Create an Account  |   Log In

View All »Matching Part Numbers


Your Shopping Cart is Empty
         

Super Apochromatic Microscope Objectives


  • Infinity-Corrected Dry Microscope Objectives
  • Super Apochromatic Axial Color Correction
  • 2X, 4X, or 10X Magnification

TL10X-2P

10X Super Apochromat Objective
for Two-Photon Microscopy

Application Idea

Mount three of our super apochromatic objectives on a CSN500 objective turret with a PLE351 parfocal length extender for the TL4X-SAP and an M32A2 thread adapter for the TL10X-2P.

TL2X-SAP

2X Super Apochromat Objective

Related Items


Please Wait
Objective Lens Selection Guide
Objectives
Super Apochromatic Microscope Objectives
Microscopy Objectives, Dry
Microscopy Objectives, Oil Immersion
Physiology Objectives, Water Dipping or Immersion
Long Working Distance Objectives
Reflective Microscopy Objectives
UV Microscopy Objectives
532 nm and 1064 nm Objectives
Scan Lenses and Tube Lenses
Scan Lenses
Infinity-Corrected Tube Lens
Mounted Condenser

Did You Know?

Multiple optical elements, including the microscope objective, tube lens, and eyepieces, together define the magnification of a system. See the Magnification & FOV tab to learn more.

Webpage Features
info icon Zemax blackbox files for the objectives on this page can be accessed by clicking this icon below.

Features

  • Infinity-Corrected Super Apochromatic Design
  • Diffraction-Limited Axial Color Performance over 400 - 750 nm
  • Antireflection (AR) Coatings:
    • 2X and 4X Objectives: 350 nm - 700 nm
    • 10X Objective: 400 nm - 1300 nm
  • Numerical Aperture (NA) up to 0.5
  • No Vignetting over Entire Field
  • Ideal for Brightfield, Fluorescence, Multiphoton, and Confocal Imaging Applications
  • Magnifications Specified for Use with a 200 mm Tube Lens

Thorlabs' super apochromatic microscope objectives are designed to provide diffraction-limited axial color performance and a flat field of focus in a variety of imaging modalities without introducing vignetting. The high numerical apertures (NA) of these objectives make them ideal for widefield imaging. The super apochromatic objective design provides axial color correction throughout the visible range. The optical elements are AR-coated for improved transmission. 2X and 4X objectives feature a coating for the 350 - 700 nm range, while the 10X objective is coated for 400 nm to 1300 nm, ideal for multiphoton imaging applications.

In addition to a 0.50 NA, our 10X objective features a long 7.77 mm working distance and tapered tip, providing space for experimental equipment to be inserted between the objective and the sample. A correction collar and optical thickness scale allow the objective focus to be adjusted for a variety of media that may be present between the objective and the sample, such as aqueous solutions and cover glasses (see the Correction Collar tab for details). Note that this objective is not suitable for water-dipping, water-immersion, or oil-immersion techniques. 

Each objective housing is engraved with the Item #, magnification, NA, wavelength range, and working distance. The housings have external M25 x 0.75 threading and are designed for a tube lens of focal length 200 mm. Each objective is shipped in an objective case comprised of a lid and container. The threading for each objective is given below; to use the objectives with a different thread standard, please see our microscope objective thread adapters

Mounting the objectives on this page together or in combination with other objectives may require parfocal length extenders, which allow users to match parfocal lengths and switch between objectives of different magnification mounted in a turret or nose piece without significant refocusing.

Item # TL2X-SAP TL4X-SAP TL10X-2P
Specifications
Magnificationa 2X 4X 10X
Numerical Aperture 0.10 0.20 0.5
AR Coating Range 350 - 700 nm 400 - 1300 nm
AR Coating Reflectance
Per Surface
Ravg < 0.5%
@ 0° AOI
Rabs < 2.5% (400 - 450 nm)
Rabs < 1.75% (450 - 1300 nm)
@ 0° - 25° AOI
Total Transmisison
(Click to Enlarge)
Icon
Raw Data
Icon
Raw Data
Icon
Raw Data
Axial Color Diffraction Limited over 400 - 750 nmb
Field of View Ø11 mm Ø5.5 mm Ø2.2 mm
Working Distancec 56.3 mm 17.0 mm 7.77 mm
Effective Focal Length 100 mm 50 mm 20 mm
Parfocal Lengthc 95.0 mm 60.0 mmd 95.0 mm
Diameterc 30.5 mm 43.2 mm
Lengthc 43.5 mm 46.4 mm 90.4 mm
Housing Threads M25 x 0.75 M32 x 0.75
Thread Depth 3.2 mm 3.6 mm 3.2 mm
Field Numbere 22
Design Tube Lens Focal Lengthf 200 mm
Cover Glass Thickness 0 - 5.0 mm 0 - 5.0 mm 0 - 2.6 mm
Suitable Microscopy Techniques
Confocal
Two-Photon - -
Brightfield
Darkfield
Dodt
DIC -
Fluorescence
  • Magnification is calculated for when these objectives are used with a 200 mm focal length tube lens.
  • For details on this specification, please see the Graphs tab.
  • These dimensions are defined in the drawing to the right.
  • To match the 95.0 mm parfocal length of the other two objectives on this page, the TL4X-SAP objective can be mounted with a PLE351 Parfocal Length Extender.
  • The field number definition can vary between manufacturers. The field number for these objectives represents performance over the full operating wavelength range with no vignetting over the entire field of view.
  • For information on compatibility between tube lenses and objectives, see the Magnification & FOV tab.

Dimensional Drawing

2X Objective Performance Graphs

Click here for raw data for all plots.


Click to Enlarge

Transmission of the TL2X-SAP Objective. The blue shaded region denotes the wavelength range of the AR coating.

Click to Enlarge

Axial color describes the shift in the image plane across the operating wavelength range of the 2X super apochromatic microscope objective. The pink shaded region denotes diffraction-limited performance.

Click to Enlarge

The Strehl Ratio is a quantitative measurement of optical image formation quality. The Strehl Ratio for the TL2X-SAP over its field of view is shown in the graph above.

Click to Enlarge

This graph shows the Strehl Ratio of the TL2X-SAP over the objective's full operating wavelength range.

4X Objective Performance Graphs

Click here for raw data for all plots.


Click to Enlarge

Transmission of the TL4X-SAP Objective. The blue shaded region denotes the wavelength range of the AR coating.

Click to Enlarge

Axial color describes the shift in the image plane across the operating wavelength range of the 4X super apochromatic microscope objective. The pink shaded region denotes diffraction-limited performance.

Click to Enlarge

The Strehl Ratio is a quantitative measurement of optical image formation quality. The Strehl Ratio for the TL4X-SAP over its field of view is shown in the graph above.

Click to Enlarge

This graph shows the Strehl Ratio of the TL4X-SAP over the objective's full operating wavelength range.

10X Objective Performance Graphs

Click here for raw data for all plots.


Click to Enlarge

Transmission of the TL10X-2P Objective. The blue shaded region denotes the wavelength range of the AR coating.

Click to Enlarge

Axial color describes the shift in the image plane across the operating wavelength range of the 10X super apochromatic microscope objective. The pink shaded region denotes diffraction-limited performance. Diffraction-limited performance can be shifted to the NIR with refocusing.

Click to Enlarge

The Strehl Ratio is a quantitative measurement of optical image formation quality. The Strehl Ratio for the TL10X-SAP over its field of view is shown in the graph above.

Click to Enlarge

This graph shows the Strehl Ratio of the TL10X-2P over the objective's visible performance band.

Click for Details
Above shows a diagram of a TL4X-SAP; Thorlabs maintains the labeling convention shown above for all of its super apochromatic microscope objectives.

Glossary of Terms

Magnification
The magnification of an objective is the lens tube focal length (L) divided by the objective's focal length (F):

M = L / F .

The total magnification of the system is the magnification of the objective multiplied by the magnification of the eyepiece or camera tube. The specified magnification on the microscope objective housing is accurate as long as the objective is used with a compatible tube lens focal length.

Numerical Aperture (NA)
Numerical aperture, a measure of the acceptance angle of an objective, is a dimensionless quantity. It is commonly expressed as

NA = ni × sinθa

where θa is the maximum 1/2 acceptance angle of the objective, and ni is the index of refraction of the immersion medium. This medium is typically air, but may also be water, oil, or other substances.

Parfocal Length
Also referred to as the parfocal distance, this is the length from the top of the objective (at the base of the mounting thread) to the bottom of the cover glass (or top of the specimen in the case of objectives that are intended to be used without a cover glass). For instances in which the parfocal length needs to be increased, parfocal length extenders are available.

Working Distance
This is the distance between the front element of the objective and the specimen, depending on the design of the objective. The cover glass thickness specification engraved on the objective designates whether a cover glass should be used.

Field Number
The field number corresponds to the size of the field of view (in millimeters) multiplied by the objective's magnification.

FN = Field of View Diameter × Magnification

Correction Collar
Some objectives are equipped with a correction collar to compensate for differences in cover glass thickness or other media present between the objective and the plane of interest within the sample. The collar is used to adjust the relative position of an internal focusing element or group of elements. See the Correction Collar tab for more information.


Click to Enlarge

This graph shows the effect of a cover slip on image quality at 632.8 nm for a #1.5 cover glass (0.17 mm thickness) with a refractive index of 1.51.
2-Photon Objective Focusing with Spherical Correction
Click for Details
Thickness and Light Cone Dramatized for Readability

Correction Collar and Cover Glass Correction
A cover glass is often placed onto an aqueous specimen to create a flat top on a volume to image. While this makes focusing on a sample easier, the presence of the glass can introduce spherical aberrations in the final image. The graph to the right shows the magnitude of spherical aberration versus the thickness of the cover glass used, for 632.8 nm light. For the typical cover glass thickness of 0.17 mm, the spherical aberration caused by the cover glass does not exceed the diffraction-limited aberration for objectives with NA up to 0.40.  

Our TL10X-2P microscope objective is equipped with a correction collar, which compensates for cover glasses of different thickness by adjusting the relative position of internal optical elements, reducing the impact of spherical aberration to help achieve diffraction-limited performance. Its correction collar features two scales: one for the thickness of a cover glass with a refractive index of 1.51 and a second that corresponds to the optical thickness of the material between the objective and the focal plane (ignoring air). The latter is useful for cases where the region of interest is suspended in a solution. Values on both scales are given in units of millimeters. 

To find the optical thickness of all non-air elements between your objective and your sample, multiply the thickness of an element in millimeters by its refractive index, and find the sum of all such products. For example, suppose that the TL10X-2P objective is focusing through 7.30 mm of air, a 0.17 mm cover slip, and 1.00 mm of saline solution, as illustrated in the image to the lower right. To find the appropriate correction collar setting, multiply each thickness, t, by each material's refractive index, n, ignoring air:

Optical Thickness Collar Setting = ∑txnx 

t1n1 + t2n2 = 0.17 mm × 1.51 + 1.00 mm × 1.33 = 1.59 mm

Examples of Optical Thickness Calculationsa
Glass Thickness
(n = 1.51)
Water Thickness
(n = 1.33)
Optical Thickness
Collar Setting
0.00 mm 0.00 mm 0.00 mm
0.50 mm 0.00 mm 0.76 mm
0.17 mm 1.00 mm 1.59 mm
0.17 mm 3.00 mm 4.25 mm
  • Air volume is ignored for optical thickness calculations.

Our other super apochromatic objectives do not have a correction collar. While they are designed for use with a standard 0.17 mm thick cover glass, their NAs are low enough (0.2 or less) that any cover glass thickness between 0 and 0.5 mm can be used with little impact on image quality.

Widefield Viewing Optical Path
When viewing an image with a camera, the system magnification is the product of the objective and camera tube magnifications. When viewing an image with trinoculars, the system magnification is the product of the objective and eyepiece magnifications.
Magnification & FOV Calculator
Manufacturer Tube Lens
Focal Length
Leica f = 200 mm
Mitutoyo f = 200 mm
Nikon f = 200 mm
Olympus f = 180 mm
Thorlabs f = 200 mm
Zeiss f = 165 mm

The rows highlighted in green denote manufacturers that do not use f = 200 mm tube lenses.

Magnification and Sample Area Calculations

Magnification

The magnification of a system is the multiplicative product of the magnification of each optical element in the system. Optical elements that produce magnification include objectives, camera tubes, and trinocular eyepieces, as shown in the drawing to the right. It is important to note that the magnification quoted in these products' specifications is usually only valid when all optical elements are made by the same manufacturer. If this is not the case, then the magnification of the system can still be calculated, but an effective objective magnification should be calculated first, as described below.

To adapt the examples shown here to your own microscope, please use our Magnification and FOV Calculator, which is available for download by clicking on the red button above. Note the calculator is an Excel spreadsheet that uses macros. In order to use the calculator, macros must be enabled. To enable macros, click the "Enable Content" button in the yellow message bar upon opening the file.

Example 1: Camera Magnification
When imaging a sample with a camera, the image is magnified by the objective and the camera tube. If using a 20X Nikon objective and a 0.75X Nikon camera tube, then the image at the camera has 20X × 0.75X = 15X magnification.

Example 2: Trinocular Magnification
When imaging a sample through trinoculars, the image is magnified by the objective and the eyepieces in the trinoculars. If using a 20X Nikon objective and Nikon trinoculars with 10X eyepieces, then the image at the eyepieces has 20X × 10X = 200X magnification. Note that the image at the eyepieces does not pass through the camera tube, as shown by the drawing to the right.

Using an Objective with a Microscope from a Different Manufacturer

Magnification is not a fundamental value: it is a derived value, calculated by assuming a specific tube lens focal length. Each microscope manufacturer has adopted a different focal length for their tube lens, as shown by the table to the right. Hence, when combining optical elements from different manufacturers, it is necessary to calculate an effective magnification for the objective, which is then used to calculate the magnification of the system.

The effective magnification of an objective is given by Equation 1:

Equation 1 (Eq. 1)

Here, the Design Magnification is the magnification printed on the objective, fTube Lens in Microscope is the focal length of the tube lens in the microscope you are using, and fDesign Tube Lens of Objective is the tube lens focal length that the objective manufacturer used to calculate the Design Magnification. These focal lengths are given by the table to the right.

Note that Leica, Mitutoyo, Nikon, and Thorlabs use the same tube lens focal length; if combining elements from any of these manufacturers, no conversion is needed. Once the effective objective magnification is calculated, the magnification of the system can be calculated as before.

Example 3: Trinocular Magnification (Different Manufacturers)
When imaging a sample through trinoculars, the image is magnified by the objective and the eyepieces in the trinoculars. This example will use a 20X Olympus objective and Nikon trinoculars with 10X eyepieces.

Following Equation 1 and the table to the right, we calculate the effective magnification of an Olympus objective in a Nikon microscope:

Equation 2

The effective magnification of the Olympus objective is 22.2X and the trinoculars have 10X eyepieces, so the image at the eyepieces has 22.2X × 10X = 222X magnification.


Image Area on Camera

Sample Area When Imaged on a Camera

When imaging a sample with a camera, the dimensions of the sample area are determined by the dimensions of the camera sensor and the system magnification, as shown by Equation 2.

Equation 5 (Eq. 2)

The camera sensor dimensions can be obtained from the manufacturer, while the system magnification is the multiplicative product of the objective magnification and the camera tube magnification (see Example 1). If needed, the objective magnification can be adjusted as shown in Example 3.

As the magnification increases, the resolution improves, but the field of view also decreases. The dependence of the field of view on magnification is shown in the schematic to the right.

Example 4: Sample Area
The dimensions of the camera sensor in Thorlabs' 1501M-USB Scientific Camera are 8.98 mm × 6.71 mm. If this camera is used with the Nikon objective and trinoculars from Example 1, which have a system magnification of 15X, then the image area is:

Equation 6

Sample Area Examples

The images of a mouse kidney below were all acquired using the same objective and the same camera. However, the camera tubes used were different. Read from left to right, they demonstrate that decreasing the camera tube magnification enlarges the field of view at the expense of the size of the details in the image.

Image with 1X Camera Tube
Click to Enlarge

Acquired with 1X Camera Tube (Item # WFA4100)
Image with 1X Camera Tube
Click to Enlarge

Acquired with 0.75X Camera Tube (Item # WFA4101)
Image with 1X Camera Tube
Click to Enlarge

Acquired with 0.5X Camera Tube (Item # WFA4102)

Posted Comments:
Gabriel Martins  (posted 2019-07-17 06:54:01.707)
good morning, do you have information on how the 10x performs with cleared samples, ie, samples in medium with refractive index of ~1.52? Would it be possible to test the objective with our samples? Thanks in advance, Gaby
asundararaj  (posted 2019-07-23 10:08:15.0)
Thank you for contacting Thorlabs. Yes, the TL10X-2P objective can be used with cleared samples. You would need to set the spherical collar to the appropriate setting. You can find a procedure for calculating the correct setting in the Correction Collar tab of this page. I have contacted you directly about being able to offer a loan unit.
Denis Pristinski  (posted 2019-07-12 12:53:38.397)
Is Zemax black box model coming for TL10X-2P?
YLohia  (posted 2019-07-16 09:02:38.0)
Hello, thank you for contacting Thorlabs. The Zemax model for this can be found here : https://www.thorlabs.com/_sd.cfm?fileName=TTN142896-S02.zar&partNumber=TL10X-2P
a.scagliola2  (posted 2018-11-05 12:38:56.0)
Do you have a model of the objective with the principal planes?
nbayconich  (posted 2019-03-04 01:17:21.0)
Thank you for contacting Thorlabs. The principal plane will be located approximately 16mm in front of the objective lens or 16mm after the m25 x0.75 threading. I will reach out to you directly with more information.
a.andreski  (posted 2017-11-28 15:40:24.44)
Do these objectives have a flat field of view (are they plan)? If I use the TL2X-SAP with a TTL100A tubelens, what is the maximum object field of view that is possible without abberations? Thanks.
tfrisch  (posted 2017-12-15 04:35:36.0)
Hello, thank you for contacting Thorlabs. The Field of View will be flat, yes. TL2X-SAP will be diffraction limited over its full Field of View. I will reach out to you directly with details on the Strehl ratio.
ludoangot  (posted 2016-11-07 15:31:29.6)
Which tube lens do you recommend to match the extended transmission of these microscope lenses in the deep blue / UV (your recent TTL200 is for 400 to 700nm)?
tfrisch  (posted 2016-11-10 10:24:31.0)
Hello, thank you for contacting Thorlabs. I would typically recommend TTL200 which performs well over the range of TL2X-SAP and TL4X-SAP. You can see some performance graphs here: https://www.thorlabs.us/newgrouppage9.cfm?objectgroup_id=5834&tabname=Graphs

Super Apochromatic Objectives

  • High Numerical Aperture for Low-Light and Widefield Imaging
  • M25 x 0.75 Threading

These general-purpose objectives provide high-quality performance when used with a variety of imaging modalities and are useful for viewing large regions of a sample. The TL2X-SAP objective features an Ø11 mm field of view, large enough to capture the full length of an embryonic Zebrafish (Figure 1 below). The 4X magnification of the TLX4X-SAP can resolve finer details than its 2X counterpart, while the Ø5.5 mm field of view still provides a sense of scope and place within the sample (Figures 2 and 3 below). The long working distance of each objective provides ample room for micromanipulators.

The three images below should be viewed using ThorCam, ImageJ, or other scientific imaging software. They may not be displayed correctly in general-purpose image viewers.

Click to Enlarge
Click Here for Full-Size Image
Figure 1: Embryonic zebrafish embryo imaged using the TL2X-SAP objective, white light illumination, and a color camera. The image captures the full length of the zebrafish while still retaining fine details.
Click to Enlarge
Click Here for Full-Size Image
Figure 2: Dicot flower bud imaged using the TL4X-SAP objective and monochrome camera in a DIC setup.
Click to Enlarge
Click Here for Full-Size Image
Figure 3: Confocal fluorescence image of a mouse retina labeled with GFP, taken using the TL4X-SAP objective.
Key Specifications
Item # AR Coating
Wavelength Range
Mag. Numerical
Aperture
Working
Distance
Parfocal
Length
Cover Glass
Thickness
Threading Suitable Microscopy Techniques
Confocal Two-Photon Brightfield Darkfield Dodt DIC Fluorescence
TL2X-SAP 350 - 700 nm 2X 0.10 56.3 mm 95.0 mm 0 - 5.0 mm M25 x 0.75 -
TL4X-SAP 350 - 700 nm 4X 0.20 17.0 mm 60.0 mm 0 - 5.0 mm M25 x 0.75 -
Based on your currency / country selection, your order will ship from Newton, New Jersey  
+1 Qty Docs Part Number - Universal Price Available
TL2X-SAP Support Documentation
TL2X-SAP2X Super Apochromatic Microscope Objective, 0.1 NA, 56.3 mm WD
$1,176.67
Today
TL4X-SAP Support Documentation
TL4X-SAP4X Super Apochromatic Microscope Objective, 0.2 NA, 17.2 mm WD
$2,041.32
Today

Super Apochromatic Objective with Correction Collar

  • Dry Objective Ideal for Two-Photon Microscopy
  • Correction Collar Enables Use with Samples in a Variety of Media
  • M32 x 0.75 Threading

The TL10X-2P 10X Super Apochromatic Objective combines the diffraction limited axial color performance at visible wavelengths of our other objects with excellent transmission out to 1300 nm, making this objective ideal for multiphoton imaging applications (see the Graphs tab for details). It combines a high 0.50 NA for collecting two-photon fluorescence signals with a long 7.7 mm working distance, providing ample space for equipment to manipulate the sample. A correction collar allows adjustment for spherical aberrations introduced by imaging through aqueous solutions or thick cover glasses, without the need for water dipping or oil immersion (see the Correction Collar tab).

Note that for best performance, the front focal plane of your system's tube lens should be placed at the TL10X-2P's aperture stop. For most objectives, this stop coincides with the back element at the shoulder. This objective's aperture stop is located 42 mm from its shoulder. Without filling this stop, vingetting may occur. For example, Thorlabs' TTL200MP tube lens' focal plane is located at 228 mm from the shoulder of the tube lens. Therefore, the physical separation between the front element of the tube lens and the shoulder of the TL10X-2P objective should be 184 mm. This example is illustrated in the diagram below. 

Click to Enlarge
Click Here for Full-Size Image
Confocal fluorescence image of a mouse retina taken using the TL10X-2P objective. (Courtesy of Lynne Holtzclaw of the National Institutes of Health)
Alignment Diagram of TL10X-2P Objective and TTL200MP Tube Lens
Click to Enlarge
This diagram shows how to position the TL10X-2P relative to the TTL200MP tube lens so the entrance pupil is completely filled.
Key Specifications
Item # AR Coating
Wavelength Range
Mag. Numerical
Aperture
Working
Distance
Parfocal
Length
Cover Glass
Thickness
Threading Suitable Microscopy Techniques
Confocal Two-Photon Brightfield Darkfield Dodt DIC Fluorescence
TL10X-2P 400 - 1300 nm 10X 0.50 7.77 mm 95.0 mm 0 - 2.6 mm M32 x 0.75 -
Based on your currency / country selection, your order will ship from Newton, New Jersey  
+1 Qty Docs Part Number - Universal Price Available
TL10X-2P Support Documentation
TL10X-2PNEW!Customer Inspired! 10X Super Apochromatic Microscope Objective, 0.5 NA, 7.77 mm WD
$7,100.00
Today
Log In  |   My Account  |   Contact Us  |   Careers  |   Privacy Policy  |   Home  |   FAQ  |   Site Index
Regional Websites: West Coast US | Europe | Asia | China | Japan
Copyright 1999-2019 Thorlabs, Inc.
Sales: 1-973-300-3000
Technical Support: 1-973-300-3000


High Quality Thorlabs Logo 1000px:Save this Image