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Laser Scanning for DIY Cerna® Systems


  • Build or Modify a Laser Scanning System with Various Optical and Mechanical Components
  • Support for Photostimulation, Confocal, and Multiphoton Imaging

TTL200MP

Laser Scanning
Tube Lens

CSE2000

Epi-Illuminator Module
with Turret

DIY Laser Scanning Cerna® Microscope System

LSK-GR08

Galvo-Resonant Scan Head

Related Items


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Laser-Scanning Cerna
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DIY Cerna Laser Scanning System using LSKGG4 Galvo-Galvo Scanner, OPX2400 Breadboard Top with Two-Position Slider, SL50-CLS2 Scan Lens, and WFA2001 Epi-Illuminator Module

Cerna Brochure PDF

Features

  • Galvo-Galvo and Galvo-Resonant Scan Head Options
  • Telecentric Scan and Tube Lenses with AR Coatings for Single-, Two-, and Three-Photon Excitation
  • Breadboard Tops With or Without Two-Position Slider for Combining or Switching Between Optical Paths
    • Epi-Illuminator Module with Turret for Six Filter Sets and 60 mm Cage Compatibility
    • Dovetail Adapters for Epi-Illumination Arm of Cerna® Microscope Body
    • Compatible with Thorlabs' Laser Sources, Detectors, Dichroics, Objectives, and Pinholes

    The items on this webpage are used to construct DIY Cerna systems that are capable of laser scanning microscopy. Other components will be needed to complete a DIY Cerna scanning system, such as laser sources, detectors, dichroics, objectives, and pinholes; these are not listed here as their choice depends on the specific experimental need.

    Thorlabs provides laser scanning kits for either high-speed XY scanning or custom scanning shapes. The LSKGG4(/M) Galvo-Galvo Scanner uses two galvo scan mirrors to trace a scan geometry supplied by the user, while the LSK-GR08(/M) and LSK-GR12(/M) Galvo-Resonant Scanners use one galvo scan mirror and one resonant scan mirror to scan at high speeds. Every laser scanning kit utilizes two mirrors with a protected silver coating for high reflectance of visible and NIR laser beams. Each scan head includes mounting features for Thorlabs' SM1 (1.035"-40) and SM05 (0.535"-40) threading, and 30 mm cage construction systems. See the Galvo vs Resonant tab for a features comparison between galvo and resonant scanning.

    Unleashed

    Share Your Work With Us!

    Have you built a unique setup using DIY Cerna components? Send a picture to ImagingTechSupport@thorlabs.com! Our customers often inform our engineering efforts and inspire us to make new products and improvements for the entire community. We'd love to hear from you.

    In addition, Thorlabs has engineered telecentric scan and tube lenses that are fully corrected and AR coated for multiple broadband wavelength ranges: 400 - 1300 nm, 450 - 1100 nm, 680 - 1300 nm, or 900 - 1900 nm. Identical to the optics used in our Bergamo® II multiphoton microscopes and complete Cerna-based confocal systems, these scan and tube lenses support field numbers up to 20 (i.e., up to a Ø20 mm entrance pupil at the objective), making them able to capture large-area images. Our scan lenses have external SM30 (M30 x 0.5) threads for mounting, while our tube lenses have internal and/or external SM2 (2.035"-40) threads.

    To couple the laser path into the objective, we offer breadboard tops with 1/4"-20 (M6 x 1.0) tapped holes, with or without a two-position optic slider; the large aperture of the optic slider is ideal for applications requiring large area scanning. For pairing this imaging modality with epi-fluorescence or photoactivation, the CSE2000 epi-illuminator module holds six filter sets and has four 4-40 taps for our 60 mm cage system. For custom builds, we offer D1N dovetail adapters that provide connections from the top of the epi-illumination arm to our SM30, 30 mm cage, and 60 mm cage construction systems. We also have available the LCPN3 trinocular port adapter, designed to allow Olympus trinoculars that have a male D5Y dovetail to be used with DIY Cerna systems. 

    Laser-Scanning Beam Diagram Using Cerna Components
    Click on Each Component to See Its Web Presentation

    OPX2400 or CSE2000OPX2400 or CSE2000CSE2000Scan LensTube LensTube LensScan LensGalvo-Galvo ScannerGalvo-Galvo ScannerMicroscope Objective
    Scanning Methodologies
    Galvanometer-Based Scanning Advantage: Flexible Scan Positioning
    Disadvantage: Slow
    Resonant
    Scanning
    Advantage: Scan Speed
    Disadvantage: Unable to Adjust Center of Resonant Scan Area; Nonlinear Dwell Time

    Galvo Versus Resonant Scanning

    Galvo and resonant scanning provide orthogonal advantages due to their respective scanning mechanisms. As a result, it is important to choose the scanning methodology that is ideal for the intended application. For instance, galvo-galvo scanning is optimal for user-defined scanning paths and shapes, while galvo-resonant scanning is optimal for high-speed scans.

    Galvanometer-based scanning uses a galvanometric mirror steered by an assembly system of a servo-controlled motor and a positioning actuator. The angle of the mirror is tightly controlled in the system and can be controlled via an external signal for accurate positioning and scanning, providing the flexibility for user-defined scan lines and areas. Due to inertia, the velocity of a galvo scanner has a low upper limit.

    While resonant scanning also utilizes a galvanometric mirror, the scanning method differs significantly. In a manner similar to that of a tuning fork, the mirror assembly is driven at its resonant frequency in order to reach over 10X faster scan speeds than that of galvo scanning. The speed of the resonant galvanometric mirror varies sinusoidally with the scanning angle in order to maintain smooth motion over the course of the scan, which results in longer dwell times at the edges of the FOV and shorter dwell times at the center of the scan. The combination of this effect and the fixed center position and frequency of the resonant scanner results in an inability to scan complex patterns or adjust the scanning speed. The only user-variable parameter of the resonant scan mirror is the maximum scan angle.

    Thorlabs Dovetail Referencea
    Type Shape Outer Dimension Angle
    95 mm Linear 95 mm 45°
    D1N Circular Ø2.018" 60°
    D2Nb Circular Ø1.50" 90°
    D2NBb Circular Ø1.50" 90°
    D3N Circular Ø45 mm 70°
    D5N Circular Ø1.58" 90°
    D6N Circular Ø1.90" 90°
    D7N Circular Ø2.05" 90°
    D1T Circular Ø1.50" 60°
    D3T Circular Ø1.65" 90°
    D1Y Circular Ø107 mm 60°
    D2Y Circular Ø2.32" 50°
    D3Y Circular Ø1.75" 90°
    D4Y Circular Ø56 mm 60°
    D5Y Circular Ø46 mm 60°
    D6Y Circular Ø41.9 mm 45°
    D1Z Circular Ø54 mm 60°
    D2Z Circular Ø57 mm 60°
    D3Z Circular Ø54 mm 45°
    • These dovetail designations are specific to Thorlabs products and are not used by other microscope manufacturers.
    • D2N and D2NB dovetails have the same outer diameter and angle, as defined by the drawings below. The D2N designation does not specify a height. The D2NB designation specifies a dovetail height of 0.40" (10.2 mm).
    Mating Circular Dovetails
    Click to Enlarge

    This photo shows the male D1N dovetail on the trinoculars next to the female D1N dovetail on the epi-illumination arm.
    Mating Linear Dovetails
    Click to Enlarge

    This photo shows the male 95 mm dovetail on the microscope body and the female 95 mm dovetail on the CSA1002 Fixed Arm.

    Introduction to Microscope Dovetails

    Dovetails are used for mechanical mating and optical port alignment of microscope components. Components are connected by inserting one dovetail into another, then tightening one or more locking setscrews on the female dovetail. Dovetails come in two shapes: linear and circular. Linear dovetails allow the mating components to slide before being locked down, providing flexible positioning options while limiting unneeded degrees of freedom. Circular dovetails align optical ports on different components, maintaining a single optical axis with minimal user intervention.

    Thorlabs manufactures many components which use dovetails to mate with our own components or those of other manufacturers. To make it easier to identify dovetail compatibility, we have developed a set of dovetail designations. The naming convention of these designations is used only by Thorlabs and not other microscope manufacturers. The table to the right lists all the dovetails Thorlabs makes, along with their key dimensions.

    In the case of Thorlabs’ Cerna® microscopes, different dovetail types are used on different sections of the microscope to ensure that only compatible components can be mated. For example, our WFA2002 Epi-Illuminator Module has a male D1N dovetail that mates with the female D1N dovetail on the microscope body's epi-illumination arm, while the CSS2001 XY Microscopy Stage has a female D1Y dovetail that mates with the male D1Y dovetail on the CSA1051 Mounting Arm.

    To learn which dovetail type(s) are on a particular component, consult its mechanical drawing, available by clicking on the red Docs icon (Docs Icon) below. For adapters with a female dovetail, the drawing also indicates the size of the hex key needed for the locking setscrew(s). It is important to note that mechanical compatibility does not ensure optical compatibility. Information on optical compatibility is available from Thorlabs' web presentations.

    For customers interested in machining their own dovetails, the table to the right gives the outer diameter and angle (as defined by the drawings below) of each Thorlabs dovetail designation. However, the dovetail's height must be determined by the user, and for circular dovetails, the user must also determine the inner diameter and bore diameter. These quantities can vary for dovetails of the same type. One can use the intended mating part to verify compatibility.

    In order to reduce wear and simplify connections, dovetails are often machined with chamfers, recesses, and other mechanical features. Some examples of these variations are shown by the drawings below.

    Male Microscope Dovetails
    Click to Enlarge

    Two examples of how circular male dovetails can be manufactured.
    Female Microscope Dovetails
    Click to Enlarge

    Two examples of how circular female dovetails can be manufactured.

    Standard Mechanical Interfaces on DIY Cerna® Components

    The table below gives the dovetail, optical component threads, and cage system interfaces that are present on each DIY Cerna component. If a DIY Cerna component does not have one of the standard interfaces in the table, it is not listed here. Please note that mechanical compatibility does not ensure optical compatibility. Information on optical compatibility is available from Thorlabs' web presentations.

    Item # Microscope Dovetails Optical Component Threadsa Cage Systemsb
    95 mm D1N D2N D2NB D3N D5N D1T D3T D1Y D5Y C-Mountc
    (1.00"-32)
    SM1d
    (1.035"-40)
    SM30
    (M30.5x0.5)
    SM2e
    (2.035"-40)
    30 mmd 60 mme
    2SCM1-DC Internal & External Internal Yes Yes
    BSA2000f Female
    CEA1350 Male Female Yes
    CEA1400 Male Female Yes
    CEA1500 Male Female Yes
    CEA1600 Male Female Yes
    CFB1500 Male
    CSA1000 Female
    CSA1001 Female Internal Yes
    CSA1002 Female Internal Yes
    CSA1003 Female Yes
    CSA1051 Female Male
    CSA1200f,g Yes
    CSA1400f Female Yes
    CSA1500f,h
    CSA2000f Female Internal Yes
    CSA2001 Female External
    CSA2100f Internal Yes
    CSA3000(/M) Male
    CSA3010(/M) Male Yes Yes
    Item # 95 mm D1N D2N D2NB D3N D5N D1T D3T D1Y D5Y C-Mount SM1 SM30 SM2 30 mm 60 mm
    CSC1001 Male
    CSC1002 Male
    CSC1003 Male
    CSC2001 Male
    CSD1001 Male & Female Female
    CSD1002 Male & Female External
    CSE2000 Male & Female Yes
    CSE2100 Male & Female Female Internal Yes Yes
    CSE2200 Male & Female Female Internal Yes Yes
    CSN100f,i Yes
    CSN200i Male
    CSN210i Male
    CSN500j Male
    CSN510k Male
    CSN1201g,i
    CSN1202g,j
    CSS2001 Female
    LAURE1 Male Female
    LAURE2 Male Female
    LCPN1 Male Internal Yes Yes
    LCPN2 Male Internal Yes Yes
    LCPN3 Male Female Internal Yes
    Item # 95 mm D1N D2N D2NB D3N D5N D1T D3T D1Y D5Y C-Mount SM1 SM30 SM2 30 mm 60 mm
    OPX2400(/M) Male & Female Internal Yes
    SM1A58 Male Male Internal External Yes
    SM2A56 Male External
    TC1X Male
    WFA0150 Female
    WFA1000 Yes
    WFA1010 Internal Yes
    WFA1020 Internal Yes
    WFA1051 Internal Yes
    WFA1100 Yes
    WFA2001 Male & Female Internal & External
    WFA2002 Male & Female Internal Yes
    WFA4000 Male Female
    WFA4001 Male Female
    WFA4002 Male Female
    WFA4003 Male Female
    WFA4100 Male External Internal
    WFA4101 Male External Internal
    WFA4102 Male External Internal
    WFA4105 Male External
    WFA4106 Male External
    WFA4107 Male External
    WFA4108 Male External
    WFA4109 Male External
    WFA4110 Male External
    WFA4111 Male External
    WFA4112 Male External
    Item # 95 mm D1N D2N D2NB D3N D5N D1T D3T D1Y D5Y C-Mount SM1 SM30 SM2 30 mm 60 mm
    XT95RC1(/M) Female
    XT95RC2(/M) Female
    XT95RC3(/M) Female
    XT95RC4(/M) Female
    XT95P12(/M) Female
    ZFM1020 Female
    ZFM1030 Female
    ZFM2020 Female
    ZFM2030 Female
    • Thorlabs' optical component thread adapters can be used to convert between C-Mount threads, SM1 threads, SM2 threads, and virtually every other optical thread standard.
    • Our cage system size adapters and drop-in adapter can be used to convert between 16 mm, 30 mm, and 60 mm cage systems.
    • C-Mount and CS-Mount standards feature the same 1.00"-32 threads, but C-Mounts have a 5 mm longer flange-to-sensor distance.
    • Our 30 mm cage plates can convert between SM1 lens tubes and 30 mm cage systems.
    • Our 60 mm cage plates can convert between SM2 lens tubes and 60 mm cage systems.
    • Attach to a ZFM focusing module to add a female 95 mm dovetail.
    • The CSA1200 mounting arm is compatible with the CSN1201 and CSN1202 nosepieces.
    • This blank arm is designed for custom DIY machining for non-standard components, threads, and bores..
    • This nosepiece directly accepts M32 x 0.75 objective threads.
    • This nosepiece directly accepts M25 x 0.75 objective threads.
    • This nosepiece directly accepts RMS (0.800"-36) objective threads.

    Cerna® microscopes support several imaging modalities, including epi-fluorescence, brightfield illumination, differential interference contrast (DIC) imaging, and Dodt gradient contrast imaging. Each of these methods requires different accessories and confers different advantages to the microscopist, as described below.

    Onion Mitosis
    Click to Enlarge

    Epi-Fluorescence Image of Mouse Kidney with Multiple Labels

    Epi-Fluorescence
    Epi-fluorescence makes use of fluorescent labels and intrinsic fluorescence in a specimen to identify sample features. To create an epi-fluorescence image, light that has been passed through an excitation filter is directed through an objective and absorbed by a sample. This excitation causes fluorophores within the sample to emit light of a longer wavelength (i.e., lower energy) than the excitation light. Some of this emitted light is collected by the objective, which helps direct the emission onto a camera for observation. Additional details on this imaging modality are available here.

    For performing epi-fluorescence measurements in DIY Cerna systems, we offer a range of widefield viewing and epi-illumination accessories, as well as fluorescence filter sets targeted at common fluorophores.

     

    Onion Mitosis
    Click for Details

    Brightfield Image of Onion Mitosis

    Brightfield Illumination
    Brightfield illumination is the simplest method of trans-illumination. In this modality, light from an illumination source is collected by a condenser and passed through a sample, which is observed by its effect on the intensity of the transmitted light. Brightfield illumination only requires an illumination source (i.e., an illumination kit) and a condenser to be attached to a DIY Cerna system.

     

    Buttercup Root
    Click to Enlarge

    DIC Image of a Buttercup Root

    DIC Imaging
    In differential interference contrast (DIC) imaging, light transmitted through the sample is manipulated by a number of polarization optics. Light from the illumination source is polarized and then split into two orthogonally polarized beams before it reaches the sample. Small differences in the optical path length experienced by the two beams cause interference when the beams are recombined, providing enhanced contrast for sample features that would be transparent under brightfield illumination. In addition to an illumination source and a condenser, DIC imaging requires several additional optical elements: a DIC polarizer, a condenser prism, an objective prism, and an analyzer.

     

    Mouse Retina
    Click to Enlarge

    Dodt Contrast Image of a Mouse Retina

    Dodt Contrast
    Dodt gradient contrast, also known more simply as Dodt contrast, can be understood as an improvement upon oblique illumination. Both methods use a mask to generate an illumination gradient, but in Dodt contrast, the mask occurs much earlier in the optical path. This configuration improves the image contrast to a point where it is comparable to that obtained using DIC.

    The Dodt illumination gradient is generated using a specially shaped quarter annulus and diffusers, and reveals thickness changes in a sample over the field of view. Compared to brightfield illumination, Dodt contrast offers improved resolution of sample features, and compared to DIC, it allows thicker samples to be studied. Thorlabs manufactures a pre-configured, pre-aligned illumination module for Dodt contrast that generates the desired gradient; it requires an illumination source and a condenser for operation.

     

    Mouse Retina
    Click to Enlarge

    Laser Scanned Image of a Flower Bud

    Laser Scanning
    Like epi-fluorescence, laser scanning makes use of fluorescent labels and intrinsic fluorescence in a specimen to identify sample features. Unlike epi-fluorescence, laser scanning is able to resolve thin individual planes relatively deep into a thick sample, enabling 3D volumetric images and opening the door to in vivo studies.

    Laser scanning techniques (e.g., multiphoton and confocal microscopy) rely upon the coherence of laser beams to provide significantly improved axial resolution. In confocal microscopy, a pinhole eliminates the out-of-focus light that would reduce the axial resolution (as it does in epi-fluorescence), while in multiphoton microscopy, the necessity of two- or three-photon absorption by the fluorophore, a low-probability event, effectively creates optical sections.

    Additional details are available at our laser scanning microscopy tutorial.

    Scan lenses are used in a variety of laser imaging systems, including confocal laser scanning microscopy and multiphoton imaging systems. In these applications, a laser beam incident on the back aperture (entrance pupil) of the lens is scanned through a range of angles. This translates the position of the spot formed in the image plane across the lens' field of view. In the case of non-telecentric lenses, this approach to scanning the focal spot through the image plane would introduce severe aberrations that would significantly degrade the quality of the resulting image. Telecentric scan lenses are designed to create a uniform spot size in the image plane at every scan position, which allows a high-quality image of the sample to be formed.

    In general, laser scanning microscopy systems pair a scan lens with a tube lens to create an infinity-corrected optical system. The CLS-SL, SL50-CLS2, SL50-2P2, and SL50-3P scan lenses were optimized for use in Thorlabs' confocal laser scanning and multiphoton microscopy systems. (In most OCT systems, the scan lens is designed to be used without a tube lens.)

    Scan and Tube Lens Integration
    Click to Enlarge

    The tube and scan lens schematic above shows the lens spacing for the SL50-2P2 scan lens used with a 200 mm focal length telecentric tube lens. Note that for the SL50-CLS2, the entrance pupil at the scan plane is a maximum of Ø4 mm.

    Scan Lenses Implemented in General Laser Scanning Microscopy Applications

    The image to the right shows the proper spacing of the scan and tube lenses for laser scanning microscopy. The scanning mirror, which is located at the left of the image at the scan plane, directs the laser beam through the scan lens. The angle at which the laser beam is incident on the scan lens determines the position of the focal spot in the intermediate image plane, which is located between the scan lens and the tube lens. The tube lens is positioned so that it collects and collimates the light (the focus is at infinity). The collimated light is collected by the objective, which brings it to a focus on the sample plane. Light scattered or emitted from the sample plane is collected by the objective and directed to a detector. The image below and to the left shows a CLS-SL scan lens paired with a tube lens; clicking on the image shows the correct spacing for using the CLS-SL with the TTL200 series and ITL200 tube lenses

    An attractive feature of this optical system design is the collimated light that is produced as a result of pairing the scan lens with the tube lens. With the light from the tube lens focusing at infinity, it is possible to move the position of the objective with respect to the tube lens without impacting the image quality at the sample plane. This imparts considerable flexibility to the design of the optical system. If no tube lens were used, the scan lens would also function as the objective and the intermediate image plane would become the sample plane. It would not be possible to move the image plane much with respect to the scan lens while maintaining image quality.

    The image below and to the right shows the relationship between the scan distance and the objective distance. In a perfect 4f optical system (using the CLS-SL as an example), d1 = 52 mm (minimum scan distance) and d2 = f2. However, in many practical cases the system is slightly deviated from this perfect alignment. For instance, in many commercial microscopes, the objective distance (d2) is not the same as the focal length (f2), so there may be a need to adjust distances. The figure below and to the right shows the scan and objective distance moved by some small distance δ1 and δ2, respectively. The relationship between these values is δd1 = -δd2*(f1/f2)2.

    CLS-SL Schematic
    Click for Details
    CLS-SL Tube Lens used with the ITL200 tube lens and an objective in a laser scanning system.
    CLS-SL Schematic
    Click to Enlarge
    A diagram showing lens placement for a general laser scanning system.

    When designing an imaging system that uses a scan lens, it is important to accommodate the design wavelength, parfocal distance, scanning distance, entrance pupil, and scan angle specifications in order to maximize the image quality. In general, the larger the input beam diameter, the smaller the focused spot size. However, due to the effects of vignetting and/or increased aberrations, the range of scan angles decreases as the diameter of the beam increases. Beams smaller than the entrance pupil specification will result in spot sizes larger than those specified, and beams with larger diameters will be clipped.

    For imaging systems with a single galvo mirror, the center of the scan lens' entrance pupil is coincident with the pivot point of the galvo mirror. When a single galvo mirror is used, the scanning distance is measured from the mounting surface of the lens to the pivot point of the mirror. This is shown in the image at bottom-left.

    If the imaging system uses two galvo mirrors (one to scan in the X direction and one to scan in the Y direction), the entrance pupil is located between the two galvo mirrors, as is shown in the image at bottom-right. The scanning distance is then the distance from the shoulder of the lens housing to the pivot point of the mirror closest to the lens (d1) plus the distance from the pivot point of that mirror to the entrance pupil (d2). It is important to minimize the distance between the two galvo mirrors, because when the entrance pupil and beam steering pivot point are not coincident, the quality of the image is degraded. This is principally due to the variation in the optical path length as the beam is scanned over the sample. Below are schematics for an imaging system containing one and two galvo mirrors.

    1D Galvo Mirror Schematic
    When one galvo mirror is used, the entrance pupil
    is located at the pivot point of the mirror.
    2D Galvo Mirror Schematic
    When two galvo mirrors are used, the entrance
    pupil is located between the mirrors.

    Building a Cerna® Microscope

    The Cerna microscopy platform's large working volume and system of dovetails make it straightforward to connect and position the components of the microscope. This flexibility enables simple and stable set up of a preconfigured microscope, and provides easy paths for later upgrades and modification. See below for a couple examples of the assembly of preconfigured and DIY Cerna microscopes.

    Preconfigured Microscope Kit Design and Assembly


    Walkthrough of Cerna® Microscope Kit 4
    This Cerna microscope configuration is equipped with both epi- and trans-illumination modules. All Cerna preconfigured microscope kits enable individual components to be removed or substituted for complete customization.

    Microscope Kit 4 Assembly
    The D1N and D2N circular dovetails align the sample viewing and epi-illumination apparatus along the optical path. The microscope body's 95 mm linear dovetail is used to secure the objective and condenser mounts, as well as the transmitted light illumination module. The dovetail allows components to slide along the vertical rail prior to lockdown.

    DIY Cerna Design and Assembly


    Walkthrough of a DIY Microscope Configuration
    This DIY microscope uses a CSA3000 Breadboard Top, a CSA2001 Dovetail Adapter, our CSA1001 and CSA1002 Fixed Arms, and other body attachments and extensions. These components provide interfaces to our lens tube and cage construction systems, allowing the rig to incorporate two independent trans-illumination modules, a home-built epi-illumination path, and a custom sample viewing optical path.

    DIY Microscope Configuration Assembly
    The simplicity of Thorlabs optomechanical interfaces allows a custom DIY microscope to be quickly assembled and reconfigured for custom imaging applications.

    Cerna Mind Map

    Click on the different parts of the microscope to explore their functions.

    Explore the Cerna MicroscopeSample Viewing/RecordingSample MountingIllumination SourcesIllumination SourcesObjectives and MountingEpi-IlluminationEpi-IlluminationTrans-IlluminationMicroscope BodyMicroscope BodyMicroscope BodyMicroscope Body

    Elements of a Microscope

    This overview was developed to provide a general understanding of a Cerna® microscope. Click on the different portions of the microscope graphic to the right or use the links below to learn how a Cerna microscope visualizes a sample.

     

    Terminology

    Arm: Holds components in the optical path of the microscope.

    Bayonet Mount: A form of mechanical attachment with tabs on the male end that fit into L-shaped slots on the female end.

    Bellows: A tube with accordion-shaped rubber sides for a flexible, light-tight extension between the microscope body and the objective.

    Breadboard: A flat structure with regularly spaced tapped holes for DIY construction.

    Dovetail: A form of mechanical attachment for many microscopy components. A linear dovetail allows flexible positioning along one dimension before being locked down, while a circular dovetail secures the component in one position. See the Microscope Dovetails tab or here for details.

    Epi-Illumination: Illumination on the same side of the sample as the viewing apparatus. Epi-fluorescence, reflected light, and confocal microscopy are some examples of imaging modalities that utilize epi-illumination.

    Filter Cube: A cube that holds filters and other optical elements at the correct orientations for microscopy. For example, filter cubes are essential for fluorescence microscopy and reflected light microscopy.

    Köhler Illumination: A method of illumination that utilizes various optical elements to defocus and flatten the intensity of light across the field of view in the sample plane. A condenser and light collimator are necessary for this technique.

    Nosepiece: A type of arm used to hold the microscope objective in the optical path of the microscope.

    Optical Path: The path light follows through the microscope.

    Rail Height: The height of the support rail of the microscope body.

    Throat Depth: The distance from the vertical portion of the optical path to the edge of the support rail of the microscope body. The size of the throat depth, along with the working height, determine the working space available for microscopy.

    Trans-Illumination: Illumination on the opposite side of the sample as the viewing apparatus. Brightfield, differential interference contrast (DIC), Dodt gradient contrast, and darkfield microscopy are some examples of imaging modalities that utilize trans-illumination.

    Working Height: The height of the support rail of the microscope body plus the height of the base. The size of the working height, along with the throat depth, determine the working space available for microscopy.

     

    microscope bodyClick to Enlarge
    Cerna Microscope Body
    Body Height Comparison
    Click to Enlarge

    Body Details

    Microscope Body

    The microscope body provides the foundation of any Cerna microscope. The support rail utilizes 95 mm rails machined to a high angular tolerance to ensure an aligned optical path and perpendicularity with the optical table. The support rail height chosen (350 - 600 mm) determines the vertical range available for experiments and microscopy components. The 7.74" throat depth, or distance from the optical path to the support rail, provides a large working space for experiments. Components attach to the body by way of either a linear dovetail on the support rail, or a circular dovetail on the epi-illumination arm (on certain models). Please see the Microscope Dovetails tab or here for further details.

     

    microscope bodyClick to Enlarge
    Illumination with a Cerna microscope can come from above (yellow) or below (orange). Illumination sources (green) attach to either.

    Illumination

    Using the Cerna microscope body, a sample can be illuminated in two directions: from above (epi-illumination, see yellow components to the right) or from below (trans-illumination, see orange components to the right).

    Epi-illumination illuminates on the same side of the sample as the viewing apparatus; therefore, the light from the illumination source (green) and the light from the sample plane share a portion of the optical path. It is used in fluorescence, confocal, and reflected light microscopy. Epi-illumination modules, which direct and condition light along the optical path, are attached to the epi-illumination arm of the microscope body via a circular D1N dovetail (see the Microscope Dovetails tab or here for details). Multiple epi-illumination modules are available, as well as breadboard tops, which have regularly spaced tapped holes for custom designs.

    Trans-illumination illuminates from the opposite side of the sample as the viewing apparatus. Example imaging modalities include brightfield, differential interference contrast (DIC), Dodt gradient contrast, oblique, and darkfield microscopy. Trans-illumination modules, which condition light (on certain models) and direct it along the optical path, are attached to the support rail of the microscope body via a linear dovetail (see Microscope Dovetails tab or here). Please note that certain imaging modalities will require additional optics to alter the properties of the beam; these optics may be easily incorporated in the optical path via lens tubes and cage systems. In addition, Thorlabs offers condensers, which reshape input collimated light to help create optimal Köhler illumination. These attach to a mounting arm, which holds the condenser at the throat depth, or the distance from the optical path to the support rail. The arm attaches to a focusing module, used for aligning the condenser with respect to the sample and trans-illumination module.

     

    microscope bodyClick to Enlarge
    Light from the sample plane is collected through an objective (blue) and viewed using trinocs or other optical ports (pink).

    Sample Viewing/Recording

    Once illuminated, examining a sample with a microscope requires both focusing on the sample plane (see blue components to the right) and visualizing the resulting image (see pink components).

    A microscope objective collects and magnifies light from the sample plane for imaging. On the Cerna microscope, the objective is threaded onto a nosepiece, which holds the objective at the throat depth, or the distance from the optical path to the support rail of the microscope body. This nosepiece is secured to a motorized focusing module, used for focusing the objective as well as for moving it out of the way for sample handling. To ensure a light-tight path from the objective, the microscope body comes with a bellows (not pictured).

    Various modules are available for sample viewing and data collection. Trinoculars have three points of vision to view the sample directly as well as with a camera. Double camera ports redirect or split the optical path among two viewing channels. Camera tubes increase or decrease the image magnification. For data collection, Thorlabs offers both cameras and photomultiplier tubes (PMTs), the latter being necessary to detect fluorescence signals for confocal microscopy. Breadboard tops provide functionality for custom-designed data collection setups. Modules are attached to the microscope body via a circular dovetail (see the Microscope Dovetails tab or here for details).

     

    microscope bodyClick to Enlarge
    The rigid stand (purple) pictured is one of various sample mounting options available.

    Sample/Experiment Mounting

    Various sample and equipment mounting options are available to take advantage of the large working space of this microscope system. Large samples and ancillary equipment can be mounted via mounting platforms, which fit around the microscope body and utilize a breadboard design with regularly spaced tapped through holes. Small samples can be mounted on rigid stands (for example, see the purple component to the right), which have holders for different methods of sample preparation and data collection, such as slides, well plates, and petri dishes. For more traditional sample mounting, slides can also be mounted directly onto the microscope body via a manual XY stage. The rigid stands can translate by way of motorized stages (sold separately), while the mounting platforms contain built-in mechanics for motorized or manual translation. Rigid stands can also be mounted on top of the mounting platforms for independent and synchronized movement of multiple instruments, if you are interested in performing experiments simultaneously during microscopy.

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    For sample viewing, Thorlabs offers trinoculars, double camera ports, and camera tubes. Light from the sample plane can be collected via cameras, photomultiplier tubes (PMTs), or custom setups using breadboard tops. Click here for additional information about viewing samples with a Cerna microscope.

    Product Families & Web Presentations
    Sample Viewing Breadboards
    & Body Attachments
    Cameras PMTs

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    Microscope objectives are held in the optical path of the microscope via a nosepiece. Click here for additional information about viewing a sample with a Cerna microscope.

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    Large and small experiment mounting options are available to take advantage of the large working space of this microscope. Click here for additional information about mounting a sample for microscopy.

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    Thorlabs offers various light sources for epi- and trans-illumination. Please see the full web presentation of each to determine its functionality within the Cerna microscopy platform.

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    Epi-illumination illuminates the sample on the same side as the viewing apparatus. Example imaging modalities include fluorescence, confocal, and reflected light microscopy. Click here for additional information on epi-illumination with Cerna.

    Product Families & Web Presentations
    Epi-Illumination Web Presentation Body Attachments Light Sources
    Epi-Illumination Body Attachments Light Sources

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    Trans-illumination illuminates from the opposite side of the sample as the viewing apparatus. Example imaging modalities include brightfield, differential interference contrast (DIC), Dodt gradient contrast, oblique, and darkfield microscopy. Click here for additional information on trans-illumination with Cerna.

    Product Families & Web Presentations
    Brightfield Web Presentation DIC Web Presentation Dodt Web Presentation Condensers Web Presentation Condenser Mounting Web Presentation Illumination Kits Web Presentation Other Light Sources
    Brightfield DIC Dodt Condensers Condenser Mounting Illumination Kits Other Light Sources

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    The microscope body provides the foundation of any Cerna microscope. The 7.74" throat depth provides a large working space for experiments. Click here for additional information about the Cerna microscope body.

    Product Families & Web Presentations
    Microscope Body Web Presentation Microscope Body Translator
    Microscope Bodies Microscope Translator

    Posted Comments:
    wenzel.jakob  (posted 2017-11-27 10:28:38.877)
    The combination of the SL50-CLS2 scan lens and TL200-CLS2 tube lens transmit almost no light at 405 nm. Is it possible to combine TTL200MP or ITL200 tube lenses with a CLS-SL scan lens to scan a 405nm laser using the LSK-GG?
    tfrisch  (posted 2018-01-19 03:21:45.0)
    Hello, thank you for contacting Thorlabs. Yes, CLS-SL can be used with ITL200 and LSK-GG. I will reach out to you directly to discuss your application.

    Galvo-Galvo Scanner and Controller

    Galvo-Galvo Scan Distances
    Click for Details

    Cutaway View of Galvo-Galvo Scan Head
    The scan lens being used with the scan head needs to be positioned such that its entrance pupil is at the midpoint of the 4.33 mm green line.
    Key Specificationsa
    Scan Range ±7.5° Mechanical
    (±15° Optical)
    Beam Diameter
    4 mm (Max)
    Linearity 99.9%
    Repeatability 8 µradb
    Small Angle Step Responsec
    130 μs (Typical)
    Maximum Step Angled
    0.65°
    Time Delay Between Input and Output 90 μs
    Input Bandwidth 0 - 500 Hz (Max)e
    • Please see the full web presentation for a complete list of specifications.
    • The system noise is less than 16 bits over a full voltage range (-10 to 10 V).
    • Response corresponds to a 0.1° movement from 1% to 99% of its final position.
    • This is the maximum incremental movement at which the mirrors are able to settle within 130 μs. For movements greater than this, add a slope (Δv/Δt, where v is the difference between the voltages and t is the time difference) to your voltage profile. For example, for a step angle of 1.3°, the settling time is 260 μs.
    • Bandwidth measured for a ±10 V sine wave. Results vary depending on the waveform chosen.
    • Optical Scan Range of ±15° (±7.5° Mechanical Scan Range) for Beams up to Ø4 mm
    • Driven by User-Supplied DC, Sine, or Sawtooth Wave Signal
    • BNC Connectors and NI 68-Pin Connector for Position Control and Readout
    • Protected Silver Coating for High Reflectance in Visible and NIR
    • Scan Head is SM05, SM1, 30 mm Cage, and Post Mountable

    Our LSKGG4 Galvo-Galvo Scanner contains two galvo scan mirrors that deflect an incident laser beam in X and Y. Identical to the galvo-galvo scanner used in our Bergamo II multiphoton microscopes and complete Cerna-based confocal systems, each mirror has a mechanical scan range of ±7.5°, corresponding to an optical scan range of ±15°. This range is large enough to explore the entire diffraction-limited FN20 field of view of Thorlabs' SL50 scan lenses for laser scanning microscopy.

    A National Instruments (NI) 68-pin connector is available to directly control and monitor the scanner via an NI card or breakout box (not included). The beam can also be steered by supplying DC or sine wave drive signals through two BNC connectors. For sine wave signals, a maximum scan rate of 500 Hz is supported. Two additional BNC connectors provide X and Y position feedback signals. This scanner is also compatible with Vidrio's ScanImage software.

    Each scan mirror has a protected silver coating that provides high reflectance in the visible and NIR. Click here for a typical reflectance curve at a 45° AOI. Since the coating is metallic, its performance varies minimally over the ±7.5° mechanical scan range of the mirror.

    The scan head is connected to the controller using the included DB25 cable. If using the BNC input connectors to drive the galvo mirrors, a drive voltage of ±10 V at an impedance of 200 kΩ will be required for each axis. The BNC output connectors have a voltage range of ±4 V and an impedance of 2 kΩ.

    Complete specifications and usage details are available at our full web presentation.

    Mounting
    The input face of the scan head has internal SM05 (0.535"-40) threads, internal SM1 (1.035"-40) threads, and four Ø6 mm bores spaced for our 30 mm cage system, while the output face has internal SM1 threads and four 4-40 taps for our 30 mm cage system. The scan lens needs to be positioned such that its entrance pupil is at the midpoint between the galvo mirrors; when being used at the input, the distance is 27.255 mm, while at the output, the distance is 29.045 mm. For the CLS-SL Scan Lens, an LCP02(/M) Cage System Size Adapter can be used to convert the 30 mm cage system to a 60 mm cage system to mount and position this scan lens at the correct distance using a LCP8S Cage Plate. To mount an SL50-CLS2, SL50-2P2, or SL50-3P Scan Lens at the correct distance from the scanner a special adapter is available by contacting Tech Support.

    The scan head weighs 0.56 kg, so it may be useful to support it directly using a Ø1/2" post or Ø1" post. Four 1/4"-20 (M6) tapped holes are located on the bottom.

    Based on your currency / country selection, your order will ship from Newton, New Jersey  
    +1 Qty Docs Part Number - Imperial Price Available
    LSKGG4 Support Documentation
    LSKGG44 mm Galvo-Galvo Scanner and Controller, 1/4"-20 Taps
    $10,343.78
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    LSKGG4/M Support Documentation
    LSKGG4/M4 mm Galvo-Galvo Scanner and Controller, M6 Taps
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    Galvo-Resonant Scanners and Controllers

    Galvo-Resonant Scan Distances
    Click to Enlarge

    Cutaway View of Galvo-Resonant Scan Head
    The scan lens being used with the scan head needs to be positioned such that its entrance pupil is at the midpoint of the 7.13 mm green line between the resonant mirror (X) and the galvo mirror (Y).
    Item #a LSK-GR08 LSK-GR12
    Galvo Scan Range ±7.5° Mechanical (±15° Optical)
    Resonant Scan Range ±5° Mechanical
    (±10° Optical)
    ±2.5° Mechanical
    (±5° Optical)
    Beam Diameter
    5 mm (Max)
    Resonant Scanner Frequency 7910 ± 15 Hz 12000 ± 15 Hz
    Galvo BNC Input Bandwidth 0 - 500 Hz (Max)b
    • Please see the full web presentation for a complete list of specifications.
    • Bandwidth measured for a ±10 V sine wave. Results vary depending on the amplitude of the waveform.
    • 8 kHz or 12 kHz Resonant Scanner Frequency for High-Speed XY Scans
    • Control via BNC Connectors, Software, and NI 68-Pin Connector
    • Protected Silver Coating for High Reflectance in Visible and NIR
    • Scan Head is SM1, 30 mm Cage, and Post Mountable

    Our Galvo-Resonant (GR) Scanners are available with 8 kHz or 12 kHz resonant scanner frequency. Each scanner contains one resonant scan mirror and one galvo scan mirror that deflect an incident laser beam in X and Y, respectively. The resonant frequency of the resonant scan mirror enables much higher-speed scans than a galvo-galvo system. The LSK-GR08(/M) 8 kHz and LSK-GR12(/M) 12 kHz GR scanners are used in our Bergamo II multiphoton microscopes and complete Cerna-based confocal systems. In our Bergamo system, the 8 kHz GR scanner can achieve 30 fps at
    512 x 512 pixels image resolution, while the 12 kHz GR scanner can achieve 45 fps at 512 x 512 pixels image resolution.

    The beam can be steered via two BNC connectors for simple uni-directional scans. For fine tuning and bi-directional scanning functionality, we provide a GUI for Windows® to adjust the scan range of the resonant scan mirror and the sync signal offset, as well as a National Instruments (NI) 68-pin connector to directly control and monitor the galvo scan mirror via an NI card or breakout box (not included). See the full web presentation for details. Each scanner is also compatible with Vidrio's ScanImage software.

    Each scan mirror has a protected silver coating that provides high reflectance in the visible and NIR. Click here for a typical reflectance curve at a 45° AOI. Since the coating is metallic, the performance varies minimally over the scan range of the mirror. The scan head input port also features an NIR window that, when combined with a scan lens on the output port, greatly reduces the audible noise from the system.

    Mounting
    The input face of each scan head has four Ø6 mm bores spaced for our 30 mm cage system. By removing the NIR window installed on the input face using a SPW801 spanner wrench, internal SM05 (0.535"-40) and internal
    SM1 (1.035"-40) threads become accessible. The output face is equipped with internal SM1 threads and four 4-40 taps for our 30 mm cage system.

    The scan lens needs to be positioned such that its entrance pupil is at the midpoint between the galvo and resonant mirrors. The distance from the input face to the midpoint is 28.655 mm, while the distance from the output face to the midpoint is 28.965 mm. Adapters with external SM1 and internal SM30 mounting threads that position our SL50-CLS2, SL50-2P2, or SL50-3P Scan Lens at the correct distance from the scanner output port are available by contacting Tech Support.

    The scan head weighs 0.76 kg, so it may be useful to support it directly using a Ø1" post. Four 1/4"-20 (M6) tapped holes are located on the bottom.

    Based on your currency / country selection, your order will ship from Newton, New Jersey  
    +1 Qty Docs Part Number - Imperial Price Available
    LSK-GR08 Support Documentation
    LSK-GR088 kHz Galvo-Resonant Scanner and Controller, 1/4"-20 Taps
    $13,791.70
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    LSK-GR12 Support Documentation
    LSK-GR1212 kHz Galvo-Resonant Scanner and Controller, 1/4"-20 Taps
    $13,791.70
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    LSK-GR08/M Support Documentation
    LSK-GR08/M8 kHz Galvo-Resonant Scanner and Controller, M6 Taps
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    LSK-GR12/M Support Documentation
    LSK-GR12/M12 kHz Galvo-Resonant Scanner and Controller, M6 Taps
    $13,791.70
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    Telecentric Scan and Tube Lenses

    • Telecentric Multiphoton Tube Lens, and Telecentric Scanning and Tube Lens Pairs
    • AR Coated for Single-, Two-, or Three-Photon Excitation
      • TTL200MP: 400 - 1300 nm
      • SL50-CLS2: 450 - 1100 nm
      • SL50-2P2: 680 - 1300 nm
      • SL50-3P: 900 - 1900 nm
    • Telecentric Design Provides Constant Spot Sizes Across the Field of View
    • Diffraction-Limited Field of View up to FN20 at Objective Plane, Depending Upon Wavelength
    • Each Scan and Tube Lens is Individually Corrected for Aberrations
    • Optic Designs Based on Our Bergamo II Multiphoton Microscopes and Complete Cerna-Based Confocal Systems

    Multiphoton Telecentric Tube Lens
    Thorlabs' TTL200MP infinity-corrected telecentric tube lens is optimized for multiphoton imaging and other laser scanning applications. This tube lens features diffraction-limited axial color correction and resolution over 400 - 2000 nm, and is AR-coated for visible and NIR wavelengths (400 - 1300 nm). This lens' effective focal length of 200 mm is the design focal length used by Thorlabs, Nikon, Leica, and Mitutoyo objectives. See the Complete Tube Lens Specifications box below for additional performance data.

    Due to its large design wavelength range, this tube lens can be paired with any of the scan lenses featured below.

    Telecentric Tube and Scan Lens Pairs
    These scan and tube lenses have been optimized for point-by-point (raster) laser scanning within one of three super broadband wavelength ranges: 450 - 1100 nm, 680 - 1300 nm, or 900 - 1900 nm. Optimized for single-, two-, and three-photon excitation, respectively, they support a diverse range of experimental needs, including confocal and multiphoton imaging, as well as photostimulation and uncaging. Depending upon the lens and the wavelengths used, field numbers up to 20 (i.e., an entrance pupil at the objective up to Ø20 mm) are supported, as shown by clicking on the Complete Lens Specifications boxes below. For reference, if a 20X objective is used with a lens pair that supports a field number of 20, the field of view will be Ø1 mm.

    Telecentric lens systems produce spot sizes in the sample plane that are nearly constant over the entire field of view, resulting in image resolution that varies minimally over the scanned area of the sample. Additional information on telecentricity is available from our telecentric lenses tutorial. Our scan and tube lenses are each individually corrected for aberrations, so it is also possible to use this tube lens with another aberration-corrected scan lens.

    Mounting Ideas
    Our scan lenses have external SM30 (M30 x 0.5) mounting threads on the end that should face the galvo scanning system. Please note that the scan lens needs to be positioned such that the scanning distance is at the midpoint between the galvo and resonant mirrors. Because of the 37.8 mm scanning distance, these lenses need to be placed in close proximity to the scanner. Adapters with external SM1 and internal SM30 mounting threads that position the scan lens at the correct distance from the scanner output port are available by contacting Tech Support.

    The TTL200MP tube lens has external SM2 threads on both sides that can be used to mount the tube lens into a Ø2" lens tube or a 60 mm cage plate. If using the OPX2400(/M) Breadboard Top with Two-Position Slider, the tube lens can also be threaded directly into the optic slider.

    This tube lens is engraved with an infinity symbol (∞) next to an arrow to indicate which side of the lens should face the objective (infinity space).

    The complete specifications available for each scan lens are provided in the table below, and definitions of the various parameters follow the table. Click the blue icons, info icon, to view the plots. All plots below show theoretical data.

    Scan Lens Item # SL50-CLS2 SL50-2P2 SL50-3P
    Optical Specifications
    Wavelength Range 450 - 1100 nm 680 - 1300 nm 900 - 1900 nm
    Effective Focal Length (EFL) 50 mm 50 mm 50 mm
    Entrance Pupil Diametera 4 mm 5 mm 5 mm
    Parfocal Distance 77.3 mm 77.3 mm 77.3 mm
    Lens Working Distance 26.4 mm 26.4 mm 26.4 mm
    Scanning Distanceb 37.8 ± 4 mm 37.8 ± 4 mm 37.8 ± 4 mm
    Optical Scan Anglec
    (Single-Axis Scan)
    Maximum ±11.5° ±11.5° ±11.5°
    Difffraction Limited ±7.5° (at 486.1 nm)
    ±9° (at 587.6 nm)
    ±11.5° (656.6 - 1100 nm)
    ±7.2° (at 680 nm)
    ±8° (at 800 nm)
    ±11.5° (1000 - 1300 nm)
    ±8.5° (at 900 nm)
    ±11.5° (1000 - 1800 nm)
    ±10.8° (at 1900 nm)
    Optical Scan Anglec
    (Two-Axis Scan)
    Maximum ±8.1° x ±8.1° ±8.1° x ±8.1° ±8.5° x ±8.5°
    Diffraction Limited ±5.3° x ±5.3° (at 486.1 nm)
    ±6.3° x ±6.3° (at 587.6 nm)
    ±8.1° x ±8.1° (656.6 - 1100 nm)
    ±5.3° x ±5.3° (at 680 nm)
    ±6.3° x ±6.3° (at 800 nm)
    ±8.1° x ±8.1° (1000 - 1300 nm)
    ±6.0° x ±6.0° (at 900 nm)
    ±8.5° x ±8.5° (1000 - 1800 nm)
    ±7.6° x ±7.6° (at 1900 nm)
    Spot Size Plotsa
    Single-Axis Scan: Plot Single-Axis Scan: Single-Axis Scan:
    Field of Viewe
    Maximum 14.1 x 14.1 mm2, FN20 (Two-Axis Scan) 14.1 x 14.1 mm2, FN20 (Two-Axis Scan) 14.1 x 14.1 mm2, FN20 (Two-Axis Scan)
    Diffraction Limited 9.2 x 9.2 mm2, FN13 (at 486.1 nm)
    11.1 x 11.1 mm2, FN15.7 (at 587.6 nm)
    14.1 x 14.1 mm2, FN20 (656.5 - 1100 nm)
    8.9 x 8.9 mm2, FN12.6 (at 680 nm)
    9.9 x 9.9 mm2, FN14 (at 800 nm)
    14.1 x 14.1 mm2, FN20 (1000 - 1300 nm)
    10.5 x 10.5 mm2, FN14.8 (at 900 nm)
    14.1 x 14.1 mm2, FN20 (1000 - 1800 nm)
    13.3 x 13.3 mm2, FN18.8 (at 1900 nm)
    F-Theta Distortione <0.2% <0.2% <0.2%
    Field Curvaturee <500 µm <500 µm <700 µm
    Axial Color <80 µm for 450 - 1100 nm
    <25 µm for 680 - 1100 nm
    Plot:
    <130 µm for 680 - 1300 nm
    <25 µm for 680 - 1100 nm
    Plot:
    <250 µm
    (900 to 1900 nm)
    Plot:
    f/# 12.5  12.5 (4 mm Entrance Pupil)
    10 (5 mm Entrance Pupil)
    12.5 (4 mm Entrance Pupil)
    10 (5 mm Entrance Pupil)
    Transmittance
    Dimensional Specifications
    Mounting Threads External SM30 (M30.5 x 0.5)
    Thread Length 3.0 mm (0.12")
    Barrel Diameter 35.0 mm (1.38")
    Length of Barrel 50.9 mm (2.00")
    • The beam at the entrance pupil is assumed to have uniform intensity across the entrance pupil.
    • Distance from Pupil Position to the Base of the Mounting Threads (See Diagram Above)
    • Defined with Respect to the Optical Axis of the Lens
    • FN: Field Number, Given in mm, with Field of View = (FN) / (Lens Magnification)
    • At the Center Wavelength, Unless Noted Otherwise

    Definitions of Key Parameters

    Scan Lens Schematic

      Entrance Pupil Size (EP): If a single galvo mirror is used, the Entrance Pupil (EP), also known as the back aperture, is located at the pivot point of the mirror. When two mirrors are used, the EP is located between the mirrors. The size of the EP specifies the diameter of the collimated laser beam that will maximize the resolution of the imaging system.

      Scanning Distance (SD): The Scanning Distance (SD) is the distance between the aperture plane, where the EP lies, and the back mounting plane of the objective, which is defined as the base of the mounting threads. For these lenses, the mounting plane is the shoulder adjacent to the threads, or thread mounting plane, of the lens. When two galvo mirrors are used, the aperture plane occurs midway between the two mirrors. When one galvo mirror is used, the pivot point of the single mirror coincides with the aperture plane. See the LSM Info tab for more details.

      Scan Angle (SA): After being routed from the galvo mirror(s) the laser beam is incident on the lens at an angle. This angle, measured with respect to the optical axis of the lens, is the scan angle. When listed in the specifications table, it indicates the range of maximum allowed scan angles.

      Parfocal Distance (PD): The distance from the scan lens mounting plane to the front focal plane of the scan lens.

      Working Distance (WD or LWD): The distance between the tip of the scan lens housing and the front focal plane of the scan lens.

      Field of View (FOV): The FOV is the maximum size of the area on the sample that can be imaged with a resolution equal to or better than the stated resolution of the scan lens. This assumes the proper utilization of the scan lenses in the optical system. During operation, the position of the spot is scanned over the FOV.

      Spot Size Data 

      Spot sizes formed in the intermediate image plane are typically of more interest to OCT system design than to other laser scanning microscopy system designs. This is because in most OCT systems the image plane is also the sample plane. In other laser scanning applications, scan lenses are routinely paired with tube lenses and the image plane of the scan lens does not coincide with the sample plane.

      Spot size plots for these lenses may be viewed by clicking on the blue icons, info icon, in the preceding tables. Spot size data are calculated for simulated single-axis scans with a single galvo mirror. The location of the entrance pupil, which is positioned at the aperture plane, varies depending on whether one or two mirrors are used. When a single galvo mirror is used, the scan plane falls at the pivot point of the mirror, as is shown in the left image of the LSM Info tab. When two galvo mirrors are used, the scan plane is located midway between two galvo mirrors, as is shown in the right image of the LSM Info tab.

      The 2D single-axis scan graph contains several curves within the operating wavelength range. Together, the curves illustrate the dependence of spot size on wavelength. The single-axis scan graphs plot spot size and spot position over a one-dimensional range of scan angles; the spot positions scan a line through the image plane. Because the scan plane is not located in the same place for single-axis and two-axis scans, the data in the single-axis plot is not an exact cross section of a two-axis scan. The single-axis scan data were simulated using a system focused at the center wavelength of the lens; the system was not individually focused at each of the wavelengths shown.

      Item # TTL200MP
      Design Wavelength Range
      400 - 2000 nm
      AR Coating Range 400 - 1300 nm
      Effective Focal Length 200 mm
      Working Distancea 148 mm
      Pupil Distanceb 228 mm (Telecentric)
      Exit Pupil Diameter 25 mm (Maximum)c
      f/# 10
      Field of View
      (Diffraction Limited)
      Ø22 mm
      Clear Aperture Ø36.8 mm
      Lens Design Apochromatic
      Axial Color Diffraction Limited
      F-Theta Distortion <0.2%
      Threading External SM2 Threads (Top and Bottom)
      Housing Length
      41.6 mm
      Performance Data (Click for Graph)
      Transmission Transmission Plot
      Axial Color Axial Color Plot
      RMS Wavefront Error RMS Wavefront Error Plot
      MTF
      MTF Icon Plot
      Data Excel Spreadsheet
      Zemax Black Box Files Forward
      Backward
      • Measured from the housing edge to the intermediate image plane (see the diagram below).
      • This is the optimal distance between the lens and the objective (see the diagram below).
      • Diffraction-Limited Up to Ø20 mm.
      Based on your currency / country selection, your order will ship from Newton, New Jersey  
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      TTL200MP Support Documentation
      TTL200MPLaser Scanning Tube Lens, f = 200 mm, ARC: 400 - 1300 nm
      $1,379.17
      Today
      SL50-CLS2 Support Documentation
      SL50-CLS2Scan Lens, 450 to 1100 nm, EFL=50 mm
      $3,257.18
      Today
      SL50-2P2 Support Documentation
      SL50-2P2Scan Lens, 680 to 1300 nm, EFL=50 mm
      $3,257.18
      Today
      SL50-3P Support Documentation
      SL50-3PScan Lens, 900 to 1900 nm, EFL=50 mm
      $4,367.43
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      Breadboard Tops for Microscope Bodies


      Click for Details

      CSA3000 Used to Mount a Custom Epi-Illuminator and Widefield Viewing Apparatus

      Click for Details

      CSA3010 Used to Mount a Custom Epi-Illuminator and Widefield Viewing Apparatus
      • Male D1N Dovetail on Bottom for Attachment to DIY Cerna Microscope Bodies
      • Available in Two Sizes in Imperial and Metric Versions:
        • Imperial: 14.00" x 11.00" or 18.00" x 4.60"
        • Metric: 350.0 mm x 275.0 mm or 450.0 mm x 116.8 mm
      • 1/4"-20 or M6 x 1.0 Mounting Holes
      Male D1N Dovetail
      Click to Enlarge

      Each breadboard has a male D1N dovetail on the bottom.

      These black-anodized aluminum breadboard tops support user-designed widefield viewing apparatuses, epi-illumination pathways, and laser scanning pathways on top of upright Cerna microscopes. Each contains a Ø1.5" (Ø38.1 mm) through hole that is centered on a male D1N dovetail. This dovetail allows the breadboard to be connected directly to the epi-illumination arm of the microscope body, and it can also be used to stack the breadboard on top of an epi-illumination module. Additional details on the dovetail are available in the Microscope Dovetails tab.

      The breadboards are available in two sizes. The larger version [Item # CSA3000(/M)] provides additional work surface, but protrudes past the sides of the epi-illumination arm, which may restrict approach angles around the objective for micromanipulators. The smaller version [Item # CSA3010(/M)] does not restrict approach angles and also has eight 4-40 taps around the Ø1.5" through hole for 30 mm and 60 mm cage systems.

      In configurations where the breadboard is mounted directly on top of the epi-illumination arm, four M4 counterbores can be used to provide additional mounting stability.

      Item # CSA3000 CSA3000/M CSA3010 CSA3010/M
      Dimensions (L x W) 14.00" x 11.00" 350.0 mm x 275.0 mm 18.00" x 4.60" 450.0 mm x 116.8 mm
      Breadboard Thickness 1/2" 12.7 mm 1/2" 12.7 mm
      Hole Size and Spacing 1/4"-20 Tapped Holes
      on 1" Centers
      M6 x 1.0 Tapped Holes
      on 25 mm Centers
      1/4"-20 Tapped Holes
      on 1" Centers
      M6 x 1.0 Tapped Holes
      on 25 mm Centers
      Number of Tapped Holes
      154 154 87 89
      Cage System Compatibility - Four 4-40 Taps for 30 mm Cage Systems
      Four 4-40 Taps for 60 mm Cage Systems
      Click for Mechanical Drawing
      Dovetail Male D1N
      Material Matte Black Anodized Aluminum
      Based on your currency / country selection, your order will ship from Newton, New Jersey  
      +1 Qty Docs Part Number - Imperial Price Available
      CSA3000 Support Documentation
      CSA3000Breadboard Top, 14.00" x 11.00", 1/4"-20 Taps, Male D1N Dovetail
      $737.33
      Today
      CSA3010 Support Documentation
      CSA3010Breadboard Top, 18.00" x 4.60", 1/4"-20 Taps, Male D1N Dovetail
      $896.46
      Today
      +1 Qty Docs Part Number - Metric Price Available
      CSA3000/M Support Documentation
      CSA3000/MBreadboard Top, 350.0 mm x 275.0 mm, M6 x 1.0 Taps, Male D1N Dovetail
      $737.33
      Today
      CSA3010/M Support Documentation
      CSA3010/MBreadboard Top, 450.0 mm x 116.8 mm, M6 x 1.0 Taps, Male D1N Dovetail
      $896.46
      Today

      Breadboard Top with Two-Position Slider

      Two Independent Epi-Illumination Paths
      Click to Enlarge

      View Imperial Product List
      Item #QtyDescription
      Items on Microscope Body
      CEA14001Cerna Microscope Body with Epi-Illumination Arm, 400 mm Rail
      ZFM20201Motorized Module with 1" Travel for Edge-Mounted Arms
      CSA12001Mounting Arm for CSN1201 and CSN1202 Nosepieces
      CSN12021Nosepiece for 2 Objectives, M25 x 0.75 Threads
      MCM30011Three-Channel Controller and Knob Box for 1" Cerna Travel Stages (Not Pictured)
      Items on Bottom Optical Path
      OPX24001Breadboard Top with Slider, 1/4"-20 Taps, Male & Female D1N Dovetails
      PFR14-P02135 mm x 52 mm Protected Silver Mirror
      LCP01360 mm Cage Plate, SM2 Threads, 0.5" Thick, 8-32 Tap (Two SM2RR Retaining Rings Included)
      LA14012N-BK7 Plano-Convex Lens, Ø2", f = 60.0 mm, Uncoated
      LA13011N-BK7 Plano-Convex Lens, Ø2", f = 250.0 mm, Uncoated
      LCP50S160 mm Cage System Iris Diaphragm (Ø2 - Ø50 mm)
      LCFW5160 mm Cage Filter Wheel for Five Ø2" (Ø50 mm) Filters
      NE2R03B1Unmounted Ø2" Absorptive ND Filter, Optical Density: 0.3
      NE2R13B1Unmounted Ø2" Absorptive ND Filter, Optical Density: 1.3
      FGT202001Ø2" Temperature-Balancing Filter, -160 mireds
      SLSLLG11Liquid Light Guide Adapter for Broadband Light Sources, 185 nm - 2.1 µm
      TRP0572Ø0.47" Pedestal Pillar Post, 8-32 Setscrew, 1/4"-20 Tap, L = 0.568"
      MSC22Clamping Fork for 1/4"-20 (M6) Cap Screw
      ER8-P41Cage Assembly Rod, 8" Long, Ø6 mm, 4 Pack
      ER4-P41Cage Assembly Rod, 4" Long, Ø6 mm, 4 Pack
      Items on Top Optical Path
      WFA20021Epi-Illuminator Module for 1 Cube, Male & Female D1N Dovetails
      TLV-U-MF21Microscopy Cube Assembly for Olympus AX, BX2, and IX2 Microscopes
      DMLP490R125 mm x 36 mm Longpass Dichroic Mirror, 490 nm Cut-On
      CP02F2SM1-Threaded 30 mm Flexure Cage Plate, 0.35" Thick, 2 Retaining Rings, Imperial
      LA1172-A1N-BK7 Plano-Convex Lens, Ø1", f = 400.0 mm, AR Coating: 350 - 700 nm
      LA1461-A1N-BK7 Plano-Convex Lens, Ø1", f = 250.0 mm, AR Coating: 350 - 700 nm
      CP36130 mm Cage Plate, Ø1.2" Double Bore for SM1 and C-Mount Lens Tubes
      M455L41455 nm, 1150 mW (Min) Mounted LED, 1000 mA
      LAURE11Cerna Trinoculars with 10X Eyepieces, Upright Image, IR Filter
      View Metric Product List
      Item #QtyDescription
      Items on Microscope Body
      CEA14001Cerna Microscope Body with Epi-Illumination Arm, 400 mm Rail
      ZFM20201Motorized Module with 1" Travel for Edge-Mounted Arms
      CSA12001Mounting Arm for CSN1201 and CSN1202 Nosepieces
      CSN12021Nosepiece for 2 Objectives, M25 x 0.75 Threads
      MCM30011Three-Channel Controller and Knob Box for 1" Cerna Travel Stages (Not Pictured)
      Items on Bottom Optical Path
      OPX2400/M1Breadboard Top with Slider, M6 x 1.0 Taps, Male & Female D1N Dovetails
      PFR14-P02135 mm x 52 mm Protected Silver Mirror
      LCP01/M360 mm Cage Plate, SM2 Threads, 0.5" Thick, M4 Tap (Two SM2RR Retaining Rings Included)
      LA14012N-BK7 Plano-Convex Lens, Ø2", f = 60.0 mm, Uncoated
      LA13011N-BK7 Plano-Convex Lens, Ø2", f = 250.0 mm, Uncoated
      LCP50S160 mm Cage System Iris Diaphragm (Ø2 - Ø50 mm)
      LCFW5160 mm Cage Filter Wheel for Five Ø2" (Ø50 mm) Filters
      NE2R03B1Unmounted Ø2" Absorptive ND Filter, Optical Density: 0.3
      NE2R13B1Unmounted Ø2" Absorptive ND Filter, Optical Density: 1.3
      FGT202001Ø2" Temperature-Balancing Filter, -160 mireds
      SLSLLG11Liquid Light Guide Adapter for Broadband Light Sources, 185 nm - 2.1 µm
      TRP14/M2Ø12 mm Pedestal Pillar Post, M4 Setscrew, M6 Tap, L = 14.4 mm
      MSC22Clamping Fork for 1/4"-20 (M6) Cap Screw
      ER8-P41Cage Assembly Rod, 8" Long, Ø6 mm, 4 Pack
      ER4-P41Cage Assembly Rod, 4" Long, Ø6 mm, 4 Pack
      Items on Top Optical Path
      WFA20021Epi-Illuminator Module for 1 Cube, Male & Female D1N Dovetails
      TLV-U-MF21Microscopy Cube Assembly for Olympus AX, BX2, and IX2 Microscopes
      DMLP490R125 mm x 36 mm Longpass Dichroic Mirror, 490 nm Cut-On
      CP02F/M2SM1-Threaded 30 mm Flexure Cage Plate, 0.35" Thick, 2 Retaining Rings, Metric
      LA1172-A1N-BK7 Plano-Convex Lens, Ø1", f = 400.0 mm, AR Coating: 350 - 700 nm
      LA1461-A1N-BK7 Plano-Convex Lens, Ø1", f = 250.0 mm, AR Coating: 350 - 700 nm
      CP36130 mm Cage Plate, Ø1.2" Double Bore for SM1 and C-Mount Lens Tubes
      M455L41455 nm, 1150 mW (Min) Mounted LED, 1000 mA
      LAURE11Cerna Trinoculars with 10X Eyepieces, Upright Image, IR Filter
      Here, a white-light illumination path has been connected to the OPX2400 using our 60 mm cage system, and a GFP fluorescence path has been mounted on top of the OPX2400 via our WFA2002 epi-illuminator module.
      • Two-Position Slider to Combine or Switch Between DIY Optical Paths
      • Slider has Internal SM2 Threads and Holds One 35 mm x 52 mm x 3 mm Optic
      • Back Port has Internal SM2 Threads and Four 4-40 Taps for Our 60 mm Cage System
      • Imperial and Metric Versions
        • OPX2400: 10.16" x 3.94" Breadboard with Double-Density 1/4"-20 Tapped Holes
        • OPX2400/M: 258 mm x 100 mm Breadboard with Double-Density M6 x 1.0 Tapped Holes
      • Stackable Design with Female and Male D1N Dovetails on Top and Bottom, Respectively
      Two Independent Epi-Illumination Paths
      Click to Enlarge

      Slider Located Above Objective
      Two Independent Epi-Illumination Paths
      Click to Enlarge

      Slider Not in Optical Path with Objective

      The lid of the slider housing is opened by removing four cap screws with a 3 mm balldriver. The slider and the slider housing are internally SM2-threaded. Two stainless steel tracks and detents provide repeatable positioning.

      The OPX2400(/M) Breadboard Top with Two-Position Slider adds a manually operated optic slider to the epi-illumination arm of a Cerna microscope body. By mounting a dichroic, beamsplitter, or mirror into the slider, users may combine or switch between widefield viewing, epi-illumination, and/or laser scanning pathways.

      The optic slider has a clear aperture of Ø1.65" (Ø41.9 mm) and uses a leaf spring to retain a rectangular optic (minimum size: 34.9 mm x 51.9 mm x 2.8 mm; maximum size: 35.0 mm x 52.0 mm x 3.2 mm); the large aperture and optic size allow the entire aperture of the scan lenses above to be utilized. It has internal SM2 (2.035"-40) threads that face the back of the stationary housing, allowing a tube lens to be installed at a fixed distance from the dichroic. The back of the housing also has internal SM2 threads, as well as four 4-40 taps spaced for our 60 mm cage system.

      In addition, a breadboard with sixty-eight 1/4"-20 (M6 x 1.0) through-tapped holes in a double-density hole pattern is included to support a home-built optical path. More 1/4"-20 (M6 x 1.0) tapped holes (sixteen on the imperial version and eighteen on the metric version) are located on the sides of the breadboard. Measured from the top of the breadboard, the beam height is 50.0 mm. Thorlabs manufactures Ø12 mm pedestal posts that center many of our 30 mm and 60 mm cage plates at this beam height, as illustrated in this photo, which provide structural support for large or heavy setups.

      Thorlabs offers a 750 nm shortpass dichroic (Item # DMSP750B) and a protected silver mirror (Item # PFR14-P02) as stocked items. Beamsplitters and dichroics at additional cutoff wavelengths are available by contacting Tech Support. Once the optic is mounted, a 5/64" (2 mm) hex balldriver can be used to fine tune the optic slider's pitch and yaw adjusters. The slider may be locked in either position by tightening the included locking screw with a 3/32" balldriver. In the photo to the upper right, the locking screw is installed in the forward position.

      In laser scanning Cerna systems, we recommend attaching the tube lens using the internal SM2 threads on the slider, since this will maximize the distance available along the throat depth to mount the objective and, if desired, non-descanned detectors.

      Based on your currency / country selection, your order will ship from Newton, New Jersey  
      +1 Qty Docs Part Number - Imperial Price Available
      OPX2400 Support Documentation
      OPX2400Breadboard Top with Slider, 1/4"-20 Taps, Male & Female D1N Dovetails
      $4,660.68
      Today
      +1 Qty Docs Part Number - Universal Price Available
      DMSP750B Support Documentation
      DMSP750B35 mm x 52 mm Shortpass Dichroic Mirror, 750 nm Cutoff
      $1,435.52
      Today
      PFR14-P02 Support Documentation
      PFR14-P0235 mm x 52 mm Protected Silver Mirror
      $530.45
      Today
      +1 Qty Docs Part Number - Metric Price Available
      OPX2400/M Support Documentation
      OPX2400/MBreadboard Top with Slider, M6 x 1.0 Taps, Male & Female D1N Dovetails
      $4,660.68
      Today

      Epi-Illuminator Module with Turret for Six Filter Sets

      Turret Position Sensor Software

      Version 3.2 (December 27, 2017)

      This software package contains the installation files for the GUI interface, driver, SDK, and support documentation. The software is compatible with Windows® 7 (64-bit) systems.

      Software Download
      • Holds Up to Six Filter Sets in a Removable Turret without the Need for Filter Cubes
      • Stackable Design with Female and Male D1N Dovetails on Top and Bottom, Respectively
      • Directly Compatible with WFA3110 DIC Analyzer
      • Monitor Filter Turret Position on a PC Using Included Software Package
      • Four 4-40 Taps on Back for 60 mm Cage System

      This Epi-Illuminator Module positions a rotating, removable turret (Item # CSE2000W, included with CSE2000 and sold separately below) in the epi-illumination pathway of a Cerna microscope. Fluorescence filter sets, beamsplitters, mirrors, and/or polarizing bandpass filters can be directly loaded into the turret without the need for filter cubes. Addition of the CSE2000 to a laser scanning microscope setup makes it possible to locate a region of interest with reflected light or fluorescence imaging, then perform laser-scanned measurements after switching filter sets. Turret position may be monitored remotely on a PC via included software; see the Software box at the top right to download the GUI installation files.

      The module also has a slot that holds the WFA3110 DIC Analyzer, allowing it to be used in DIC-capable systems, but please note that this analyzer is only compatible with widefield DIC, not laser-scanned DIC. In addition, a tab opens and closes a shutter that blocks the optical path directed through the excitation filter.

      Microscope Compatibility
      With a female D1N dovetail on top and a male D1N dovetail on the bottom, these modules are designed to mate with Thorlabs' confocal microscopes, Cerna microscope bodies, the breadboard tops sold above, and other epi-illuminator modules. Additional details on dovetails are available in the Microscope Dovetails tab.

      CSE2000 Epi-Illuminator Module
      This stand-alone module features four 4-40 taps for 60 mm cage systems on the back side. This mechanical interface enables Thorlabs' extensive selection of 60 mm cage components to be used to build custom epi-illumination paths. To utilize the CSE2000 with an existing microscope featuring alternate dovetails, contact Tech Support for a custom solution. 

      CSE200W Turret for Six Filter Sets
      One CSE2000W Turret is included with each module, and additional turrets are available separately. The turret has six positions for filter sets, with each set consisting of up to two round filters (Ø1", <5.1 mm thick) and one rectangular optic (25 mm x 36 mm, 1 ± 0.1 mm thick). These filters are secured using the included SM1RR retaining rings and leaf springs, respectively. The emission filter slots are tilted by 5° in order to reduce unwanted back reflections in the light path that exits vertically.


      Click for Details

      Remove the top plate from the turret to install dichroics or mirrors.


      Click to Enlarge

      CSE2000W Filter Turret with Top Plate

      Click to Enlarge

      Once the front plate is removed, the turret can be safely removed from the epi-illuminator module by way of grip holes.

      Click to Enlarge

      The CSE2000 Epi-Illuminator Module features 4-40 taps to connect to a 60 mm cage system.
      Specifications
      Based on your currency / country selection, your order will ship from Newton, New Jersey  
      +1 Qty Docs Part Number - Universal Price Available
      CSE2000 Support Documentation
      CSE2000Epi-Illuminator Module with Turret for Six Filter Sets
      $3,238.78
      Today
      CSE2000W Support Documentation
      CSE2000WTurret for Six Filter Sets
      $2,171.81
      Today

      Dovetail Adapters

      Cerna Camera Port Adapter
      Click for Details

      Here, the CSA1003 Dovetail Adapter is being used to connect a 60 mm cage system to the bottom of a WFA2002 Epi-Illuminator Module.
      Click to Enlarge

      Here, the WFA4111 adapter is being used to mount an SM2 lens tube on top of an WFA2002 Epi-Illuminator Module.
      • Extend Versatility of Thorlabs' Lens Tube and Cage Construction Systems to DIY Cerna Systems
      • WFA4111: Male D1N Dovetail and External SM2 Threads
      • LCPN2: Male D1N Dovetail, Internal SM30 Threads, and 30 mm and 60 mm Cage Compatibility
      • LCPN3: Male D1N Dovetail, Female D5Y Dovetail, Internal SM30 Threads, and 60 mm Cage Compatibility
      • CSA1003: Female D1N Dovetail and 60 mm Cage Compatibility

      These dovetail adapters integrate the D1N dovetail on the epi-illumination arm of a Cerna microscope body with Thorlabs' SM30 (M30 x 0.5) lens tubes, SM2 (2.035"-40) lens tubes, and 30 mm and 60 mm cage systems. Additionally, we offer the LCPN3 trinocular port adapter, designed to allow Olympus trinoculars that have a male D5Y dovetail to be used with DIY Cerna systems.

      The dovetail designations are specific to Thorlabs products; see the Microscope Dovetails tab for details.

      Item # WFA4111 LCPN2 LCPN3 CSA1003
      Dovetaila Male D1N Male D1N Male D1N
      Female D5Y
      Female D1N
      Threading Internal M38 x 0.5b
      External SM2
      Internal SM30c Internal SM30c Noned
      Cage Compatibility Noned 30 mm Cage System
      (4-40 Tape, 4 Places)
      60 mm Cage System
      (Ø6 mm Bore, 4 Places)
      60 mm Cage System
      (Ø6 mm Bore, 4 Places)
      60 mm Cage System
      (Ø6 mm Bore, 4 Places)
      Clear Aperture Ø1.47" (37.0 mm) Ø1.10" (27.9 mm) Ø1.10" (27.9 mm) Ø1.50" (38.1 mm)
      Adapter Profile
      (Click for Drawing)
      Material Black-Anodized Aluminum
      • Additional information on dovetails is available in the Microscope Dovetails tab.
      • This internal M38 x 0.5 threading is compatible with our SM38RR retaining rings.
      • This internal SM30 threading is compatible with our SM30RR retaining rings. Two SM30RR retaining rings are included.
      • An SM2-threaded cage plate can be used to convert between SM2 lens tubes and 60 mm cage systems.
      • These tapped holes are on the side opposite the dovetail only.
      Based on your currency / country selection, your order will ship from Newton, New Jersey  
      +1 Qty Docs Part Number - Universal Price Available
      WFA4111 Support Documentation
      WFA4111Adapter with Male D1N Dovetail, External SM2 Threads, and Internal M38 x 0.5 Threads
      $318.15
      Today
      LCPN2 Support Documentation
      LCPN2Nikon Eclipse or Cerna Microscope Trinocular Adapter, Male D1N Dovetail, Internal SM30 Threads, 30 and 60 mm Cage Compatibility
      $111.39
      Today
      LCPN3 Support Documentation
      LCPN3Customer Inspired! Nikon Eclipse or Cerna Microscope Trinocular Adapter, Male D1N Dovetail, Female D5Y Dovetail, Internal SM30 Threads, 60 mm Cage Compatibility
      $73.34
      Today
      CSA1003 Support Documentation
      CSA1003Adapter with Female D1N Dovetail and Bores for 60 mm Cage System
      $264.04
      Today
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