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Bergamo® II Series Multiphoton Microscopes


Bergamo® II Series Multiphoton Microscopes


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The Bergamo® II Series is Thorlabs' platform for multiphoton microscopy. Following the principle that the microscope should conform to the specimen, rather than the other way around, we created a completely modular imaging platform that adapts to a wide range of experimental requirements and can be easily upgraded as experimental needs evolve.

Options at a Glance

Laser Scanning

  • 8 kHz and 12 kHz Galvo-Resonant Scanners for High-Speed Imaging
  • Galvo-Galvo Scanners for User-Defined ROI Shapes and Photostimulation Patterns
  • Spatial Light Modulator for Simultaneous Targeting
  • Super Broadband Scan Optics Optimized for:
    • Photoactivation / Uncaging
    • Two-Photon Imaging
    • Three-Photon Imaging
  • Co-Registered Confocal Imaging

Microscope Body

  • Rotating Bodies
    • 5" of Coarse Vertical Motion
    • -5° to +95° or -50° to +50° Rotation Around the Sample
      (-45° to +45° Only with SLM)
    • 2" of Fine XY Motion
    • 1" of Fine Z Motion
    • X, Y, and Z Rotate with Objective
  • Upright Bodies
    • Fine Z or Fine XYZ Motion

Signal Detection

  • Up to Four Simultaneous Detection Channels
  • Thermoelectrically Cooled or Large-Angle Non-Cooled GaAsP PMTs
  • 8°, 10°, or 14° Collection Optics in Epi Direction (for Ø20 mm Entrance Pupil)
  • Mechanical Shutters Available for Photostimulation
  • Option for Two Forward-Direction
    Detection Channels
  • Easy-to-Exchange Magnetic Filter Holders

Transmitted Light Imaging

  • Dodt Gradient Contrast (Widefield and
    Laser Scanned)
  • DIC (Widefield and Laser Scanned)
  • Illumination Modules are Easily Removed for In Vivo Experiments
  • Visible and NIR LED Illumination
  • Available for Rotating and Upright Bodies
Sam Rubin
Sam Rubin
Imaging Systems General Manager

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Questions?
Need a Quote?

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Images Taken by Thorlabs' Microscopes

Rotating Microscopes: The Flexibility to Bring the Objective to the Region of Interest

Bergamo II Rotating Body
Click to Enlarge

Dual-scan-path systems, like the one shown above, rotate from -5° to +95°.
Single-scan-path systems can be factory configured for -5° to +95° or -50° to +50°.
Systems with a Spatial Light Modulator may have -45° to +45° rotation.

Like the original Bergamo® microscope, the Bergamo® II is available with a five-axis motorized body (X, Y, Z, θ, and microscope base) that translates the entire optical path, rotates around the sample position, and creates a large volume underneath the objective for complex experimental apparatuses.

Bergamo II systems with rotation can be equipped with a -5° to +95° range (for single and dual scan path configurations), a -50° to +50° range (for single scan path configurations only), or a -45° to +45° range (for configurations with our Spatial Light Modulator). Since the microscope moves the entire optical system, it is not necessary to reposition the sample, adjust the focus, or re-locate the region of interest after the objective is rotated. An integrated elevator coarsely moves the objective over a 5" (12.7 cm) range in the vertical direction, supporting experimental setups and apparatuses of varying sizes.

Motorizing the optical system not only simplifies experiments for the user but also helps ensure that the designed optical performance of your microscope and your objectives is achieved. Our articulating periscope, detailed in the Modules tab, is a key feature of our rotating design.

Five-Axis Motorization of Rotating Bergamo II Microscopes

 

Rotating Bergamo® II System Examples

Configuration B248
Rotating Premium In Vivo Microscope
Bergamo II Configuration B248
Configuration B221
Rotating Mid-Level In Vivo Microscope
Bergamo II Configuration B221
Configuration B209
Rotating Entry-Level In Vivo Microscope

Bergamo II Configuration B209

Thorlabs recognizes that each imaging application has unique requirements.
If you have any feedback, questions, or need a quotation, please use our
multiphoton microscopy contact form or call (703) 651-1700.

Upright Microscopes: In Vivo and Slice Rigs

Upright Bergamo® II microscopes offer one or three axes of motion and an industry-leading throat depth. Designed around Thorlabs' 95 mm construction rails, they offer stable long-term support and excellent vibrational damping. They offer a linear dovetail mounting surface that accepts our user-installable transmitted light illumination modules.

For slice imaging with brightfield illumination, Dodt gradient contrast, or DIC, simply attach the modules to the rail, using a balldriver to tighten a few setscrews in place. To free room underneath the objective for large in vivo specimens, loosen the setscrews and remove the modules. The installation and removal process takes fewer than 5 minutes.

Our transmitted light imaging modules are compatible with industry-standard condensers from Nikon.

BergamoII Non-Rotating Body
Click to Enlarge

Installing the Transmitted Light Modules Enables Dodt Contrast,
Laser-Scanned Dodt Contrast, or DIC Imaging
BergamoII Non-Rotating Body
Click to Enlarge

Removing the Transmitted Light Modules Makes Room
Underneath the Objective for Live Animal Imaging

 

Non-Rotating Bergamo® II System Examples

Configuration B232
Mid-Level In Vitro and In Vivo Microscope
with Z-Axis Motion and XY Platform
BergamoII Configuration B232
Configuration B206
Entry-Level In Vitro and In Vivo Microscope with Z-Axis Motion
BergamoII Configuration B206
Configuration B264
Premium In Vitro and In Vivo Microscope with XYZ Motion
BergamoII Configuration B264
Configuration B203
Entry-Level In Vitro Microscope with XYZ Motion
BergamoII Configuration B203

Thorlabs recognizes that each imaging application has unique requirements.
If you have any feedback, questions, or need a quotation, please use our
multiphoton microscopy contact form or call (703) 651-1700.

Features

The features of Bergamo II listed below, spanning imaging functionality, optical performance, day-to-day usage, beam conditioning, sample holders, and Thorlabs support, reflect our focus to develop cutting-edge capabilities without compromising usability. Many of these features can also be added to existing Thorlabs microscope systems. For more information, please see the Retrofits tab.

Laser Scanning, Widefield Imaging, and Transmitted Light Imaging
Scan Paths Galvo-Resonant
  • 8 kHz Scanner: Image at 2 fps (4096 x 4096 Pixels), 30 fps (512 x 512 Pixels), or
    400 fps (512 x 32 Pixels)
  • 12 kHz Scanner: Image at 45 fps (512 x 512 Pixels) or 600 fps (512 x 32 Pixels)
Galvo-Galvo
  • User-Defined Scan Geometries: Squares, Rectangles, Circles, Ellipses, Lines, and Polylines
  • Capture Weak Signals with Long Dwell Time Integration
  • Consistent Dwell Times Across Field of View
  • 48 fps at 512 x 32 Pixels and 70 fps at 32 x 32 Pixels
Spatial Light Modulator
  • Simultaneous Multi-Site Photostimulation using Holographic Beam Control
  • Entirely Controlled Through ThorImage®LS
  • Precise Control Along Z-Axis of Photoexcited Locations
Widefield Viewing
  • Thorlabs or Third-Party Scientific Cameras
    • Visualize Samples with Fluorescence or Reflected Light
    • Compatible with C-Mount-Threaded Scientific Cameras
  • Single- or Multi-Filter-Cube Epi-Illuminator Module
    • Locate Areas of Interest Without Unnecessary Laser Excitation
    • Thorlabs' LEDs Provide Bright Illumination
    • Industry-Standard Liquid Light Guides Illuminate Across the Visible Spectrum
Dodt Gradient Contrast and DIC Modules
  • User-Removable Modules Convert Microscope Between In Vivo and Slice Imaging
  • Camera or Laser Scanned
  • Dodt Gradient Contrast: View Features in Tissue Slices
  • DIC: View Features in Thinner, Transparent Samples
Optical Performance
Choice of Detectors
  • Choice of Signal Collection Angles to Accommodate Different Sample Depths
    • 8° (For Two PMTs)
    • 10° (For up to Four PMTs)
    • 14° (For Two PMTs)
    • Angles Quoted for an Objective with a Ø20 mm Entrance Pupil
  • Available With or Without Mechanical Shutters for Photoactivation Experiments
  • Option for Two Highly Sensitive Forward Fluorescence Detection Channels
Minimal Distance Between Objective and First Collecting Lens
  • Large Collection Angle for Multiphoton Fluorescence Emission
  • Increased Collection Efficiency
Periscopes
  • Maintain Optimal Laser Alignment and Optical Performance over Microscope's Entire Travel Range
Scan Paths Designed in House
  • Super Broadband Correction Range of 450 - 1100 nm, 680 - 1600 nm, or 900 - 1900 nm
  • Optimized for Photostimulation, Two-Photon Imaging, or Three-Photon Imaging
  • Specifically Designed to Match the Low-Magnification, High-NA Objectives Popularly Used in Multiphoton Microscopy
  • Take Full Advantage of the Latest Widely Tunable Ti:Sapphire and OPO Systems
  • Fill Up to a Ø20 mm Back Aperture Objective
  • Up to F.N. 20
Day-to-Day Usage
Several Software Packages
  • ThorImage®LS: Our Internally Developed, Open-Source Solution
  • Full SDK for LabVIEW and C++ Available Upon Request
  • Compatible with ScanImage
Touchscreen Controller
  • Touchscreen Shows Current Position of All Axes
  • Tap to Save and Retrieve Two Positions in Space
Easy-to-Reach Emission Filters and Dichroic Holders
  • Filters are Accessed from the Front of the Microscope and Take Less than 5 Minutes to Exchange
Input and Output Triggers
  • Use Electrical Signals to Synchronize All Your Equipment
  • Input Triggers can Start a Single Series or an Indefinite Series
  • Output Triggers can be Sent at the Beginning of a Frame or Line
  • High-Bandwidth Signal Integration with Electrophysiology
Large User-Adjustable Volume Underneath Objective
  • Accommodates Large Preps and Setups
  • Approach Angles Around Objective are Not Restricted
  • Rotating Bodies have 5" (12.7 cm) of Elevator Travel for Easily Adapting the Microscope for Differently Sized Experimental Setups
-5° to +95° or -50° to +50° Rotation of the Focal Plane (Rotating Bodies Only)
  • Image Different Sections of the Brain without Having to Move Your Specimen or Refocus the Objective
Beam Conditioning Modules
Pockels Cells
  • Edge and Fly-Back Blanking to Minimize Sample Photobleaching
  • High-Speed Masking for ROIs
  • Customize Laser Power at Each Slice Using Software Control
Variable Attenuator
  • Manual and Computer Control of Laser Power in Systems Without a Pockels Cell
  • Improves Pockels Cell Performance
  • One-Click Shutter
Variable Beam Expander
  • 1X - 3X Beam Diameter Modulation into the Objective Back Aperture Using Software Control
Beam Stabilizer
  • Maintain Stable Beam Pointing While Changing the Excitation Laser Wavelength
Sample Holders
Rigid Stands for Slides, Recording Chambers, or Platforms
  • Minimal Footprint Conserves Space Around Objective and the Microscope
  • Slim Profile Leaves Room for Dodt or DIC Imaging Modules
  • Excellent Long-Term Stability
  • Easily Rotate Samples Into and Out of the Beam Path
XY Platforms for Micromanipulators
  • Large Working Space that Surrounds the Objective on Three Sides
  • Ideal for Setups Where the Sample and Apparatus Need to Move in Unison, Such as Patch Clamping
  • 2" Travel in X and Y; 0.5 µm Encoder Resolution
Thorlabs Support
Fully Designed and Manufactured in House
  • Engineers Work Under One Roof to Lower Your Costs and Create Seamless Solutions
  • Expertise in Every System Component
Modular System Construction
  • As Your Experimental Needs Evolve, Upgrade Your Microscope Without Sacrificing Existing Capabilities
Professional Installation
  • Thorlabs Technician Visits Your Lab to Assemble, Test, and Demonstrate Use of Your Microscope
Quick Support
  • Communicate with Our Support Staff Faster than an Engineer Could Travel to Your Location
  • Thorlabs Technicians and Application Specialists Available for Videoconferencing
  • Thorlabs Will Ship You a Camera with a Microphone to Facilitate the Conversation
  • With Permission, Thorlabs Will Remote Desktop in to Address Software Issues

Thorlabs recognizes that each imaging application has unique requirements.
If you have any feedback, questions, or need a quotation, please use our
multiphoton microscopy contact form or call (703) 651-1700.

Bergamo® II Modules in Detail

BergamoII Rotating Body

Galvo-Resonant Scanners, Galvo-Galvo Scanners, and Spatial Light Modulators

Bergamo® II microscopes can be configured with a galvo-resonant scan path, a galvo-galvo scan path, and/or a spatial light modulator (SLM). These choices allow the user to optimize each experiment as needed for high frame rates, high sensitivity, and/or targeted exposure of the regions of interest.

Galvo-Resonant Scanners for High-Speed Imaging
Thorlabs offers 8 kHz and 12 kHz galvo-resonant scanners. Our 8 kHz scanners utilize the entire field of view and offer a maximum frame rate of 400 fps, while our 12 kHz scanners provide an increased frame rate of 600 fps.

Galvo-Galvo Scanners for User-Defined ROI Shapes
Galvo-galvo scanners support user-drawn scan geometries (lines, polylines, squares, and rectangles) and also support custom photoactivation patterns (circles, ellipses, polygons, and points). They offer consistent pixel dwell times for better signal integration and image uniformity.

Spatial Light Modulator for Simultaneous Targeting
Unlike scanners, which physically move from point to point, spatial light modulators (SLMs) use holography to direct the beam. This allows multiple sites in a sample to be photoexcited truly simultaneously.

Example Configurations with Dual Galvo-Resonant
and Galvo-Galvo Scan Paths
Configuration B264: Premium In Vitro and In Vivo Microscope with XYZ Motion
Configuration B248: Rotating Premium In Vivo Microscope
Example Configurations with a 
Galvo-Resonant Scan Path Only
Configuration B209: Rotating Entry-Level In Vivo Microscope
Configuration B221: Rotating Mid-Level In Vivo Microscope
Configuration B206: Entry-Level In Vitro and In Vivo Microscope with
Z-Axis Motion
Configuration B203: Entry-Level In Vitro Microscope with XYZ Motion
Example Configuration with a
Galvo-Galvo Scan Path Only
Configuration B232: Mid-Level In Vitro and In Vivo Microscope with Z-Axis Motion
and XY Platform

 

Figure 3. 1/16 Speed Wavelength Switching Using Tiberius Ti:Sapphire Laser
Fast Switching
Click to Enlarge
Figure 2. Composite Image Acquired Using Fast Switching between 750 nm and 835 nm and 7 fps image rate.
Fast Switching
Click to Enlarge
Figure 1. Composite Image Acquired Using Single Excitation at 788 nm

Fast Switching Using Tunable Femtosecond Laser

With an industry-leading tuning speed of up to <50 ms over a 200 nm range, the Tiberius® Ti:Sapphire Femtosecond Laser is ideal for fast sequential imaging in multiphoton microscopy applications. 

The images and video show the fluorescence excitation of a 25 µm thick adult rat brain sagittal section. The red channel corresponds to fluorescence from chick anti-neurofilament that is optimally excited at 835 nm, while the green channel corresponds to fluorescence from mouse anti-GFAP that is optimally excited at 750 nm.

Figure 1 is a composite image showing single-wavelength excitation at 788 nm, while Figure 2 is a composite of fast switching between 750 nm and 835 nm. At full speed, both channels are collected at an imaging rate of 7 fps; Figure 3 shows each channel being acquired at 1/16 of full speed. When compared to single-wavelength excitation at 788 nm with the same intensity, fast switching offers much higher image contrast.

This immunofluorescence sample was prepared by Lynne Holtzclaw of the NICHD Microscopy and Imaging Core Facility, a part of the National Institutes of Health (NIH) in Bethesda, MD.

 

BergamoII Rotating Body
Click to Enlarge

Rotating Bergamo II systems are outfitted with multi-joint articulating periscopes. This periscope's design offers the enhanced flexibility needed to allow the entire scanning system to be tilted with respect to the sample.
BergamoII Non-Rotating Body
Click to Enlarge

Upright Bergamo II systems are equipped with periscopes that permit the microscope's full travel range in X, Y, and Z to be used without compromising the optical performance.

Periscopes

Most lasers used in multiphoton microscopy are delivered by a free-space beam. To stay aligned and optimized, the beam path must remain fixed. The Bergamo® II's ability to translate the objective around the focal plane in up to five axes (X, Y, Z, θ, and microscope base) also requires the beam path to translate along the same axes. Bergamo II systems overcome this engineering challenge using multi-jointed periscopes.

Example Configurations with Articulating Periscopes
Configuration B209: Rotating Entry-Level In Vivo Microscope
Configuration B221: Rotating Mid-Level In Vivo Microscope
Configuration B248: Rotating Premium In Vivo Microscope
Example Configurations with Fixed Periscopes
Configuration B206: Entry-Level In Vitro and In Vivo Microscope with
Z-Axis Motion
Configuration B232: Mid-Level In Vitro and In Vivo Microscope with
Z-Axis Motion and XY Platform
Configuration B203: Entry-Level In Vitro Microscope with XYZ Motion
Configuration B264: Premium In Vitro and In Vivo Microscope with XYZ Motion

 

BergamoII Filter Exchange
Click to Enlarge

The emission filters and dichroic cubes are held behind magnetically sealed doors on the front of the PMT detection module.

Super Broadband Scan Optics

Bergamo® II microscopes feature proprietary scan optics that are optimized and corrected for excitation wavelengths within the 450 - 1100 nm, 680 - 1600 nm, or 900 - 1900 nm wavelength range, ideal for photostimulation, two-photon imaging, and three-photon imaging, respectively. These broad ranges, extending from the visible well into the near infrared, were chosen to support the latest widely tunable Ti:Sapphire lasers and OPO systems, as well as dual-output lasers such as the Chameleon Discovery.

Our optics take full advantage of the optical designs used in the low-magnification, high-numerical-aperture objectives that have become the workhorses of multiphoton microscopy. They allow the back aperture of the objective to be filled up to Ø20 mm while providing a scan area that is 1.6X larger than that offered on our original Acerra and Bergamo multiphoton microscopes. This expanded area lets you find a region of interest more quickly or simply image more cells at once.

Detectors in Epi and Transmitted Directions

We employ high-sensitivity GaAsP PMTs in our multiphoton systems. These detectors offer high quantum efficiency, aiding in imaging weakly fluorescent or highly photosensitive samples. Two types are available: a thermoelectrically cooled PMT for improved sensitivity toward weak signals, and a non-cooled PMT that offers a smaller package size and greater numerical aperture.

All Bergamo® II microscopes can be equipped with two or four detection channels in the epi direction, and/or two detection channels in the forward direction. The user can configure the forward-direction channels to detect the same fluorescent tags as the epi-direction PMTs, raising the microscope's sensitivity toward thin, weakly fluorescent specimens.

A maximum of four channels can be controlled by the software at a given time.

Easy-to-Reach Emission Filters and Dichroic Holders

Bergamo® II systems are fully compatible with industry-standard fluorescence filter sets that include Ø25 mm fluorescence filters and 25 mm x 36 mm dichroic mirrors. Unlike competing designs, Thorlabs' detector modules have magnetic holders that make it simple and quick to exchange filters for different measurements.

We also offer detection modules for large-area Ø32 mm fluorescence filters and 32 mm x 42 mm dichroics, which support greater collection angles for increased signal.

 

Multi-Axis Controller with Touchscreen

This controller is specifically designed for rotating Bergamo® II microscope bodies. It uses knobs to control up to five motorized axes. On rotating systems, a rocker switch changes between fine objective focusing and translation of the elevator base. Each axis can be disabled on an individual basis in order to maintain a location along the desired direction.

The integrated touchscreen lets two spatial locations be saved and retrieved locally. Up to eight spatial locations can be saved on the computer running ThorImage®LS. The touchscreen also reads out the position of every motor.

 

Objectives

Bergamo® II microscopes accept infinity-corrected objectives with M34 x 1.0, M32 x 0.75, M25 x 0.75, or RMS threads. Together, these options encompass the majority of low-magnification, high-NA objectives used in multiphoton microscopy. With a large field number of 20, our scan optics completely utilize the optical designs of these specialized objectives, offering enhanced light-gathering ability compared to competing microscopes using the same objectives.

 

Rigid Stand Sample Holders

Thorlabs' Rigid Stands are rotatable, lockable, low-profile platforms for mounting slides, recording chambers, our Z-axis piezo stages, and custom experimental apparatuses. Each fixture is supported by a solid Ø1.5" stainless steel post for passive vibrational damping, which is in turn held to the workstation by the red post holder.

A locking collar maintains the height of the platform, allowing it to easily rotate into and out of the optical path, and a quick-release mechanism holds the post in place once the desired position is achieved.

 

BergamoII Rotating Body
Click to Enlarge

Four Epi-Direction PMTs with 10° Collection Optics and Mechanical Shutters

Large-Angle Signal Collection Optics

Deriving the most signal from limited photons is the fundamental goal of any detection system. To this end, we employ ultrasensitive GaAsP PMTs that offer high quantum efficiency. By positioning the PMTs immediately after the objective (a "non-descanned" geometry), light that is scattered by the sample, which therefore appears to originate outside the objective's field of view, still strikes the PMTs and adds to the collected signal. This is a benefit unique to multiphoton microscopy. Collecting beyond the objective's design field of view greatly enhances overall detection efficiency when imaging deep in tissue.

In the epi direction, we offer signal collection angles of 8°, 10°, or 14° (for a Ø20 mm entrance pupil), while in the transmtited direction, we offer signal collection angles of 13°. Our collection modules can optionally be outfitted with mechanical shutters for photoactivation experiments.

Example Configurations with Full-Field-of-View
Collection Optics
Configuration B203: Entry-Level In Vitro Microscope with XYZ Motion
Configuration B209: Rotating Entry-Level In Vivo Microscope
Example Configurations with Extended-Field-of-View
Collection Optics
Configuration B221: Rotating Mid-Level In Vivo Microscope
Configuration B248: Rotating Premium In Vivo Microscope
Configuration B206: Entry-Level In Vitro and In Vivo Microscope with
Z-Axis Motion
Configuration B232: Mid-Level In Vitro and In Vivo Microscope with Z-Axis Motion
and XY Platform
Configuration B264: Premium In Vitro and In Vivo Microscope with
XYZ Motion

 

Bergamo II with Dodt Contrast
Click to Enlarge
Dodt Contrast Module, Motorized Condenser Stage, and
Rigid Stand Sample Holder Underneath the Objective

User-Installable Dodt Contrast and DIC Imaging Modules

The modular construction of the Bergamo® II makes it exceptionally easy for the user to convert the microscope between in vitro and in vivo applications. Our user-installable Dodt contrast, laser-scanned Dodt contrast, and DIC modules take less than 5 minutes to attach or remove from the microscope body. These modules are available for both rotating and upright bodies.

Each option is paired with our basic 3-axis controller, which optimizes the illumination conditions by translating our motorized condenser stage over a 1" range. This versatile design is compatible with air and high-NA oil immersion condensers designed by Nikon.

To complement these modules, we manufacture slim-profile rigid stand sample holders that are ideal for positioning slides between the transmitted light module and the objective.

Example Configuration with Laser-Scanned Dodt Contrast
Configuration B264: Premium In Vitro and In Vivo Microscope with XYZ Motion

 

BergamoII Scientific Cameras
Click to Enlarge

1.4 Megapixel Scientific Camera

Scientific Cameras

Our low-noise, scientific-grade CCD cameras were designed for full compatibility with Thorlabs’ multiphoton microscopy systems. Useful for widefield and fluorescence microscopy, they are capable of visualizing in vitro and in vivo samples using reflected light and fluorescence emission. They work in conjunction with the epi-fluorescence module to help locate fiducial markers, and they also enable imaging modalities that do not require laser exposure.

Thorlabs' cameras are driven by our internally developed ThorCam software package, and are available in 1.4 megapixel, 4 megapixel, 8 megapixel, and fast-frame-rate versions. Generally speaking, cameras with lower resolution offer higher maximum frame rates. These cameras also feature a separate auxiliary port that permits the image acquisition to be driven by an external electrical trigger signal.

Bergamo® II microscopes are also directly compatible with any camera using industry-standard C-mount or CS-mount threads.

Thorlabs recognizes that each imaging application has unique requirements.
If you have any feedback, questions, or need a quotation, please use our
multiphoton microscopy contact form or call (703) 651-1700.

Configurations

Explore the details of highlighted Bergamo II system configurations by clicking on the expandable sections below. Due to the modular nature of our multiphoton microscopy platform, these configurations may be easily tailored to meet individual experimental needs.

Thorlabs recognizes that each imaging application has unique requirements.
If you have any feedback, questions, or need a quotation, please use our
multiphoton microscopy contact form or call (703) 651-1700.

Sam Rubin
Sam Rubin
Imaging Systems General Manager

Questions?
Need a Quote?

Contact Me
Trans-Illumination Path Add-On for Rotating Bergamo Systems
Click to Enlarge
Trans-Illumination Add-On for Rotating Bergamo II Systems

Retrofit Options for Existing Multiphoton Systems

  • Upgrades to the Functionality of Existing Microscope Infrastructure and Components
  • Add-On Components to Increase Capabilities

Thorlabs' modular design enables our microscopes to continually evolve with experimental needs. Customers with previous model microscopes and product lines for multiphoton microscopy have the flexibility to update their infrustructure or add to their existing components as their microscopy needs change. See the expandable tables below for options available for each of our multiphoton system lines, and contact us for additional information about incorporating an upgrade or add-on into your system.

Please note that certain upgrades and add-ons require an on-site visit by one of our specialists for installation.

Bergamo II Compatible Upgrades and Add-Ons
Bergamo I (B-Scope) Compatible Upgrades and Add-Ons
Acerra (A-Scope) Compatible Upgrades and Add-Ons

ThorImage®LS Software

(Click Here for Full Web Presentation)


Features of ThorImage®LS

Comprehensive Imaging Platform for:

Seamless Integration with Experiments

  • Simultaneous Multi-Point Photoactivation and Imaging with Spatial Light Modulator
  • Fast Z Volume Acquisition with PFM450E or Third-Party Objective Scanners
  • Electrophysiology Signaling
  • Wavelength Switching with Tiberius® Laser or Coherent Chameleon Lasers
  • Pockels Cell ROI Masking
  • Power Ramped with Depth to Minimize Damage and Maximize Signal-to-Noise

Advanced Software Functionality

  • Multi-Column Customizable Workspace
  • Image Acquisition Synced with Hardware Inputs and Timing Events
  • Live Image Correction and ROI Analysis
  • Independent Galvo-Galvo and Galvo-Resonant Scan Areas and Geometries
  • Tiling for High-Resolution Large-Area Imaging
  • Independent Primary and Secondary Z-Axis Control for Fast Deep-Tissue Scans
  • Automated Image Capture with Scripts
    • Compatible with ImageJ Macros
  • Multi-User Settings Saved for Shared Workstations
  • Individual Colors for Detection Channels Enable Simple Visual Analysis

 

The full source code for ThorImage®LS is available for owners of a Bergamo, Cerna, or confocal microscope. Click here to receive your copy.

ThorImageLS Brochure

ThorImageLS is an open-source image acquisition program that controls Thorlabs' Bergamo II, confocal microscopes, and Cerna® with hyperspectral imaging, as well as supplementary external hardware. From prepared-slice multiphoton Z-stacks to simultaneous in vivo photoactivation and imaging, ThorImageLS provides an integrated, modular workspace tailored to the individual needs of the scientist. Its workflow-oriented interface supports single image, Z-stacks, time series, and image streaming acquisition, vizualization, and analysis. See the video at the top right for a real-time view of data acquisition and analysis with ThorImageLS.

ThorImageLS is included with a Thorlabs microscope purchase and open source, allowing full customization of software features and performance. ThorImageLS also includes Thorlabs’ customer support and regular software updates to continually meet the imaging demands of the scientific community.

For additional details, see the full web presentation.

 

New Functionality

Version 3.1 - October 25, 2017

Please contact ImagingTechSupport@thorlabs.com to obtain the latest ThorImageLS version compatible with your microscope. Because ThorImageLS 3.x adds significant new features over 2.x and 1.x versions, it may not be compatible with older microscopes. We continue to support older software versions for customers with older hardware.

New Hardware Support
  • Added Support for Thorlabs' Scientific Monochrome CCD Cameras; Allows Imaging with Supported Cameras
  • Added Support for Spectra-Physics Insight Dual Port Laser
  • Added Support for Coherent Discovery Dual Port Laser
  • Added Support for Thorlabs' Tiberius Tunable TiSapphire Laser; Allows for Fast Sequence Capture
  • Added Support for Hyperspectral Imaging System; Allows Capture of a Hyperspectral Sequential Image Stack
  • Added Support for Spatial Light Modulator (SLM) Photo Activation; Allows for Calibration and User Interface for ROI Generated Patterns (Includes additional digital outputs for Galvo-Galvo)
  • Added Support for Thorlabs' Beam Stabilizer
  • Added Support for External USB National Instruments cards (USB-6363 and USB-6341)
  • Added Support for two additional Digital Shutters (Configured similarly as previous ThorShutterDig)
New Features
  • Added Disconnect State for available devices not to be controlled by ThorImageLS
  • Added Multiple Modalities; Allows user to configure and switch between different Imaging Modalities Easily
  • Added Digital Offset capability for PMT2000; Adjustment allows for same dynamic Range between Galvo-Resonant and Galvo-Galvo
  • Added Pockels Digital Output for Galvo-Resonant Scanner. (Similar to previous Galvo-Galvo functionality; Includes Output of Digital Line During Bleaching and Image Acquisition)
  • Added Pockels Digital Output to be Active during Image Acquisition for Galvo-Galvo (Previously only available during Bleaching)
  • Added Configurable Software Buttons to control NI Digitial PFI lines
  • Added Focus Tilt adjustment for Tile Capture; Allows User to Configure 3 Point Focus Plane to Be Used during Capture and Ensures Focus Across All Tiles.
  • Added Ability to Set the Pockels Power Mapping to Linear
  • Added Option to Turn Off Pockels Blanking a 0% for Galvo-Galvo
  • Added Pockels Phase Shift Setting to Galvo-Galvo (Previously only available for Galvo-Resonant)
  • Added Ability to Configure Min and Max Field Size for Scanners
  • Added PMT Selection for Simultaneous Bleach Imaging; Allows users to select PMT to be used for Simultaneous Bleach Imaging
  • Added Improved Hardware Triggering to Start Acquisition
  • Added Linearization Table for MCLS laser
  • Added Estimated Bleach duration for each Bleaching ROI; Located in Bleach Setup
User Interface (UI) Improvements
  • Added Quick Access Icon for Hardware Settings Window
  • Enhanced Displayed Device Feature; Allows User to Configure which Devices are Displayed in Hardware Setup
  • Added PMT Polarity Option to the UI Under the PMT More Panel
  • Added Search Capability when Editing Application and Device Settings
  • Added Display of Bergamo Rotation Value in Secondary Z Panel (Value saved in Experiment.xml)
  • Added Pockels Minimum Power Indicator on Pockels Slider; Gives indication if Pockels is Operating Below Recommended Minimum power
  • Added Ability to Control a Fourth Pockels for Galvo-Galvo Configurations
  • Added Custom Configuration of Pixel Density List
  • Added Center Bleach Scanner and Pockels Power Control to Bleach Panel
  • Added Do Not Show Message for Fiji Install Location Message
  • Enhanced ROI Calculations (Changed calculations may be slightly diffrent from previous versions, up to 3 decimal places.)
Fixed Bugs
  • Pockels Digital Output not Going Low for Single Point Bleach
  • Delete Experiment Raw file if Capture is Stopped Before First Trigger arrives
  • 3D View not Working Correctly in Image Review for Tiled Experiments
  • ROI Chart, Stats Table and Line Profile View Settings Get Deselected when Deleting all ROIs
  • Field Size Setting Issue with Galvo-Galvo when Using the Up and Down Zoom Buttons
  • Time Series Trigger First Acting like Trigger for Tiling Experiments
  • Streaming Capture Freezes when using Histogram Controls
  • Last Digit in Negative Z Limit Value Cut Off in Z Control Panel
  • Z Position Unit Incorrect (Displays mm instead of µm)
  • Incorrect High Voltage Range for PMT1002 (Can now be set to 1.1V)
  • Application Fault Switching to Capture Tab if Bleaching Power is Non-Whole Number.

Brochures and Mind Map

The buttons below link to PDFs of printable materials for Bergamo® II microscopes.

Bergamo II Brochure ThorImageLS Brochure Tiberius Brochure Download

Bergamo II Mind Map

Thorlabs' sales engineers and field service staff are based out of eight offices across four continents. We look forward to helping you determine the best imaging system to meet your specific experimental needs. Our customers are attempting to solve biology's most important problems; these endeavors require matching systems that drive industry standards for ease of use, reliability, and raw capability.

Thorlabs' worldwide network allows us to operate showrooms in a number of locations where you can see our systems in action. We welcome the opportunity for personal interaction during your visit! A demo can be scheduled at any of our showrooms by contacting ImagingSales@thorlabs.com.

Showroom Icon

Showroom and Customer Support Sites

 

Sterling, Virginia, USA
Thorlabs Imaging Systems HQ

Thorlabs Imaging Systems
108 Powers Court
Sterling, VA 20166

Appointment Scheduling and Customer Support

 

China

Thorlabs China
Room A101, No. 100, Lane 2891, South Qilianshan Road
Shanghai 200331

Appointment Scheduling and Customer Support

 

Japan

Thorlabs Japan, Inc.
Higashi-Ikebukuro Q Building, 2-23-2
Higashi-Ikebukuro, Toshima-ku, Tokyo 170-0013

Appointment Scheduling and Customer Support

Customer Support Sites

 

Newton, New Jersey, USA
Thorlabs HQ

Thorlabs, Inc.
56 Sparta Avenue
Newton, NJ 07860

Customer Support

 

United Kingdom

Thorlabs Ltd.
1 Saint Thomas Place, Ely
Ely CB7 4EX

Customer Support

 

Germany

Thorlabs GmbH
Hans-Boeckler-Str. 6
Dachau/Munich 85221

Customer Support

 

France

Thorlabs SAS
109, rue des Cotes
Maisons-Laffitte 78600

Customer Support

 

Brazil

Thorlabs Vendas de Fotônicos Ltda.
Rua Riachuelo, 171
São Carlos, SP 13560-110

Customer Support

Thorlabs recognizes that each imaging application has unique requirements.
If you have any feedback, questions, or need a quotation, please contact ImagingSales@thorlabs.com or call (703) 651-1700.

Selected Publications Using Thorlabs' Imaging Systems

2016

 

Mongeon R, Venkatachalam V, and Yellen G. "Cytosolic NADH-NAD+ Redox Visualized in Brain Slices by Two-Photon Fluorescence Lifetime Biosensor Imaging." Antioxid Redox Signal. 2016 Oct 1; 25 (10): 553-563.

Pachitariu M, Stringer C, Schröder S, Dipoppa M, Rossi LF, Carandini M, and Harris KD. "Suite2p: beyond 10,000 neurons with standard two-photon microscopy." bioRxiv. 2016 Jun 30; 061507.

Dipoppa M, Ranson A, Krumin M, Pachitariu M, Carandini M, and Harris KD. "Vision and locomotion shape the interactions between neuron types in mouse visual cortex." bioRxiv. 2016 Jun 11; 058396.

Rose T, Jaepel J, Hübener M, and Bonhoeffer T. "Cell-specific restoration of stimulus preference after monocular deprivation in the visual cortex." Science. 2016 Jun 10; 352 (6291): 1319–22.

Strobl MJ, Freeman D, Patel J, Poulsen R, Wendler CC, Rivkees SA, and Coleman JE. "Opposing Effects of Maternal Hypo- and Hyperthyroidism on the Stability of Thalamocortical Synapses in the Visual Cortex of Adult Offspring." Cereb Cortex. 2016 May 26; pii: bhw096 (epub ahead of print).

Lee KS, Huang X, and Fitzpatrick D. "Topology of ON and OFF inputs in visual cortex enables an invariant columnar architecture." Nature. 2016 May 5; 533 (7601): 90-4.

Monai H, Ohkura M, Tanaka M, Oe Y, Konno A, Hirai H, Mikoshiba K, Itohara S, Nakai J, Iwai Y, and Hirase H. "Calcium imaginq reveals glial involvement in transcranial direct current stimulation-induced plasticity in mouse brain." Nat Comm. 2016 Mar 22; 7 (11100): 1-10.

Ganmor E, Krumin M, Rossi LF, Carandini M, and Simoncelli EP. "Direct Estimation of Firing Rates from Calcium Imaging Data." arXiv. 2016 Jan 4; 1601.00364 (q-bio.NC): 1-34.

2015

 

Roth MM, Dahmen JC, Muir DR, Imhof F, Martini FJ, and Hofer SB. "Thalamic nuclei convey diverse contextual information to layer 1 of visual cortex." Nat Neurosci. 2015 Dec 21; 19 (2): 299-307.

Barnstedt O, Keating P, Weissenberger Y, King AJ, and Dahmen JC. "Functional Microarchitecture of the Mouse Dorsal Inferior Colliculus Revealed through In Vivo Two-Photon Calcium Imaging." J Neurosci. 2015 Aug 5; 35 (31): 10927-39.

Chen SX, Kim AN, Peters AJ, and Komiyama T. "Subtype-specific plasticity of inhibitory circuits in motor cortex during motor learning." Nat Neurosci. 2015 Jun 22; 18: 1109-15.

Jia Y, Zhang S, Miao L, Wang J, Jin Z, Gu B, Duan Z, Zhao Z, Ma S, Zhang W, and Li Z. "Activation of platelet protease-activated receptor-1 induces epithelial-mesenchymal transition and chemotaxis of colon cancer cell line SW620." Oncol Rep. 2015 Jun; 33 (6): 2681-8.

Lu W, Tang Y, Zhang Z, Zhang X, Yao Y, Fu C, Wang X, and Ma G. "Inhibiting the mobilization of Ly6Chigh monocytes after acute myocardial infarction enhances the efficiency of mesenchymal stromal cell transplantation and curbs myocardial remodeling." Am J Transl Res. 2015 Mar 15; 7 (3): 587-97.

Boyd AM, Kato HK, Komiyama T, and Isaacson JS. "Broadcasting of cortical activity to the olfactory bulb." Cell Rep. 2015 Feb 24; 10 (7): 1032-9.

Cossell L, Iacaruso MF, Muir DR, Houlton R, Sader EN, Ko H, Hofer SB, and Mrsic-Flogel TD. "Functional organization of excitatory synaptic strength in primary visual cortex." Nature. 2015 Feb 19; 518 (7539): 399-403.

2014

 

Partridge JG, Lewin AE, Yasko JR, and Vicini S. "Contrasting actions of group I metabotropic glutamate receptors in distinct mouse striatal neurones." J Physiol. 2014 Jul 1; 592 (Pt 13): 2721-33.

Peters AJ, Chen SX, Komiyama T. "Emergence of reproducible spatiotemporal activity during motor learning." Nature. 2014 Jun 12; 510 (7504): 263-7.

Ehmke T, Nitzsche TH, Knebl A, and Heisterkamp A. "Molecular orientation sensitive second harmonic microscopy by radially and azimuthally polarized light." Biomed Opt Express. 2014 Jun 12; 5 (7): 2231-46.

Liu J, Wu N, Ma L, Liu M, Liu G, Zhang Y, and Lin X. "Oleanolic acid suppresses aerobic glycolysis in cancer cells by switching pyruvate kinase type M isoforms." PLoS One. 2014 Mar 13; 9 (3): e91606.

Palmer LM, Shai AS, Reeve JE, Anderson HL, Paulsen O, and Larkum ME. "NMDA spikes enhance action potential generation during sensory input." Nat Neurosci. 2014 Feb 2; 17 (3): 383-90.

Cai F, Yu J, Qian J, Wang Y, Chen Z, Huang J, Ye Z, and He, S. "Use of tunable second-harmonic signal from KNbO3 nanoneedles to find optimal wavelength for deep-tissue imaging." Laser & Photon Rev. 2014; 8: 865-874.

2013

 

Kato HK, Gillet SN, Peters AJ, Isaacson JS, and Komiyama T. "Parvalbumin-expressing interneurons linearly control olfactory bulb output." Neuron. 2013 Dec 4; 80 (5): 1218-31.

Takata N, Nagai T, Ozawa K, Oe Y, Mikoshiba K, and Hirase H. "Cerebral blood flow modulation by Basal forebrain or whisker stimulation can occur independently of large cytosolic Ca2+ signaling in astrocytes." PLoS One. 2013 Jun 13; 8 (6): e66525.

Laser Scanning
Scan Path Wavelength Range 450 - 1100 nm, 680 - 1600 nm, or 900 - 1900 nm
Field of View 20 mm Diagonal Square (Max) at the Intermediate Image Plane
Scan Paths Galvo-Resonant Scanners, Galvo-Galvo Scanners, or Spatial Light Modulator
Single or Dual Scan Paths
Scan Speed 8 kHz Galvo-Resonant Scanner 2 fps at 4096 x 4096 Pixels
30 fps at 512 x 512 Pixels
400 fps at 512 x 32 Pixels
12 kHz Galvo-Resonant Scanner 45 fps at 512 x 512 Pixels
600 fps at 512 x 32 Pixels
Galvo-Galvo 3 fps at 512 x 512 Pixels
48 fps at 512 x 32 Pixels
70 fps at 32 x 32 Pixels
Pixel Dwell Time: 0.4 to 20 µs
Galvo-Galvo Scan Modes Imaging: Line, Polyline, Square, or Rectangle
Non-Imaging: Circle, Ellipse, Polygon, or Point
Field of View 20 mm Diagonal Square (Max) at the Intermediate Image Plane
[12 mm Diagonal Square (Max) for 12 kHz Scanner]
Scan Zoom 1X to 16X (Continuously Variable)
Scan Resolution Up to 2048 x 2048 Pixels (Bi-Directional) [Up to 1168 x 1168 Pixels for 12 kHz Scanner]
Up to 4096 x 4096 Pixels (Unidirectional) [Up to 2336 x 2336 Pixels for 12 kHz Scanner]
Compatible Objective Threadings M34 x 1.0, M32 x 0.75, M25 x 0.75, and RMS
Multiphoton Signal Detection
Epi-Detection Up to Four Ultrasensitive GaAsP PMTs, Cooled or Non-Cooled
Forward Direction Two Ultrasensitive GaAsP PMTs
Maximum of Four PMTs Controlled by the Software at a Given Time
Collection Optics 8°, 10°, or 14° Collection Angle
(Angles Quoted When Using an Objective with a 20 mm Entrance Pupil)
Easy-to-Exchange Emission Filters and Dichroic Mirrors
Confocal Imaging
Motorized Pinhole Wheel with 16 Round Pinholes from Ø25 µm to Ø2 mm
Two to Four Laser Lines (488 nm Standard; Other Options Range from 405 nm to 660 nm)
Standard Multialkali or High-Sensitivity GaAsP PMTs
Easy-to-Exchange Emission Filters and Dichroic Mirrors
Widefield Viewing
Manual or Motorized Switching Between Scanning and Widefield Modes
Illumination Provided via LED or Liquid Light Guide
C-Mount Threads for Scientific Cameras
Transmitted Light Imaging
Differential Interference Contrast (DIC) or Dodt Gradient Contrast
Widefield or Laser Scanned
Illumination Provided by Visible and/or NIR LEDs
Compatible with Air or Oil Immersion Condensers
Translation
X and Y 2" (50.8 mm) Total Travel; 0.5 µm Encoder Resolution
Z 1" (25.4 mm) Total Travel; 0.1 µm Encoder Resolution
Piezo Objective Scanner Open Loop: 600 µm ± 10% Travel Range; 1 nm Resolution
Closed Loop: 450 µm Travel Range; 3 nm Resolution
Microscope Base
(Rotating Bodies Only)
5" (127 mm) Total Travel; 1 µm Encoder Resolution
Rotation
(Rotating Bodies Only)
-5° to +95°, -50° to +50°, or -45° to +45° Around Objective Focus
0.1° Encoder Resolution

Thorlabs recognizes that each imaging application has unique requirements.
If you have any feedback, questions, or need a quotation, please use our
multiphoton microscopy contact form or call (703) 651-1700.


Posted Comments:
jfpena  (posted 2016-12-19 18:15:55.003)
I am looking for a cheap way to do confocal imaging in vivo. Is this Bergamo II Series Multiphoton Microscope my best option? Can you send me a quote?
tfrisch  (posted 2016-12-22 11:44:31.0)
Hello, thank you for contacting Thorlabs. A member of our Imaging Team will reach out to you directly to discuss this system and your application.
birech  (posted 2016-11-17 06:33:49.463)
I asked for a price quote for this product, Bergamo II Series Multiphoton Microscopes three days ago. I am working at the University of Nairobi in Kenya and would wish to order one. Regards, Birech
tfrisch  (posted 2016-11-17 06:56:23.0)
Hello, thank you for contacting Thorlabs. I have forwarded this request to our Imaging Sales Team. I apologize for the delay.

Based on your currency / country selection, your order will ship from Newton, New Jersey  
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Last Edited: Nov 08, 2013 Author: Dan Daranciang