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60 mm Cage Cube for Fluorescence Filter Sets![]()
DFM2 60 mm Cage Cube for Fluorescence Filter Sets, SM2-Threaded Ports DFM2T2 Separate Inserts Available for DFM2B Kinematic Cage Cube Base Related Items ![]() Please Wait Fluorescence filter sets can be easily mounted in the filter cube (filters sold separately). ![]() Click to Enlarge The DFM2 has two excitation (EX) and two emission (EM) filter ports. Ports labeled with the same number should be used together. Features
The DFM2(/M) Fluorescence Filter Cube is designed to hold a fluorescence filter set (dichroic mirror, excitation filter, and emission filter) in a cage-system-compatible cube for home-built microscopy setups. It consists of a post-mountable base and removable insert that accepts filter set components. The cube insert is magnetically retained in the cube base, allowing for repeatable insertion in an aligned setup. Additional inserts are available to easily exchange alternate sets of optics within an aligned setup. This enables the user to switch between fluorescence imaging of different wavelengths. As shown in the animation to the right, the insert is designed to hold a rectangular optic, such as a dichroic mirror, beamsplitter, or silver mirror. The rectangular optic is clamped gently in place using a leaf spring that provides a uniform holding force to minimize stress. To mount the rectangular optic, remove the leaf spring using a Phillips #00 screwdriver and then insert the optic with the reflective coating face down. Only light force is needed to prevent the optic from moving; we recommend screwing in both screws most of the way before final tightening. In addition, the cube insert has four ports for Ø50 mm filters. Excitation and emission filters can be mounted in two possible configurations (see the diagram to the right) and are held using the included SM2RR Retaining Rings, which can be tightened or loosened with our SPW604 Spanner Wrench (sold separately). The emission ports (see photo to the right) are oriented at a 3° angle with respect to the face in order to reduce undesired reflections. To help keep track of your filter sets, spaces are provided on the top to write labels for the mounted filters and mirrors. The cube insert is designed to be inserted in a single orientation with respect to the cube base, so it is important to orient the full cube assembly in the proper direction within the setup.
The base of these kinematic cubes has an SM2-threaded (2.035"-40) port on each face at an optical height from the base of 1.75" and 45 mm for the imperial and metric versions, respectively. These ports are compatible with our SM2 lens tubes. Four 4-40 tapped holes surround each port for compatibility with our 60 mm cage system. The cube base is equipped with three bottom-located 1/4"-20 (M6) tapped holes, which provide direct compatibility with our Ø1" posts or Ø1.5" posts. Alternately, an AE8E25E (AE4M6M) thread adapter (sold separately) can be used for compatibility with our Ø1/2" posts.
DFM1 Filter Cubes - Optic Alignment in a Confocal Microscope Detection ModuleThe DFM1 is ideal for integrating filter sets into a custom microscopy setup. The following example of a partial confocal microscope uses a DFM1 Fluorescence Filter Cube (denoted by 1 in the image below) containing a Microscopy Filter Set (2) and an APD130A(/M) Photodetector (8). A lens (5) located inside the SM1ZM High-Precision Zoom Housing (4) focuses the beam onto a precision pinhole (7) located just before the avalanche photodetector's detector element. The SM1ZM zoom housing is mounted directly onto the ST1XY-D XY Translation Mount with Differential Adjusters (6), creating a compact XYZ mount. SM1 Lens Tubes and the SM1B3 Lens Tube Bellows (3) allow for a light-tight connection without inhibiting the free movement of the XYZ mount. Components used in the configuration pictured below are listed in the table to the right. These parts, along with SM1 Lens Tubes and 1" Post Assemblies, are sold separately. ![]()
![]() ![]() Click for Details Drawing of DFM2(/M)
The Kinematic Fluorescence Filter Cube for 60 mm Cage Systems consists of a post-mountable cube base and a removable insert that mounts a fluorescence filter set (not included). See the table to the right for acceptable optic sizes. To see how to install optics in the filter cubes, please see the animation in the Overview tab above. Prior to mounting the rectangular dichroic mirror, please confirm that the optic is no greater than 3.0 mm thick. The cube insert is designed to be inserted in a single orientation with respect to the cube base, so it is important to orient the full cube assembly in the proper direction. All four sides of the filter cube base have an SM2-threaded port for compatibility with our Ø2" lens tubes. The SM2 port is centered between four 4-40 tapped holes for compatibility with Ø6 mm cage rods in a 60 mm cage system, at an optical axis height of 1.75" or 45.0 mm in the imperial or metric versions, respectively. An empty kinematic filter cube insert, base, and cover plate are also sold separately below. ![]()
The Kinematic Fluorescence Filter Cube Insert (DFM2T1) and Base [DFM2B(/M)] are ideal for customers who wish to mount their own filter sets. Different filter sets can be loaded into the inserts, allowing for easy exchange of filter sets within an experimental setup (see image to the right). These items do not include fluorescence filters or a dichroic mirror. See the table to the right for acceptable optic sizes. To see how to install optics in the cube, please see the animation in the Overview tab above. Prior to mounting the rectangular dichroic mirror, please confirm that the optic is no greater than 3.0 mm thick. The cube insert is designed to be inserted in a single orientation with respect to the cube base, so it is important to orient the full cube assembly in the proper direction. All four sides of the DFM2B(/M) cube base have an SM2-threaded port for compatibility with our Ø2" lens tubes. The SM2 port is centered between four 4-40 tapped holes for compatibility with Ø6 mm cage rods in a 60 mm cage system, at an optical axis height of 1.75" or 45.0 mm from the bottom of the imperial or metric cube, respectively. For when a filter is not installed within the DFM2B(/M) cube base, we have the DFM2C Cage Cube Cover Plate to place on top. Additionally, we offer the SM2CP2 End Cap to minimize the amount of stray light entering through unused ports. ![]() ![]() Click to Enlarge Two 60 mm Cage-Compatible Beam Turning Cubes Connected Using the DFM2-CC Cage Cube Connector
The DFM2-CC Cage Cube Connector allows two kinematic 60 mm cage cubes to be connected together, as shown in the image to the right. The connector is attached to the cage cubes using the eight included 4-40 screws (1/16" hex key included) in the cage rod holes. Two pins on the DFM2-CC are accepted by drilled holes on either side of any of the SM2-threaded (2.035"-40) ports on the cage cube faces to maintain alignment. Fluorescence filter and beam turning cube inserts can still be removed and inserted while the cube bases are connected. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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