoint is 28.655 mm, while the distance from the output face to the midpoint is 28.965 mm. An SM1A70 adapter (sold below) with external SM1 and internal SM30 mounting threads will position our SL50-CLS2, SL50-2P2, or SL50-3P Scan Lens at the correct distance from the scanner output port.

The scan head has a mass of 0.76 kg, so it may be useful to support it directly using a Ø1" post. Four 1/4"-20 (M6) tapped holes are located on the bottom.

• Telecentric Multiphoton Tube Lenses, and Telecentric Scanning and Tube Lens Pairs
• AR Coated for Single-, Two-, or Three-Photon Excitation
  • TTL200MP: 400 - 1300 nm
  • TTL200MP2: Broadband Single-Layer MgF₂ Coating
  • SL50-CLS2: 450 - 1100 nm
  • SL50-2P2: 680 - 1300 nm
  • SL50-3P: 800 - 1800 nm
• Telecentric Design Provides Constant Spot Sizes Across the Field of View
• Diffraction-Limited Field of View Up to FN20 at Objective Plane, Depending Upon Wavelength
• Each Scan and Tube Lens is Individually Corrected for Aberrations
• Optic Designs Based on Our Bergamo III Multiphoton Microscopes, Four-Channel Cerna-Based Confocal Systems, and Veneto Inverted Microscopy Platform

Multiphoton Telecentric Tube Lens
Thorlabs' TTL200MP and TTL200MP2 infinity-corrected telecentric tube lenses are optimized for multiphoton imaging and other laser scanning applications. These tube lenses feature diffraction-limited axial color correction and resolution over the broadband wavelength range of 400 - 2000 nm, and are AR coated. The TTL200MP tube lens provides higher transmission at visible and NIR wavelengths, while the TTL200MP2 tube lens provides higher transmission at longer wavelengths approaching 2000 nm. Their effective focal length of 200 mm is the design focal length used by Thorlabs, Nikon, Leica, and Mitutoyo objectives. Click the Complete Tube Lens Specifications box bar for performance data.

Due to the large design wavelength range of these tube lenses, they can be paired with any of the scan lenses featured below.

Telecentric Tube and Scan Lens Pairs
These scan and tube lenses have been optimized for point-by-point (raster) laser scanning within one of three super broadband wavelength ranges: 450 - 1100 nm, 680 - 1300 nm, or 800 - 1800 nm. Optimized for single-, two-, and three-photon excitation, respectively, they support a diverse range of experimental needs, including confocal and multiphoton imaging, as well as photostimulation and uncaging. Depending upon the lens and the wavelengths used, field numbers up to 20 (i.e., an entrance pupil at the objective up to Ø20 mm) are supported, as shown by clicking on the Complete Lens Specifications boxes. For reference, if a 20X objective is used with a lens pair that supports a field number of 20, the field of view will be Ø1 mm.

Telecentric lens systems produce spot sizes in the sample plane that are nearly constant over the entire field of view, resulting in image resolution that varies minimally over the scanned area of the sample. Additional information on telecentricity is available from our telecentric lenses tutorial. Our scan and tube lenses are each individually corrected for aberrations, so it is also possible to use this tube lens with another aberration-corrected scan lens.

Mounting Ideas
Our scan lenses have external SM30 (M30 x 0.5) mounting threads on the end that should face the galvo scanning system. Please note that the scan lens needs to be positioned such that the scanning distance is at the midpoint between the galvo and resonant mirrors. Because of the 37.8 mm scanning distance, these lenses need to be placed in close proximity to the scanner. An SM1A70 adapter (sold below) with external SM1 and internal SM30 mounting threads will position the scan lens at the correct distance from the scanner output port when used with the scanners sold on this page.

Our tube lenses have external SM2 threads on both sides that can be used to mount the tube lens into a Ø2" lens tube or a 60 mm cage plate. If using the OPX2400(/M) Breadboard Top with Two-Position Slider, the tube lens can also be threaded directly into the optic slider.

These tube lenses are engraved with an infinity symbol (∞) next to an arrow to indicate which side of the lens should face the objective (infinity space).

Complete Tube Lens Specifications

Table G3.1  Specifications
Item #	TTL200MP	TTL200MP2
Design Wavelength Range	400 - 2000 nm
AR Coating Range	400 - 1300 nm	Broadband Single-Layer MgF2 Coating
Effective Focal Length	200 mm
Working Distancea	151.4 mm
Pupil Distanceb	228 mm (Telecentric)
Track Length	420 mm (Nominal)
Entrance Pupil Diameter	20 mm
f/#	10
Field Size (Diffraction Limited)	Ø22 mm
Clear Aperture	Ø36.8 mm
Lens Design	Apochromatic
Axial Color	Diffraction Limited
F-Theta Distortion	<0.2%
Threading	External SM2 Threads (Top and Bottom)
Housing Length	44.1 mm
Performance Data (Click for Graph)
Transmission
Axial Color
RMS Wavefront Error
MTF
Data	Excel Spreadsheet	Excel Spreadsheet
Zemax Black Box Files	Forward Backward	Forward Backward

• Measured from the housing edge to the intermediate image plane (see Figure G3.2).
• This is the optimal distance between the lens and the objective (see Figure G3.2).

Click To Enlarge
Figure G3.2  This schematic shows the TTL200MP Tube Lens in a laser scanning configuration with the working distance and pupil distance called out. The working distance corresponds to the distance from the edge of the housing below the engraving to the intermediate image plane. The pupil distance is defined as the distance between the edge of the tube lens housing above the engraving and the entrance pupil of the objective.

These lenses are engraved with an arrow next to an infinity symbol (∞) to indicate which side of the lens should face the objective (infinity space), as shown in Figure G3.2.

Complete Scan Lens Specifications

The complete specifications available for each scan lens are provided in Table G3.3, and definitions of the various parameters follow the table. Click the blue icons, , to view the plots. All plots below show theoretical data.

Table G3.3  Specifications
Scan Lens Item #		SL50-CLS2	SL50-2P2	SL50-3P
Optical Specifications
Wavelength Range		450 - 1100 nm	680 - 1300 nm	800 - 1800 nm
Effective Focal Length (EFL)		50 mm
Entrance Pupil Diametera		4 mm	5 mm
Parfocal Distance		77.3 mm
Lens Working Distance		26.4 mm
Scanning Distanceb		37.8 ± 4 mm
Optical Scan Anglec (Single-Axis Scan)	Maximum	±11.5°
	Diffraction Limited	±7.5° (at 486.1 nm) ±9° (at 587.6 nm) ±11.5° (656.6 - 1100 nm)	±7.2° (at 680 nm) ±8° (at 800 nm) ±11.5° (1000 - 1300 nm)	±8.0° (at 800 nm) ±8.5° (at 900 nm) ±11.5° (1000 - 1800 nm)
Optical Scan Anglec (Two-Axis Scan)	Maximum	±8.1° x ±8.1°		±8.5° x ±8.5°
	Diffraction Limited	±5.3° x ±5.3° (at 486.1 nm) ±6.3° x ±6.3° (at 587.6 nm) ±8.1° x ±8.1° (656.6 - 1100 nm)	±5.3° x ±5.3° (at 680 nm) ±6.3° x ±6.3° (at 800 nm) ±8.1° x ±8.1° (1000 - 1300 nm)	±6.0° x ±6.0° (at 900 nm) ±8.5° x ±8.5° (1000 - 1800 nm)
Spot Size Plotsa		Single-Axis Scan:	Single-Axis Scan:	Single-Axis Scan:
Field of Viewd	Maximum	14.1 x 14.1 mm2, FN20 (Two-Axis Scan)
	Diffraction Limited	9.2 x 9.2 mm2, FN13 (at 486.1 nm) 11.1 x 11.1 mm2, FN15.7 (at 587.6 nm) 14.1 x 14.1 mm2, FN20 (656.5 - 1100 nm)	8.9 x 8.9 mm2, FN12.6 (at 680 nm) 9.9 x 9.9 mm2, FN14 (at 800 nm) 14.1 x 14.1 mm2, FN20 (1000 - 1300 nm)	10.5 x 10.5 mm2, FN14.8 (at 900 nm) 14.1 x 14.1 mm2, FN20 (1000 - 1800 nm)
F-Theta Distortione		<0.2%
Field Curvaturee		<500 µm		<700 µm
Axial Color		<80 µm for 450 - 1100 nm <25 µm for 680 - 1100 nm Plot:	<130 µm for 680 - 1300 nm <25 µm for 680 - 1100 nm Plot:	<250 µm (800 to 1800 nm) Plot:
f/#		12.5	12.5 (4 mm Entrance Pupil) 10 (5 mm Entrance Pupil)
Transmittance
Dimensional Specifications
Mounting Threads		External SM30 (M30.5 x 0.5)
Thread Length		3.0 mm (0.12")
Barrel Diameter		35.0 mm (1.38")
Length of Barrel		50.9 mm (2.00")

• The beam at the entrance pupil is assumed to have uniform intensity across the entrance pupil.
• Distance from Pupil Position to the Base of the Mounting Threads (See Figure G3.2)
• Defined with Respect to the Optical Axis of the Lens
• FN: Field Number, Given in mm, with Field of View = (FN) / (Lens Magnification)
• At the Center Wavelength, Unless Noted Otherwise

Definitions of Key Parameters

Entrance Pupil Size (EP): If a single galvo mirror is used, the Entrance Pupil (EP), also known as the back aperture, is located at the pivot point of the mirror. When two mirrors are used, the EP is located between the mirrors. The size of the EP specifies the diameter of the collimated laser beam that will maximize the resolution of the imaging system.

Scanning Distance (SD): The Scanning Distance (SD) is the distance between the aperture plane, where the EP lies, and the back mounting plane of the objective, which is defined as the base of the mounting threads. For these lenses, the mounting plane is the shoulder adjacent to the threads, or thread mounting plane, of the lens. When two galvo mirrors are used, the aperture plane occurs midway between the two mirrors. When one galvo mirror is used, the pivot point of the single mirror coincides with the aperture plane. See the LSM Info tab for more details.

Scan Angle (SA): After being routed from the galvo mirror(s) the laser beam is incident on the lens at an angle. This angle, measured with respect to the optical axis of the lens, is the scan angle. When listed in the specifications table, it indicates the range of maximum allowed scan angles.

Parfocal Distance (PD): The distance from the scan lens mounting plane to the front focal plane of the scan lens.

Working Distance (WD or LWD): The distance between the tip of the scan lens housing and the front focal plane of the scan lens.

Field of View (FOV): The FOV is the maximum size of the area on the sample that can be imaged with a resolution equal to or better than the stated resolution of the scan lens. This assumes the proper utilization of the scan lenses in the optical system. During operation, the position of the spot is scanned over the FOV.

Spot Size Data

Spot sizes formed in the intermediate image plane are typically of more interest to OCT system design than to other laser scanning microscopy system designs. This is because in most OCT systems the image plane is also the sample plane. In other laser scanning applications, scan lenses are routinely paired with tube lenses and the image plane of the scan lens does not coincide with the sample plane.

Spot size plots for these lenses may be viewed by clicking on the blue icons, , in Tables G3.1 and G3.3. Spot size data are calculated for simulated single-axis scans with a single galvo mirror. The location of the entrance pupil, which is positioned at the aperture plane, varies depending on whether one or two mirrors are used. When a single galvo mirror is used, the scan plane falls at the pivot point of the mirror, as is shown in Figure 6.2. When two galvo mirrors are used, the scan plane is located midway between two galvo mirrors, as is shown in Figure 6.3.

The 2D single-axis scan graph contains several curves within the operating wavelength range. Together, the curves illustrate the dependence of spot size on wavelength. The single-axis scan graphs plot spot size and spot position over a one-dimensional range of scan angles; the spot positions scan a line through the image plane. Because the scan plane is not located in the same place for single-axis and two-axis scans, the data in the single-axis plot is not an exact cross section of a two-axis scan. The single-axis scan data were simulated using a system focused at the center wavelength of the lens; the system was not individually focused at each of the wavelengths shown.

Click for Details
Figure 497B  CSA3000 Used to Mount a Custom Epi-Illuminator and Widefield Viewing Apparatus with a sCMOS Camera

Click for Details
Figure 497A  CSA3010 Used to Mount a Custom Epi-Illuminator and Widefield Viewing Apparatus with a sCMOS Camera

• Male D1N Dovetail on Bottom for Attachment to DIY Cerna Microscope Bodies
• Available in Two Sizes in Imperial and Metric Versions:
  • Imperial: 14.00" x 11.00" or 18.00" x 4.60"
  • Metric: 350.0 mm x 275.0 mm or 450.0 mm x 116.8 mm
• 1/4"-20 or M6 x 1.0 Mounting Holes

Click to Enlarge
Figure 497C  Each breadboard has a male D1N dovetail on the bottom.

These black-anodized aluminum breadboard tops support user-designed widefield viewing apparatuses, epi-illumination pathways, and laser scanning pathways on top of upright Cerna microscopes. Each contains a Ø1.5" (Ø38.1 mm) through hole that is centered on a male D1N dovetail. This dovetail allows the breadboard to be connected directly to the epi-illumination arm of the microscope body, and it can also be used to stack the breadboard on top of an epi-illumination module. Additional details on the dovetail are available in the Microscope Dovetails tab.

The breadboards are available in two sizes. The larger version [Item # CSA3000(/M)] provides additional work surface, but protrudes past the sides of the epi-illumination arm, which may restrict approach angles around the objective for micromanipulators. The smaller version [Item # CSA3010(/M)] does not restrict approach angles and also has eight 4-40 taps around the Ø1.5" through hole for 30 mm and 60 mm cage systems.

In configurations where the breadboard is mounted directly on top of the epi-illumination arm, four M4 counterbores can be used to provide additional mounting stability.

Item #	CSA3000	CSA3000/M	CSA3010	CSA3010/M
Dimensions (L x W)	14.00" x 11.00"	350.0 mm x 275.0 mm	18.00" x 4.60"	450.0 mm x 116.8 mm
Breadboard Thickness	1/2"	12.7 mm	1/2"	12.7 mm
Hole Size and Spacing	1/4"-20 Tapped Holes on 1" Centers	M6 x 1.0 Tapped Holes on 25 mm Centers	1/4"-20 Tapped Holes on 1" Centers	M6 x 1.0 Tapped Holes on 25 mm Centers
Number of Tapped Holes	153	153	87	89
Cage System Compatibility	Four 4-40 Taps for 30 mm Cage Systems Four 4-40 Taps for 60 mm Cage Systems
Click for Mechanical Drawing
Dovetail	Male D1N
Material	Matte Black Anodized Aluminum

Click to Enlarge

View Imperial Product List

Item #	Qty	Description
Items on Microscope Body
CEA1400	1	Cerna Microscope Body with Epi-Illumination Arm, 400 mm Rail
PLSZ	1	Motorized Module with 1" Travel, 95 mm Dovetail
PLSZ1	1	Angle Bracket for Edge-Mounted Arms
CSA1200	1	Mounting Arm for CSN1201 and CSN1202 Nosepieces
CSN1202	1	Nosepiece for 2 Objectives, M25 x 0.75 Threads
MCM301	1	Three-Channel Stepper Motor Controller for Cerna Components
Items on Bottom Optical Path
OPX2400	1	Breadboard Top with Slider, 1/4"-20 Taps, Male & Female D1N Dovetails
PFR14-P02	1	35 mm x 52 mm Protected Silver Mirror
LCP34	3	60 mm Cage Plate, SM2 Threads, 0.5" Thick, 8-32 Tap (Two SM2RR Retaining Rings Included)
LA1401	2	N-BK7 Plano-Convex Lens, Ø2", f = 60 mm, Uncoated
LA1301	1	N-BK7 Plano-Convex Lens, Ø2", f = 250 mm, Uncoated
LCP50D	1	60 mm Cage System Iris Diaphragm (Ø2.0 - Ø50.0 mm)
LCFW5A	1	60 mm Cage Filter Wheel for Five Ø2" (Ø50.8 mm) Filters, Imperial
NE2R03B	1	Unmounted Ø2" Absorptive ND Filter, Optical Density: 0.3
NE2R13B	1	Unmounted Ø2" Absorptive ND Filter, Optical Density: 1.3
SLSLLG1	1	Liquid Light Guide Adapter for Broadband Light Sources, 185 nm - 2.1 µm
TRP057	2	Ø0.47" Pedestal Pillar Post, 8-32 Setscrew, 1/4"-20 Tap, L = 0.568"
MSC2	2	Clamping Fork for Ø12 mm Pedestal Posts, 1/4"-20 (M6) Cap Screw
ER8-P4	1	Cage Assembly Rod, 8" Long, Ø6 mm, 4 Pack
ER4-P4	1	Cage Assembly Rod, 4" Long, Ø6 mm, 4 Pack
Items on Top Optical Path
WFA2002	1	Epi-Illuminator Module for 1 Cube, Male & Female D1N Dovetails
TLV-U-MF2	1	Microscopy Cube Assembly for Olympus AX, BX2, and IX2 Microscopes
DMLP490R	1	25 mm x 36 mm Longpass Dichroic Mirror, 490 nm Cut-On
CP02F	2	SM1-Threaded 30 mm Flexure Cage Plate, 0.35" Thick, 2 Retaining Rings, Imperial
LA1172-A	1	N-BK7 Plano-Convex Lens, Ø1", f = 400 mm, AR Coating: 350 - 700 nm
LA1461-A	1	N-BK7 Plano-Convex Lens, Ø1", f = 250 mm, AR Coating: 350 - 700 nm
CP36	1	30 mm Cage Plate, Ø1.2" Double Bore for SM1 and C-Mount Lens Tubes
M455L4	1	455 nm, 1150 mW (Min) Mounted LED, 1000 mA
LAURE1	1	Cerna Trinoculars with 10X Eyepieces, Upright Image, IR Filter
Add To CartExportForward

View Metric Product List

Item #	Qty	Description
Items on Microscope Body
CEA1400	1	Cerna Microscope Body with Epi-Illumination Arm, 400 mm Rail
PLSZ	1	Motorized Module with 1" Travel, 95 mm Dovetail
PLSZ1	1	Angle Bracket for Edge-Mounted Arms
CSA1200	1	Mounting Arm for CSN1201 and CSN1202 Nosepieces
CSN1202	1	Nosepiece for 2 Objectives, M25 x 0.75 Threads
MCM301	1	Three-Channel Stepper Motor Controller for Cerna Components
Items on Bottom Optical Path
OPX2400/M	1	Breadboard Top with Slider, M6 x 1.0 Taps, Male & Female D1N Dovetails
PFR14-P02	1	35 mm x 52 mm Protected Silver Mirror
LCP34/M	3	60 mm Cage Plate, SM2 Threads, 0.5" Thick, M4 Tap (Two SM2RR Retaining Rings Included)
LA1401	2	N-BK7 Plano-Convex Lens, Ø2", f = 60 mm, Uncoated
LA1301	1	N-BK7 Plano-Convex Lens, Ø2", f = 250 mm, Uncoated
LCP50D	1	60 mm Cage System Iris Diaphragm (Ø2.0 - Ø50.0 mm)
LCFW5A/M	1	60 mm Cage Filter Wheel for Five Ø2" (Ø50.8 mm) Filters, Metric
NE2R03B	1	Unmounted Ø2" Absorptive ND Filter, Optical Density: 0.3
NE2R13B	1	Unmounted Ø2" Absorptive ND Filter, Optical Density: 1.3
SLSLLG1	1	Liquid Light Guide Adapter for Broadband Light Sources, 185 nm - 2.1 µm
TRP14/M	2	Ø12 mm Pedestal Pillar Post, M4 Setscrew, M6 Tap, L = 14.4 mm
MSC2	2	Clamping Fork for Ø12 mm Pedestal Posts, 1/4"-20 (M6) Cap Screw
ER8-P4	1	Cage Assembly Rod, 8" Long, Ø6 mm, 4 Pack
ER4-P4	1	Cage Assembly Rod, 4" Long, Ø6 mm, 4 Pack
Items on Top Optical Path
WFA2002	1	Epi-Illuminator Module for 1 Cube, Male & Female D1N Dovetails
TLV-U-MF2	1	Microscopy Cube Assembly for Olympus AX, BX2, and IX2 Microscopes
DMLP490R	1	25 mm x 36 mm Longpass Dichroic Mirror, 490 nm Cut-On
CP02F/M	2	SM1-Threaded 30 mm Flexure Cage Plate, 0.35" Thick, 2 Retaining Rings, Metric
LA1172-A	1	N-BK7 Plano-Convex Lens, Ø1", f = 400 mm, AR Coating: 350 - 700 nm
LA1461-A	1	N-BK7 Plano-Convex Lens, Ø1", f = 250 mm, AR Coating: 350 - 700 nm
CP36	1	30 mm Cage Plate, Ø1.2" Double Bore for SM1 and C-Mount Lens Tubes
M455L4	1	455 nm, 1150 mW (Min) Mounted LED, 1000 mA
LAURE1	1	Cerna Trinoculars with 10X Eyepieces, Upright Image, IR Filter
Add To CartExportForward

Figure G5.1  Here, a white-light illumination path has been connected to the OPX2400 using our 60 mm cage system, and a GFP fluorescence path has been mounted on top of the OPX2400 via our WFA2002 epi-illuminator module.

• Two-Position Slider to Combine or Switch Between DIY Optical Paths
• Slider has Internal SM2 Threads and Holds One 35 mm x 52 mm x 3 mm Optic
• Back Port has Internal SM2 Threads and Four 4-40 Taps for Our 60 mm Cage System
• Imperial and Metric Versions
  • OPX2400: 10.16" x 3.94" Breadboard with Double-Density 1/4"-20 Tapped Holes
  • OPX2400/M: 258 mm x 100 mm Breadboard with Double-Density M6 x 1.0 Tapped Holes
• Stackable Design with Female and Male D1N Dovetails on Top and Bottom, Respectively

Click to Enlarge

Figure G5.2  (a) Slider Located Above Objective
(b) Slider Not in Optical Path with Objective
The lid of the slider housing is opened by removing four cap screws with a 3 mm balldriver. The slider and the slider housing are internally SM2-threaded. Two stainless steel tracks and detents provide repeatable positioning.

The OPX2400(/M) Breadboard Top with Two-Position Slider adds a manually operated optic slider to the epi-illumination arm of a Cerna microscope body. By mounting a dichroic, beamsplitter, or mirror into the slider, users may combine or switch between widefield viewing, epi-illumination, and/or laser scanning pathways.

The optic slider has a clear aperture of Ø1.65" (Ø41.9 mm) and uses a leaf spring to retain a rectangular optic (minimum size: 34.9 mm x 51.9 mm x 2.8 mm; maximum size: 35.0 mm x 52.0 mm x 3.2 mm); the large aperture and optic size allow the entire aperture of the scan lenses above to be utilized. It has internal SM2 (2.035"-40) threads that face the back of the stationary housing, allowing a tube lens to be installed at a fixed distance from the dichroic. The back of the housing also has internal SM2 threads, as well as four 4-40 taps spaced for our 60 mm cage system.

In addition, a breadboard with sixty-eight 1/4"-20 (M6 x 1.0) through-tapped holes in a double-density hole pattern is included to support a home-built optical path. More 1/4"-20 (M6 x 1.0) tapped holes (sixteen on the imperial version and eighteen on the metric version) are located on the sides of the breadboard. Measured from the top of the breadboard, the beam height is 50.0 mm. Thorlabs manufactures Ø12 mm pedestal posts that center many of our 30 mm and 60 mm cage plates at this beam height, as illustrated in this photo, which provide structural support for large or heavy setups.

Thorlabs offers a 750 nm shortpass dichroic (Item # DMSP750B) and a protected silver mirror (Item # PFR14-P02) as stocked items. Beamsplitters and dichroics at additional cutoff wavelengths are available by contacting Tech Support. Once the optic is mounted, a 5/64" (2 mm) hex balldriver can be used to fine tune the optic slider's pitch and yaw adjusters. The slider may be locked in either position by tightening the included locking screw with a 3/32" balldriver. In Figure G5.1, the locking screw is installed in the forward position.

In laser scanning Cerna systems, we recommend attaching the tube lens using the internal SM2 threads on the slider, since this will maximize the distance available along the throat depth to mount the objective and, if desired, non-descanned detectors.

Turret Position Sensor Software

Version 4.0 (October 11, 2019)

This software package contains the installation files for the GUI interface, driver, SDK, and support documentation. The software is compatible with Windows^® 7 (64 bit) and Windows 10 (64 bit) systems.

• Holds Up to Six Filter Sets in a Removable Turret without the Need for Filter Cubes
• Stackable Design with Female and Male D1N Dovetails on Top and Bottom, Respectively
• Directly Compatible with WFA3110 DIC Analyzer
• Monitor Filter Turret Position on a PC Using Included Software Package
• Four 4-40 Taps on Back for 60 mm Cage System

This Epi-Illuminator Module positions a rotating, removable turret (Item # CSE2000W, included with CSE2000 and sold separately below) in the epi-illumination pathway of a Cerna microscope. Fluorescence filter sets, beamsplitters, and/or mirrors can be directly loaded into the turret without the need for filter cubes. Addition of the CSE2000 to a laser scanning microscope setup makes it possible to locate a region of interest with reflected light or fluorescence imaging, then perform laser-scanned measurements after switching filter sets. Turret position may be monitored remotely on a PC via included software; see the Software box to download the GUI installation files.

The module also has a slot that holds the WFA3110 DIC Analyzer, allowing it to be used in DIC-capable systems, but please note that this analyzer is only compatible with widefield DIC, not laser-scanned DIC. In addition, a tab opens and closes a shutter that blocks the optical path directed through the excitation filter.

Microscope Compatibility
With a female D1N dovetail on top and a male D1N dovetail on the bottom, these modules are designed to mate with Thorlabs' four-channel confocal microscopes, Cerna microscope bodies, the breadboard tops sold above, and other epi-illuminator modules. Additional details on dovetails are available in the Microscope Dovetails tab.

CSE2000 Epi-Illuminator Module
This stand-alone module features four 4-40 taps for 60 mm cage systems on the back side. This mechanical interface enables Thorlabs' extensive selection of 60 mm cage components to be used to build custom epi-illumination paths. To utilize the CSE2000 with an existing microscope featuring alternate dovetails, contact Tech Support for a custom solution. 

CSE2000W Turret for Six Filter Sets
One CSE2000W Turret is included with each module, and additional turrets are available separately. The turret has six positions for filter sets, with each set consisting of up to two round filters (Ø1", <5.1 mm thick) and one rectangular optic (25 mm x 36 mm, 1 ± 0.1 mm thick). These filters are secured using the included SM1RR retaining rings and leaf springs, respectively. The emission filter slots are tilted by 5° in order to reduce unwanted back reflections in the light path that exits vertically.

Click for Details
Figure G6.4  Remove the top plate from the turret to install dichroics or mirrors.

Click to Enlarge
Figure G6.3  CSE2000W Filter Turret with Top Plate

Click to Enlarge
Figure G6.2  Once the front plate is removed, the turret can be safely removed from the epi-illuminator module by way of grip holes.

Click to Enlarge
Figure G6.1  The CSE2000 Epi-Illuminator Module features 4-40 taps to connect to a 60 mm cage system.

Click for Details
Figure 597B  Here, the CSA1003 Dovetail Adapter is being used to connect a 60 mm cage system to the bottom of a WFA2002 Epi-Illuminator Module.

Click to Enlarge
Figure 597A  Here, the WFA4111 adapter is being used to mount an SM2 lens tube on top of an WFA2002 Epi-Illuminator Module.

• Extend Versatility of Thorlabs' Lens Tube and Cage Construction Systems to DIY Cerna Systems
• LCPN2: Male D1N Dovetail, Internal SM30 Threads, and 30 mm and 60 mm Cage Compatibility
• LCPN3: Male D1N Dovetail, Female D5Y Dovetail, Internal SM30 Threads, and 60 mm Cage Compatibility
• WFA4111: Male D1N Dovetail, Internal M38 x 0.5 Threads, and External SM2 Threads
• SM2A59: Male D1N Dovetail and Internal SM2 Threads
• LCPN4: Male D1N Dovetail, Internal SM2 Threads, and 60 mm Cage Compatibility
• CSA1003: Female D1N Dovetail and 60 mm Cage Compatibility

These dovetail adapters integrate the D1N dovetail on the epi-illumination arm of a Cerna microscope body with Thorlabs' SM30 (M30 x 0.5) lens tubes, SM2 (2.035"-40) lens tubes, and 30 mm and 60 mm cage systems. Additionally, the WFA4111 and SM2A59 adapters feature internal M38 x 0.5 and SM2 threads, respectively, that can directly accept one of our infinity-corrected tube lenses. We also offer the LCPN3 trinocular port adapter, designed to allow Olympus trinoculars that have a male D5Y dovetail to be used with DIY Cerna systems.

The dovetail designations are specific to Thorlabs products; see the Microscope Dovetails tab for details.

Item #	LCPN2	LCPN3	WFA4111	SM2A59	LCPN4	CSA1003
Dovetaila	Male D1N	Male D1N Female D5Y	Male D1N			Female D1N
Threading	Internal SM30b		Internal M38 x 0.5c External SM2	Internal SM2d	Internal SM2e	None
Internal Thread Depth	0.76" (19.3 mm)	0.48" (12.2 mm)	0.53" (13.4 mm)	0.15" (3.8 mm)	0.45" (11.4 mm)	-
Cage Compatibility	30 mm Cage System (4-40 Tapf, 4 Places) 60 mm Cage System (Ø6 mm Bore, 4 Places)	60 mm Cage System (Ø6 mm Bore, 4 Places)	Noneg		60 mm Cage System (Ø6 mm Bore, 4 Places)
Clear Aperture	Ø1.10" (27.9 mm)		Ø1.47" (37.0 mm)	Ø1.69" (43.0 mm)	Ø1.74" (44.3 mm)	Ø1.50" (38.1 mm)
Adapter Profile (Click for Drawing)
Material	Black-Anodized Aluminum

• Additional information on dovetails is available in the Microscope Dovetails tab.
• This internal SM30 threading is compatible with our SM30RR retaining rings. Two SM30RR retaining rings are included.
• This internal M38 x 0.5 threading is compatible with our SM38RR retaining rings.
• This internal threading is not deep enough for mounting optics.
• This internal SM2 threading is compatible with our SM2RR retaining rings. One SM2RR retaining ring is included.
• These tapped holes are on the side opposite the dovetail only.
• An SM2-threaded cage plate can be used to convert between SM2 lens tubes and 60 mm cage systems.