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Body Attachments and Extensions for DIY Cerna® Systems


  • Use Thorlabs' Construction Systems with DIY Cerna® Systems
  • Lens Tube, Cage System, and Dovetail Adapters
  • Breadboard Mounting Surfaces and Motorized Z-Axis Translators

CSA3010

18.00" x 4.60" Breadboard Top

CSA1001

Fixed Arm with SM1 and 30 mm Cage Compatibility

WFA4111

SM2 to D1N Adapter

OPX2400

Breadboard Top with Two-Position Slider

Application Idea

DIY Cerna System with 18.00" x 4.60" Breadboard Top and Fixed Arms

CSA1003

60 mm Cage to D1N Adapter

Related Items


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Cerna System with Body Attachments
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This DIY Cerna® system uses several body attachments and extensions to support a home-built sample viewing and epi-illumination apparatus.
Body Height Comparison
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Our DIY Cerna Microscope Bodies with Epi-Illumination Arms offer a 95 mm wide dovetail for holding fixed and motorized arms, and a female D1N dovetail that sets a 7.74" throat depth.
Quick Links
For 95 mm Dovetail Fixed Arms
Manual Translation Arms
Motorized Translation Arms
Mounting Platforms
For Epi-Illumination Arm Breadboard Tops
Breadboard Top with Two-Position Slider
Epi-llluminator Module for One Filter Cube
For Trinocular Eyepiece Eyepiece Adapter
Dovetail Adapters D1N, D2N, D2NB, D3N, and D3T

Features

  • Fixed Arms Position Lens Tubes and Cage Systems at 7.74" Throat Depth
  • Manual and Motorized Modules Translate Lens Tubes and Cage Systems Along
    Optical Axis
  • 95 mm Rail Mounting Platforms Attach User-Designed Modules to Microscope Body
  • Breadboard Tops Support Home-Built Sample Viewing and Illumination Pathways
  • Epi-Illuminator Module Integrates Filter Cubes with SM1 and 30 mm Cage Components
  • Eyepiece Adapter for Mounting Detection Systems to Trinocular Eyepieces
  • Dovetail Adapters Connect DIY Cerna® Components to Lens Tubes and Cage Systems

The items on this webpage integrate Thorlabs' lens tube systems, cage construction systems, and other optomechanics with our Cerna microscopy platform.

We offer several types of arms that center lens tubes and/or cage construction systems along an optical axis 7.74" away from the edge of a Cerna microscope body. Some arms can be directly attached to the vertical rail to provide structural support for these construction systems, while others can be connected to a manual or motorized module to enable 1" of travel along the optical axis.

In addition, we manufacture breadboard tops with optical ports centered on a male D1N dovetail. These breadboards provide 1/4"-20 (M6 x 1.0) tapped hole arrays that mount to the female D1N dovetail on a Cerna microscope body's epi-illumination arm, which can be used to create optomechanical setups. A version with a manual two-position slider is also offered that provides the ability to select between and combine optical paths.

Unleashed

Share Your Work With Us!

Have you built a unique setup using DIY Cerna components? Send a picture to ImagingTechSupport@thorlabs.com! Our customers often inform our engineering efforts and inspire us to make new products and improvements for the entire community. We'd love to hear from you.

Our 95 mm rail mounting platforms permit user-designed modules to be mounted anywhere along the sides of the vertical rail. An epi-illuminator module that interfaces our SM1 lens tube and 30 mm cage system to a fluorescence filter cube is available. An eyepiece adapter and dovetail adapters that connect a wide range of DIY Cerna components to lens tubes and cage systems are also shown below.

The Microscope Dovetails tab gives additional information on the dovetails used throughout the Cerna microscopy platform, while the Cage Overview tab gives a brief overview of our cage construction systems.

Thorlabs Dovetail Referencea
Type Shape Outer Dimension Angle
95 mm Linear 95 mm 45°
D1N Circular Ø2.018" 60°
D2Nb Circular Ø1.50" 90°
D2NBb Circular Ø1.50" 90°
D3N Circular Ø45 mm 70°
D5N Circular Ø1.58" 90°
D6N Circular Ø1.90" 90°
D7N Circular Ø2.05" 90°
D1T Circular Ø1.50" 60°
D3T Circular Ø1.65" 90°
D1Y Circular Ø107 mm 60°
D2Y Circular Ø2.32" 50°
D3Y Circular Ø1.75" 90°
D4Y Circular Ø56 mm 60°
D5Y Circular Ø46 mm 60°
D6Y Circular Ø41.9 mm 45°
D1Z Circular Ø54 mm 60°
D2Z Circular Ø57 mm 60°
D3Z Circular Ø54 mm 45°
  • These dovetail designations are specific to Thorlabs products and are not used by other microscope manufacturers.
  • D2N and D2NB dovetails have the same outer diameter and angle, as defined by the drawings below. The D2N designation does not specify a height. The D2NB designation specifies a dovetail height of 0.40" (10.2 mm).
Mating Circular Dovetails
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This photo shows the male D1N dovetail on the trinoculars next to the female D1N dovetail on the epi-illumination arm.
Mating Linear Dovetails
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This photo shows the male 95 mm dovetail on the microscope body and the female 95 mm dovetail on the CSA1002 Fixed Arm.

Introduction to Microscope Dovetails

Dovetails are used for mechanical mating and optical port alignment of microscope components. Components are connected by inserting one dovetail into another, then tightening one or more locking setscrews on the female dovetail. Dovetails come in two shapes: linear and circular. Linear dovetails allow the mating components to slide before being locked down, providing flexible positioning options while limiting unneeded degrees of freedom. Circular dovetails align optical ports on different components, maintaining a single optical axis with minimal user intervention.

Thorlabs manufactures many components which use dovetails to mate with our own components or those of other manufacturers. To make it easier to identify dovetail compatibility, we have developed a set of dovetail designations. The naming convention of these designations is used only by Thorlabs and not other microscope manufacturers. The table to the right lists all the dovetails Thorlabs makes, along with their key dimensions.

In the case of Thorlabs’ Cerna® microscopes, different dovetail types are used on different sections of the microscope to ensure that only compatible components can be mated. For example, our WFA2002 Epi-Illuminator Module has a male D1N dovetail that mates with the female D1N dovetail on the microscope body's epi-illumination arm, while the CSS2001 XY Microscopy Stage has a female D1Y dovetail that mates with the male D1Y dovetail on the CSA1051 Mounting Arm.

To learn which dovetail type(s) are on a particular component, consult its mechanical drawing, available by clicking on the red Docs icon (Docs Icon) below. For adapters with a female dovetail, the drawing also indicates the size of the hex key needed for the locking setscrew(s). It is important to note that mechanical compatibility does not ensure optical compatibility. Information on optical compatibility is available from Thorlabs' web presentations.

For customers interested in machining their own dovetails, the table to the right gives the outer diameter and angle (as defined by the drawings below) of each Thorlabs dovetail designation. However, the dovetail's height must be determined by the user, and for circular dovetails, the user must also determine the inner diameter and bore diameter. These quantities can vary for dovetails of the same type. One can use the intended mating part to verify compatibility.

In order to reduce wear and simplify connections, dovetails are often machined with chamfers, recesses, and other mechanical features. Some examples of these variations are shown by the drawings below.

Male Microscope Dovetails
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Two examples of how circular male dovetails can be manufactured.
Female Microscope Dovetails
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Two examples of how circular female dovetails can be manufactured.

Standard Mechanical Interfaces on DIY Cerna® Components

The table below gives the dovetail, optical component threads, and cage system interfaces that are present on each DIY Cerna component. If a DIY Cerna component does not have one of the standard interfaces in the table, it is not listed here. Please note that mechanical compatibility does not ensure optical compatibility. Information on optical compatibility is available from Thorlabs' web presentations.

Item # Microscope Dovetails Optical Component Threadsa Cage Systemsb
95 mm D1N D2N D2NB D3N D5N D1T D3T D1Y D5Y C-Mountc
(1.00"-32)		 SM1d
(1.035"-40)		 SM30
(M30.5x0.5)		 SM2e
(2.035"-40)		 30 mmd 60 mme
2CM1 Internal & External Internal Yes
2CM2 Internal & External Internal Yes
BSA2000f Female
CEA1350 Male Female Yes
CEA1400 Male Female Yes
CEA1500 Male Female Yes
CEA1600 Male Female Yes
CFB1500 Male
CSA1000 Female
CSA1001 Female Internal Yes
CSA1002 Female Internal Yes
CSA1003 Female Yes
CSA1051 Female Male
CSA1200f,g Yes
CSA1400f Female Yes
CSA1500f,h
CSA2000f Female Internal Yes
CSA2001 Female External
CSA2100f Internal Yes
CSA3000(/M) Male
CSA3010(/M) Male Yes Yes
Item # 95 mm D1N D2N D2NB D3N D5N D1T D3T D1Y D5Y C-Mount SM1 SM30 SM2 30 mm 60 mm
CSC1001 Male
CSC1002 Male
CSC1003 Male
CSC2001 Male
CSD1001 Male & Female Female
CSD1002 Male & Female External
CSE2000 Male & Female Yes
CSE2100 Male & Female Female Internal Yes Yes
CSE2200 Male & Female Female Internal Yes Yes
CSN100f,i Yes
CSN200i Male
CSN210i Male
CSN500j Male
CSN510k Male
CSN1201g,i
CSN1202g,j
CSS2001 Female
LAURE1 Male Female
LAURE2 Male Female
LCPN1 Male Internal Yes Yes
LCPN2 Male Internal Yes Yes
LCPN3 Male Female Internal Yes
Item # 95 mm D1N D2N D2NB D3N D5N D1T D3T D1Y D5Y C-Mount SM1 SM30 SM2 30 mm 60 mm
OPX2400(/M) Male & Female Internal Yes
SM1A58 Male Male Internal External Yes
SM2A56 Male External
TC1X Male
WFA0150 Female
WFA1000 Yes
WFA1010 Internal Yes
WFA1020 Internal Yes
WFA1051 Internal Yes
WFA1100 Yes
WFA2001 Male & Female Internal & External
WFA2002 Male & Female Internal Yes
WFA4000 Male Female
WFA4001 Male Female
WFA4002 Male Female
WFA4003 Male Female
WFA4100 Male External Internal
WFA4101 Male External Internal
WFA4102 Male External Internal
WFA4105 Male External
WFA4106 Male External
WFA4107 Male External
WFA4108 Male External
WFA4109 Male External
WFA4110 Male External
WFA4111 Male External
WFA4112 Male External
Item # 95 mm D1N D2N D2NB D3N D5N D1T D3T D1Y D5Y C-Mount SM1 SM30 SM2 30 mm 60 mm
XT95RC1(/M) Female
XT95RC2(/M) Female
XT95RC3(/M) Female
XT95RC4(/M) Female
XT95P12(/M) Female
ZFM1020 Female
ZFM1030 Female
ZFM2020 Female
ZFM2030 Female
  • Thorlabs' optical component thread adapters can be used to convert between C-Mount threads, SM1 threads, SM2 threads, and virtually every other optical thread standard.
  • Our cage system size adapters and drop-in adapter can be used to convert between 16 mm, 30 mm, and 60 mm cage systems.
  • C-Mount and CS-Mount standards feature the same 1.00"-32 threads, but C-Mounts have a 5 mm longer flange-to-sensor distance.
  • Our 30 mm cage plates can convert between SM1 lens tubes and 30 mm cage systems.
  • Our 60 mm cage plates can convert between SM2 lens tubes and 60 mm cage systems.
  • Attach to a ZFM focusing module to add a female 95 mm dovetail.
  • The CSA1200 mounting arm is compatible with the CSN1201 and CSN1202 nosepieces.
  • This blank arm is designed for custom DIY machining for non-standard components, threads, and bores..
  • This nosepiece directly accepts M32 x 0.75 objective threads.
  • This nosepiece directly accepts M25 x 0.75 objective threads.
  • This nosepiece directly accepts RMS (0.800"-36) objective threads.

Cage System Overview

The Cage Assembly System provides a convenient way to construct large optomechanical systems with an established line of precision-machined building blocks designed for high flexibility and accurate alignment.

16 mm, 30 mm, and 60 mm Cage System Standards

Thorlabs offers three standards defined by the center-to-center spacing of the cage assembly rods (see image below). The 16 mm cage, 30 mm cage, and 60 mm cage standards are designed to accomodate Ø1/2", Ø1", and Ø2" optics, respectively. Specialized cage plates that allow smaller optics to be directly inserted into our larger cage systems are also available.

Standard Threads

The flexibility of our Cage Assembly System stems from well-defined mounting and thread standards designed to directly interface with a wide range of specialized products. The three most prevalent thread standards are our SM05 Series (0.535"-40 thread), SM1 Series (1.035"-40 thread), and SM2 Series (2.035"-40 thread), all of which were defined to house the industry's most common optic sizes. Essential building blocks, such as our popular lens tubes, directly interface to these standards.


An example of the standard cage plate measurements determining cage system compatibility.
Standard Cage System Measurements
Cage System 16 mm 30 mm 60 mm
Thread Series SM05 SM1 SM2
Rod to Rod Spacing 16 mm (0.63") 30 mm (1.18") 60 mm (2.36")
Total Length 25 mm (0.98") 41 mm (1.60") 71.1 mm (2.8")
Cage Components
Cage Rods 16 mm These rods are used to connect cage plates, optic mounts, and other components in the cage system. The SR Series Cage Rods are compatible with our 16 mm cage systems, while the 30 mm and 60 mm cage systems use ER Series Cage Rods.
30 mm
60 mm
Cage Plates 16 mm These serve as the basic building blocks for a cage system. They may have SM-threaded central bores, smooth bores sized for industry standard optics or to accommodate the outer profile of our SM Series Lens Tubes, or specialized bores for other components such as our FiberPorts.
30 mm
60 mm
Optic Mounts 16 mm Thorlabs offers fixed, kinematic, rotation, and translation mounts specifically designed for our Cage Systems.
30 mm
60 mm
Cage Cubes 16 mm These cubes are useful for housing larger optical components, such as prisms or mirrors, or optics that need to sit at an angle to the beam path, such as beamsplitters. Our cage cubes are available empty or with pre-mounted optics.
30 mm
60 mm
Post and Breadboard Mounts and Adapters Mounting options for cage systems can be found on our Cage System Construction pages. Cage Systems can be mounted either parallel or perpendicular to the table surface.
Size Adapters Cage System Size Adapters can be used to integrate components from different cage system and threading standards.
Specialized Components Thorlabs also produces specialized cage components, such as Filter Wheels, a HeNe Laser Mount, and a FiberPort Cage Plate Adapter, allowing a wide range of our products to be integrated into cage-mounted optical systems. Explore our Cage Systems Visual Navigation Guide to see the full range of Thorlabs' cage components.

Thorlabs' Threading Specifications

Thorlabs' lens tubes utilize a series of non-standard threadings. Threading specifications are given below for our SM threadings utilized in our lens tube and cage system components so that you can machine mating components to suit your application. We also offer products with C-Mount and RMS threadings, and the specifications for these threadings are also given below. Please note that other manufacturers may have different tolerances for these threads. For other thread specifications that are not listed here, please contact Tech Support.


Click to Enlarge
SM05 Threading: Ø1/2" Lens Tubes, 16 mm Cage Systems
External Thread, 0.535"-40.0, UNS-2A Internal Thread, 0.535"-40.0, UNS-2B
Max Major Diameter 0.5340" Min Major Diameter 0.5350"
Min Major Diameter 0.5289" Min Pitch Diameter 0.5188"
Max Pitch Diameter 0.5178" Max Pitch Diameter 0.5230"
Min Pitch Diameter 0.5146" Min Minor Diameter (and 83.3% of thread) 0.508"
Max Minor Diameter 0.5069" Max Minor Diameter (and 64.9% of thread) 0.514"
RMS Threading: Objective, Scan, and Tube Lenses
External Thread, 0.800"-36.0, UNS-2A Internal Thread, 0.800"-36.0, UNS-2B
Max Major Diameter 0.7989" Min Major Diameter 0.8000"
Min Major Diameter 0.7934" Min Pitch Diameter 0.7820"
Max Pitch Diameter 0.7809" Max Pitch Diameter 0.7866"
Min Pitch Diameter 0.7774" Min Minor Diameter (and 83.3% of thread) 0.770"
Max Minor Diameter 0.7688" Max Minor Diameter (and 64.9% of thread) 0.777"
C-Mount Threading: Machine Vision Lenses, CCD/CMOS Cameras
External Thread, 1.000"-32.0, UN-2A Internal Thread, 1.000"-32.0, UN-2B
Max Major Diameter 0.9989" Min Major Diameter 1.0000"
Min Major Diameter 0.9929" Min Pitch Diameter 0.9797"
Max Pitch Diameter 0.9786" Max Pitch Diameter 0.9846"
Min Pitch Diameter 0.9748" Min Minor Diameter (and 83.3% of thread) 0.966"
Max Minor Diameter 0.9651" Max Minor Diameter (and 64.9% of thread) 0.974"
SM1 Threading: Ø1" Lens Tubes, 30 mm Cage Systems
External Thread, 1.035"-40.0, UNS-2A Internal Thread, 1.035"-40.0, UNS-2B
Max Major Diameter 1.0339" Min Major Diameter 1.0350"
Min Major Diameter 1.0288" Min Pitch Diameter 1.0188"
Max Pitch Diameter 1.0177" Max Pitch Diameter 1.0234"
Min Pitch Diameter 1.0142" Min Minor Diameter (and 83.3% of thread) 1.008"
Max Minor Diameter 1.0068" Max Minor Diameter (and 64.9% of thread) 1.014"
SM30 Threading: Ø30 mm Lens Tubes
External Thread, M30.5x0.5 Internal Thread, M30.5x0.5
Max Major Diameter 30.480 mm Min Major Diameter 30.500 mm
Min Major Diameter 30.371 mm Min Pitch Diameter 30.175 mm
Max Pitch Diameter 30.155 mm Max Pitch Diameter 30.302 mm
Min Pitch Diameter 30.059 mm Min Minor Diameter (and 83.3% of thread) 29.959 mm
Max Minor Diameter 29.938 mm Max Minor Diameter (and 64.9% of thread) 30.094 mm
SM1.5 Threading: Ø1.5" Lens Tubes
External Thread, 1.535"-40, UNS-2A Internal Thread, 1.535"-40, UNS-2B
Max Major Diameter 1.5339" Min Major Diameter 1.535"
Min Major Diameter 1.5288" Min Pitch Diameter 1.5188"
Max Pitch Diameter 1.5177" Max Pitch Diameter 1.5236"
Min Pitch Diameter 1.5140" Min Minor Diameter (and 83.3% of thread) 1.508"
Max Minor Diameter 1.5068" Max Minor Diameter (and 64.9% of thread) 1.514"
SM2 Threading: Ø2" Lens Tubes, 60 mm Cage Systems
External Thread, 2.035"-40.0, UNS-2A Internal Thread, 2.035"-40.0, UNS-2B
Max Major Diameter 2.0338" Min Major Diameter 2.0350"
Min Major Diameter 2.0287" Min Pitch Diameter 2.0188"
Max Pitch Diameter 2.0176" Max Pitch Diameter 2.0239"
Min Pitch Diameter 2.0137" Min Minor Diameter (and 83.3% of thread) 2.008"
Max Minor Diameter 2.0067" Max Minor Diameter (and 64.9% of thread) 2.014"
SM3 Threading: Ø3" Lens Tubes
External Thread, 3.035"-40.0, UNS-2A Internal Thread, 3.035"-40.0, UNS-2B
Max Major Diameter 3.0337" Min Major Diameter 3.0350"
Min Major Diameter 3.0286" Min Pitch Diameter 3.0188"
Max Pitch Diameter 3.0175" Max Pitch Diameter 3.0242"
Min Pitch Diameter 3.0133" Min Minor Diameter (and 83.3% of thread) 3.008"
Max Minor Diameter 3.0066" Max Minor Diameter (and 64.9% of thread) 3.014"

Building a Cerna® Microscope

The Cerna microscopy platform's large working volume and system of dovetails make it straightforward to connect and position the components of the microscope. This flexibility enables simple and stable set up of a preconfigured microscope, and provides easy paths for later upgrades and modification. See below for a couple examples of the assembly of preconfigured and DIY Cerna microscopes.

Preconfigured Microscope Kit Design and Assembly


Walkthrough of Cerna® Microscope Kit 4
This Cerna microscope configuration is equipped with both epi- and trans-illumination modules. All Cerna preconfigured microscope kits enable individual components to be removed or substituted for complete customization.

Microscope Kit 4 Assembly
The D1N and D2N circular dovetails align the sample viewing and epi-illumination apparatus along the optical path. The microscope body's 95 mm linear dovetail is used to secure the objective mounts and condenser mounts, as well as the transmitted light illumination module. The dovetail allows components to slide along the vertical rail prior to lockdown.

DIY Cerna Design and Assembly


Walkthrough of a DIY Microscope Configuration
This DIY microscope uses a CSA3000(/M) Breadboard Top, a CSA2001 Dovetail Adapter, our CSA1001 and CSA1002 Fixed Arms, and other body attachments and extensions. These components provide interfaces to our lens tube and cage construction systems, allowing the rig to incorporate two independent trans-illumination modules, a home-built epi-illumination path, and a custom sample viewing optical path.

DIY Microscope Configuration Assembly
The simplicity of Thorlabs optomechanical interfaces allows a custom DIY microscope to be quickly assembled and reconfigured for custom imaging applications.

The Cerna® Mind Map is a visual tool that contains the complete selection of DIY Cerna components and several closely related accessories. Created as a supplement to our website, we have designed it to be printed on a single 11" x 17" sheet.

Click here or on the image below to download a printable PDF. The components shown on this webpage are in Steps 2, 4, 5, 8, 9, and 12 of the mind map.

Cerna Mind Map


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Click on the different parts of the microscope to explore their functions.

Explore the Cerna MicroscopeSample Viewing/RecordingSample MountingIllumination SourcesIllumination SourcesObjectives and MountingEpi-IlluminationEpi-IlluminationTrans-IlluminationMicroscope BodyMicroscope BodyMicroscope BodyMicroscope Body

Elements of a Microscope

This overview was developed to provide a general understanding of a Cerna® microscope. Click on the different portions of the microscope graphic to the right or use the links below to learn how a Cerna microscope visualizes a sample.

 

Terminology

Arm: Holds components in the optical path of the microscope.

Bayonet Mount: A form of mechanical attachment with tabs on the male end that fit into L-shaped slots on the female end.

Bellows: A tube with accordion-shaped rubber sides for a flexible, light-tight extension between the microscope body and the objective.

Breadboard: A flat structure with regularly spaced tapped holes for DIY construction.

Dovetail: A form of mechanical attachment for many microscopy components. A linear dovetail allows flexible positioning along one dimension before being locked down, while a circular dovetail secures the component in one position. See the Microscope Dovetails tab or here for details.

Epi-Illumination: Illumination on the same side of the sample as the viewing apparatus. Epi-fluorescence, reflected light, and confocal microscopy are some examples of imaging modalities that utilize epi-illumination.

Filter Cube: A cube that holds filters and other optical elements at the correct orientations for microscopy. For example, filter cubes are essential for fluorescence microscopy and reflected light microscopy.

Köhler Illumination: A method of illumination that utilizes various optical elements to defocus and flatten the intensity of light across the field of view in the sample plane. A condenser and light collimator are necessary for this technique.

Nosepiece: A type of arm used to hold the microscope objective in the optical path of the microscope.

Optical Path: The path light follows through the microscope.

Rail Height: The height of the support rail of the microscope body.

Throat Depth: The distance from the vertical portion of the optical path to the edge of the support rail of the microscope body. The size of the throat depth, along with the working height, determine the working space available for microscopy.

Trans-Illumination: Illumination on the opposite side of the sample as the viewing apparatus. Brightfield, differential interference contrast (DIC), Dodt gradient contrast, and darkfield microscopy are some examples of imaging modalities that utilize trans-illumination.

Working Height: The height of the support rail of the microscope body plus the height of the base. The size of the working height, along with the throat depth, determine the working space available for microscopy.

 

microscope bodyClick to Enlarge
Cerna Microscope Body
Body Height Comparison
Click to Enlarge
Body Details

Microscope Body

The microscope body provides the foundation of any Cerna microscope. The support rail utilizes 95 mm rails machined to a high angular tolerance to ensure an aligned optical path and perpendicularity with the optical table. The support rail height chosen (350 - 600 mm) determines the vertical range available for experiments and microscopy components. The 7.74" throat depth, or distance from the optical path to the support rail, provides a large working space for experiments. Components attach to the body by way of either a linear dovetail on the support rail, or a circular dovetail on the epi-illumination arm (on certain models). Please see the Microscope Dovetails tab or here for further details.

Microscope Bodies Microscope Translator

 

microscope bodyClick to Enlarge
Illumination with a Cerna microscope can come from above (yellow) or below (orange). Illumination sources (green) attach to either.

Illumination

Using the Cerna microscope body, a sample can be illuminated in two directions: from above (epi-illumination, see yellow components to the right) or from below (trans-illumination, see orange components to the right).

Epi-illumination illuminates on the same side of the sample as the viewing apparatus; therefore, the light from the illumination source (green) and the light from the sample plane share a portion of the optical path. It is used in fluorescence, confocal, and reflected light microscopy. Epi-illumination modules, which direct and condition light along the optical path, are attached to the epi-illumination arm of the microscope body via a circular D1N dovetail (see the Microscope Dovetails tab or here for details). Multiple epi-illumination modules are available, as well as breadboard tops, which have regularly spaced tapped holes for custom designs.

Trans-illumination illuminates from the opposite side of the sample as the viewing apparatus. Example imaging modalities include brightfield, differential interference contrast (DIC), Dodt gradient contrast, oblique, and darkfield microscopy. Trans-illumination modules, which condition light (on certain models) and direct it along the optical path, are attached to the support rail of the microscope body via a linear dovetail (see Microscope Dovetails tab or here). Please note that certain imaging modalities will require additional optics to alter the properties of the beam; these optics may be easily incorporated in the optical path via lens tubes and cage systems. In addition, Thorlabs offers condensers, which reshape input collimated light to help create optimal Köhler illumination. These attach to a mounting arm, which holds the condenser at the throat depth, or the distance from the optical path to the support rail. The arm attaches to a focusing module, used for aligning the condenser with respect to the sample and trans-illumination module.

Epi-Illumination Body Attachments Brightfield Illumination DIC Dodt Condensers Condenser Mounting Light Sources
Epi-Illumination Modules Breadboards
& Body Attachments		 Brightfield DIC Dodt Condensers Condenser Mounting Light Sources

 

microscope bodyClick to Enlarge
Light from the sample plane is collected through an objective (blue) and viewed using trinocs or other optical ports (pink).

Sample Viewing/Recording

Once illuminated, examining a sample with a microscope requires both focusing on the sample plane (see blue components to the right) and visualizing the resulting image (see pink components).

A microscope objective collects and magnifies light from the sample plane for imaging. On the Cerna microscope, the objective is threaded onto a nosepiece, which holds the objective at the throat depth, or the distance from the optical path to the support rail of the microscope body. This nosepiece is secured to a motorized focusing module, used for focusing the objective as well as for moving it out of the way for sample handling. To ensure a light-tight path from the objective, the microscope body comes with a bellows (not pictured).

Various modules are available for sample viewing and data collection. Trinoculars have three points of vision to view the sample directly as well as with a camera. Double camera ports redirect or split the optical path among two viewing channels. Camera tubes increase or decrease the image magnification. For data collection, Thorlabs offers both cameras and photomultiplier tubes (PMTs), the latter being necessary to detect fluorescence signals for confocal microscopy. Breadboard tops provide functionality for custom-designed data collection setups. Modules are attached to the microscope body via a circular dovetail (see the Microscope Dovetails tab or here for details).

Objectives & Accessories Objective Mounting
Body Attachments
Sample Viewing Cameras PMTs Breadboards & Body Attachments

 

microscope bodyClick to Enlarge
The rigid stand (purple) pictured is one of various sample mounting options available.

Sample/Experiment Mounting

Various sample and equipment mounting options are available to take advantage of the large working space of this microscope system. Large samples and ancillary equipment can be mounted via mounting platforms, which fit around the microscope body and utilize a breadboard design with regularly spaced tapped through holes. Small samples can be mounted on rigid stands (for example, see the purple component to the right), which have holders for different methods of sample preparation and data collection, such as slides, well plates, and petri dishes. For more traditional sample mounting, slides can also be mounted directly onto the microscope body via a manual XY stage. The rigid stands can translate by way of motorized stages (sold separately), while the mounting platforms contain built-in mechanics for motorized or manual translation. Rigid stands can also be mounted on top of the mounting platforms for independent and synchronized movement of multiple instruments, if you are interested in performing experiments simultaneously during microscopy.

Translating Platforms Rigid Stands Translation Stages for Rigid Stands Motorized XY Stage Manual XY Stage
Translating Platforms Rigid Stands Translation Stages for Rigid Stands Motorized XY Stages Manual XY Stage

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For sample viewing, Thorlabs offers trinoculars, double camera ports, and camera tubes. Light from the sample plane can be collected via cameras, photomultiplier tubes (PMTs), or custom setups using breadboard tops. Click here for additional information about viewing samples with a Cerna microscope.

Product Families & Web Presentations
Sample Viewing Breadboards
& Body Attachments		 Cameras PMTs

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Microscope objectives are held in the optical path of the microscope via a nosepiece. Click here for additional information about viewing a sample with a Cerna microscope.

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Large and small experiment mounting options are available to take advantage of the large working space of this microscope. Click here for additional information about mounting a sample for microscopy.

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Thorlabs offers various light sources for epi- and trans-illumination. Please see the full web presentation of each to determine its functionality within the Cerna microscopy platform.

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Epi-illumination illuminates the sample on the same side as the viewing apparatus. Example imaging modalities include fluorescence, confocal, and reflected light microscopy. Click here for additional information on epi-illumination with Cerna.

Product Families & Web Presentations
Epi-Illumination Web Presentation Body Attachments Light Sources
Epi-Illumination Body Attachments Light Sources

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Trans-illumination illuminates from the opposite side of the sample as the viewing apparatus. Example imaging modalities include brightfield, differential interference contrast (DIC), Dodt gradient contrast, oblique, and darkfield microscopy. Click here for additional information on trans-illumination with Cerna.

Product Families & Web Presentations
Brightfield Web Presentation DIC Web Presentation Dodt Web Presentation Condensers Web Presentation Condenser Mounting Web Presentation Illumination Kits Web Presentation Other Light Sources
Brightfield DIC Dodt Condensers Condenser Mounting Illumination Kits Other Light Sources

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The microscope body provides the foundation of any Cerna microscope. The 7.74" throat depth provides a large working space for experiments. Click here for additional information about the Cerna microscope body.

Product Families & Web Presentations
Microscope Body Web Presentation Microscope Body Translator
Microscope Bodies Microscope Translator

Fixed Arms

Cerna with Custom Trans-Illumination
Click to Enlarge
User-Built Cerna System with
Trans-Illumination Using Fixed Arms
  • Optical Ports for 7.74" Throat Depth of DIY Cerna Systems
  • CSA1001: Internal SM1 (1.035"-40) Threads and Four 4-40 Taps for 30 mm Cage System
  • CSA1002: Internal SM2 (2.035"-40) Threads and Four 4-40 Taps for 60 mm Cage System
  • 95 mm Dovetail Clamp on Back Connects to Microscope Body

When installed on a Cerna microscope body, our Fixed Arms are designed to position an optical apparatus 7.74" away from the edge of the vertical rail. Designed to provide structural support for user-built optical paths, each has a 95 mm dovetail clamp on the back that can be secured anywhere along the microscope body's linear dovetail surface by tightening two 4 mm hex setscrews.

These fixed arms are offered in three versions. The CSA1001 arm has internal SM1 (1.035"-40) through threads for Ø1" lens tubes and four 4-40 through taps for 30 mm cage systems, while the CSA1002 arm has internal SM2 (2.035"-40) through threads for Ø2" lens tubes and four 4-40 through taps for 60 mm cage systems. The image to the right uses one of each to support a home-built trans-illumination apparatus.

In order to provide additional mounting configurations, the 95 mm dovetail clamp can be separated from the plate that contains the optical port by removing four 3/32" hex cap screws. This 0.50" thick plate can be attached to the 1.00" thick clamp such that the surface of the plate is in the same plane as either the top or the bottom of the clamp. This flexibility permits the fixed arm to be mounted in whatever manner makes the most efficient use of space, while keeping the dovetail clamp's securing setscrews on the same side of the microscope body. Locating dowel pins that ensure proper alignment between the plate and the dovetail are included.

For applications that would benefit from motorized translation, please consider the CSA2100 Nosepiece (sold below).

Based on your currency / country selection, your order will ship from Newton, New Jersey  
+1 Qty Docs Part Number - Universal Price Available
CSA1001 Support Documentation
CSA1001Fixed Arm, Internal SM1 Threads, 30 mm Cage Compatible
$313.81
Today
CSA1002 Support Documentation
CSA1002Fixed Arm, Internal SM2 Threads, 60 mm Cage Compatible
$326.80
Today

Manual Translation Arms

Manual Focusing Module Specifications
Travel Range 1" (25.4 mm)
Distance per Degree Turn
of Focus Knoba		 ~2 mm / 30°
Load Capacity
Stage Mounted to
Vertical Rail		 Recommended: ≤6.6 lbs (3 kg)
Maximum: 11 lbs (5 kg)
Stage Mounted to
Horizontal Rail
 Recommended: ≤22 lbs (10 kg)
Maximum: 33 lbs (15 kg)
  • The angular distance between each groove on the focus knob is 30°.
Cerna Arms
Click to Enlarge
Dimensions Comparison
Click for Details


When using the ZFM1020 module, the surface of the arm will be flush with the bottom (or top) of the module.

When using the ZFM1030 module, the bottom surface of the arm will be at the middle of the module.

  • Manually Translate Home-Designed Optical Systems Along the Microscope Body's Optical Axis
  • Manual Translation Modules
    • 1" of Travel Along Optical Axis
    • 95 mm Dovetail Clamp on Back Connects to Microscope Body
    • Include Six M4 Cap Screws for Attaching an Arm
    • Translation Stage Adjusted Using Focusing Knobs
  • Arms that Attach to a Manual Translation Module
    • CSA2100: Internal SM2 (2.035"-40) Threads and Four 4-40 Taps for 60 mm Cage System
    • CSN100: Internal M32 x 0.75 Threads and Four 4-40 Taps for 60 mm
      Cage System
    • CSA1500: Blank Arm with Alignment Mark for Custom Machining

Our manual translation modules and arms permit Ø2" lens tubes, 60 mm cage systems, optics, and custom assemblies to be mounted at the 7.74" throat depth of a DIY Cerna system and translated along the optical axis over a 1" range.

Manual Translation Modules
Our Manual Focusing Modules consist of a 95 mm dovetail clamp that connects to the microscope body, a manual translation stage, and a mounting bracket that connects to an arm. We offer two versions of these manual focusing modules to allow the user to choose whichever module makes the most efficient use of space. As shown in the drawing on the right, an arm that is mounted to the ZFM1020 Focusing Module will have one surface in the same plane as the edge of the module. This module can be secured to the microscope with the holder facing up or down. By comparison, an arm that is mounted to the ZFM1030 Focusing Module will have one surface in the plane that bisects the module, which is 1.5" away from the module's edge.

For applications requiring high-precision translation of arms along the optical axis, we recommend using the motorized translating modules below.

Arms
When installed on a manual translation module, these arms are designed to position an optical port 7.74" away from the edge of the vertical rail. The CSA2100 arm has internal SM2 (2.035"-40) through threads for Ø2" lens tubes and four 4-40 through taps for 60 mm cage systems. The CSN100 nosepiece has internal M32 x 0.75 threads and four 4-40 through taps for 60 mm cage systems. We also offer microscope thread adapters to convert M32 x 0.75 threads to other industry-standard objective threads. For alternate thread and mounting options, the CSA1500 blank arm features a center mark to align custom machining with the optical path. Custom machining can be done on your own or you can contact Tech Support to request a specific thread.

Based on your currency / country selection, your order will ship from Newton, New Jersey  
+1 Qty Docs Part Number - Universal Price Available
ZFM1020 Support Documentation
ZFM1020Manual Condenser Focusing Module with 1" Travel for Edge-Mounted Arms
$1,692.14
5-8 Days
ZFM1030 Support Documentation
ZFM1030Manual Condenser Focusing Module with 1" Travel for Middle-Mounted Arms
$1,692.14
Today
CSA2100 Support Documentation
CSA2100Arm, Internal SM2 Threads, 60 mm Cage Compatible
$153.83
Today
CSN100 Support Documentation
CSN100Nosepiece for 1 Objective, M32 x 0.75 Threads, 60 mm Cage Compatible
$109.27
5-8 Days
CSA1500 Support Documentation
CSA1500Arm, Blank with Alignment Mark
$153.83
Today

Motorized Translation Arms

Motorized Translation Stage Specificationsa
Travel Range 1" (25.4 mm)
Bidirectional Repeatability 1 µm
Backlash 1 µm
Minimum Achievable
Incremental Movement		 100 nm
Minimum Achievable
Repeatable Movement		 200 nm
Velocity (Max) 7 mm/s
Acceleration (Max) 11 mm/s2
Cable Length 6' (1.8 m)
Pin Diagram Click to View
Load Capacity
Stage Mounted to
Vertical Railb		 Recommended: ≤10 lbs (4.5 kg)
Maximum: 10 lbs (4.5 kg)
Stage Mounted to
Horizontal Rail
 Recommended: ≤33.5 lbs (15.2 kg)
Maximum: 42 lbs (19.1 kg)
Stepper Motor Specifications
Thread Screw Pitch 0.3 mm
Step Angle 1.8°
Limit Switches Hall Effect Sensors
Phase Current 0.49 A
Phase Resistance 5.1 Ω
Phase Inductance 1.5 mH
Insulation Resistance 20 MΩ
  • The ZFM2020 and ZFM2030 modules use the same motorized translation stage (Operated by MCM3001, Sold Separately).
  • This is the orientation shown in the pictures below.
Cerna Arms
Click to Enlarge
  • Translate Home-Designed Optical Systems Along the Microscope Body's Optical Axis
  • Motorized Modules (Operated by MCM3001, Sold Separately)
    • 1" of Fine Travel Along Optical Axis
    • 95 mm Dovetail Clamp on Back Connects to Microscope Body
    • Include Six M4 Cap Screws for Attaching an Arm
  • Arms that Attach to a Motorized Module
    • CSA2100: Internal SM2 (2.035"-40) Threads and Four 4-40 Taps for 60 mm Cage System
    • CSN100: Internal M32 x 0.75 Threads and Four 4-40 Taps for 60 mm
      Cage System
    • CSA1500: Blank Arm with Alignment Mark for Custom Machining

Our motorized modules and arms permit Ø2" lens tubes, 60 mm cage systems, optics, and custom assemblies to be mounted at the 7.74" throat depth of a DIY Cerna system and translated along the optical axis over a 1" range.

Motorized Modules
Our Motorized Focusing Modules consist of a 95 mm dovetail clamp that connects to the microscope body, a motorized translation stage, and a mounting bracket that connects to an arm. We offer two versions of these stepper motor modules in order to allow the user to mount the arm in whatever manner makes the most efficient use of space. As shown in the drawing below, an arm that is mounted to the ZFM2020 Motorized Module will have one surface in the same plane as the edge of the module. Since this module can be secured to the microscope body in either of two orientations, both of which are shown in the image below, the arm can be positioned at the top or the bottom. In comparison, an arm that is mounted to the ZFM2030 Motorized Module will have one surface in the plane that bisects the module, which is 1.5" away from the module's edge.

The ZFM2020 and ZFM2030 modules use the same motorized translation stage; its specifications are given to the table to the right. Both are operated by the MCM3001 3-Axis Controller (sold separately, see below).

Arms
When installed on a motorized module, these arms are designed to position an optical port 7.74" away from the edge of the vertical rail. The CSA2100 arm has internal SM2 (2.035"-40) through threads for Ø2" lens tubes and four 4-40 through taps for 60 mm cage systems. The CSN100 nosepiece has internal M32 x 0.75 threads and four 4-40 through taps for 60 mm cage systems. We also offer microscope thread adapters to convert M32 x 0.75 threads to other industry-standard objective threads. For alternate thread and mounting options, the CSA1500 blank arm features a center mark to align custom machining with the optical path. Custom machining can be done on your own or you can contact Tech Support to request a specific thread.

SM2 Attachment
Click to Enlarge
Our motorized modules attach to our arms using six M4 cap screws.
SM2 Cerna Attachment
Click to Enlarge
The motorized module positions the optical port of the arm at a 7.74" throat depth. Here, the ZFM2020 module and CSA2100 arm are shown.
ZFM2020 in Two Orientations
Click for Details
The ZFM2020 module has two possible orientations, creating space along the optical path for mounted optomechanical assemblies.
Dimensions Comparison
Click for Details

When using the ZFM2020 module, the surface of the arm will be flush with the bottom (or top) of the module.

When using the ZFM2030 module, the surface of the arm will be at the middle of the module.

Based on your currency / country selection, your order will ship from Newton, New Jersey  
+1 Qty Docs Part Number - Universal Price Available
ZFM2020 Support Documentation
ZFM2020Motorized Module with 1" Travel for Edge-Mounted Arms
$2,010.41
Today
ZFM2030 Support Documentation
ZFM2030Motorized Module with 1" Travel for Middle-Mounted Arms
$2,010.41
Today
CSA2100 Support Documentation
CSA2100Arm, Internal SM2 Threads, 60 mm Cage Compatible
$153.83
Today
CSN100 Support Documentation
CSN100Nosepiece for 1 Objective, M32 x 0.75 Threads, 60 mm Cage Compatible
$109.27
5-8 Days
CSA1500 Support Documentation
CSA1500Arm, Blank with Alignment Mark
$153.83
Today

Motion Controller for Cerna Components with 1" Travel Range

Motorized Rigid Stand
Click to Enlarge
MCM3001 Being Used to Control Both Axes of the PLS-XY and a ZFM2020 Focusing Module

Compatible Stages
Motorized Focusing Modules
Translation Stages for Rigid Stands
Controller Specifications
Compatible Motor Specifications
  • Designed for Cerna Components with 1" Motorized Travel
  • Knobs Provide Hand-Operated Control for up to Three Axes
  • Each Axis can be Individually Disabled to Prevent
    Unintended Movements or to Retain a Position
  • Adjust Translation Speed via Top-Located Knob

The MCM3001 3-Axis Controller consists of a hand-operated knob box and a separate controller, as shown in the photo to the right. Each side face of the knob box includes a rotating knob and a push-button switch that are dedicated to a single axis. The push-button switch enables and disables the axis, and is lit in green when the axis is enabled. Disabling the axis lets the user preserve a position or prevent accidental movements. A smaller knob on the top face adjusts the amount of translation per rotation of the knob (see the Controller Specifications table for details).

Since each MCM3001 controller has three channels, you only need to purchase enough channels for each of the modules you intend to drive. For example, a Cerna microscope equipped with a ZFM2020 Motorized Focusing Module (which has one axis) and a PLS-XY Translation Stage (two axes) would only require one MCM3001 controller.

The MCM3001 is compatible with motorized Cerna components that have a travel range of 1", such as our Motorized Focusing Modules and Translation Stages for Rigid Stands; see the Compatible Motor Specifications table for use with alternate motorized products. For components with a 2" travel range, such as our Translating Platforms, the MCM3002 controller should be used instead. If you would like a controller configured to drive more than one type of stage, please contact Tech Support.

SDK and LabVIEW examples are also available by contacting Tech Support.

Based on your currency / country selection, your order will ship from Newton, New Jersey  
+1 Qty Docs Part Number - Universal Price Available
MCM3001 Support Documentation
MCM3001Three-Channel Controller and Knob Box for 1" Cerna Travel Stages
$3,368.64
Today

Mounting Platforms

XT95P12 Clamping Mechanism
Click to Enlarge
XT95P12 Rail Plate with T-Nuts Inserted in 95 mm Rail Channels
XT95P11 on Rail Side
Click to Enlarge
XT95RC1 Drop-On Rail Carriage Attached to a 95 mm Optical Rail
  • Provide Arrays of Mounting Holes for Creating DIY Microscope Modules
  • Positioned by Sliding Along the Microscope Body Rail
  • Drop-On Rail Carriages: Insert and Remove Anywhere Along the Vertical Rail
  • XT95P12(/M) Rail Plate: Insert at Rail End and Slide Along Rail
Item # Length Number of
Mounting Holes		 Mounting Hole Type Locking Screw Type
XT95RC1 1.00" 7 1/4"-20 Tapped 1/4"-20
(3/16" Hex)		 Drop-On
Rail Carriage
XT95RC2 2.00" 21
XT95RC3 3.00" 35
XT95RC4 4.00" 49
XT95RC1/M 25.0 mm 7 M6 x 1.0 Tapped M6
(5.0 mm Hex)
XT95RC2/M 50.0 mm 21
XT95RC3/M 75.0 mm 35
XT95RC4/M 100.0 mm 49
XT95P12 3.15" 17 Threaded,
4 Counterbore		 1/4"-20 Tapped,
1/4" Counterbore		 Four 1/4"-20
(3/16" Hex)		 Rail Plate
XT95P12/M 81.3 mm M6 x 1.0 Tapped,
M6 Counterbore		 Four M6
(5.0 mm Hex)

These mounting platforms can be used to mount home-built microscope modules to the 95 mm dovetail on a Cerna microscope body. Each platform includes a matrix of mounting holes for compatibility with our extensive selection of optomechanics.

The Drop-On Rail Carriages can clamp to the side of a 95 mm rail by tightening the side-located 1/4"-20 (M6) locking screws. When the locking screw is loosened, the assisted opening mechanism pushes the hinged clamping arm out and provides ample clearance for the dovetail of a 95 mm rail. This allows the carriage to be inserted and removed anywhere along an exposed rail side. Furthermore, while the carriage is clamped onto a rail, the locking screw can be loosened slightly, allowing the carriage to slide along the rail edge and be repositioned in another location.

In comparison, the XT95P12(/M) Rail Plate includes a closely packed array of seventeen 1/4"-20 (M6) tapped holes and four 1/4" (M6) counterbores. Tightening the four included 1/4"-20 (M6) cap screws locks the rail plate in place. As shown in the image to the far right, these cap screws are attached to two T-Nuts that fit into the channels on the 95 mm dovetail. Because accessing these channels on a DIY Cerna microscope body requires the end of the rail to be available, the top plate of the body must be detached in order to install and remove this plate. We therefore only recommend this rail plate for microscope bodies without epi-illumination arms. It is difficult to reinstall and realign an epi-illumination arm that has an optical setup on it.

If using a rail carriage and rail plate in the same setup, please note that the distance between the mounting surface and the top of the rail will not be the same, as shown in this photo.

The Drop-On Rail Carriages can be attached anywhere along the 95 mm dovetail of the Cerna microscope body.
The XT95P12 Rail Plate is inserted through the rail channels, requiring the top plate of the microscope body to be removed. It is only recommended for microscope bodies without epi-illumination arms.
Based on your currency / country selection, your order will ship from Newton, New Jersey  
+1 Qty Docs Part Number - Imperial Price Available
XT95RC1 Support Documentation
XT95RC1Drop-On Rail Carriage for 95 mm Rails, 1.00" Long, 1/4"-20 Tapped Holes
$78.51
Today
XT95RC2 Support Documentation
XT95RC2Drop-On Rail Carriage for 95 mm Rails, 2.00" Long, 1/4"-20 Tapped Holes
$92.30
Today
XT95RC3 Support Documentation
XT95RC3Drop-On Rail Carriage for 95 mm Rails, 3.00" Long, 1/4"-20 Tapped Holes
$111.39
Today
XT95RC4 Support Documentation
XT95RC4Drop-On Rail Carriage for 95 mm Rails, 4.00" Long, 1/4"-20 Tapped Holes
$130.49
Today
XT95P12 Support Documentation
XT95P12Rail Plate for 95 mm Rails, 3.15" Long, 1/4"-20 Tapped Holes
$48.97
Today
+1 Qty Docs Part Number - Metric Price Available
XT95RC1/M Support Documentation
XT95RC1/MDrop-On Rail Carriage for 95 mm Rails, 25.0 mm Long, M6 Tapped Holes
$78.51
Today
XT95RC2/M Support Documentation
XT95RC2/MDrop-On Rail Carriage for 95 mm Rails, 50.0 mm Long, M6 Tapped Holes
$92.30
Today
XT95RC3/M Support Documentation
XT95RC3/MDrop-On Rail Carriage for 95 mm Rails, 75.0 mm Long, M6 Tapped Holes
$111.39
Today
XT95RC4/M Support Documentation
XT95RC4/MDrop-On Rail Carriage for 95 mm Rails, 100.0 mm Long, M6 Tapped Holes
$130.49
Lead Time
XT95P12/M Support Documentation
XT95P12/MRail Plate for 95 mm Rails, 81.3 mm Long, M6 Tapped Holes
$48.97
Today

Breadboard Tops for Microscope Bodies


Click for Details
CSA3000 Used to Mount a Custom Epi-Illuminator and Widefield Viewing Apparatus

Click for Details
CSA3010 Used to Mount a Custom Epi-Illuminator and Widefield Viewing Apparatus
  • Male D1N Dovetail on Bottom for Attachment to DIY Cerna Microscope Bodies
  • Available in Two Sizes in Imperial and Metric Versions:
    • Imperial: 14.00" x 11.00" or 18.00" x 4.60"
    • Metric: 350.0 mm x 275.0 mm or 450.0 mm x 116.8 mm
  • 1/4"-20 or M6 x 1.0 Mounting Holes
Male D1N Dovetail
Click to Enlarge
Each breadboard has a male D1N dovetail on the bottom.

These black-anodized aluminum breadboard tops support user-designed widefield viewing apparatuses, epi-illumination pathways, and laser scanning pathways on top of upright Cerna microscopes. Each contains a Ø1.5" (Ø38.1 mm) through hole that is centered on a male D1N dovetail. This dovetail allows the breadboard to be connected directly to the epi-illumination arm of the microscope body, and it can also be used to stack the breadboard on top of an epi-illumination module. Additional details on the dovetail are available in the Microscope Dovetails tab.

The breadboards are available in two sizes. The larger version [Item # CSA3000(/M)] provides additional work surface, but protrudes past the sides of the epi-illumination arm, which may restrict approach angles around the objective for micromanipulators. The smaller version [Item # CSA3010(/M)] does not restrict approach angles and also has eight 4-40 taps around the Ø1.5" through hole for 30 mm and 60 mm cage systems.

In configurations where the breadboard is mounted directly on top of the epi-illumination arm, four M4 counterbores can be used to provide additional mounting stability.

Item # CSA3000 CSA3000/M CSA3010 CSA3010/M
Dimensions (L x W) 14.00" x 11.00" 350.0 mm x 275.0 mm 18.00" x 4.60" 450.0 mm x 116.8 mm
Breadboard Thickness 1/2" 12.7 mm 1/2" 12.7 mm
Hole Size and Spacing 1/4"-20 Tapped Holes
on 1" Centers		 M6 x 1.0 Tapped Holes
on 25 mm Centers		 1/4"-20 Tapped Holes
on 1" Centers		 M6 x 1.0 Tapped Holes
on 25 mm Centers
Number of Tapped Holes
 154 154 87 89
Cage System Compatibility - Four 4-40 Taps for 30 mm Cage Systems
Four 4-40 Taps for 60 mm Cage Systems
Click for Mechanical Drawing
Dovetail Male D1N
Material Matte Black Anodized Aluminum
Based on your currency / country selection, your order will ship from Newton, New Jersey  
+1 Qty Docs Part Number - Imperial Price Available
CSA3000 Support Documentation
CSA3000Breadboard Top, 14.00" x 11.00", 1/4"-20 Taps, Male D1N Dovetail
$737.33
Today
CSA3010 Support Documentation
CSA3010Breadboard Top, 18.00" x 4.60", 1/4"-20 Taps, Male D1N Dovetail
$896.46
Today
+1 Qty Docs Part Number - Metric Price Available
CSA3000/M Support Documentation
CSA3000/MBreadboard Top, 350.0 mm x 275.0 mm, M6 x 1.0 Taps, Male D1N Dovetail
$737.33
Today
CSA3010/M Support Documentation
CSA3010/MBreadboard Top, 450.0 mm x 116.8 mm, M6 x 1.0 Taps, Male D1N Dovetail
$896.46
Today

Breadboard Top with Two-Position Slider

Two Independent Epi-Illumination Paths
Click to Enlarge
View Imperial Product List
Item #QtyDescription
Items on Microscope Body
CEA14001Cerna Microscope Body with Epi-Illumination Arm, 400 mm Rail
ZFM20201Motorized Module with 1" Travel for Edge-Mounted Arms
CSA12001Mounting Arm for CSN1201 and CSN1202 Nosepieces
CSN12021Nosepiece for 2 Objectives, M25 x 0.75 Threads
MCM30011Three-Channel Controller and Knob Box for 1" Cerna Travel Stages
Items on Bottom Optical Path
OPX24001Breadboard Top with Slider, 1/4"-20 Taps, Male & Female D1N Dovetails
PFR14-P02135 mm x 52 mm Protected Silver Mirror
LCP01360 mm Cage Plate, SM2 Threads, 0.5" Thick, 8-32 Tap (Two SM2RR Retaining Rings Included)
LA14012N-BK7 Plano-Convex Lens, Ø2", f = 60 mm, Uncoated
LA13011N-BK7 Plano-Convex Lens, Ø2", f = 250 mm, Uncoated
LCP50S160 mm Cage System Iris Diaphragm (Ø2 - Ø50 mm)
LCFW5160 mm Cage Filter Wheel for Five Ø2" (Ø50 mm) Filters
NE2R03B1Unmounted Ø2" Absorptive ND Filter, Optical Density: 0.3
NE2R13B1Unmounted Ø2" Absorptive ND Filter, Optical Density: 1.3
FGT202001Ø2" Temperature-Balancing Filter, -160 mireds
SLSLLG11Liquid Light Guide Adapter for Broadband Light Sources, 185 nm - 2.1 µm
TRP0572Ø0.47" Pedestal Pillar Post, 8-32 Setscrew, 1/4"-20 Tap, L = 0.568"
MSC22Clamping Fork for 1/4"-20 (M6) Cap Screw
ER8-P41Cage Assembly Rod, 8" Long, Ø6 mm, 4 Pack
ER4-P41Cage Assembly Rod, 4" Long, Ø6 mm, 4 Pack
Items on Top Optical Path
WFA20021Epi-Illuminator Module for 1 Cube, Male & Female D1N Dovetails
TLV-U-MF21Microscopy Cube Assembly for Olympus AX, BX2, and IX2 Microscopes
DMLP490R125 mm x 36 mm Longpass Dichroic Mirror, 490 nm Cut-On
CP02F2SM1-Threaded 30 mm Flexure Cage Plate, 0.35" Thick, 2 Retaining Rings, Imperial
LA1172-A1N-BK7 Plano-Convex Lens, Ø1", f = 400 mm, AR Coating: 350 - 700 nm
LA1461-A1N-BK7 Plano-Convex Lens, Ø1", f = 250 mm, AR Coating: 350 - 700 nm
CP36130 mm Cage Plate, Ø1.2" Double Bore for SM1 and C-Mount Lens Tubes
M455L41455 nm, 1150 mW (Min) Mounted LED, 1000 mA
LAURE11Cerna Trinoculars with 10X Eyepieces, Upright Image, IR Filter
View Metric Product List
Item #QtyDescription
Items on Microscope Body
CEA14001Cerna Microscope Body with Epi-Illumination Arm, 400 mm Rail
ZFM20201Motorized Module with 1" Travel for Edge-Mounted Arms
CSA12001Mounting Arm for CSN1201 and CSN1202 Nosepieces
CSN12021Nosepiece for 2 Objectives, M25 x 0.75 Threads
MCM30011Three-Channel Controller and Knob Box for 1" Cerna Travel Stages
Items on Bottom Optical Path
OPX2400/M1Breadboard Top with Slider, M6 x 1.0 Taps, Male & Female D1N Dovetails
PFR14-P02135 mm x 52 mm Protected Silver Mirror
LCP01/M360 mm Cage Plate, SM2 Threads, 0.5" Thick, M4 Tap (Two SM2RR Retaining Rings Included)
LA14012N-BK7 Plano-Convex Lens, Ø2", f = 60 mm, Uncoated
LA13011N-BK7 Plano-Convex Lens, Ø2", f = 250 mm, Uncoated
LCP50S160 mm Cage System Iris Diaphragm (Ø2 - Ø50 mm)
LCFW5160 mm Cage Filter Wheel for Five Ø2" (Ø50 mm) Filters
NE2R03B1Unmounted Ø2" Absorptive ND Filter, Optical Density: 0.3
NE2R13B1Unmounted Ø2" Absorptive ND Filter, Optical Density: 1.3
FGT202001Ø2" Temperature-Balancing Filter, -160 mireds
SLSLLG11Liquid Light Guide Adapter for Broadband Light Sources, 185 nm - 2.1 µm
TRP14/M2Ø12 mm Pedestal Pillar Post, M4 Setscrew, M6 Tap, L = 14.4 mm
MSC22Clamping Fork for 1/4"-20 (M6) Cap Screw
ER8-P41Cage Assembly Rod, 8" Long, Ø6 mm, 4 Pack
ER4-P41Cage Assembly Rod, 4" Long, Ø6 mm, 4 Pack
Items on Top Optical Path
WFA20021Epi-Illuminator Module for 1 Cube, Male & Female D1N Dovetails
TLV-U-MF21Microscopy Cube Assembly for Olympus AX, BX2, and IX2 Microscopes
DMLP490R125 mm x 36 mm Longpass Dichroic Mirror, 490 nm Cut-On
CP02F/M2SM1-Threaded 30 mm Flexure Cage Plate, 0.35" Thick, 2 Retaining Rings, Metric
LA1172-A1N-BK7 Plano-Convex Lens, Ø1", f = 400 mm, AR Coating: 350 - 700 nm
LA1461-A1N-BK7 Plano-Convex Lens, Ø1", f = 250 mm, AR Coating: 350 - 700 nm
CP36130 mm Cage Plate, Ø1.2" Double Bore for SM1 and C-Mount Lens Tubes
M455L41455 nm, 1150 mW (Min) Mounted LED, 1000 mA
LAURE11Cerna Trinoculars with 10X Eyepieces, Upright Image, IR Filter
Here, a white-light illumination path has been connected to the OPX2400 using our 60 mm cage system, and a GFP fluorescence path has been mounted on top of the OPX2400 via our WFA2002 epi-illuminator module.
  • Two-Position Slider to Combine or Switch Between DIY Optical Paths
  • Slider has Internal SM2 Threads and Holds One 35 mm x 52 mm x 3 mm Optic
  • Back Port has Internal SM2 Threads and Four 4-40 Taps for Our 60 mm Cage System
  • Imperial and Metric Versions
    • OPX2400: 10.16" x 3.94" Breadboard with Double-Density 1/4"-20 Tapped Holes
    • OPX2400/M: 258 mm x 100 mm Breadboard with Double-Density M6 x 1.0 Tapped Holes
  • Stackable Design with Female and Male D1N Dovetails on Top and Bottom, Respectively
Two Independent Epi-Illumination Paths
Click to Enlarge
Slider Located Above Objective
Two Independent Epi-Illumination Paths
Click to Enlarge
Slider Not in Optical Path with Objective

The lid of the slider housing is opened by removing four cap screws with a 3 mm balldriver. The slider and the slider housing are internally SM2-threaded. Two stainless steel tracks and detents provide repeatable positioning.

The OPX2400(/M) Breadboard Top with Two-Position Slider adds a manually operated optic slider to the epi-illumination arm of a Cerna microscope body. By mounting a dichroic, beamsplitter, or mirror into the slider, users may combine or switch between widefield viewing, epi-illumination, and/or laser scanning pathways.

The optic slider has a clear aperture of Ø1.65" (Ø41.9 mm) and uses a leaf spring to retain a rectangular optic (minimum size: 34.9 mm x 51.9 mm x 2.8 mm; maximum size: 35.0 mm x 52.0 mm x 3.2 mm); the large aperture and optic size allow the entire aperture of the scan lenses above to be utilized. It has internal SM2 (2.035"-40) threads that face the back of the stationary housing, allowing a tube lens to be installed at a fixed distance from the dichroic. The back of the housing also has internal SM2 threads, as well as four 4-40 taps spaced for our 60 mm cage system.

In addition, a breadboard with sixty-eight 1/4"-20 (M6 x 1.0) through-tapped holes in a double-density hole pattern is included to support a home-built optical path. More 1/4"-20 (M6 x 1.0) tapped holes (sixteen on the imperial version and eighteen on the metric version) are located on the sides of the breadboard. Measured from the top of the breadboard, the beam height is 50.0 mm. Thorlabs manufactures Ø12 mm pedestal posts that center many of our 30 mm and 60 mm cage plates at this beam height, as illustrated in this photo, which provide structural support for large or heavy setups.

Thorlabs offers a 750 nm shortpass dichroic (Item # DMSP750B) and a protected silver mirror (Item # PFR14-P02) as stocked items. Beamsplitters and dichroics at additional cutoff wavelengths are available by contacting Tech Support. Once the optic is mounted, a 5/64" (2 mm) hex balldriver can be used to fine tune the optic slider's pitch and yaw adjusters. The slider may be locked in either position by tightening the included locking screw with a 3/32" balldriver. In the photo to the upper right, the locking screw is installed in the forward position.

In laser scanning Cerna systems, we recommend attaching the tube lens using the internal SM2 threads on the slider, since this will maximize the distance available along the throat depth to mount the objective and, if desired, non-descanned detectors.

Based on your currency / country selection, your order will ship from Newton, New Jersey  
+1 Qty Docs Part Number - Imperial Price Available
OPX2400 Support Documentation
OPX2400Breadboard Top with Slider, 1/4"-20 Taps, Male & Female D1N Dovetails
$4,660.68
Today
+1 Qty Docs Part Number - Universal Price Available
DMSP750B Support Documentation
DMSP750B35 mm x 52 mm Shortpass Dichroic Mirror, 750 nm Cutoff
$1,435.52
Today
PFR14-P02 Support Documentation
PFR14-P0235 mm x 52 mm Protected Silver Mirror
$530.45
Today
+1 Qty Docs Part Number - Metric Price Available
OPX2400/M Support Documentation
OPX2400/MBreadboard Top with Slider, M6 x 1.0 Taps, Male & Female D1N Dovetails
$4,660.68
Today

Epi-Illuminator Module for One Filter Cube


Click to Enlarge
The front of the module has a magnetic door cover that holds a filter cube.


Click to Enlarge
The photo above shows a home-built epi-illumination path mounted on the WFA2002 via a 30 mm cage system.

Click to Enlarge
The back of the WFA2002 module has internal SM1 threads and four 4-40 taps for our 30 mm cage system.
  • Internal SM1 (1.035"-40) Threads, Four 4-40 Taps for 30 mm
    Cage Systems, and Female and Male D1N Dovetails
  • Magnetic Door Cover Holds Filter Cube in Optical Path
This animation shows the installation of a loaded filter cube into the WFA2002 Epi-Illuminator Module.

The WFA2002 Epi-Illuminator Module holds one filter cube in the epi-illumination pathway of a DIY Cerna system. With a female D1N dovetail on top and a male D1N dovetail on the bottom, it is designed to mate with the epi-illumination arm of a Cerna microscope body, as well as with other epi-illuminator modules. Additional details on dovetails are available in the Microscope Dovetails tab.

As shown in the image to the right, its optical input port has internal SM1 (1.035"-40) threads and four 4-40 taps for our 30 mm cage system. These mechanical interfaces enable Thorlabs' extensive selection of SM1 and 30 mm cage components to be used to build custom epi-illumination paths.

The module includes a magnetically secured cover that can be connected to an MDFM-MF2 Filter Cube, manufactured by Olympus. The animation to the left shows the filter cube installation procedure. Extra covers, which the user can attach to the filter cubes to speed up filter cube exchange, are sold as Item # WFA2001C. These filter cubes can be used to hold fluorescence filter sets, beamsplitters with crossed polarizers, or mirrors.

We also offer the WFA2001 Epi-Illuminator Module, which ships ready to accept an uncollimated light source with a Ø3 mm core liquid light guide or an SM1-coupled output.

Based on your currency / country selection, your order will ship from Newton, New Jersey  
+1 Qty Docs Part Number - Universal Price Available
WFA2002 Support Documentation
WFA2002Epi-Illuminator Module for 1 Cube, Male & Female D1N Dovetails
$419.06
Today
WFA2001C Support Documentation
WFA2001CExtra Filter Cube Cover for WFA2001 and WFA2002 Epi-Illuminator Modules
$188.28
5-8 Days
MDFM-MF2 Support Documentation
MDFM-MF2OEM Microscopy Cube Assembly for Olympus AX, BX2, IX2, and Thorlabs Cerna Microscopes with WFA2001 or WFA2002 Epi-Illuminator Module
$523.74
Today

Trinocular Eyepiece Adapter

Item # SM2N2
Microscope Connection Ø1.18" Eyepiece Tube
(Alignment Slot, 5 Places)
SM Threading
 Internal SM1 (1.035"-40)
External SM2 (2.035"-40)
Cage Compatibility 30 mm Cage System
(4-40 Tap, One Side,
4 Places)
Clear Aperture Ø0.90" (22.9 mm)
Adapter Profile
(Click for Drawing)

Click to Enlarge
Two SM2N2 adapters attached to the trinocular eyepieces.
  • Replace Trinocular Eyepiece for DIY Construction
  • Compatible with SM1, SM2, and 30 mm Cage Construction Systems

The SM2N2 Eyepiece Adapter allows custom-built optical detection systems to attach to either eyepiece on the trinoculars of a Cerna Microscope. This adapter replaces the lens element on the eyepiece that sets the image plane at the the back of the eyes (see image to the right). Five alignment slots ensure the adapter fits snugly inside the eyepiece without rotation; because of the drop-in nature of this adapter, take care the attached system does not overbalance the 40 g eyepiece adapter when it is inside the trinoculars.

This adapter features internal SM1 (1.035"-40) threading for Ø1" lens tubes; two SM1RR retaining rings are included to secure an optic inside the adapter. The adapter also has external SM2 (2.035"-40) threading for Ø2" lens tubes. The face with the item # engraving has 4-40 tapped holes for 30 mm cage systems.

Based on your currency / country selection, your order will ship from Newton, New Jersey  
+1 Qty Docs Part Number - Universal Price Available
SM2N2 Support Documentation
SM2N2Nikon Eclipse or Cerna Microscope Eyepiece Adapter, Internal SM1 and External SM2 Threads, 30 mm Cage Compatibility
$143.22
Today

Dovetail Adapters

  • Extend Versatility of Our Lens Tube and Cage Construction Systems to DIY Cerna Systems
  • Compatible with DIY Cerna Modules that Have D1N, D2N, D2NB, D3N, or D3T Dovetails

The items shown here represent our complete selection of dovetail adapters for Thorlabs' Cerna microscopy platform. They integrate the Cerna platform with our SM1 (1.035"-40) lens tube, SM30 (M30.5 x 0.5) lens tube, SM2 (2.035"-40) lens tube, 30 mm cage, and 60 mm cage construction systems, making them ideal for creating custom widefield viewing, epi-illumination, trans-illumination, and condenser mounting apparatuses. Additionally, we offer the LCPN3 trinocular port adapter, designed to allow Olympus trinoculars that have a male D5Y dovetail to be used with DIY Cerna systems. See the images and table below for application ideas and the adapters' mechanical features.

The D1N, D2N, and D2NB dovetails are primarily used by our widefield viewing and epi-illumination accessories, while the D3N is Thorlabs' designation for the dovetail used by the majority of Nikon condensers for upright microscopes. The D3T dovetail is compatible with our CSE2100 epi-illuminator module. Lastly, the D5Y is Thorlabs' designation for the dovetail used by the majority of Olympus widefield viewing modules.

Application Ideas


Click to Enlarge
Here, our WFA4111 D1N Adapter is being used to support an SM2 lens tube that contains user-selected optics for forming an image on a Scientific Camera.
Cerna Camera Port Adapter
Click for Details
In this photo, the CSA1003 D1N Adapter is connecting a 60 mm cage system to a WFA2002 Epi-Illuminator Module.
Cerna Camera Port Adapter
Click to Enlarge
In this setup, the SM2A56 D2N Adapter is connecting a Solis® high-powered LED to the back of a CSE2100 epi-illuminator module.
Cerna Custome Condenser
Click to Enlarge
Our CSA2001 D3N Adapter is used by our CSA2000 Condenser Arm to hold a condenser to our CXY2 Translation Mount. This mount provides orthogonal XY adjusters for the condenser.
Item # Dovetaila Threading Cage Compatibility Clear Aperture Built-In
Tube Lens		 Adapter Profile
(Click for Drawing)
WFA4111b Male D1N Internal M38 x 0.5c
External SM2		 Noned Ø1.47" (37.0 mm) -
WFA4110b Male D1N External SM2 Noned - TTL200
LCPN2 Male D1N Internal SM30e 30 mm Cage System
(4-40 Tapf, 4 Places)
60 mm Cage System
(Ø6 mm Bore, 4 Places)		 Ø1.10" (27.9 mm) -
LCPN3 Male D1N
Female D5Y		 Internal SM30e 60 mm Cage System
(Ø6 mm Bore, 4 Places)		 Ø1.10" (27.9 mm) -
CSA1003 Female D1N Noned 60 mm Cage System
(Ø6 mm Bore, 4 Places)		 Ø1.50" (38.1 mm) -
SM1A58 Male D2N
Male D2NB		 Internal SM1g
External SM2		 30 mm Cage System
(4-40 Tap, 4 Places)		 Ø1.008" (25.6 mm) -
LCPN1 Male D3N Internal SM30e 30 mm Cage System
(4-40 Tap, 4 Places)
60 mm Cage System
(Ø6 mm Bore, 4 Places)		 Ø1.10" (27.9 mm) -
CSA2001 Female D3N External SM2 Noned Ø1.58" (40.0 mm) -
SM2A56 Male D3T External SM2 Noned Ø1.40" (35.6 mm) -
  • Additional information on dovetails is available in the Microscope Dovetails tab.
  • Item #'s WFA4111 and WFA4110 have identical mechanical profiles, but Item # WFA4110 has a permanently installed TTL200 Tube Lens, which can be used to construct a custom camera tube.
  • This internal M38 x 0.5 threading is compatible with our SM38RR retaining rings.
  • An SM2-threaded cage plate can be used to convert between SM2 lens tubes and 60 mm cage systems.
  • This internal SM30 threading is compatible with our SM30RR retaining rings. Two SM30RR retaining rings are included.
  • These tapped holes are on the side opposite the dovetail only.
  • This internal SM1 threading is not deep enough for mounting optics.
Based on your currency / country selection, your order will ship from Newton, New Jersey  
+1 Qty Docs Part Number - Universal Price Available
WFA4111 Support Documentation
WFA4111Adapter with Male D1N Dovetail, External SM2 Threads, and Internal M38 x 0.5 Threads
$318.15
Today
WFA4110 Support Documentation
WFA4110Adapter with Male D1N Dovetail, External SM2 Threads, and TTL200 Tube Lens
$618.97
Today
LCPN2 Support Documentation
LCPN2Nikon Eclipse or Cerna Microscope Trinocular Adapter, Male D1N Dovetail, Internal SM30 Threads, 30 and 60 mm Cage Compatibility
$111.39
Today
LCPN3 Support Documentation
LCPN3Customer Inspired! Nikon Eclipse or Cerna Microscope Trinocular Adapter, Male D1N Dovetail, Female D5Y Dovetail, Internal SM30 Threads, 60 mm Cage Compatibility
$73.34
5-8 Days
CSA1003 Support Documentation
CSA1003Adapter with Female D1N Dovetail and Bores for 60 mm Cage System
$264.04
Today
SM1A58 Support Documentation
SM1A58Upright Nikon Eclipse and Thorlabs Cerna Microscope Camera Port Adapter, Internal SM1 Threads, External SM2 Threads, 30 mm Cage Compatible
$81.70
Today
LCPN1 Support Documentation
LCPN1Nikon Eclipse or Cerna Microscope Condenser Adapter, Male D3N Dovetail, Internal SM30 Threads, 30 and 60 mm Cage Compatibility
$106.09
Today
CSA2001 Support Documentation
CSA2001Adapter with Female D3N Dovetail and External SM2 Threads
$315.98
Today
SM2A56 Support Documentation
SM2A56Adapter with Male D3T Dovetail and External SM2 Threads
$106.09
Today
Additional DIY Cerna Components: Imaging for Home Builders
  • Microscope Bodies

  • Body Attachments & Extensions

  • Sample Holders

  • Objective Mounting

  • Objectives

  • Condenser Mounting

  • Condensers

  • Trans-Illumination: Brightfield, Oblique, & Others

  • Trans-Illumination: DIC

  • Trans-Illumination: Dodt Gradient Contrast

  • Epi-Illumination

  • Scientific Cameras

  • Microscopy Filters

  • Illumination Sources

  • Widefield Viewing

  • Laser Scanning

  • Tunable Ti:Sapphire Laser for Multiphoton Imaging

  • Detectors

  • Pre-Configured Cerna Microscopes

  • High-Speed Optical Modulator

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