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Interconnects and Mating Sleeves for Fiber Optic Cannulae


BFY32FL1

Dual Core Patch Cable with FC/PC
Connectors on Split Ends and Ø2.5 mm
Stainless Steel Ferrule on Common End

ADAF1

Ceramic Split Mating Sleeve

Ø2.5 mm Dual-Core Stainless Steel Ferrule with Guide Pin

  • Connect Optogenetics Patch Cables and Cannulae
  • Quick-Release Interconnects Allow Simple,
    Low-Force Connections
  • Lightweight Ceramic Split Mating Sleeves

ADAF2

Interconnect for
Ø2.5 mm Ferrules

ADAF1

Ø2.5 mm Ceramic
Mating Sleeve

ADAL1

Ø1.25 mm Ceramic
Mating Sleeve

ADAL3 Quick-Release Interconnect Mating a Multimode Patch Cable and a Ø1.25 mm Cannula

ADAL3

Interconnect for
Ø1.25 mm Ferrules

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Thorlabs offers interconnects and mating sleeves for making connections between our line of optogenetics patch cables and fiber optic cannulae. These ferrule-mating components provide low-loss coupling and are compatible with both stainless steel and ceramic (zirconia) ferrules. The interconnect is designed to facilitate easy connections and disconnections from an implanted cannula, requiring <20% of the force needed to disconnect from mating sleeves. On the other hand, mating sleeves are preferred for very lightweight, low-profile connections between a patch cable and cannula.

Optogenetics Product Family for In Vivo Applications

Thorlabs offers a wide variety of products designed to support in vivo optogenetics applications. Please visit the OG Selection Guide tab above to see a full listing of available products for different applications.

Optogenetics System Schematic

This tab contains instructions for using an interconnect to mate an optogenetics patch cable with a Ø1.25 mm or Ø2.5 mm ferrule to a cannula of the same diameter. Please see the animation below for an illustration of the installation process detailed here.

Connecting the Patch Cable
Insert the patch cable ferrule into the interconnect on the side with the stainless steel setscrew. To secure the patch cable, use the included hex key [0.028" for the ADAL3 and 0.05" (1.3 mm) for the ADAF2] to tighten the setscrew onto the inserted ferrule.

  • Ensure that the patch cable ferrule tip is clean and free of dust; we recommend our canned compressed air.
  • For best results, the patch cable should be inserted past the setscrew and about halfway into the ceramic mating component within the interconnect.

Connecting the Cannula
To connect to a cannula, gently squeeze the interconnect from both sides where indicated by the engraving, then insert the cannula ferrule into the open end until physical contact with the patch cable ferrule is achieved. To lock the cannula in place, simply let go of the interconnect. 

  • Check that the ferrule tip of the cannula is clean and free of dust. 
  • If the cannula is already implanted or glued into position, adjust the cable-side ferrule, if necessary, to ensure physical contact between the ferrules.
  • When using the dual core cannula and patch cable, please note the orientation of the patch cable for alignment purposes.

Disconnecting the Cannula and Patch Cable
To disconnect the interconnect from a cannula, squeeze the interconnect and gently pull the interconnect until disconnected from the cannula. The patch cable can be released by loosening the setscrew with the included hex key. 


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Optogenetics Selection Guide

Thorlabs offers a wide range of optogenetics components; the compatibility of these products in select standard configurations is discussed in detail here. Please contact Technical Support for assistance with items outside the scope of this guide, including custom fiber components for optogenetics.

 

Single-Site Stimulation

One Light Source to One Cannula Implant

The most straightforward method for in vivo light stimulation of a specimen is to use a single fiber optic with a single LED light source. The single wavelength LED is powered by an LED driver, and then the illumination output is fiber-coupled into a patch cable, which connects to the implanted cannula. See the graphics and expandable compatibility tables below for the necessary patch cables and cannulae to create this setup. To choose the appropriate LED and driver, see below or the full web presentation.

Click on Each Component for More Information

LED DriverLED DriverFiber-Coupled LEDFiber-Coupled LEDSMA to Ferrule Patch Cable with Optional Rotary JointSMA to Ferrule Patch Cable with Optional Rotary JointADAF2 InterconnectADAF2 InterconnectFiber Optic CannulaFiber Optic Cannula



 

Bilateral Stimulation

The ability to accurately and simultaneously direct light to multiple locations within a specimen is desired for many types of optogenetics experiments. For example, bilateral stimulation techniques typically target neurons in two spatially separated regions in order to induce a desired behavior. In more complex experiments involving the simultaneous inhibition and stimulation of neurons, delivering light of two different monochromatic wavelengths within close proximity enables the user to perform these experiments without implanting multiple cannulae, which can increase stress on the specimen.

Bilateral stimulation can be achieved with several different configurations depending on the application requirements. The sections below illustrate examples of different configurations using Thorlabs' optogenetics products.


Option 1: One Light Source to Two Cannula Implants Using Rotary Joint Splitter

Thorlabs' RJ2 1x2 Rotary Joint Splitter is designed for optogenetics applications and is used to split light from a single input evenly between two outputs. The rotary joint interface allows connected patch cables to freely rotate, reducing the risk of fiber damage caused by a moving specimen. See the graphic and compatibility table below for the necessary cables and cannulae to create this setup. For LEDs and drivers, see below or the full web presentation.

LED DriverFiber-Coupled LEDHybrid Patch Cable1x2 Rotary Joint SplitterOG Patch CableOG Patch CableADAF2 InterconnectADAF2 InterconnectFiber Optic CannulaFiber Optic CannulaFiber Optic Cannula

Option 2: One or Two Light Sources to Two Cannula Implants

If the intent is for one LED source to connect to two cannulae for simultaneous light modulation, then a bifurcated fiber bundle can be used to split the light from the LED into each respective cannula. For dual wavelength stimulation (mixing two wavelengths in a single cannula) or a more controlled split ratio between cannula, one can use a multimode coupler to connect one or two LEDs to the cannulae. If one cable end is left unused, the spare coupler cable end may be terminated by a light trap. See the graphic and compatibility table below for the necessary cables and cannulae to create this setup. For LEDs and drivers, see below or the full web presentation.

Click on Each Component Below for More Information

LED DriverLED DriverFiber-Coupled LEDFiber-Coupled LEDPatch CabletextY-CableMating SleeveMating SleeveMating SleeveMating SleeveFiber Optic CannulaFiber Optic CannulaFiber Optic CannulaFiber Optic Cannula

LED Driver2nd LED DriverLED Driver textFiber-Coupled LED2nd Fiber-Coupled LEDFiber-Coupled LED textMultimode CouplerMultimode Coupler TextADAF2 InterconnectADAF2 Interconnect TextADAF2 InterconnectADAF2 Interconnect TextFiber Optic CannulaFiber Optic CannulaFiber Optic CannulaFiber Optic Cannula


 

Two Light Sources into One Dual-Core Cannula Implant

For bilateral stimulation applications where the two cannulas need to be placed in close proximity (within ~1 mm), Thorlabs offers dual-core patch cables and cannulae that are designed for this specific application. Each core is driven by a separate light source, enabling users to stimulate and/or supress nerve cells in the same region of the specimen. See the graphic and compatibility table below for the necessary cables and cannulae to create this setup. For LEDs and drivers, see below or the full web presentation.

LED Driver2nd LED DriverLED Driver TextFiber-Coupled LED2nd Fiber-Coupled LEDFiber-Coupled LEDsDual-Core Patch CableDual-Core Patch CableADAF2 InterconnectADAF2 InterconnectFiber Optic CannulaFiber Optic Cannula

Click on Each Component for More Information

Part Selection Table (Click Links for Item Description Popup)
Common Fiber Properties
Core Diameter 200 µm
Wavelength Range 400 - 2200 nm
NA 0.39
Fiber Type FT200EMT
Ferrule Stylea FC (Ø2.5 mm)
Dual-Core Patch Cable FC/PC Input BFY32FL1
SMA905 Input BFY32SL1
Compatible Mating Sleeve/Interconnect ADAF1
ADAF2
Dual-Core Fiber Optic Cannulaec Stainless Steel CFM32L10
CFM32L20
  • FC components have a Ø2.5 mm ferrule end.
  • Patch cables for dual light source to single implant applications are highlighted in green above. Choose a patch cable with an input that matches your light source.
  • Available cannulae are highlighted in orange of the table above. Cannule within the same column are interchangeable.

 

Popular Fiber-Coupled LEDs for Optogenetics
Item # M470F3 M590F2
Center Wavelength 470 nm 590 nm
Bandwidth (FWHM) 20 nm 15 nm
Typical Output Spectrum
(Click to Enlarge)
M470 Icon M470 Icon
Ø200 µm Core Fiber Output (Typ.)a
7.0 mW 0.68 mW
Ø400 µm Core Fiber Output (Typ.)b
21.8 mW 2.73 mW
CW Drive Current (Max) 1.0 A 1.0 A
LED Forward Voltage 3.1 V 2.8 V
Typical Lifetime >50,000 Hours >50,000 Hours
  • Tested using MM Fiber with Ø200 µm core, 0.22 NA (Item # FG200UCC).
  • Tested using MM Fiber with Ø400 µm core, 0.39 NA (Item # FT400EMT).

Illumination

Fiber-Coupled LEDs and Drivers

Our fiber-coupled LEDs are ideal light sources for optogenetics applications. They feature a variety of wavelength choices and a convenient interconnection to optogenetics patch cables. Thorlabs offers fiber-coupled LEDs with nominal wavelengths ranging from 280 nm to 1050 nm. See the table to the right for the LEDs with the most popular wavelengths for optogenetics. A table of compatible LED drivers can be viewed by clicking below.

Quick-Release Interconnects


Quick-Release Interconnect Usage
  • Quick-Release Interconnects for Mating Ø1.25 mm or Ø2.5 mm Ferrules
  • Easily Secure and Disconnect Ferrule with One-Handed Squeeze Operation
  • Low-Force Disconnections Minimize Stress on Specimen
  • Constructed from Virtually Nonmagnetic Materials
  • Minimal Light Leakage
  • Lightweight (≤1 g)

Click to Enlarge
Interconnects are easily operated using a single hand and are engraved to indicate where pressure should be applied.

Thorlabs' Quick-Release Interconnects are easy-to-use and reliable solutions for mating optogenetics patch cables and cannulae. These interconnects offer <1.0 dB (<21%) insertion loss and minimal light leakage at the interconnect interface, preventing stray light from distracting a specimen. The quick-release mechanism requires <20% of the force to connect or disconnect compared to mating sleeves, significantly reducing stress on a specimen when mating to a patch cable. As shown in the photo to the left, the interconnects can be operated using a single hand which ensures hassle-free mating to an implanted cannula.

As seen in the animation to the right, an interconnect holds a patch cable ferrule on one end using a cup-point setscrew that does not damage the ferrule surface, and holds a cannula on the other end using a quick-release squeeze mechanism. First, insert a patch cable ferrule into the interconnect and secure by tightening the setscrew using the included hex key. Then, squeeze the quick-release lever and insert the cannula until it makes physical contact with the ferrule. Finally, release the lever to lock the cannula into place. The interconnect is engraved to indicate where squeeze pressure should be applied. To release the cannula (or disconnect from an implanted cannula), squeeze the quick-release lever and pull the interconnect away from the cannula; only light force is needed to disconnect the interconnect. Please see the Interconnect Usage tab above for additional guidelines.

These interconnects are designed to be used with our optogenetics patch cables and cannulae and are not intended for use with patch cables terminated with standard connectors.

Item # Compatible
Ferrule Size
Insertion Loss Length Weight Disconnect Forcea
ADAL3 1.25 mm Outer Diameter <1.0 dB Typical Loss (Multimode)b 9.4 mm 0.4 g <0.89 N (<0.2 lbf) Typicalc
ADAF2 2.5 mm Outer Diameter <1.0 dB Typical Loss (Multimode)d 13.6 mm 1 g <4.4 N (<1.0 lbf) Typicale
  • Force needed to disconnect a ferrule when squeeze force is applied to quick-release.
  • Tested using Ø200 µm core, 0.22 NA multimode fiber and an LED source at 532 nm.
  • Tested using a LC ferrule.
  • Tested using Ø400 µm core, 0.39 NA multimode fiber and an LED source at 532 nm.
  • Tested using a FC ferrule.
Based on your currency / country selection, your order will ship from Newton, New Jersey  
+1 Qty Docs Part Number - Universal Price Available
ADAL3 Support Documentation
ADAL3Customer Inspired! Quick-Release Interconnect for Ø1.25 mm Ferrules
$31.37
Today
ADAF2 Support Documentation
ADAF2Customer Inspired! Quick-Release Interconnect for Ø2.5 mm Ferrules
$31.37
Today

Ceramic Mating Sleeves

Fiber Optic Cannula and Interconnect
Click for Details

 In the photo above, the ADAF1 is used to connect a cannula with a 2.5 mm ferrule to the ferrule end of a M81L01 patch cable.

Click to Enlarge
ADAF1 Mating Sleeve with Optogenetics Patch Cable and Cannula
  • Ceramic Mating Sleeves for Ø1.25 mm and Ø2.5 mm Ferrules
  • Connect Optogenetics Patch Cables and Cannulae
  • Very Lightweight (≤0.18 g)
  • Sold Individually or in Packs of 5

The ADAL1 and ADAF1 ceramic (zirconia) mating sleeves are low-profile, lightweight options for connecting our optogenetics patch cables with ferrule connectors to our implantable fiber optic cannulae (see photo to the right). Additionally, they are capable of connecting any terminated fiber with a Ø1.25 mm or Ø2.5 mm ferrule, such as LC/PC, FC/PC, ST®/PC, and SC/PC connectors. 

This mating sleeve is compatible with both stainless steel and ceramic patch cables and cannulae. Cannulae and patch cables with different ferrule materials can be mixed and matched without significant additional signal losses.

These mating sleeves are available individually or in packages of five at a significant savings over the individual price.

Item # Compatible Ferrule Size Insertion Loss Length Weight Disconnect Force Material
ADAL1 1.25 mm Outer Diameter <0.5 dB Typical (Single Mode)a
<1.0 dB Typical (Multimode)b
6.8 mm 0.05 g 11.4 N (2.6 lbf) Maxc
7.2 N (1.6 lbf) Typicalc
Ceramic
(Zirconia)
ADAF1 2.5 mm Outer Diameter <0.2 dB Typical (Single Mode)d
<1.0 dB Typical (Multimode Fiber)
e
11.4 mm 0.18 g 53 N (12 lbf) Maxf
25 N (5.6 lbf) Typicalf
  • Tested using SM600 fiber at 635 nm with LC/PC connectors.
  • Tested using Ø200 µm core, 0.39 NA multimode fiber with LC/PC connectors and an LED source at 617 nm.
  • Tested using an LC ferrule.
  • Tested using SMF28 fiber at 1550 nm with FC/PC connectors.
  • Tested using Ø200 µm core, 0.39 NA multimode fiber with FC/PC connectors and an LED source at 617 nm.
  • Tested using an FC ferrule.
Based on your currency / country selection, your order will ship from Newton, New Jersey  
+1 Qty Docs Part Number - Universal Price Available
ADAL1 Support Documentation
ADAL1Ceramic Split Mating Sleeve for Ø1.25 mm (LC/PC) Ferrules
$4.68
Today
ADAL1-5 Support Documentation
ADAL1-5Ceramic Split Mating Sleeves for Ø1.25 mm (LC/PC) Ferrules, 5 Pack
$18.73
Today
ADAF1 Support Documentation
ADAF1Ceramic Split Mating Sleeve for Ø2.5 mm (FC/PC, ST®/PC, or SC/PC) Ferrules
$5.20
Today
ADAF1-5 Support Documentation
ADAF1-5Ceramic Split Mating Sleeves for Ø2.5 mm (FC/PC, ST®/PC, or SC/PC) Ferrules, 5 Pack
$20.81
Today
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