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Four-Channel, Cerna®-Based Confocal Systems
Each Four-Channel Confocal System includes a computer, DAQ, and ThorImage®LS Data Acquisition Software. The optical table and the rack system are sold separately.
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Stitched Image of Cell Layers in Mouse Retina
Sample Courtesy of Robert Fariss, Biological Imaging Core, NIH, Bethesda, Maryland
Thorlabs' Confocal Microscopy System
Thorlabs' Upright Confocal Microscopy Systems are complete, fully equipped microscopes available with galvo-galvo or galvo-resonant scan heads to support a variety of imaging applications. By eliminating signals that originate from outside the focal plane, confocal microscopy provides the ability to acquire high-resolution, optically sectioned images from within a thick sample or to reduce background fluorescence from thin cultures. Each system is tailored to meet individual imaging requirements; contact us using the link in the green box to the upper right for additional information and to receive a quote.
For applications that require rapid imaging of live systems, our galvo-resonant scan head is capable of capturing up to 400 fps. Alternatively, microscopes with galvo-galvo scanners are ideal for experiments involving photo-uncaging. Select one of ten pre-configured laser sources with up to four excitation wavelengths. A motorized pinhole wheel with 16 round pinholes provides true diffraction-limited imaging while allowing the user to optimize the pinhole size for their objective. Multi-spectral imaging is enabled by a two- or four-channel detection module with an included filter set that, together with the laser source, has been optimized for the excitation and emission wavelengths of popular fluorophores. Choose from standard multialkali photomultiplier tubes (PMTs) for samples with a large dynamic range or high-sensitivity GaAsP PMTs to image weakly fluorescent samples.
Each microscope includes a PC with DAQ card and the ThorImageLS data acquisition software. ThorImageLS was developed in conjunction with our laser scanning microscopy systems to provide a seamless, logical, intuitive program for acquiring and analyzing images. This open-source software package enables synchronization of external hardware and events, multi-dimensional data acquisition and display, region-of-interest scanning, and multi-user operation. All images are saved in the standard TIFF image format so that they can be viewed using software packages such as ImageJ/Fiji. See the ThorImageLS tab for additonal information on ThorImageLS features. Upon the purchase of a confocal system, Thorlabs provides lifetime support for the ThorImageLS package.
To explore the full range of possible system configurations, including laser source and emission filter options, see the Confocal System tab. For system specifications, please see the Specs tab.
Thorlabs also offers pre-configured, upgradeable single-channel confocal systems; see the complete web presentation for details.
Thorlabs' Confocal Microscopy System
Thorlabs’ Four-Channel Confocal Laser Scanning Microscopy Systems consist of a Cerna®-based microscope with a galvo-galvo or galvo-resonant scanner, monolithic laser source, multi-channel PMT detection module control electronics, pinhole wheel, and all fibers and cables needed to interconnect the system. All hardware components are directly controlled by ThorImage®LS software, including automated Z-step control for optical sectioning (via a piezo or stepper motor) and automatic calculation of Airy disk units based on the combination of the objective magnification and pinhole size; the intuitive interface allows novice and experienced users alike to obtain high-resolution microscope images quickly and easily.
Thorlabs' applications engineers install each confocal system and are available to address technical problems that may occur; for additional information, please see our Confocal Microscopy Contact Form. Also available is a confocal upgrade that combines Thorlabs' confocal package with third-party upright and inverted microscopes.
At the heart of our systems is a confocal scan head that can be configured with galvo-galvo or galvo-resontant scanners. Galvo-galvo scanners allow specific regions of interest to be targeted for experiments requiring photo-uncaging, while galvo-resonant scanners support high imaging speeds of up to 400 frames per second (at 512 x 32 pixel resolution). Both types of scanners can create full frame images with high spatial resolution of up to 4096 x 4096 pixels. At either extreme, or anywhere in-between, the control and acquisition system creates high-quality, jitter-free images.
The confocal scan path is integrated with a microscope body based on our Cerna Modular Microscopy System, allowing the system to be customized with widefield imaging accessories. A D1N dovetail on top of the scan path accepts single or double camera ports for scientific cameras, trinoculars, and epi-illuminators. A 95 mm dovetail along the length of the body accepts sample holders including a Z-axis piezo stage, condenser modules, or transmitted illumination modules for brightfield, Dodt contrast, or DIC imaging. In the most basic configuration, a mirror on a manual slider at the front of the scan path allows the user to switch between confocal and widefield imaging modalities. This optic can be upgraded to a dichroic or beamsplitter and the manual slider upgraded to a motorized one.
Our complete systems come standard with a primary dichroic that reflects four laser lines (405, 488, 532, and 642 nm). Other primary dichroics for use with other wavelengths can be provided upon request.
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The motorized filter wheel shown disconnected from the confocal scan head with the SMA fiber connector removed for free-space output.
Motorized Pinhole Wheel
For thicker samples, the size of the pinhole should be optimized relative to the NA of the objective in order to maximize signal to noise. With this in mind, our engineers selected each pinhole size to complement a common objective NA. Conversely, for thinner samples that produce less light outside of the focal plane, a larger pinhole size can help improve throughput. Pinhole diameters up to 2 mm provide flexibility so that the system can be easily adapted to different experiments.
A round pinhole is the ideal shape for maximizing the transmission of light generated in the focal plane of your sample while also optimizing the rejection of signal generated above and below the layer that is being scanned.
The pinhole is conveniently powered and controlled via USB. Additionally, the motorized, encoded control of the pinhole ensures perfect alignment and vibration-free movement. The emitted light from the specimen is focused on the pinhole and then collected by a large-core multimode fiber for transmission to the PMT detector system. More details on our motorized pinhole are available here.
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Four-Channel Laser Source
The solid state multi-laser source minimizes maintenance with an all-fiber design. Each laser line is individually fiber-coupled using a permanent rigid system. The individual fiber-coupled lasers are then combined in an all-fiber coupler with an FC/PC connector. This design ensures the lasers never go out of alignment by keeping the full power of the lasers coupled to the scan head at all times.
Each confocal system also includes a filter set, chosen to complement the excitation wavelengths. Available pre-configured laser source wavelength combinations and the included filter sets are outlined in the table below.
The laser source is controlled by a controller box, which includes a safety interlock system and manual control of laser emission. The laser source can also be controlled remotely by way of an auxiliary input.
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Expandable PMT modules are designed for multi-channel laser scanning microscopy applications. The photo shows the 4-channel module with standard sensitivity PMTs.
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Each Confocal System comes with a 64-bit computer tower with ThorImageLS installed and a 24" monitor for data acquisition and review.
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The filter sets are mounted in dichroic filter cubes for easy exchange. Here, an emission filter is being removed from a 2-channel PMT module with high-sensitivity PMTs.
Detector: Future-proof your experiments with our remotely positioned detector module that can be readily expanded from two to four photomultiplier tubes (PMTs). Sitting in front of each PMT is a quickly exchangeable dichroic mirror and emission filter holder (for included filter sets, see the table above).
The detection module can be configured with our standard sensitivity multialkali PMTs or high sensitivity, ultra-low-noise GaAsP PMTs. The standard sensitivity multi-alkali PMTs provide a low-noise image with high dynamic range that is appropriate for most life-science and industrial applications. For weakly fluorescent or highly photosensitive samples, we also offer the option of high-sensitivity, TEC-cooled GaAsP PMTs. With either choice, the gain of the detector as well as the dynamic range of the digitizer is controlled within the ThorImageLS software.
Filters: Each confocal system includes an appropriate set of emission filters that block laser light from entering the PMTs and provide pass bands at the fluorescence wavelengths of popular fluorophores. The exact configuration is determined by the laser wavelengths in your confocal system. Several common configurations and compatible fluorophores are outlined below. If you have questions concerning the filter set included with a specific laser configuration, please contact ImagingSales@thorlabs.com for more information.
Computer: Each confocal system includes a 64-bit computer with a 24" monitor. The included ThorImageLS software package provides an all-in-one solution for microscope control, automated data collection, and image review.
Sample Filter Configurations
In addition to the filter set list above, the two sample plots of emission filter pass bands are provided below as an additional example of how Thorlabs' Confocal System can work with popular fluorophores.
The primary dichroic and emission filter sets in the confocal system are typically optimized for one of two excitation wavelength configurations. The most popular configuration is compatible with 405 nm, 488 nm, 561 nm, and 642 nm excitation lasers. The graph to the lower left provides an example of this common emission filter configuration, with the emission spectra of four compatible fluorophores superimposed on the filters' transmission profiles.
The second most common 4-laser configuration exchanges the 561 nm laser for a 532 nm laser, useful for samples that are marked with TRITC. To accommodate this wavelength, the two bandpass filters centered at 525 nm and 593 nm would be replaced with a narrower bandpass filter (25 nm pass bandwidth) centered at 512 nm and a wider bandpass filter (75 nm pass bandwidth) centered at 582 nm.
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The solid lines in the plot show the pass bands of the filters, and the shaded areas show the normalized emission spectrum for the fluorophores.
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The solid lines in the plot show the pass bands of the filters, and the shaded areas show the normalized emission spectrum for the fluorophores.
Specifications for Thorlabs' Confocal System are provided in the tables below. For a description of the available modules, see the Confocal System tab. For more information, contact our sales team and applications engineers at ImagingSales@thorlabs.com or (703) 651-1700.
The laser wavelengths offered as part of the confocal system are available in one of 10 pre-configured integrated laser sources. Each source is paired with a set of filters optimized for popular fluorophores that can be mounted in the PMT detection module. If you need help selecting a laser source for you system, you can contact our applications engineers by e-mail at ImagingSales@thorlabs.com or by calling (703) 651-1700.
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Epi-Illuminator Module Add-On
Post-Installation Upgrades & Add-Ons
Each Four-Channel Confocal Microscope is designed so that imaging capabilities can be added or substituted after installation to accommodate new experimental needs as your research requirements grow. Some upgrades, such as adding Cerna® widefield imaging accessories, can be easily performed by the user. Others require replacing part of the hardware, in which case assistance will be provided by our technical staff; contact us using the link in the green box to the right for additional information.
Widefield Imaging Add-Ons
Confocal Capability Upgrades
Features of ThorImage®LS
Comprehensive Imaging Platform for:
Seamless Integration with Experiments
Advanced Software Functionality
The full source code for ThorImage®LS is available for owners of a Bergamo, Cerna, or confocal microscope. Click here to receive your copy.
ThorImageLS is an open-source image acquisition program that controls Thorlabs' Bergamo II, confocal microscopes, and Cerna® with hyperspectral imaging, as well as supplementary external hardware. From prepared-slice multiphoton Z-stacks to simultaneous in vivo photoactivation and imaging, ThorImageLS provides an integrated, modular workspace tailored to the individual needs of the scientist. Its workflow-oriented interface supports single image, Z-stacks, time series, and image streaming acquisition, visualization, and analysis. See the video at the top right for a real-time view of data acquisition and analysis with ThorImageLS.
ThorImageLS is included with a Thorlabs microscope purchase and open source, allowing full customization of software features and performance. ThorImageLS also includes Thorlabs’ customer support and regular software updates to continually meet the imaging demands of the scientific community.
For additional details, see the full web presentation.
Version 3.1 - October 25, 2017
Please contact ImagingTechSupport@thorlabs.com to obtain the latest ThorImageLS version compatible with your microscope. Because ThorImageLS 3.x adds significant new features over 2.x and 1.x versions, it may not be compatible with older microscopes. We continue to support older software versions for customers with older hardware.
New Hardware Support
User Interface (UI) Improvements
The goal of any microscope is to generate high-contrast, high-resolution images. In much the same way that a telescope allows scientists to discern the finest details of the universe, a microscope allows us to observe biological functioning at the nanometer scale. Modern laser scanning microscopes are capable of generating multidimensional data (X, Y, Z, τ, λ), leading to a plethora of high-resolution imaging capabilities that further the understanding of underlying biological processes.
In conventional widefield microscopy (Figure 1, below left), high-quality images can only be obtained when using thin specimens (on the order of one to two cell layers thick). However, many applications require imaging of thick samples, where volume datasets or selection of data from within a specific focal plane is desired. Conventional widefield microscopes are unable to address these needs.
LSM, in particular confocal LSM and multiphoton LSM, allows for the visualization of thin planes from within a thick bulk sample, a technique known as optical sectioning. In confocal LSM, signals generated by the sample outside of the optical focus are physically blocked by an aperture, preventing their detection. Multiphoton LSM, as we will discuss later, does not generate any appreciable signal outside of the focal plane. By combining optical sectioning with incremented changes in focus (Figure 2, below right), laser scanning microscopy techniques can recreate 3D representations of thick specimen.
Contrast Mechanisms in LSM
Biological samples typically do not have very good contrast, which leads to difficulty in observing the boundaries between adjacent structures. A common method for improving contrast in laser scanning microscopes is through the use of fluorescence.
In fluorescence, a light-emitting molecule is used to distinguish the constituent of interest from the background or neighboring structure. This molecule can already exist within the specimen (endogenous or auto-fluorescence), be applied externally and attached to the constituent (chemically or through antibody binding), or transfected (fluorescent proteins) into the cell.
In order for the molecule to emit light (fluoresce) it must first absorb light (a photon) with the appropriate amount of energy to promote the molecule from the ground state to the excited state, as seen in Figure 3A below. Light is emitted when the molecule returns back down to the ground state. The amount of fluorescence is proportional to the intensity (I) of the incident laser, and so confocal LSM is often referred to as a linear imaging technique. Natural losses within this relaxation process require that the emitted photon have lower energy—that is, a longer wavelength—than the absorbed photon.
Multiphoton excitation (Figure 3B, below) of the molecule occurs when two (or more) photons, whose sum energy satisfies the transition energy, arrive simultaneously. Consequently, the two arriving photons will be of lower energy than the emitted fluorescence photon.
There are also multiphoton contrast mechanisms, such as harmonic generation and sum frequency generation, that use non-absorptive processes. Under conditions in which harmonic generation is allowed, the incident photons are simultaneously annihilated and a new photon of the summed energy is created, as illustrated in Figure 3C below.
Further constituent discrimination can be obtained by observing the physical order of the harmonic generation. In the case of second harmonic generation (SHG), signal is only generated in constituents that are highly ordered and lacking inversion symmetry. Third harmonic generation (THG) is observed at boundary interfaces where there is a refractive index change. Two-photon excitation and SHG are nonlinear processes and the signal generated is dependent on the square of the intensity (I2).
The nonlinear nature of signal generation in multiphoton microscopy means that high photon densities are required to observe SHG and THG. In order to accomplish this while maintaining relatively low average power on the sample, mode-locked femtosecond pulsed lasers, particularly Ti:Sapphire lasers, have become the standard.
Another consideration to be made in nonlinear microscopy is the excitation wavelength for a particular fluorophore. One might think that the ideal excitation wavelength is twice that of the one-photon absorption peak. However, for most fluorophores, the excited state selection rules are different for one- and two-photon absorption.
This leads to two-photon absorption spectra that are quite different from their one-photon counterparts. Two-photon absorption spectra are often significantly broader (can be >100 nm) and do not follow smooth semi-Gaussian curves. The broad two-photon absorption spectrum of many fluorophores facilitates excitation of several fluorescent molecules with a single laser, allowing the observation of several constituents of interest simultaneously.
All of the fluorophores being excited do not have to have the same excitation peak, but should overlap each other and have a common excitation range. Multiple fluorophore excitation is typically accomplished by choosing a compromising wavelength that excites all fluorophores with acceptable levels of efficiency.
In a point-scanning LSM, the single-plane image is created by a point illumination source imaged to a diffraction-limited spot at the sample, which is then imaged to a point detector. Two-dimensional en face images are created by scanning the diffraction-limited spot across the specimen, point by point, to form a line, then line by line in a raster fashion.
The illuminated volume emits a signal which is imaged to a single-element detector. The most common single-element detector used is a photomultiplier tube (PMT), although in certain cases, avalanche photodiodes (APDs) can be used. CCD cameras are not typically used in point-scanning microscopes, though are the detector of choice in multifocal (i.e. spinning disk confocal) applications.
The signal from the detector is then passed to a computer which constructs a two-dimensional image as an array of intensities for each spot scanned across the sample. Because no true image is formed, LSM is referred to as a digital imaging technique. A clear advantage of single-point scanning and single-point detection is that the displayed image resolution, optical resolution, and scan field can be set to match a particular experimental requirement and are not predefined by the imaging optics of the system.
In confocal LSM, point illumination, typically from a single mode, optical-fiber-coupled CW laser, is the critical feature that allows optical sectioning. The light emitted from the core of the single mode optical fiber is collimated and used as the illumination beam for scanning. The scan system is then imaged to the back aperture of the objective lens which focuses the scanned beam to a diffraction-limited spot on the sample. The signal generated by the focused illumination beam is collected back through the objective and passed through the scan system.
After the scan system, the signal is separated from the illumination beam by a dichroic mirror and brought to a focus. The confocal pinhole is located at this focus. In this configuration, signals that are generated above or below the focal plane are blocked from passing through the pinhole, creating the optically sectioned image (Figure 2, above). The detector is placed after the confocal pinhole, as illustrated in Figure 4 to the right. It can be inferred that the size of the pinhole has direct consequences on the imaging capabilities (particularly, contrast, resolution and optical section thickness) of the confocal microscope.
The lateral resolution of a confocal microscope is determined by the ability of the system to create a diffraction-limited spot at the sample. Forming a diffraction-limited spot depends on the quality of the laser beam as well as that of the scan optics and objective lens.
The beam quality is typically ensured by using a single mode optical fiber to deliver the excitation laser light as a Gaussian point source, which is then collimated and focused into a diffraction-limited beam. In an aberration-free imaging system, obtained by using the highest quality optical elements, the size of this focus spot, assuming uniform illumination, is a function of excitation wavelength (λEX) and numerical aperture (NA) of the objective lens, as seen in Equation 1.
In actuality, the beam isn't focused to a true point, but rather to a bullseye-like shape. The spot size is the distance between the zeros of the Airy disk (diameter across the middle of the first ring around the center of the bullseye) and is termed one Airy Unit (AU). This will become important again later when we discuss pinhole sizes.
The lateral resolution of the imaging system is defined as the minimum distance between two points for them to be observed as two distinct entities. In confocal (and multiphoton) LSM, it is common and experimentally convenient to define the lateral resolution according to the full width at half maximum (FWHM) of the individual points that are observed.
Using the FWHM definition, in confocal LSM, the lateral resolution (Rlateral,confocal) is:
and the axial resolution (Raxial,confocal) is:
where n is the refractive index of the immersion medium.
It is interesting to note that in a confocal microscope, the lateral resolution is solely determined by the excitation wavelength. This is in contrast to widefield microscopy, where lateral resolution is determined only by emission wavelength.
To determine the appropriate size of the confocal pinhole, we multiply the excitation spot size by the total magnification of the microscope:
As an example, the appropriate size pinhole for a 60X objective with NA = 1.0 for λEX = 488 nm (Mscan head = 1.07 for the Thorlabs Confocal Scan Head) would be 38.2 μm and is termed a pinhole of 1 AU diameter. If we used the same objective parameters but changed the magnification to 40X, the appropriate pinhole size would be 25.5 μm and would also be termed a pinhole of 1 AU diameter. Therefore, defining a pinhole diameter in terms of AU is a means of normalizing pinhole diameter, even though one would have to change the pinhole selection for the two different objectives.
Theoretically, the total resolution of a confocal microscope is a function of the excitation illumination spot size and the detection pinhole size. This means that the resolution of the optical system can be improved by reducing the size of the pinhole. Practically speaking, as we restrict the pinhole diameter, we improve resolution and confocality, but we also reduce the amount of signal reaching the detector. A pinhole of 1 AU is a good balance between signal strength, resolution, and confocality.
In multiphoton LSM, a short pulsed free-space laser supplies the collimated illumination beam that passes through the scanning system and is focused by the objective. The very low probability of a multiphoton absorption event occurring, due to the I2 dependence of the signal on incident power, ensures signal is confined to the focal plane of the objective lens. Therefore, very little signal is generated from the regions above and below the focal plane. This effective elimination of out-of-focus signal provides inherent optical sectioning capabilities (Figure 2, above) without the need for a confocal pinhole. As a result of this configuration, the collected signal does not have to go back through the scanning system, allowing the detector to be placed as close to the objective as possible to maximize collection efficiency, as illustrated in Figure 5 to the right. A detector that collects signal before it travels back through the scan system is referred to as a non-descanned detector.
Again using the FWHM defintion, in multiphoton LSM, the lateral resolution (Rlateral,multiphoton) is:
and the axial resolution (Raxial,multiphoton) is:
These equations assume an objective NA > 0.7, which is true of virtually all multiphoton objectives.
The longer wavelength used for multiphoton excitation would lead one to believe (from Equation 5) that the resolution in multiphoton LSM, compared to confocal LSM, would be reduced roughly by a factor of two. For an ideal point object (i.e. a sub-resolution size fluorescent bead) the I2 signal dependence reduces the effective focal volume, more than offsetting the 2X increase in the focused illumination spot size.
We should note that the lateral and axial resolutions display a dependence on intensity. As laser power is increased, there is a corresponding increase in the probability of signal being generated within the diffraction-limited focal volume. In practice, the lateral resolution in a multiphoton microscope is limited by how tightly the illumination beam can be focused and is well approximated by Equation 5 at moderate intensities. Axial resolution will continue to degrade as excitation power is increased.
Although we are not directly rendering an image, it is still important to consider the size of the image field, the number of pixels in which we are displaying our image (capture resolution) on the screen, and the lateral resolution of the imaging system. We use the lateral resolution because we are rendering an en face image. In order to faithfully display the finest features the optical system is capable of resolving, we must appropriately match resolution (capture and lateral) with the scan field. Our capture resolution must, therefore, appropriately sample the optical resolution.
In LSM, we typically rely on Nyquist sampling rules, which state that the pixel size should be the lateral resolution divided by 2.3. This means that if we take our 60X objective from earlier, the lateral resolution is 249 nm (Equation 2) and the pixel size in the displayed image should be 108 nm. Therefore, for a 1024 x 1024 pixel capture resolution, the scan field on the specimen would be ~111 μm x 111 μm. It should be noted that the 40X objective from our previous example would yield the exact same scan field (both objectives have the same NA) in the sample. The only difference between the two images is the angle at which we tilt our scanners to acquire the image.
It may not always be necessary to render images with such high resolution. We can always make the trade-off of image resolution, scan field, and capture resolution to create a balance of signal, sample longevity, and resolution in our images.
Considerations in Live Cell Imaging
One of LSM's greatest attributes is its ability to image living cells and tissues. Unfortunately, some of the by-products of fluorescence can be cytotoxic. As such, there is a delicate balancing act between generating high-quality images and keeping cells alive.
One important consideration is fluorophore saturation. Saturation occurs when increasing the laser power does not provide the expected concurrent increase in the fluorescence signal. This can occur when as few as 10% of the fluorophores are in the excited state.
The reason behind saturation is the amount of time a fluorophore requires to relax back down to the ground state once excited. While the fluorescence pathways are relatively fast (hundreds of ps to a few ns), this represents only one relaxation mechanism. Triplet state conversion and nonradiative decay require significantly longer relaxation times. Furthermore, re-exciting a fluorophore before it has relaxed back down to the ground state can lead to irreversible bleaching of the fluorophore. Cells have their own intrinsic mechanisms for dealing with the cytotoxicity associated with fluorescence, so long as excitation occurs slowly.
One method to reduce photobleaching and the associated cytotoxicity is through fast scanning. While reducing the amount of time the laser spends on a single point in the image will proportionally decrease the amount of detected signal, it also reduces some of the bleaching mechanisms by allowing the fluorophore to completely relax back to the ground state before the laser is scanned back to that point. If the utmost in speed is not a critical issue, one can average several lines or complete frames and build up the signal lost from the shorter integration time.
The longer excitation wavelength and non-descanned detection ability of multiphoton LSM give the ability to image deeper within biological tissues. Longer wavelengths are less susceptible to scattering by the sample because of the inverse fourth power dependence (I-4) of scattering on wavelength. Typical penetration depths for multiphoton LSM are 250 - 500 μm, although imaging as deep as 1 mm has been reported in the literature, compared to ~100 μm for confocal LSM.
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This photo shows the male D1N dovetail on the trinoculars next to the female D1N dovetail on the epi-illumination arm.
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This photo shows the male 95 mm dovetail on the microscope body and the female 95 mm dovetail on the CSA1002 Fixed Arm.
Introduction to Microscope Dovetails
Dovetails are used for mechanical mating and optical port alignment of microscope components. Components are connected by inserting one dovetail into another, then tightening one or more locking setscrews on the female dovetail. Dovetails come in two shapes: linear and circular. Linear dovetails allow the mating components to slide before being locked down, providing flexible positioning options while limiting unneeded degrees of freedom. Circular dovetails align optical ports on different components, maintaining a single optical axis with minimal user intervention.
Thorlabs manufactures many components which use dovetails to mate with our own components or those of other manufacturers. To make it easier to identify dovetail compatibility, we have developed a set of dovetail designations. The naming convention of these designations is used only by Thorlabs and not other microscope manufacturers. The table to the right lists all the dovetails Thorlabs makes, along with their key dimensions.
In the case of Thorlabs’ Cerna® microscopes, different dovetail types are used on different sections of the microscope to ensure that only compatible components can be mated. For example, our WFA2002 Epi-Illuminator Module has a male D1N dovetail that mates with the female D1N dovetail on the microscope body's epi-illumination arm, while the CSS2001 XY Microscopy Stage has a female D1Y dovetail that mates with the male D1Y dovetail on the CSA1051 Mounting Arm.
To learn which dovetail type(s) are on a particular component, consult its mechanical drawing, available by clicking on the red Docs icon () below. For adapters with a female dovetail, the drawing also indicates the size of the hex key needed for the locking setscrew(s). It is important to note that mechanical compatibility does not ensure optical compatibility. Information on optical compatibility is available from Thorlabs' web presentations.
For customers interested in machining their own dovetails, the table to the right gives the outer diameter and angle (as defined by the drawings below) of each Thorlabs dovetail designation. However, the dovetail's height must be determined by the user, and for circular dovetails, the user must also determine the inner diameter and bore diameter. These quantities can vary for dovetails of the same type. One can use the intended mating part to verify compatibility.
In order to reduce wear and simplify connections, dovetails are often machined with chamfers, recesses, and other mechanical features. Some examples of these variations are shown by the drawings below.
Thorlabs' sales engineers and field service staff are based out of eight offices across four continents. We look forward to helping you determine the best imaging system to meet your specific experimental needs. Our customers are attempting to solve biology's most important problems; these endeavors require matching systems that drive industry standards for ease of use, reliability, and raw capability.
Thorlabs' worldwide network allows us to operate showrooms in a number of locations where you can see our systems in action. We welcome the opportunity for personal interaction during your visit! A demo can be scheduled at any of our showrooms by contacting ImagingSales@thorlabs.com.
Selected Confocal Microscopy Publications
Lewin, A. E. et al. Optogenetic and pharmacological evidence that somatostatin-GABA neurons are important regulators of parasympathetic outflow to the stomach. The Journal of Physiology n/a-n/a (2016). doi:10.1113/JP272069
Dechen, K., Richards, C. D., Lye, J. C., Hwang, J. E. C. & Burke, R. Compartmentalized zinc deficiency and toxicities caused by ZnT and Zip gene over expression result in specific phenotypes in Drosophila. The International Journal of Biochemistry & Cell Biology 60, 23–33 (2015).
Jia, Y. et al. Activation of platelet protease-activated receptor-1 induces epithelial-mesenchymal transition and chemotaxis of colon cancer cell line SW620. Oncol. Rep. 33, 2681–2688 (2015).
Lu, W. et al. Inhibiting the mobilization of Ly6Chigh monocytes after acute myocardial infarction enhances the efficiency of mesenchymal stromal cell transplantation and curbs myocardial remodeling. Am J Transl Res 7, 587–597 (2015).
Lu, W. et al. Photoluminescent Mesoporous Silicon Nanoparticles with siCCR2 Improve the Effects of Mesenchymal Stromal Cell Transplantation after Acute Myocardial Infarction. Theranostics 5, 1068–1082 (2015).
Zuo, S., Hughes, M. & Yang, G.-Z. Novel Balloon Surface Scanning Device for Intraoperative Breast Endomicroscopy. Ann Biomed Eng 1–14 (2015). doi:10.1007/s10439-015-1493-2
Brown, C. M., Melcher, J. T. & Stranick, S. J. Scan Linearization for Resonant Optomechanical Systems. in IM1C.3 (OSA, 2014). doi:10.1364/ISA.2014.IM1C.3
Partridge, J. G., Lewin, A. E., Yasko, J. R. & Vicini, S. Contrasting actions of group I metabotropic glutamate receptors in distinct mouse striatal neurones. J Physiol 592, 2721–2733 (2014).
Qin, X., Qiu, C. & Zhao, L. Maslinic acid protects vascular smooth muscle cells from oxidative stress through Akt/Nrf2/HO-1 pathway. Mol Cell Biochem 390, 61–67 (2014).
9.Liu, J. et al. Oleanolic Acid Suppresses Aerobic Glycolysis in Cancer Cells by Switching Pyruvate Kinase Type M Isoforms. PLOS ONE 9, e91606 (2014).
Hao, X. et al. Contrast reversal confocal microscopy. Optics Communications 298–299, 272–275 (2013).
Lalchandani, R. R., Goes, M.-S. van der, Partridge, J. G. & Vicini, S. Dopamine D2 Receptors Regulate Collateral Inhibition between Striatal Medium Spiny Neurons. J. Neurosci. 33, 14075–14086 (2013).
Standard Mechanical Interfaces on DIY Cerna® Components
The table below gives the dovetail, optical component threads, and cage system interfaces that are present on each DIY Cerna component. If a DIY Cerna component does not have one of the standard interfaces in the table, it is not listed here. Please note that mechanical compatibility does not ensure optical compatibility. Information on optical compatibility is available from Thorlabs' web presentations.