Cerna® Birefringence Imaging Microscopes


  • Stress- and Strain-Induced Birefringence Imaging
  • Raw Measurement Mode with Multiple Input Polarizations
  • Measurements of Retardance and Azimuth (Fast Axis Orientation)

CM501

Cerna Birefringence Imaging Microscope with Manual Objective Arm

The optical table, sample holder,
and laptop are sold separately.

Sample Measurement Results Using the CM50x Software

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Side View of CM502 Microscope
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Thorlabs Imaging Systems

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CM50x Configuration Comparisona
Item # CM502 CM501
Included Motion Controller MCM3001 N/A
Included Objective Arm
Item # ZFM2020b ZFM1020b
Type Motorized Manual
Travel Range 1" (25.4 mm)
Resolution Bidirectional Repeatability: 1 μm 
Min. Incremental Movement: 100 nm 
~ 2 mm / 30°
Recommended Objectivesc N4X-PF, N10X-PF (Included), or N20X-PF N4X-PF or N10X-PF (Included)
  • For full specifications, please see the Specs tab above.
  • For full specifications on the ZFM1020 Manual Condenser Focusing Module and ZFM2020 Motorized Module, please see the full web presentation.
  • Since high NA optics contribute residual birefringence and might impact the system accuracy, we only recommend these objectives whose accuracy we have verified. 

Features

  • Measures Sample Retardance up to a Half-Wave at 633 nm (up to 316 nm Retardance) and Azimuths (Fast Axis Orientation) up to ±90°
  • Spatial Resolution of 1.055 μm with Included 10X, 0.3 NA Objective
    • Additional Verified Accurate Objectives Sold Below
  • Built-In 633 nm Light Source
  • Two Configurations Available
    • Motorized Objective Arm (Microscope Item # CM502)
    • Manual Objective Arm (Microscope Item # CM501)  
  • Custom Operating Wavelengths Available by Contacting Tech Support

Applications

  • Stress-Induced Birefringence Imaging
  • Birefringence Sensitive Tissue Analysis
  • Dye-Free, Label-Free Imaging
  • Structural Imaging
  • Pathologic Diagnosis
  • Material Research
  • Quality Inspection

Thorlabs’ Cerna® Birefringence Imaging Microscopes are complete systems for measuring the retardance and azimuth of birefringent materials. With retardance measurements as low as ±1 nm, azimuth measurements as small as ±1°, spatial resolution of 1.055 μm with the included objective, and the flexible Cerna platform, these microscopes are a versatile solution for birefringence imaging. The CM50x microscopes are designed for use in academic research, medical diagnostics, industrial manufacturing, and product quality assurance. Since the operation is based on a liquid crystal device, there is no internal mechanical movement, leading to very stable, vibration-free operation. Please see the Technique tab above for details on operation and examples of application data taken with these microscopes. 

The illumination wavelength of the systems offered below is 633 nm. This wavelength can be customized from 488 nm to 780 nm by contacting Tech Support. The system includes a standard objective with 10X magnification and measures retardance up to a half-wave at 633 nm (up to 316 nm retardance) and azimuths up to ±90°. The full specifications of these microscopes are outlined in the Specs tab.

The microscope body is built on the flexible Cerna platform with 95 mm dovetail for easy integration with the wide range of compatible sample holders or custom modules that use our Cerna body attachments to integrate components from our large catalog of optomechanics. The CSN1202 Nosepiece incorporated into the CM50x microscopes can also hold two objectives. Please refer to the Microscope Add-Ons tab above for recommendations on sample holders and objectives whose accuracy has been verified for these birefringence measurements. 

The CM50x microscope consists of a main microscope body, an LED illumination module, 10X Nikon objective, scientific grade CMOS camera, and either a motorized (Microscope Item # CM502) or manual (Microscope Item # CM501) focusing module. See the table to the right for a comparison the two configurations. The Shipping List tab above provides details on what comes with each configuration.

A Windows®-based software package is included with the birefringence imaging microscope that contains every feature needed for system control and data acquisition. To view additional information about this system's software features and sample measurement results, please refer to the Software tab above.

These systems can be installed by the user and detailed instructions are provided in the CM50x manual. An overview of the assembly steps is shown in the Assembly tab above, and the Connections tab highlights all system connections. An optional installation service is available for customers at additional charge. Please contact Tech Support.

For applications requiring a larger field of view, our LCC7201B Birefringence Imaging System offers the same retardance and azimuth range and accuracy, but over a Ø20 mm field of view, and at a lower spatial resolution of 9.77 μm.

General Specifications
Item # CM502 CM501
Light Source Wavelengtha 632.8 nm ± 1 nmb
Microscope Cerna® Series Microscope
Measurement Range Retardance Standard Retardance Range: 0 nm to 316 nm
Low Retardance Range: 0 nm to 100 nm
Azimuth ±90°
Measurement Accuracy Retardance Standard Retardance Range: ±10 nm
Low Retardance Range: ±1 nm
Azimuth Standard Retardance Range: ±3°
Low Retardance Range: ±1°
Measurement Rate <20 s (Based on Default Camera Settings)
Field of Viewc 0.84 mm x 0.71 mm
Spatial Resolutionc 1.055 μm
Interfaces USB 2.0 and USB 3.0
Imager Resolution  2448 x 2048 Pixels (H x V)
Dimensions (D x W x H) 469.6 mm x 354.1 mm x 836.3 mm
(18.5" x 13.9" x 32.9")
Weight 22.4 kg (49.4 lbs) 21.9 kg (48.3 lbs)
Operating Temperature 0 °C to 40 °C
Storage Temperature -15 °C to 65 °C
  • Wavelength is customizable upon request. Contact Tech Support for details.
  • The M625L4 LED is used in combination with a FL632.8-1 filter to provide a 633 nm light source.
  • Field of view and spatial resolution are based on the standard configuration that includes a 10X objective (Item # N10X-PF).
Scientific Camera Specificationsa
Item # CS505MU
Sensor Type Monochrome CMOS
Number of Active Pixels 2448 x 2048 (H x V)
Pixel Size 3.45 μm x 3.45 μm
Imaging Area 8.4456 mm x 7.0656 mm (H x V)
Host PC Interface USB 3.0
Vertical Digital Binninga Continuous Integer Values from 1 to 16
Horizontal Digital Binninga Continuous Integer Values from 1 to 16
Exposure Time 0.021 ms to 7330 ms in ~0.007 ms Increments
Region of Interest (ROI) Frame Rate
Full Sensor (2448 x 2048) 53.2 fps
Half Sensor (1224 x 1024) 133.7 fps
~1/10th Sensor (260 x 208) 563.2 fps
Min ROI (260 x 4) 947.3 fps
  • For complete specifications, see the Specs tab on the main web presentation for our Kiralux® Compact Scientific Cameras.
Camera Port Specificationsa
Item # WFA4100
Magnification 1X
Dovetail Male D1N
Tube Lens Focal Length 200 mm
  • For complete specifications, see the full presentation for our Widefield Viewing Modules.
Objective Specificationsa
Item # N10X-PF
Magnification 10X
Numerical Aperture (NA) 0.3
Working Distance (WD) 16 mm
Parfocal Length 60 mm
Design Tube Lens Focal Length 200 mm
Coverslip Correction 0.17 mm
Barrel Diameter 30.0 mm
Length 48.7 mm
Threading M25 x 0.75
  • For complete specifications, see the Specs tab on the main web presentation for Imaging Microscope Objectives.

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Optical Schematic of CM50x Birefringent Microscope

Birefringence Imaging

Polarized Light Microscopy
Polarized Light Microscopy provides high contrast images of birefringent materials and can be used for both qualitative and quantitative measurements of the retardance and azimuth of these materials. Standard polarized light microscopy requires two rotatable, crossed polarizers, a white light source, and an adjustable phase compensator. The first polarizer sets the polarization of light entering the sample and the second polarizer acts as an analyzer. Measurements of the retardance and the azimuth are accomplished by rotating the sample stage, adjusting the phase compensator, and recording the interference color. The quantitative retardance of the sample can be determined using a Michel-Levy color chart, and this path length difference can be converted to an index of refraction difference if the thickness of the sample is known. Conversely, if the index difference is known, this measurement can be used to determine thickness. Polarized light microscopy relies on multiple steps and can be time-consuming due to the need for a color camera, sample rotation, and phase compensator adjustment.

CM50x Microscope Advantages and Operation
The CM50x Cerna® Birefringence Imaging Microscopes use polarimetry to provide quick and easy measurements of retardance and fast axis orientation of birefringent transparent materials without the need for sample rotation or a color camera. These measurements support research in fields such as industrial manufacturing, biology, and medicine. The microscope measures multiple intensity images recorded with various polarization states, which are precisely controlled by a liquid crystal module. Since there is no internal mechanical movement, these microscopes provide very stable, vibration-free operation.

A diagram of the system operation for the CM50x microscopes is shown at the right. An M625L4 Mounted LED in combination with a collimation lens and FL632.8-1 Filter provide a 633 nm collimated light source that is steered into the liquid crystal module by a WFA1000 Trans-Illumination Module. The liquid crystal module can set a number of different linear and circular input polarizations. The sample under test is placed at the focal plane of an included N10X-PF 10X objective (Nikon Plan Fluorite, 0.3 NA). Additional objectives with higher or lower NAs that have been verified to produce accurate birefringence measurements when used with the CM50x systems are also available. Please see recommendations in the Microscope Add-Ons tab above. A circular analyzer is set at the back focal plane of the objective to filter the transmitted light. A CS505MU Monochrome Scientific Camera records the intensity images for each sequential input state of polarization.

For details on system set up and general operation, please see the CM50x manual.

Applications
The CM50x Microscopes have applications in a variety of research and manufacturing fields. With the same retardance and azimuth range and accuracy as our LCC7201B Birefringence Imaging System, but higher spatial resolution of 1.055 μm with the included 10X objective compared to the 9.77 μm resolution of the LCC7201B system, these microscopes are a versatile solution to birefringence imaging.

Below, we show measurements of laser-induced birefringence in a polymer wave plate, stress-induced birefringence in an optical fiber, and several examples of birefringence in biological tissue. Click the tabs below for details. 

CM201 Shipping List
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CM502 Components

CM502 Cerna® Birefringence Imaging Microscope, Motorized Objective Arm

Item # CM502 consists of:

  • Main CM502 Microscope Body
  • Camera Assembly
  • LED Assembly
  • 10X Objective (Item # N10X-PF)
  • Birefringent NBS 1963A Resolution Target (Item # R2L2S1B)
  • 12 VDC Power Supply (Item # DS12)
  • Region-Specific Power Cord
  • USB Drive with Software and Manual (Not Shown)
  • USB 2.0 Type-A to Type-B Cable, 2 m
  • USB 3.0 A to Micro B Cable, 3 m*
  • Control Cable, 2 m
  • Extra Surface Protective Tapes for Storage or Shipping (Not Shown)
  • CCHK/M Metric Hex Key Set (Not Shown)
  • Three-Channel Controller and Knob Box (Item # MCM3001), Including
    • Knob Box
    • Controller Box
    • Joystick Controller Cable
    • Power Supply
    • Power Cord (Region-Specific)
    • USB Cable (A to B)
    • Two 1/4"-20 Cap Screws, 1/2" Long
    • Two M6 Cap Screws, 12 mm Long
    • Six 4-40 Hex Stand-Offs to Secure Cables

*The microscope performance specifications provided are guaranteed only with the provided USB 3.0 cable. 

 

CM100 Shipping List
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CM501 Components

CM501 Cerna Birefringence Imaging Microscope, Manual Objective Arm

Item # CM501 consists of:

  • Main CM501 Microscope Body
  • Camera Assembly
  • LED Assembly
  • 10X Objective (Item # N10X-PF)
  • Birefringent NBS 1963A Resolution Target (Item # R2L2S1B)
  • 12 VDC Power Supply (Item # DS12)
  • Region-Specific Power Cord
  • USB Drive with Software and Manual (Not Shown)
  • USB 2.0 Type-A to Type-B Cable, 2 m
  • USB 3.0 A to Micro B Cable, 3 m*
  • Control Cable, 2 m
  • Extra Surface Protective Tapes for Storage or Shipping (Not Shown)
  • CCHK/M Metric Hex Key Set (Not Shown)

*The microscope performance specifications provided are guaranteed only with the provided USB 3.0 cable. 

Application-Optimized Cerna Microscopes

Developed in collaboration with our colleagues in the field, the Cerna microscopy platform is uniquely modular and flexible, making it adaptable to a wide range of demanding experimental requirements. If you would like to work with our application specialists, engineers, and sales team to design your own microscope, please email ImagingSales@thorlabs.com.

Selected Accessories

In order to image with the CM50x Birefringence Imaging Microscopes, it is necessary to add a sample holder. To that end, we have ensured that Cerna® microscopes are compatible with a wide range of accessories. The information below compares some suggested Cerna-compatible components. For a comprehensive list of sample holders, please see our Sample Holders for DIY Cerna Systems.

The CSN1202 Nosepiece incorporated into the CM50x microscopes can hold two objectives. The systems come with an N10X-PF objective. Additional verified compatible objectives are listed below. 

Content


Verified Objectives

Item # N4X-PF N10X-PF N20X-PF
Photo
(Click to Enlarge)
Objective Class Plan Fluorite
Magnificationa 4X 10X 20X
Numerical Aperture (NA) 0.13 0.3 0.50
Working Distance (WD) 17.2 mm 16 mm 2.1 mm
Parfocal Length 60 mm
Threading M25 x 0.75
  • When Used with the Design Tube Lens Focal Length of 200 mm
  • CM50x Imaging Systems Directly Accept M25 x 0.75-Threaded Objectives (Nikon)
  • Unverified Objectives Not Recommended

The CSN1202 nosepiece can hold two objectives with M25 x 0.75 threads, commonly used by Nikon objectives. One N10X-PF 10X, 0.3 NA objective is included with each CM50x microscope. Since high-NA optics contribute residual birefringence and might impact the system accuracy, we verified three widefield Nikon objectives, shown in the table to the right, to ensure that the system accuracy can still be guaranteed while switching the objectives. Unverified objectives may result in loss of measurement accuracy and are not recommended.

Please note that objectives with a magnification greater than 10X are not recommended for use with the CM501 Microscope with Manual Objective Arm, as the focusing resolution is not sufficient for these higher NA objectives.

Please Note: Unverified objectives may lead to inaccurate birefringence measurements due to residual birefringence of the objectives. For applications requiring objectives outside those recommended here, please contact Tech Support to discuss your imaging needs.



XY Stage on Fixed Arm
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CM502 Microscope with CSA1051 Adapter Arm, CSS2001 Manual XY Stage, and CS2000 Slide Holder
XY Stage on Fixed Arm
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MLS203-1 Stage with MLS203P2 Slide Holder on CSA1000 Fixed Arm

Sample Holders

Thorlabs offers highly configurable solutions for mounting your sample beneath the objective of the Cerna microscope. Rigid stands are available with multiple platform styles that can accept slides, petri dishes, recording chambers, micromanipulators, and custom inserts. The included collar makes them lockable at a height and angle chosen by the user. An example rigid stand is shown at the right integrated with the CM501 Microscope. We also offer translation stages for these rigid stands that provide motorized horizontal translation of the sample.

Our fixed arms attach directly to the dovetail that spans the height of the microscope body, allowing them to be positioned anywhere along the body height, putting the sample directly into the microscope's optical path, and taking advantage of the existing footprint of the scope. For a pre-configured motorized sample holder solution, use the CSA1000 Fixed Arm with the MLS203-1 Fast XY Scanning Stage (shown in the left-most image above). This stage is compatible with our MZS500-E Piezo-Driven Insert, which adds high-resolution Z-axis adjustments. The CSA1051 Adapter Arm, paired with the CSS2001 Olympus Manual Stage and CSS2000 Olympus Slide Holder, provides a manual XY solution, shown in the center image above. Alternatively, the CSA1001 and CSA1002 Fixed Arms are compatible with Thorlabs' extensive selection of optomechanical components, allowing custom sample holder configurations to be integrated with this microscope.

Several compatible options are outlined in the tables below. For a comprehensive list of options, please see our Sample Holders for DIY Cerna Systems.

Rigid Stands
Rigid Stand
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MP100-MLSH Insert Holder

Rigid Stand
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MP250-RCH1 Recording
Chamber Holder

  • Circular Hole Designed for Recording Chambers
  • Height Range: 298.1 - 510.9 mm
  • Other Heights Available
Rigid Stand
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MP200 Rigid Stand with Platform

  • 24 M6 x 1.0 Tapped Holes for Holding Micromanipulators or Other Equipment
  • Height Range: 248.1 - 410.1 mm
  • Other Heights Available
Fixed Arms
Fixed Arm
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CSA1000 Fixed Arm

Fixed Arm
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CSA1051 Adapter Arm

  • Compatible with CSS2001 Olympus U-SV Manual XY Stage 
Fixed Arm
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CSA1001 Fixed Arm

Fixed Arm
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CSA1002 Fixed Arm

These microscope systems can be installed by the user and detailed instructions are provided in each manual. An optional installation service is available for these systems at additional charge. Please contact Tech Support for details.

Microscope Assembly
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Step 1: Use both hands to transfer the main unit to the optical table using the holding points shown above. Always wear gloves when handling, setting up, and working with the system.
Microscope Assembly
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Step 2: Mount the main unit onto the optical table using at least four screws, i.e., at least one screw at each corner.
Microscope Assembly
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Step 3: Unscrew the two illumination module dust cover locking screws and remove the cover.
Microscope Assembly
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Step 4: Connect the LED assembly using the four cage plate rods. Tighten the four locking screws on the illumination module to secure the cage rods to the main microscope body. 
Microscope Assembly
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Step 5: Replace the illumination module dust cover and tighten the four locking screws in the CP33/M Cage Plate to secure the cage rods to the cage plate. 
Microscope Assembly
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Step 6: Remove the green protective tape on the objective holder and install the N10X-PF Objective into the CSN1202 Nosepiece.
Microscope Assembly
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Step 7: Loosen the locking screw on the front of the microscope, above the product label, with a 2 mm hex key and remove the dust cover on the camera port.
Microscope Assembly
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Step 8: Put the camera assembly on the camera port and secure the camera assembly with the locking screw.
Microscope Assembly
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Step 9: Final CM501 Microscope Assembly

CM50x Microscope Connections

Camera Back Panel
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CM50x Camera Connections
Microscope LCD Connection
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CM50x Control Box Connection
Microscope Connections
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CM50x Main Body Connections
Camera Connections
Callout Description
1 USB 3.0 Micro B Connector (to Computer)a
2 12 Pin Hirose Female Connector (Not Used)
3 Status LED
  • The microscope performance specifications (see the Specs tab) are guaranteed only with the provided USB 3.0 cable.
Control Box Connection
Callout Description
1 Control Cable Connection (to Main Body)
Microscope Main Body Connections
Callout Description
1 Control Cable Connection (to Control Box)
2 Service Switch (for Technical Support Only)
3 USB 2.0 Type B Connector (to Computer)
4 LED Connector
5 Power Supply Connector
6 Power Switch

 

CM502 Motorized Arm Controller Connections

Motorized Arm Controller Back
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MCM3001 Controller Back Panel
Motorized Arm Controller Front
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MCM3001 Controller Front Panel
MCM3001 Controller Back Panel
Callout Description
1 USB 2.0 Type B Port for Computer Connectiona
2 Power Supply Connector
  • Controller can optionally be driven with separate, downloadable software. Please see the Software tab on the full web presentation for details.
MCM3001 Controller Front Panel
Callout Description
1 Power Switch on Side
2 Micro-D 15 Pin Female Stage Connectorsa
3 MC3001 Joystick Connection
  • Please see the Pin Diagram tab on the full web presentation for pin details.

Software

Version 2.1.3

Click the button below to visit the Cerna Birefringence Imaging Microscope software page.

Software Download

The Cerna® Birefringence Imaging Microscopes include a Windows®-based software package with every feature needed for system control and data acquisition. Please click the Software button to the right to download the latest software for this system.

Features

  • Standard and Low Noise Modes for Retardance and Azimuth Measurements
  • Raw Sample Mode for Intensity Measurement
  • Selectable Rectangular or Circular Region of Interest (ROI)
  • Adjustable Dynamic Camera Settings (Gain, Exposure Time, Black Levels)
  • Overexposure Detection
  • Auto Adjustment of Light Source Level
  • 1D, 2D, and 3D Measurement Result Displays
  • Data Output in Binary, CSV, and Image (PNG and TIFF) Formats

The software includes three modes: Standard Mode and Low-Noise Mode, which are used to measure a sample's retardance and azimuth, and Raw Sample Mode, which is used to measure intensity with different input polarizations. Standard and Raw Sample Modes provide a higher measurement rate with a lower signal-to-noise ratio, while Low Noise Mode provides a lower measurement rate with a higher signal-to-noise ratio. An exposure time of 300 ms is set as the default, which allows a measurement to be finished within 20 seconds in Standard Mode and 4 minutes in Low Noise Mode.

The standard and low-noise modes provide two ranges of measurement accuracy: Standard Retardance Range and Low Retardance Range. Standard Retardance provides a measurement accuracy of ±10 nm for retardances in the 0 nm to 316 nm range and ±3° for azimuths over the entire measurement range. Low Retardance Range will improve the measurement accuracy to ±1 nm for retardances in the 0 nm to 100 nm range and ±1° for azimuths over the entire measurement range. The minimum exposure time is 0.021 ms, and the maximum exposure time is 7330 ms.

Below are screenshots from the included user interface, showing the different measurement display modes available. The Preview, 1D, 2D, and 3D views are all of a liquid crystal polymer zero-order half wave plate (Item # WPH10E-830).


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Azimuth and Retardance 1D View

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Camera Preview

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Retardance 3D View

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Retardance with Azimuth Vector 2D View

To schedule an in-person or virtual demo appointment, please email ImagingSales@thorlabs.com.

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Try Our Microscopes In Person or Virtually

Thorlabs' sales engineers and field service staff are based out of nine offices across four continents. We look forward to helping you determine the best imaging system to meet your specific experimental needs. Our customers are attempting to solve biology's most important problems; these endeavors require matching systems that drive industry standards for ease of use, reliability, and raw capability.

Thorlabs' worldwide network allows us to operate demo rooms in a number of locations where you can see our systems in action. We welcome the opportunity to work with you in person or virtually. A demo can be scheduled at any of our showrooms or virtually by contacting ImagingSales@thorlabs.com.

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Demo Rooms and Customer Support Sites
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Birefringence Imaging Microscope with Motorized Objective Arm


Click to Enlarge

CM502 Microscope with Motorized XY Stage and Sample Holder
  • Appropriate for Full Range of Verified Objectives (See Microscope Add-Ons Tab)
    • 10X Objective Included
    • 4X and 20X Verified Objectives Available Separately Below
  • Provides Motorized Focusing Adjustment over 1" Travel Range
  • Operated by Included MCM3001 3-Axis Controller 
    • Two Free Channels Can Be Used to Control User-Supplied PLS-X or PLS-XY Stages (Not Included)

The CM502 Birefringence Imaging Microscope with Motorized Objective Arm incorporates our ZFM2020 Motorized Focusing Module to provide 1" of fine, variable-speed travel along the Z axis for focusing the system. Some key specifications for this module are shown in the table below. The fine Z-axis control allows this microscope to be used with higher resolution objectives (not included). Since high NA optics contribute residual birefringence and might impact the system accuracy, we only recommend objectives whose accuracy we have verified. For a list of verified objectives, please see the Microscope Add-Ons tab above.

ZFM2020 Motorized Translation Stage Specificationsa
Travel Range 1" (25.4 mm)
Bidirectional Repeatability 1 µm
Backlash 1 µm
Minimum Achievable Incremental Movement 100 nm
Minimum Achievable Repeatable Movement 200 nm
Velocity (Max) 7 mm/s
Acceleration (Max) 11 mm/s2
Thread Screw Pitch 0.3 mm
Step Angle 1.8°
Limit Switches Hall Effect Sensors
Phase Current 0.49 A
Phase Resistance 5.1 Ω
Phase Inductance 1.5 mH
Insulation Resistance 20 MΩ
  • For full specifications on the ZFM2020 Manual Condenser Focusing Module, please see the full web presentation.

The MCM3001 Three-Channel Controller is included with the CM502 microscope for control of the motorized focusing module. Because the microscope only requires one channel of motion control, the MCM3001 controller can also be used to control additional motion control components (not included). For example, the PLS-XY Translation Stage can be incorporated with a rigid stand and sample holder, to provide a full XYZ motorized adjustment microscope system. The image at the right shows the CM502 system used with a PLS-XY stage, a rigid stand, and an MLS203P2 Slide Holder.

More examples of recommended sample holders are listed in the Microscope Add-Ons tab above. For a comprehensive list of options, please see our Sample Holders for DIY Cerna Systems.

Based on your currency / country selection, your order will ship from Newton, New Jersey  
+1 Qty Docs Part Number - Universal Price Available
CM502 Support Documentation
CM502NEW!Customer Inspired! Cerna Birefringence Imaging Microscope, Motorized Objective Arm
$45,900.00
Lead Time

Birefringence Imaging Microscope with Manual Objective Arm


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CM501 Microscope with Manual XY Stage and Sample Holder
  • Coarse Focusing Adjustment Appropriate for Lower Resolution Imaging
  • Appropriate for Use with 10X Objective (Included) or 4X Verified Objective (Sold Separately Below)
  • Provides Manual Focusing Adjustment over 1" Travel Range

The CM501 Birefringence Imaging Microscope with Manual Objective Arm incorporates our ZFM1020 Manual Condenser Focusing Module to provide 1" of coarse adjustment along the Z axis for focusing the objective. This focusing module is controlled manually with approximately 2 mm of Z movement per 30° of rotation of the manual knob. For full specifications on the ZFM1020 Manual Condenser Focusing Module, please see the full web presentation. The CM501 microscope is appropriate for wider field of view imaging with lower resolution. We recommend this microscope for use with the included 10X objective as well as lower magnifications, such as the N4X-PF Imaging Objective. 

Manual Focusing Module Specificationsa
Travel Range 1" (25.4 mm)
Distance per Degree Turn of Focus Knobb ~2 mm / 30°
  • For full specifications on the ZFM1020 Manual Condenser Focusing Module, please see the full web presentation
  • The angular distance between each groove on the focus knob is 30°.

A full microscope system requires a sample holder. An example configuration is shown at the right. The CM501 microscope is incorporated with an MLS203P2 Slide Holder on a rigid stand, mounted on XR25P and XR25C Linear Translation Stages, for full manual XYZ control. A manually controlled system provides a cost-effective, full birefringence imaging system for lower resolution applications. The addition of a motorized sample stage in Z can also be used to make the CM501 microscope appropriate for higher resolution applications. 

More examples of recommended sample holders and stages are listed in the Microscope Add-Ons tab above. For a comprehensive list of options, please see our Sample Holders for DIY Cerna Systems.

Based on your currency / country selection, your order will ship from Newton, New Jersey  
+1 Qty Docs Part Number - Universal Price Available
CM501 Support Documentation
CM501NEW!Customer Inspired! Cerna Birefringence Imaging Microscope, Manual Objective Arm
$42,000.00
Lead Time

Verified Nikon Plan Fluorite Objectives

XY Stage on Fixed Arm
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View Product List
Item #QtyDescription
CM5021Cerna Birefringence Imaging Microscope, Motorized Objective Arm
N4X-PF14X Nikon Plan Fluorite Imaging Objective, 0.13 NA, 17.2 mm WD
CSA10511Olympus Stage Adapter Arm for Cerna Microscope, Male D1Y Dovetail
CSS20011Manual Olympus XY Stage for Upright Microscope, Female D1Y Dovetail
CSS20001Manual Olympus XY Stage Slide Holder
CM502 Microscope with Additional 4X Objective and Manual XY Sample Stage
  • Infinity-Corrected Plan Fluorite Design
  • Verified for Use with CM50x Birefringence Imaging Microscopes
  • M25 x 0.75 Threading
  • Designed for a Tube Lens Focal Length of 200 mm
  • 60 mm Parfocal Length

The Nikon Plan Fluorite Objectives sold on this page provide 4X or 20X magnification. They are designed to have high transmission in the UV to NIR wavelength range and to produce flat images across the field of view. Objectives here have been verified to provide accurate results when used with the CM50x microscopes. These multi-purpose objectives can also be utilized for brightfield microscopy, fluorescence microscopy, and polarization-sensitive observations. The N20X-PF objective is also good for both darkfield and DIC microscopy.

Each objective is designed for use with a tube lens focal length of 200 mm and is compatible with our DIY Cerna® Systems. They use M25 x 0.75 threading; to use these objectives with a different thread standard, please see our microscope objective thread adapters.

These objectives are designed for use from -18 °C (0 °F) to 60 °C (140 °F) and are not recommended for use at extreme temperatures.

Item # Wavelength Range Ma WD EFL NA EPb Resolutionc PFL Cover Glass
Thickness
Objective
Threading
N4X-PF UV to NIR 4X 17.2 mm 50 mm 0.13 13 mm 2.6 µm 60 mm 0 - 0.17 mm M25 x 0.75;
3.6 mm Depth
N20X-PF 20X 2.1 mm 10 mm 0.50 10 mm 0.7 µm 0.17 mm M25 x 0.75;
5 mm Depth
  • When Used with a 200 mm Focal Length Tube Lens
  • Entrance pupil diameter (EP) is defined at the back aperture of the objective and calculated as EP=2*NA*EFL.
  • The theoretical Rayleigh Resolution is determined by 0.61*λ/NA at λ = 550 nm; see the Resolution tab for details.

M = Magnification
WD = Working Distance
EFL = Effective Focal Length
NA = Numerical Aperture

EP = Entrance Pupil Diameter
OFN = Optical Field Number
PFL = Parfocal Length

Based on your currency / country selection, your order will ship from Newton, New Jersey  
+1 Qty Docs Part Number - Universal Price Available
N20X-PF Support Documentation
N20X-PF20X Nikon Plan Fluorite Imaging Objective, 0.50 NA, 2.1 mm WD
$1,266.53
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N4X-PF Support Documentation
N4X-PF4X Nikon Plan Fluorite Imaging Objective, 0.13 NA, 17.2 mm WD
$628.91
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