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Bergamo® II Series Multiphoton Microscopes


Bergamo® II Series Multiphoton Microscopes


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The Bergamo® II Series is Thorlabs' platform for multiphoton microscopy. Following the principle that the microscope should conform to the specimen, rather than the other way around, we created a completely modular imaging platform that adapts to a wide range of experimental requirements.

Options at a Glance

Laser Scanning

  • 8 kHz and 12 kHz Galvo-Resonant Scanners for High-Speed Imaging
  • Galvo-Galvo Scanners for User-Defined ROI Shapes and Photostimulation Patterns
  • NEW! Spatial Light Modulator for Simultaneous Targeting
  • Super Broadband Scan Optics Optimized for:
    • Photoactivation / Uncaging
    • Two-Photon Imaging
    • NEW! Three-Photon Imaging
  • NEW! Co-Registered Confocal Imaging

Microscope Body

  • Rotating Bodies
    • 5" of Coarse Vertical Motion
    • -5° to +95° (or -50° to +50°) Rotation Around the Sample
    • 2" of Fine XY Motion
    • 1" of Fine Z Motion
    • X, Y, and Z Rotate with Objective
  • Upright Bodies
    • Fine Z or Fine XYZ Motion

Signal Detection

  • Up to Four Simultaneous Detection Channels
  • Thermoelectrically Cooled or Large-Angle Non-Cooled GaAsP PMTs
  • 8°, 10°, or 14° Collection Optics in Epi Direction (for Ø20 mm Entrance Pupil)
  • Mechanical Shutters Available for Photostimulation
  • Option for Two Forward-Direction
    Detection Channels
  • Easy-to-Exchange Magnetic Filter Holders

Transmitted Light Imaging

  • Dodt Gradient Contrast (Widefield and
    Laser Scanned)
  • DIC (Widefield and Laser Scanned)
  • Illumination Modules are Easily Removed for In Vivo Experiments
  • Visible and NIR LED Illumination
  • NEW! Available for Rotating and
    Upright Bodies
Sam Rubin
Sam Rubin
Imaging Systems General Manager

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Images Taken by Thorlabs' Microscopes

Rotating Microscopes: The Flexibility to Bring the Objective to the Region of Interest

Bergamo II Rotating Body
Click to Enlarge

Dual-scan-path systems, like the one shown above, rotate from -5° to +95°.
Single-scan-path systems can be factory configured for -5° to +95° or -50° to +50°.

Like the original Bergamo® microscope, the Bergamo® II is available with a five-axis motorized body (X, Y, Z, θ, and microscope base) that translates the entire optical path, rotates around the sample position, and creates a large volume underneath the objective for complex experimental apparatuses.

Bergamo II systems with rotation can be equipped with a -5° to +95° range (for single and dual scan path configurations) or a symmetric -50° to +50° range (for single scan path configurations only). Since the microscope moves the entire optical system, it is not necessary to reposition the sample, adjust the focus, or re-locate the region of interest after the objective is rotated. An integrated elevator coarsely moves the objective over a 5" (12.7 cm) range in the vertical direction, supporting experimental setups and apparatuses of varying sizes.

Motorizing the optical system not only simplifies experiments for the user but also helps ensure that the designed optical performance of your microscope and your objectives is achieved. Our articulating periscope, detailed in the Modules tab, is a key feature of our rotating design.

Five-Axis Motorization of Rotating Bergamo II Microscopes

 

Rotating Bergamo® II System Examples

Configuration B248
Rotating Premium In Vivo Microscope
Bergamo II Configuration B248
Configuration B221
Rotating Mid-Level In Vivo Microscope
Bergamo II Configuration B221
Configuration B209
Rotating Entry-Level In Vivo Microscope

Bergamo II Configuration B209

Thorlabs recognizes that each imaging application has unique requirements.
If you have any feedback, questions, or need a quotation, please use our
multiphoton microscopy contact form or call (703) 651-1700.

Upright Microscopes: In Vivo and Slice Rigs

Upright Bergamo® II microscopes offer one or three axes of motion and an industry-leading throat depth. Designed around Thorlabs' 95 mm construction rails, they offer stable long-term support and excellent vibrational damping. They offer a linear dovetail mounting surface that accepts our user-installable transmitted light illumination modules.

For slice imaging with brightfield illumination, Dodt gradient contrast, or DIC, simply attach the modules to the rail, using a balldriver to tighten a few setscrews in place. To free room underneath the objective for large in vivo specimens, loosen the setscrews and remove the modules. The installation and removal process takes fewer than 5 minutes.

Our transmitted light imaging modules are compatible with industry-standard condensers from Nikon.

BergamoII Non-Rotating Body
Click to Enlarge

Installing the Transmitted Light Modules Enables Dodt Contrast,
Laser-Scanned Dodt Contrast, or DIC Imaging
BergamoII Non-Rotating Body
Click to Enlarge

Removing the Transmitted Light Modules Makes Room
Underneath the Objective for Live Animal Imaging

 

Non-Rotating Bergamo® II System Examples

Configuration B232
Mid-Level In Vitro and In Vivo Microscope
with Z-Axis Motion and XY Platform
BergamoII Configuration B232
Configuration B206
Entry-Level In Vitro and In Vivo Microscope with Z-Axis Motion
BergamoII Configuration B206
Configuration B264
Premium In Vitro and In Vivo Microscope with XYZ Motion
BergamoII Configuration B264
Configuration B203
Entry-Level In Vitro Microscope with XYZ Motion
BergamoII Configuration B203

Thorlabs recognizes that each imaging application has unique requirements.
If you have any feedback, questions, or need a quotation, please use our
multiphoton microscopy contact form or call (703) 651-1700.

Laser Scanning, Widefield Imaging, and Transmitted Light Imaging
Scan Paths Galvo-Resonant
  • 8 kHz Scanner: Image at 2 fps (4096 x 4096 Pixels), 30 fps (512 x 512 Pixels), or
    400 fps (512 x 32 Pixels)
  • 12 kHz Scanner: Image at 45 fps (512 x 512 Pixels) or 600 fps (512 x 32 Pixels)
Galvo-Galvo
  • User-Defined Scan Geometries: Squares, Rectangles, Circles, Ellipses, Lines, and Polylines
  • Capture Weak Signals with Long Dwell Time Integration
  • Consistent Dwell Times Across Field of View
  • 48 fps at 512 x 32 Pixels and 70 fps at 32 x 32 Pixels
Spatial Light Modulator
  • Simultaneous Multi-Site Photostimulation using Holographic Beam Control
  • Entirely Controlled Through ThorImage®LS
  • Precise Control Along Z-Axis of Photoexcited Locations
Widefield Viewing
  • Thorlabs or Third-Party Scientific Cameras
    • Visualize Samples with Fluorescence or Reflected Light
    • Compatible with C-Mount-Threaded Scientific Cameras
  • Single- or Multi-Filter-Cube Epi-Illuminator
    • Locate Areas of Interest Without Unnecessary Laser Excitation
    • Thorlabs' LEDs Provide Bright Illumination
    • Industry-Standard Liquid Light Guides Illuminate Across the Visible Spectrum
Dodt Gradient Contrast and DIC Modules
  • User-Removable Modules Convert Microscope Between In Vivo and Slice Imaging
  • Camera or Laser Scanned
  • Dodt Gradient Contrast: View Features in Tissue Slices
  • DIC: View Features in Thinner, Transparent Samples
Optical Performance
Choice of Detectors
  • Choice of Signal Collection Angles to Accommodate Different Sample Depths
    • 8° (For Two PMTs)
    • 10° (For up to Four PMTs)
    • 14° (For Two PMTs)
    • Angles Quoted for an Objective with a Ø20 mm Entrance Pupil
  • Available With or Without Mechanical Shutters for Photoactivation Experiments
  • Option for Two Highly Sensitive Forward Fluorescence Detection Channels
Minimal Distance Between Objective and First Collecting Lens
  • Large Collection Angle for Multiphoton Fluorescence Emission
  • Increased Collection Efficiency
Periscopes
  • Maintain Optimal Laser Alignment and Optical Performance over Microscope's Entire Travel Range
Scan Paths Designed in House
  • Super Broadband Correction Range of 400 - 1100 nm, 680 - 1600 nm, or 900 - 1900 nm
  • Optimized for Photostimulation, Two-Photon Imaging, or Three-Photon Imaging
  • Specifically Designed to Match the Low-Magnification, High-NA Objectives Popularly Used in Multiphoton Microscopy
  • Take Full Advantage of the Latest Widely Tunable Ti:Sapphire and OPO Systems
  • Fill Up to a Ø20 mm Back Aperture Objective
  • Up to F.N. 20
Day-to-Day Usage
Several Software Packages
  • ThorImage®LS: Our Internally Developed, Open-Source Solution
  • Full SDK for LabVIEW and C++ Available Upon Request
  • Compatible with ScanImage
Touchscreen Controller
  • Touchscreen Shows Current Position of All Axes
  • Tap to Save and Retrieve Two Positions in Space
Easy-to-Reach Emission Filters and Dichroic Holders
  • Filters are Accessed from the Front of the Microscope and Take Less than 5 Minutes to Exchange
Input and Output Triggers
  • Use Electrical Signals to Synchronize All Your Equipment
  • Input Triggers can Start a Single Series or an Indefinite Series
  • Output Triggers can be Sent at the Beginning of a Frame or Line
  • High-Bandwidth Signal Integration with Electrophysiology
Large User-Adjustable Volume Underneath Objective
  • Accommodates Large Preps and Setups
  • Approach Angles Around Objective are Not Restricted
  • Rotating Bodies have 5" (12.7 cm) of Elevator Travel for Easily Adapting the Microscope for Differently Sized Experimental Setups
-5° to +95° or -50° to +50° Rotation of the Focal Plane (Rotating Bodies Only)
  • Image Different Sections of the Brain without Having to Move Your Specimen or Refocus the Objective
Beam Conditioning Modules
Pockels Cells
  • Edge and Fly-Back Blanking to Minimize Sample Photobleaching
  • High-Speed Masking for ROIs
  • Customize Laser Power at Each Slice Using Software Control
Variable Attenuator
  • Manual and Computer Control of Laser Power in Systems Without a Pockels Cell
  • Improves Pockels Cell Performance
  • One-Click Shutter
Variable Beam Expander
  • 1X - 3X Beam Diameter Modulation into the Objective Back Aperture Using Software Control
Beam Stabilizer
  • Maintain Stable Beam Pointing While Changing the Excitation Laser Wavelength
Sample Holders
Rigid Stands for Slides, Recording Chambers, or Platforms
  • Minimal Footprint Conserves Space Around Objective and the Microscope
  • Slim Profile Leaves Room for Dodt or DIC Imaging Modules
  • Excellent Long-Term Stability
  • Easily Rotate Samples Into and Out of the Beam Path
XY Platforms for Micromanipulators
  • Large Working Space that Surrounds the Objective on Three Sides
  • Ideal for Setups Where the Sample and Apparatus Need to Move in Unison, Such as Patch Clamping
  • 2" Travel in X and Y; 0.5 µm Encoder Resolution
Thorlabs Support
Fully Designed and Manufactured in House
  • Engineers Work Under One Roof to Lower Your Costs and Create Seamless Solutions
  • Expertise in Every System Component
Modular System Construction
  • As Your Experimental Needs Evolve, Upgrade Your Microscope Without Sacrificing Existing Capabilities
Professional Installation
  • Thorlabs Technician Visits Your Lab to Assemble, Test, and Demonstrate Use of Your Microscope
Quick Support
  • Communicate with Our Support Staff Faster than an Engineer Could Travel to Your Location
  • Thorlabs Technicians and Application Specialists Available for Videoconferencing
  • Thorlabs Will Ship You a Camera with a Microphone to Facilitate the Conversation
  • With Permission, Thorlabs Will Remote Desktop in to Address Software Issues

Thorlabs recognizes that each imaging application has unique requirements.
If you have any feedback, questions, or need a quotation, please use our
multiphoton microscopy contact form or call (703) 651-1700.

Bergamo® II Modules in Detail

BergamoII Rotating Body

Galvo-Resonant Scanners, Galvo-Galvo Scanners, and Spatial Light Modulators

Bergamo® II microscopes can be configured with a galvo-resonant scan path, a galvo-galvo scan path, and/or a spatial light modulator (SLM). These choices allow the user to optimize each experiment as needed for high frame rates, high sensitivity, and/or targeted exposure of the regions of interest.

Galvo-Resonant Scanners for High-Speed Imaging
Thorlabs offers 8 kHz and 12 kHz galvo-resonant scanners. Our 8 kHz scanners utilize the entire field of view and offer a maximum frame rate of 400 fps, while our 12 kHz scanners provide an increased frame rate of 600 fps.

Galvo-Galvo Scanners for User-Defined ROI Shapes
Galvo-galvo scanners support user-drawn scan geometries (lines, polylines, squares, and rectangles) and also support custom photoactivation patterns (circles, ellipses, polygons, and points). They offer consistent pixel dwell times for better signal integration and image uniformity.

Spatial Light Modulator for Simultaneous Targeting
Unlike scanners, which physically move from point to point, spatial light modulators (SLMs) use holography to direct the beam. This allows multiple sites in a sample to be photoexcited truly simultaneously.

Example Configurations with Dual Galvo-Resonant
and Galvo-Galvo Scan Paths
Configuration B264: Premium In Vitro and In Vivo Microscope with XYZ Motion
Configuration B248: Rotating Premium In Vivo Microscope
Example Configurations with a 
Galvo-Resonant Scan Path Only
Configuration B209: Rotating Entry-Level In Vivo Microscope
Configuration B221: Rotating Mid-Level In Vivo Microscope
Configuration B206: Entry-Level In Vitro and In Vivo Microscope with
Z-Axis Motion
Configuration B203: Entry-Level In Vitro Microscope with XYZ Motion
Example Configuration with a
Galvo-Galvo Scan Path Only
Configuration B232: Mid-Level In Vitro and In Vivo Microscope with Z-Axis Motion
and XY Platform

 

BergamoII Rotating Body
Click to Enlarge

Rotating Bergamo II systems are outfitted with multi-joint articulating periscopes. This periscope's design offers the enhanced flexibility needed to allow the entire scanning system to be tilted with respect to the sample.
BergamoII Non-Rotating Body
Click to Enlarge

Upright Bergamo II systems are equipped with periscopes that permit the microscope's full travel range in X, Y, and Z to be used without compromising the optical performance.

Periscopes

Most lasers used in multiphoton microscopy are delivered by a free-space beam. To stay aligned and optimized, the beam path must remain fixed. The Bergamo® II's ability to translate the objective around the focal plane in up to five axes (X, Y, Z, θ, and microscope base) also requires the beam path to translate along the same axes. Bergamo II systems overcome this engineering challenge using multi-jointed periscopes.

Example Configurations with Articulating Periscopes
Configuration B209: Rotating Entry-Level In Vivo Microscope
Configuration B221: Rotating Mid-Level In Vivo Microscope
Configuration B248: Rotating Premium In Vivo Microscope
Example Configurations with Fixed Periscopes
Configuration B206: Entry-Level In Vitro and In Vivo Microscope with
Z-Axis Motion
Configuration B232: Mid-Level In Vitro and In Vivo Microscope with
Z-Axis Motion and XY Platform
Configuration B203: Entry-Level In Vitro Microscope with XYZ Motion
Configuration B264: Premium In Vitro and In Vivo Microscope with XYZ Motion

 

BergamoII Filter Exchange
Click to Enlarge

The emission filters and dichroic cubes are held behind magnetically sealed doors on the front of the PMT detection module.

Super Broadband Scan Optics

Bergamo® II microscopes feature proprietary scan optics that are optimized and corrected for excitation wavelengths within the 400 - 1100 nm, 680 - 1600 nm, or 900 - 1900 nm wavelength range, ideal for photostimulation, two-photon imaging, and three-photon imaging, respectively. These broad ranges, extending from the visible well into the near infrared, were chosen to support the latest widely tunable Ti:Sapphire lasers and OPO systems, as well as dual-output lasers such as the Chameleon Discovery.

Our optics take full advantage of the optical designs used in the low-magnification, high-numerical-aperture objectives that have become the workhorses of multiphoton microscopy. They allow the back aperture of the objective to be filled up to Ø20 mm while providing a scan area that is 1.6X larger than that offered on our original Acerra and Bergamo multiphoton microscopes. This expanded area lets you find a region of interest more quickly or simply image more cells at once.

Detectors in Epi and Transmitted Directions

We employ high-sensitivity GaAsP PMTs in our multiphoton systems. These detectors offer high quantum efficiency, aiding in imaging weakly fluorescent or highly photosensitive samples. Two types are available: a thermoelectrically cooled PMT for improved sensitivity toward weak signals, and a non-cooled PMT that offers a smaller package size and greater numerical aperture.

All Bergamo® II microscopes can be equipped with two or four detection channels in the epi direction, and/or two detection channels in the forward direction. The user can configure the forward-direction channels to detect the same fluorescent tags as the epi-direction PMTs, raising the microscope's sensitivity toward thin, weakly fluorescent specimens.

A maximum of four channels can be controlled by the software at a given time.

Easy-to-Reach Emission Filters and Dichroic Holders

Bergamo® II systems are fully compatible with industry-standard fluorescence filter sets that include Ø25 mm fluorescence filters and 25 mm x 36 mm dichroic mirrors. Unlike competing designs, Thorlabs' detector modules have magnetic holders that make it simple and quick to exchange filters for different measurements.

We also offer detection modules for large-area Ø32 mm fluorescence filters and 32 mm x 42 mm dichroics, which support greater collection angles for increased signal.

 

Multi-Axis Controller with Touchscreen

This controller is specifically designed for rotating Bergamo® II microscope bodies. It uses knobs to control up to five motorized axes. On rotating systems, a rocker switch changes between fine objective focusing and translation of the elevator base. Each axis can be disabled on an individual basis in order to maintain a location along the desired direction.

The integrated touchscreen lets two spatial locations be saved and retrieved locally. Up to eight spatial locations can be saved on the computer running ThorImage®LS. The touchscreen also reads out the position of every motor.

 

Objectives

Bergamo® II microscopes accept infinity-corrected objectives with M34 x 1.0, M32 x 0.75, M25 x 0.75, or RMS threads. Together, these options encompass the majority of low-magnification, high-NA objectives used in multiphoton microscopy. With a large field number of 20, our scan optics completely utilize the optical designs of these specialized objectives, offering enhanced light-gathering ability compared to competing microscopes using the same objectives.

 

Rigid Stand Sample Holders

Thorlabs' Rigid Stands are rotatable, lockable, low-profile platforms for mounting slides, recording chambers, our Z-axis piezo stages, and custom experimental apparatuses. Each fixture is supported by a solid Ø1.5" stainless steel post for passive vibrational damping, which is in turn held to the workstation by the red post holder.

A locking collar maintains the height of the platform, allowing it to easily rotate into and out of the optical path, and a quick-release mechanism holds the post in place once the desired position is achieved.

 

BergamoII Rotating Body
Click to Enlarge

Four Epi-Direction PMTs with 10° Collection Optics and Mechanical Shutters

Large-Angle Signal Collection Optics

Deriving the most signal from limited photons is the fundamental goal of any detection system. To this end, we employ ultrasensitive GaAsP PMTs that offer high quantum efficiency. By positioning the PMTs immediately after the objective (a "non-descanned" geometry), light that is scattered by the sample, which therefore appears to originate outside the objective's field of view, still strikes the PMTs and adds to the collected signal. This is a benefit unique to multiphoton microscopy. Collecting beyond the objective's design field of view greatly enhances overall detection efficiency when imaging deep in tissue.

In the epi direction, we offer signal collection angles of 8°, 10°, or 14° (for a Ø20 mm entrance pupil), while in the transmtited direction, we offer signal collection angles of 13°. Our collection modules can optionally be outfitted with mechanical shutters for photoactivation experiments.

Example Configurations with Full-Field-of-View
Collection Optics
Configuration B203: Entry-Level In Vitro Microscope with XYZ Motion
Configuration B209: Rotating Entry-Level In Vivo Microscope
Example Configurations with Extended-Field-of-View
Collection Optics
Configuration B221: Rotating Mid-Level In Vivo Microscope
Configuration B248: Rotating Premium In Vivo Microscope
Configuration B206: Entry-Level In Vitro and In Vivo Microscope with
Z-Axis Motion
Configuration B232: Mid-Level In Vitro and In Vivo Microscope with Z-Axis Motion
and XY Platform
Configuration B264: Premium In Vitro and In Vivo Microscope with
XYZ Motion

 

Bergamo II with Dodt Contrast
Click to Enlarge
Dodt Contrast Module, Motorized Condenser Stage, and
Rigid Stand Sample Holder Underneath the Objective

User-Installable Dodt Contrast and DIC Imaging Modules

The modular construction of the Bergamo® II makes it exceptionally easy for the user to convert the microscope between in vitro and in vivo applications. Our user-installable Dodt contrast, laser-scanned Dodt contrast, and DIC modules take less than 5 minutes to attach or remove from the microscope body. These modules are available for both rotating and upright bodies.

Each option is paired with our basic 3-axis controller, which optimizes the illumination conditions by translating our motorized condenser stage over a 1" range. This versatile design is compatible with air and high-NA oil immersion condensers designed by Nikon.

To complement these modules, we manufacture slim-profile rigid stand sample holders that are ideal for positioning slides between the transmitted light module and the objective.

Example Configuration with Laser-Scanned Dodt Contrast
Configuration B264: Premium In Vitro and In Vivo Microscope with XYZ Motion

 

BergamoII Scientific Cameras
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1.4 Megapixel Scientific Camera

Scientific Cameras

Our low-noise, scientific-grade CCD cameras were designed for full compatibility with Thorlabs’ multiphoton microscopy systems. Useful for widefield and fluorescence microscopy, they are capable of visualizing in vitro and in vivo samples using reflected light and fluorescence emission. They work in conjunction with the epi-fluorescence module to help locate fiducial markers, and they also enable imaging modalities that do not require laser exposure.

Thorlabs' cameras are driven by our internally developed ThorCam software package, and are available in 1.4 megapixel, 4 megapixel, 8 megapixel, and fast-frame-rate versions. Generally speaking, cameras with lower resolution offer higher maximum frame rates. These cameras also feature a separate auxiliary port that permits the image acquisition to be driven by an external electrical trigger signal.

Bergamo® II microscopes are also directly compatible with any camera using industry-standard C-mount or CS-mount threads.

Thorlabs recognizes that each imaging application has unique requirements.
If you have any feedback, questions, or need a quotation, please use our
multiphoton microscopy contact form or call (703) 651-1700.

Configuration B209

Rotating Entry-Level In Vivo Microscope

BergamoII Rotating Body
Click to Enlarge

The built-in elevator on all rotating Bergamo II configurations helps create a large working volume underneath the objective. The image above shows the elevator at its maximum height.

With dual detection channels and 8° collection optics, this configuration is functionally equivalent to the original Bergamo® microscope. The single scan path is configured with Thorlabs' 8 kHz galvo-resonant scanner, which provides a video frame rate of 30 fps at 512 x 512 pixels and a maximum frame rate of 400 fps at 512 x 32 pixels.

Like all rotating Bergamo II microscopes, it is equipped with a scientific camera to visualize the field of view and locate fiducial markers. Widefield imaging is augmented by our single-cube epi-illuminator, which can be outfitted with one of Thorlabs' mounted LEDs or an industry-standard liquid light guide.

Selected Features Benefits
Galvo-Resonant Scan Path
  • Video Frame Rate of 30 FPS at 512 x 512 Pixels
  • Video Frame Rate of 400 FPS at 512 x 32 Pixels
Elevator with 5" (12.7 cm) of Coarse Vertical Adjustment
  • Easily Adapt the Microscope for Differently Sized Experimental Setups
Rotation About the Focus
  • Image Different Sections of the Brain Without Having to Move Your Specimen or Refocus the Objective
  •  -5° to +95° or -50° to +50° Range
Easy-to-Reach Emission Filters and Dichroic Holders
  • Filters are Accessed from the Front of the Microscope and Take Less than 5 Minutes to Exchange
BergamoII Rotating Body

Thorlabs recognizes that each imaging application has unique requirements.
If you have any feedback, questions, or need a quotation, please use our
multiphoton microscopy contact form or call (703) 651-1700.

Configuration B221

Rotating Mid-Level In Vivo Microscope

360° Video of Configuration B221

In order to image more deeply in vivo than is possible with Bergamo® II Configuration B209, we upgraded this system to include 10° collection optics. Compared to our 8° collection optics, these optics provide a larger collection angle, improving the detection efficiency of signals that are scattered by thick samples. They also increase the signal-to-noise at layers closer to the surface for higher contrast.

This Bergamo II model has also been upgraded with a motorized, software-controlled dichroic changer that makes it possible to switch between laser scanning and widefield imaging modalities from the computer. In widefield imaging mode, light from the sample is captured by a scientific camera. The sample can optionally be illuminated using our epi-illuminator module, which can be outfitted with any of Thorlabs' LEDs or an industry-standard liquid light guide.

Selected Features Benefits
10° Collection Optics
  • Enhanced Collection of Photons Scattered by Thick Tissue
  • Improved Signal-to-Noise When Imaging Deep In Vivo
Motorized Dichroic Changer
  • Switch Between Widefield Imaging and Laser Scanning without Touching the Microscope
Rotation About the Focus
  • Image Different Sections of the Brain Without Having to Move Your Specimen or Refocus the Objective
  • -5° to +95° or -50° to +50° Range
Thorlabs' Epi-Illuminator with LED
  • Locate Fiducial Markers without Laser Exposure
  • Bright, Single-Color Illumination

BergamoII Non-Rotating Body

Thorlabs recognizes that each imaging application has unique requirements.
If you have any feedback, questions, or need a quotation, please use our
multiphoton microscopy contact form or call (703) 651-1700.

Configuration B248

Rotating Premium In Vivo Microscope

BergamoII Rotating Body
Click to Enlarge

The entire optical path is rotated along with the objective, allowing the designed performance of the objective to be achieved regardless of angle. 

This model is our most capable and versatile Bergamo® II microscope with rotation. In order to enable targeted photostimulation, we added a secondary galvo-galvo scan path that complements the primary galvo-resonant path. The separately controlled secondary beam stimulates a specific region within the field of view, then the galvo-resonant scan path automatically performs fast functional imaging at up to 400 fps (depending on the pixel dimensions of the desired image).

The galvo-galvo scan path may also be used independently from the galvo-resonant scan path. Galvo-galvo scanners can be targeted at specific regions within the field of view to minimize photo exposure to non-targeted areas. They also permit long pixel dwell times (range: 0.4 to 20 µs), helping to capture weak signals through integration.

Selected Features Benefits
Primary Galvo-Resonant Scan Path
  • Perform Functional Imaging in Concert with the Galvo-Galvo Scan Path
  • Video Frame Rate of 30 fps at 512 x 512 Pixels
  • Video Frame Rate of 400 fps at 512 x 32 Pixels
Secondary Galvo-Galvo Scan Path
  • Independently Positionable Beam for Targeted Photoactivation/Uncaging
  • Prevent Overexposure by Illuminating Only Selected Areas
  • Arbitrary Line, Square, and Rectangle Scanning Geometries
Four Detection Channels
  • Detect Signals from Four Fluorophores in the Same Scan
BergamoII Rotating Body

Thorlabs recognizes that each imaging application has unique requirements.
If you have any feedback, questions, or need a quotation, please use our
multiphoton microscopy contact form or call (703) 651-1700.

Configuration B206

Entry-Level In Vitro and In Vivo Microscope with Z-Axis Motion

BergamoII Rotating Body
Click to Enlarge

Upright Bergamo II systems can be equipped with our 3-axis knobbed controller, which provides variable translation speeds and switches that disable axes on an individual basis.

This Bergamo® II configuration is outfitted with two detection channels and 10° collection optics, offering high detection efficiency for both thin in vitro samples and thicker in vivo preparations. Compared to our 8° optics, these optics provide a larger collection angle that captures more of the signal photons that are scattered by thick tissue. These optics also boost the signal-to-noise at layers closer to the surface for increased contrast.

A motorized, software-controlled dichroic mover makes it possible to switch between laser scanning and widefield imaging modalities remotely from the computer interface.

Selected Features Benefits
Piezo Objective Holder
  • Perform High-Speed Z-Stack Imaging
Motorized Dichroic Changer
  • Switch Between Widefield Imaging and Laser Scanning without Touching the Microscope
Galvo-Resonant Scan Path
  • Video Frame Rate of 30 fps at 512 x 512 Pixels
  • Video Frame Rate of 400 fps at 512 x 32 Pixels
Easy-to-Reach Emission Filters and Dichroic Holders
  • Filters are Accessed from the Front of the Microscope
  • Filter Exchange Takes Less than 5 Minutes
Bergamo II Non-Rotating Body

Thorlabs recognizes that each imaging application has unique requirements.
If you have any feedback, questions, or need a quotation, please use our
multiphoton microscopy contact form or call (703) 651-1700.

Configuration B232

Mid-Level In Vitro and In Vivo Microscope with Z-Axis Motion and XY Platform

360° Video of Configuration B232

To capitalize on our 10° collection optics' design, we engineered a detection module capable of detecting four fluorophores simultaneously. The 4-channel detection module offers the same easy access to optics as the 2-channel version, making it simple to exchange emission filters and dichroics so that the microscope can detect different fluorescent tags.

To supplement the 1" of Z travel provided by the microscope body, our XY platform has been incorporated into this system. By recessing the 95 mm construction rail that serves as the microscope’s support structure, we are able to provide access to the objective from the front, sides, and even behind.

Selected Features Benefits
Four Detection Channels
  • Detect Signals from Four Fluorophores in the Same Scan
Galvo-Galvo Scan Path
  • Arbitrary User-Defined Line, Square, or Rectangle Scan Geometries
  • Longer Dwell Times Help Capture Weak Signals Through Integration
XY Platform Sample Holder
  • Move Complicated Experimental Setups as One
  • 2" Travel in X and Y
10° Collection Optics
  • Enhanced Collection of Photons Scattered by Thick Tissue
  • Improved Signal-to-Noise When Imaging Deep In Vivo

BergamoII Non-Rotating Body

Thorlabs recognizes that each imaging application has unique requirements.
If you have any feedback, questions, or need a quotation, please use our
multiphoton microscopy contact form or call (703) 651-1700.

Configuration B203

Entry-Level In Vitro Microscope with XYZ Motion

360° Video of Configuration B203

This Bergamo® II system is equipped with three independent, high-resolution motors for hands-free positioning of the objective in X, Y, and Z (X and Y: 2" travel and 0.5 µm resolution; Z: 1" travel and 0.1 µm resolution). It is complemented by a sample holder with our Z-axis piezo stage, which accepts multiwell plates, multiple slides, petri dishes, or even a small breadboard. The piezo stage allows these accessories to be positioned beneath the objective with 25 nm resolution in Z.

This configuration is also equipped with a Nikon epi-illuminator for widefield imaging. Available on upright Bergamo II microscope bodies with 8° collection optics, this module uses an industry-standard liquid light guide for illumination and holds up to six dichroics at once.

Selected Features Benefits
Independent Motion in X, Y, and Z
  • Move the Field of View without Disturbing the Specimen
  • 2" Travel in X and Y with 0.5 µm Resolution
  • 1" Travel in Z with 0.1 µm Resolution
Rigid Stand with Slide Holder
  • Minimal Footprint Conserves Space Around Objective and the Microscope
  • Easily Rotated Into and Out of Beam Path
Fixed Periscope
  • Maintains Optimal Laser Alignment and Optical Performance over Microscope's Entire X, Y, and Z Travel Ranges

BergamoII Non-Rotating Body

Thorlabs recognizes that each imaging application has unique requirements.
If you have any feedback, questions, or need a quotation, please use our
multiphoton microscopy contact form or call (703) 651-1700.

Configuration B264

Premium In Vitro and In Vivo Microscope with XYZ Motion

360° Video of Configuration B264

This configuration incorporates four detection channels with our 10° collection optics for deep imaging in vivo and is equipped with dual galvo-resonant and galvo-galvo scan paths for high-frame-rate functional imaging, targeted laser exposure, and photostimulation/uncaging experiments. It is also outfitted with our laser-scanned Dodt contrast module and a Nikon condenser with 1" of motorized travel in Z, highlighting the versatility of our modular microscope construction.

Our transmitted light modules can be installed or removed by the user on any upright Bergamo® II configuration in just a few minutes, making it exceptionally easy to switch between in vitro and in vivo imaging modalities. Thorlabs' rigid stand sample holders are the ideal match for systems that incorporate these transmitted light modules. Each has a narrow profile that allows slides to rotate easily into and out of the optical path.

Selected Features Benefits
Dodt Illumination Module
  • Perform Dodt Contrast, Laser Scanning, and Widefield Imaging with the Same Instrument
  • Easily Installed and Removed for In Vitro and In Vivo Experiments
Primary Galvo-Resonant and Secondary Galvo-Galvo
  • Combines Advantages of Galvo-Resonant and Galvo-Galvo Scanners in One Instrument
  • Use Galvo-Galvo Path to Photoactivate a Region of Interest, then Use Galvo-Resonant Path for High-Frame-Rate Functional Imaging
Fiber-Coupled Photostimulation Laser
  • Independently Optimize Wavelength in the Secondary Scan Path
  • Increase the Power Available in the Primary Scan Path

BergamoII Non-Rotating Body

Thorlabs recognizes that each imaging application has unique requirements.
If you have any feedback, questions, or need a quotation, please use our
multiphoton microscopy contact form or call (703) 651-1700.

ThorImage®LS Software

Comprehensive Imaging Platform for:

Seamless Integration with Experiments

  • Simultaneous Multi-Point Photoactivation and Imaging with Spatial Light Modulator
  • Fast Z Volume Acquisition with PFM450E or Third-Party Objective Scanners
  • Electrophysiology Signaling
  • Wavelength Switching with Tiberius® Laser or Coherent Chameleon Lasers
  • Pockels Cell ROI Masking
  • Power Ramped with Depth to Minimize Damage and Maximize Signal-to-Noise

Advanced Software Functionality

  • Multi-Column Customizable Workspace
  • Image Acquisition Synced with Hardware Inputs and Timing Events
  • Live Image Correction and ROI Analysis
  • Independent Galvo-Galvo and Galvo-Resonant Scan Areas and Geometries
  • Tiling for High-Resolution Large-Area Imaging
  • Independent Primary and Secondary Z-Axis Control for Fast Deep-Tissue Scans
  • Automated Image Capture with Scripts
    • Compatible with ImageJ Macros
  • Multi-User Settings Saved for Shared Workstations
  • Individual Colors for Detection Channels Enable Simple Visual Analysis
ThorImageLS Brochure

 

The full source code for ThorImage®LS is available for owners of a Bergamo, Cerna, or confocal microscope. E-mail us for your copy.

ThorImageLS is an open-source image acquisition program that controls Thorlabs' Bergamo II, confocal microscopes, and Cerna® with hyperspectral imaging, as well as supplementary external hardware. From prepared-slice multiphoton Z-stacks to simultaneous in vivo photoactivation and imaging, ThorImageLS provides an integrated, modular workspace tailored to the individual needs of the scientist. Its workflow-oriented interface supports single image, Z-stacks, time series, and image streaming acquisition, vizualization, and analysis. See the video at the top right for a real-time view of data acquisition and analysis with ThorImageLS.

ThorImageLS is included with a Thorlabs microscope purchase and open source, allowing full customization of software features and performance. ThorImageLS also includes Thorlabs’ customer support and regular software updates to continually meet the imaging demands of the scientific community.

 

New Functionality

Version 3.0 - November 2, 2016

Please contact ImagingTechSupport@thorlabs.com to obtain the latest ThorImageLS version compatible with your microscope. Because ThorImageLS 3.0 adds significant new features over 2.x and 1.x versions, it may not be compatible with older microscopes. We continue to support older software versions for customers with older hardware.

New Features

  • Added support for DDR05(/M) fast power control device, which allows for faster power ramping acquisitions
  • Added support for the entry level Galvo-Galvo Confocal system (no separate digital acquisition board necessary)
  • Added fine two-way calibration, which provides fewer two-way adjustments when changing field sizes
  • Added ability to save Galvo-Galvo offset and scale values to ThorConfocalGalvoSettings.xml file
  • Added option to save .tiff files compressed or uncompressed
  • Added option to save only enabled channels as raw image files
  • Added ability to save snapshot image either as a single image or as an experiment
  • Added ability for multi-location imaging on platforms with supporting stages (Bergamo II, MCM3000 and High-Speed Motorized XY Scanning Stages)
  • Includes new UI for defining and navigating multi-location regions
  • Added ability to invert scanners (flip horizontal and vertical) for Galvo-Resonant and Galvo-Galvo systems
  • Added ability to invert stage directions for Bergamo, MCM3000 and High-Speed Motorized XY Scanning Stages
  • Added ability to use an ROI mask (ROIMask.raw) as a Pockels mask. This includes updating the mask in the UI
  • Added ability to use Pockels power ramping during fast Z acquisitions
  • Added high temporal resolution image capture spacing. Allows the user to set a delay between frame acquisitions
  • Added ability to sequentially capture images using different hardware settings, such as Channel, PMT, Laser and Power
  • Added ability for High-Speed Motorized XY Scanning Stages laser calibration to linearize power output
  • Added support for a secondary Z panel
  • Added offset control support for PMT1000 and PMT2100 devices
  • Added ability to image and bleach simultaneously
  • Added option to save bleach images in Raw image format
  • Added ability to turn off the computer monitor when starting experiment capture
  • Added ability to synchronize the Start and Stop of ThorSync with ThorImage Capture start and stop
  • Added orthogonal view functionality for Z stack acquisitions in Image Review
  • Added new script commands to move X, Y and Z motors between script acquisitions
  • Added support for Tiberius two-photon laser

Fixed Bugs

  • Fixed bleaching capture fields from being editable during capture
  • Fixed raw image review not supporting 3D display
  • Fixed PMT3 and PMT4 voltage range setting saved in the template not being used when running a Script
  • Fixed galvo-galvo line scan setting the Y scale incorrectly when changing the X scale
  • Fixed error when galvo-galvo snapshot appears to lock up with large pixel density setting and dwell time, and added abort button
  • Fixed standard sensitivity PMTs not being set to 0 when the display indicates 0 after a hardware reset
  • A 4 channel snap-shot image is now saved as a multi-page image instead of RGB
  • Added an adjustable phase shift parameter to correct the pockels waveform sometimes being out of phase across the image 
  • Removed unnecessary files to correct for the application appearing locked up when selecting certain .xml files in the settings editor takes a long time

User Interface (UI) Improvements

  • Changed the layout for the hardware setup window
  • Added mouse scrolling functionality in settings editor
  • Removed un-necessary .xml files in settings editor view
  • Moved center scanners and resonance scanner from always visible to the area control advanced panel in Capture Setup
  • Added second column option for capture setup display
  • Moved field size entry to under scan area cartoon
  • Added dropdown list for most popular pixel density settings.
  • Added +/- buttons for Galvo-Galvo angle control
  • Changed the Z slider bar to objective graphical
  • Replaced coarse/fine buttons with buttons labeled increase/decrease
  • Changed Z units from mm to μm
  • Added visibility option for "Set Zero" feature in XY and Z panels
  • Added visibility for invert option in Z control panel
  • Added visibility option individual light path controls
  • Renamed the coherent control panel to multiphoton laser control
  • Display summary and status for collapsed panels in Capture Setup
  • Added features to histogram control:
    • Black and white point fields
    • Connector between black and white point fields to help locate mid-point
    • Ability to enlarge single or all histograms
    • Log scale display option
  • Stats chart and stats window changes:
    • Change how they are displayed. Selecting to close the window now de-selects visibility
    • Added ability to save a chart as a .jpg
    • Added ability to save table data as .csv, .txt or .raw
    • Changed the chart Y scale to scientific notation
    • Set the chart X axis limit to the range of data
  • Added display option for line profile window
  • Color settings changes:
    • Added more look up table (LUT) colors: BlueStat, CyanHot, GrayStat, GreenStat and RedStat
    • Allow the same LUT for multiple colors
    • Enhanced display of min and max when viewing the gray scale of single image features
  • Eliminated zoom-level edit dialog and replaced with user-entered zoom field
  • Changed experiment naming by adding a separate iteration field
  • Added dialog to suppress the "File Name Exists" prompt
  • Changed browsers to a more useful interface
  • Removed the intermediate menu when selecting image review
  • Added the ability to save more experiment information, such as Galvo-Galvo angle value and pinhole size
  • Z stack experiments open Z slider to mid-range
  • Added Z and T unit display for Z and time index
  • Image review play is now in a continuous loop until manually stopped
  • Changed Galvo-Galvo pixel dwell time scale bar to single bar

Brochures and Mind Map

The buttons below link to PDFs of printable materials for Bergamo® II microscopes.

Bergamo II Brochure ThorImageLS Brochure

Bergamo II Mind Map

Thorlabs' sales engineers and field service staff are based out of eight offices across four continents. We look forward to helping you determine the best imaging system to meet your specific experimental needs. Our customers are attempting to solve biology's most important problems; these endeavors require matching systems that drive industry standards for ease of use, reliability, and raw capability.

Thorlabs' worldwide network allows us to operate showrooms in a number of locations where you can see our systems in action. We welcome the opportunity for personal interaction during your visit! A demo can be scheduled at any of our showrooms by contacting ImagingSales@thorlabs.com.

Showroom Icon

Showroom and Customer Support Sites

 

Sterling, Virginia, USA
Thorlabs Imaging Systems HQ

Thorlabs Imaging Systems
108 Powers Court
Sterling, VA 20166

Appointment Scheduling and Customer Support

 

China

Thorlabs China
Room A101, No. 100, Lane 2891, South Qilianshan Road
Shanghai 200331

Appointment Scheduling and Customer Support

 

Japan

Thorlabs Japan, Inc.
Higashi-Ikebukuro Q Building, 2-23-2
Higashi-Ikebukuro, Toshima-ku, Tokyo 170-0013

Appointment Scheduling and Customer Support

Customer Support Sites

 

Newton, New Jersey, USA
Thorlabs HQ

Thorlabs, Inc.
56 Sparta Avenue
Newton, NJ 07860

Customer Support

 

United Kingdom

Thorlabs Ltd.
1 Saint Thomas Place, Ely
Ely CB7 4EX

Customer Support

 

Germany

Thorlabs GmbH
Hans-Boeckler-Str. 6
Dachau/Munich 85221

Customer Support

 

France

Thorlabs SAS
109, rue des Cotes
Maisons-Laffitte 78600

Customer Support

 

Brazil

Thorlabs Vendas de Fotônicos Ltda.
Rua Riachuelo, 171
São Carlos, SP 13560-110

Customer Support

Thorlabs recognizes that each imaging application has unique requirements.
If you have any feedback, questions, or need a quotation, please contact ImagingSales@thorlabs.com or call (703) 651-1700.

Selected Publications Using Thorlabs' Imaging Systems

2016

 

Mongeon R, Venkatachalam V, and Yellen G. "Cytosolic NADH-NAD+ Redox Visualized in Brain Slices by Two-Photon Fluorescence Lifetime Biosensor Imaging." Antioxid Redox Signal. 2016 Oct 1; 25 (10): 553-563.

Pachitariu M, Stringer C, Schröder S, Dipoppa M, Rossi LF, Carandini M, and Harris KD. "Suite2p: beyond 10,000 neurons with standard two-photon microscopy." bioRxiv. 2016 Jun 30; 061507.

Dipoppa M, Ranson A, Krumin M, Pachitariu M, Carandini M, and Harris KD. "Vision and locomotion shape the interactions between neuron types in mouse visual cortex." bioRxiv. 2016 Jun 11; 058396.

Rose T, Jaepel J, Hübener M, and Bonhoeffer T. "Cell-specific restoration of stimulus preference after monocular deprivation in the visual cortex." Science. 2016 Jun 10; 352 (6291): 1319–22.

Strobl MJ, Freeman D, Patel J, Poulsen R, Wendler CC, Rivkees SA, and Coleman JE. "Opposing Effects of Maternal Hypo- and Hyperthyroidism on the Stability of Thalamocortical Synapses in the Visual Cortex of Adult Offspring." Cereb Cortex. 2016 May 26; pii: bhw096 (epub ahead of print).

Lee KS, Huang X, and Fitzpatrick D. "Topology of ON and OFF inputs in visual cortex enables an invariant columnar architecture." Nature. 2016 May 5; 533 (7601): 90-4.

Monai H, Ohkura M, Tanaka M, Oe Y, Konno A, Hirai H, Mikoshiba K, Itohara S, Nakai J, Iwai Y, and Hirase H. "Calcium imaginq reveals glial involvement in transcranial direct current stimulation-induced plasticity in mouse brain." Nat Comm. 2016 Mar 22; 7 (11100): 1-10.

Ganmor E, Krumin M, Rossi LF, Carandini M, and Simoncelli EP. "Direct Estimation of Firing Rates from Calcium Imaging Data." arXiv. 2016 Jan 4; 1601.00364 (q-bio.NC): 1-34.

2015

 

Roth MM, Dahmen JC, Muir DR, Imhof F, Martini FJ, and Hofer SB. "Thalamic nuclei convey diverse contextual information to layer 1 of visual cortex." Nat Neurosci. 2015 Dec 21; 19 (2): 299-307.

Barnstedt O, Keating P, Weissenberger Y, King AJ, and Dahmen JC. "Functional Microarchitecture of the Mouse Dorsal Inferior Colliculus Revealed through In Vivo Two-Photon Calcium Imaging." J Neurosci. 2015 Aug 5; 35 (31): 10927-39.

Chen SX, Kim AN, Peters AJ, and Komiyama T. "Subtype-specific plasticity of inhibitory circuits in motor cortex during motor learning." Nat Neurosci. 2015 Jun 22; 18: 1109-15.

Jia Y, Zhang S, Miao L, Wang J, Jin Z, Gu B, Duan Z, Zhao Z, Ma S, Zhang W, and Li Z. "Activation of platelet protease-activated receptor-1 induces epithelial-mesenchymal transition and chemotaxis of colon cancer cell line SW620." Oncol Rep. 2015 Jun; 33 (6): 2681-8.

Lu W, Tang Y, Zhang Z, Zhang X, Yao Y, Fu C, Wang X, and Ma G. "Inhibiting the mobilization of Ly6Chigh monocytes after acute myocardial infarction enhances the efficiency of mesenchymal stromal cell transplantation and curbs myocardial remodeling." Am J Transl Res. 2015 Mar 15; 7 (3): 587-97.

Boyd AM, Kato HK, Komiyama T, and Isaacson JS. "Broadcasting of cortical activity to the olfactory bulb." Cell Rep. 2015 Feb 24; 10 (7): 1032-9.

Cossell L, Iacaruso MF, Muir DR, Houlton R, Sader EN, Ko H, Hofer SB, and Mrsic-Flogel TD. "Functional organization of excitatory synaptic strength in primary visual cortex." Nature. 2015 Feb 19; 518 (7539): 399-403.

2014

 

Partridge JG, Lewin AE, Yasko JR, and Vicini S. "Contrasting actions of group I metabotropic glutamate receptors in distinct mouse striatal neurones." J Physiol. 2014 Jul 1; 592 (Pt 13): 2721-33.

Peters AJ, Chen SX, Komiyama T. "Emergence of reproducible spatiotemporal activity during motor learning." Nature. 2014 Jun 12; 510 (7504): 263-7.

Ehmke T, Nitzsche TH, Knebl A, and Heisterkamp A. "Molecular orientation sensitive second harmonic microscopy by radially and azimuthally polarized light." Biomed Opt Express. 2014 Jun 12; 5 (7): 2231-46.

Liu J, Wu N, Ma L, Liu M, Liu G, Zhang Y, and Lin X. "Oleanolic acid suppresses aerobic glycolysis in cancer cells by switching pyruvate kinase type M isoforms." PLoS One. 2014 Mar 13; 9 (3): e91606.

Palmer LM, Shai AS, Reeve JE, Anderson HL, Paulsen O, and Larkum ME. "NMDA spikes enhance action potential generation during sensory input." Nat Neurosci. 2014 Feb 2; 17 (3): 383-90.

Cai F, Yu J, Qian J, Wang Y, Chen Z, Huang J, Ye Z, and He, S. "Use of tunable second-harmonic signal from KNbO3 nanoneedles to find optimal wavelength for deep-tissue imaging." Laser & Photon Rev. 2014; 8: 865-874.

2013

 

Kato HK, Gillet SN, Peters AJ, Isaacson JS, and Komiyama T. "Parvalbumin-expressing interneurons linearly control olfactory bulb output." Neuron. 2013 Dec 4; 80 (5): 1218-31.

Takata N, Nagai T, Ozawa K, Oe Y, Mikoshiba K, and Hirase H. "Cerebral blood flow modulation by Basal forebrain or whisker stimulation can occur independently of large cytosolic Ca2+ signaling in astrocytes." PLoS One. 2013 Jun 13; 8 (6): e66525.

Laser Scanning
Scan Path Wavelength Range 400 - 1100 nm, 680 - 1600 nm, or 900 - 1900 nm
Field of View 20 mm Diagonal Square (Max) at the Intermediate Image Plane
Scan Paths Galvo-Resonant Scanners, Galvo-Galvo Scanners, or Spatial Light Modulator
Single or Dual Scan Paths
Scan Speed 8 kHz Galvo-Resonant Scanner 2 fps at 4096 x 4096 Pixels
30 fps at 512 x 512 Pixels
400 fps at 512 x 32 Pixels
12 kHz Galvo-Resonant Scanner 45 fps at 512 x 512 Pixels
600 fps at 512 x 32 Pixels
Galvo-Galvo 3 fps at 512 x 512 Pixels
48 fps at 512 x 32 Pixels
70 fps at 32 x 32 Pixels
Pixel Dwell Time: 0.4 to 20 µs
Galvo-Galvo Scan Modes Imaging: Line, Polyline, Square, or Rectangle
Non-Imaging: Circle, Ellipse, Polygon, or Point
Field of View 20 mm Diagonal Square (Max) at the Intermediate Image Plane
[12 mm Diagonal Square (Max) for 12 kHz Scanner]
Scan Zoom 1X to 16X (Continuously Variable)
Scan Resolution Up to 2048 x 2048 Pixels (Bi-Directional) [Up to 1168 x 1168 Pixels for 12 kHz Scanner]
Up to 4096 x 4096 Pixels (Unidirectional) [Up to 2336 x 2336 Pixels for 12 kHz Scanner]
Compatible Objective Threadings M34 x 1.0, M32 x 0.75, M25 x 0.75, and RMS
Multiphoton Signal Detection
Epi-Detection Up to Four Ultrasensitive GaAsP PMTs, Cooled or Non-Cooled
Forward Direction Two Ultrasensitive GaAsP PMTs
Maximum of Four PMTs Controlled by the Software at a Given Time
Collection Optics 8°, 10°, or 14° Collection Angle
(Angles Quoted When Using an Objective with a 20 mm Entrance Pupil)
Easy-to-Exchange Emission Filters and Dichroic Mirrors
Confocal Imaging
Motorized Pinhole Wheel with 16 Round Pinholes from Ø25 µm to Ø2 mm
Two to Four Laser Lines (488 nm Standard; Other Options Range from 405 nm to 660 nm)
Standard Multialkali or High-Sensitivity GaAsP PMTs
Easy-to-Exchange Emission Filters and Dichroic Mirrors
Widefield Viewing
Manual or Motorized Switching Between Scanning and Widefield Modes
Illumination Provided via LED or Liquid Light Guide
C-Mount Threads for Scientific Cameras
Transmitted Light Imaging
Differential Interference Contrast (DIC) or Dodt Gradient Contrast
Widefield or Laser Scanned
Illumination Provided by Visible and/or NIR LEDs
Compatible with Air or Oil Immersion Condensers
Translation
X and Y 2" (50.8 mm) Total Travel; 0.5 µm Encoder Resolution
Z 1" (25.4 mm) Total Travel; 0.1 µm Encoder Resolution
Piezo Objective Scanner Open Loop: 600 µm ± 10% Travel Range; 1 nm Resolution
Closed Loop: 450 µm Travel Range; 3 nm Resolution
Microscope Base
(Rotating Bodies Only)
5" (127 mm) Total Travel; 1 µm Encoder Resolution
Rotation
(Rotating Bodies Only)
-5° to +95° or -50° to +50° Around Objective Focus
0.1° Encoder Resolution

Thorlabs recognizes that each imaging application has unique requirements.
If you have any feedback, questions, or need a quotation, please use our
multiphoton microscopy contact form or call (703) 651-1700.


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Posted Comments:
Poster:jfpena
Posted Date:2016-12-19 18:15:55.003
I am looking for a cheap way to do confocal imaging in vivo. Is this Bergamo II Series Multiphoton Microscope my best option? Can you send me a quote?
Poster:tfrisch
Posted Date:2016-12-22 11:44:31.0
Hello, thank you for contacting Thorlabs. A member of our Imaging Team will reach out to you directly to discuss this system and your application.
Poster:birech
Posted Date:2016-11-17 06:33:49.463
I asked for a price quote for this product, Bergamo II Series Multiphoton Microscopes three days ago. I am working at the University of Nairobi in Kenya and would wish to order one. Regards, Birech
Poster:tfrisch
Posted Date:2016-11-17 06:56:23.0
Hello, thank you for contacting Thorlabs. I have forwarded this request to our Imaging Sales Team. I apologize for the delay.

Based on your currency / country selection, your order will ship from Newton, New Jersey  
+1 Qty Docs Part Number - Universal Price Available / Ships
BERGAMO Support Documentation
BERGAMOMultiphoton Imaging Microscope
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Last Edited: Nov 08, 2013 Author: Dan Daranciang