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Interconnects and Mating Sleeves for Fiber Optic Cannulae
Dual Core Patch Cable with FC/PC
Ceramic Split Mating Sleeve
Ø2.5 mm Dual-Core Stainless Steel Ferrule with Guide Pin
Ø2.5 mm Ceramic
Ø1.25 mm Ceramic
ADAL3 Quick-Release Interconnect Mating a Multimode Patch Cable and a Ø1.25 mm Cannula
Thorlabs offers interconnects and mating sleeves for making connections between our line of optogenetics patch cables and fiber optic cannulae. These ferrule-mating components provide low-loss coupling and are compatible with both stainless steel and ceramic (zirconia) ferrules. The interconnect is designed to facilitate easy connections and disconnections from an implanted cannula, requiring <20% of the force needed to disconnect from mating sleeves. On the other hand, mating sleeves are preferred for very lightweight, low-profile connections between a patch cable and cannula.
Optogenetics Product Family for In Vivo Applications
Thorlabs offers a wide variety of products designed to support in vivo optogenetics applications. Please visit the OG Selection Guide tab above to see a full listing of available products for different applications.
This tab contains instructions for using an interconnect to mate an optogenetics patch cable with a Ø1.25 mm or Ø2.5 mm ferrule to a cannula of the same diameter. Please see the animation below for an illustration of the installation process detailed here.
Connecting the Patch Cable
Connecting the Cannula
Disconnecting the Cannula and Patch Cable
Optogenetics Selection Guide
Thorlabs offers a wide range of optogenetics components; the compatibility of these products in select standard configurations is discussed in detail here. Please contact Technical Support for assistance with items outside the scope of this guide, including custom fiber components for optogenetics.
The most straightforward method for in vivo light stimulation of a specimen is to use a single fiber optic with a single LED light source. The single wavelength LED is powered by an LED driver, and then the illumination output is fiber-coupled into a patch cable, which connects to the implanted cannula. See the graphics and expandable compatibility tables below for the necessary patch cables and cannulae to create this setup. To choose the appropriate LED and driver, see below or the full web presentation.
Click on Each Component for More Information
Click to See Ø1.25 mm (LC) Ferrule Compatible Patch Cables, Cannulae, and Interconnects
Click to See Ø2.5 mm (FC) Ferrule Compatible Patch Cables, Cannulae, and Interconnects
The ability to accurately and simultaneously direct light to multiple locations within a specimen is desired for many types of optogenetics experiments. For example, bilateral stimulation techniques typically target neurons in two spatially separated regions in order to induce a desired behavior. In more complex experiments involving the simultaneous inhibition and stimulation of neurons, delivering light of two different monochromatic wavelengths within close proximity enables the user to perform these experiments without implanting multiple cannulae, which can increase stress on the specimen.
Bilateral stimulation can be achieved with several different configurations depending on the application requirements. The sections below illustrate examples of different configurations using Thorlabs' optogenetics products.
Thorlabs' RJ2 1x2 Rotary Joint Splitter is designed for optogenetics applications and is used to split light from a single input evenly between two outputs. The rotary joint interface allows connected patch cables to freely rotate, reducing the risk of fiber damage caused by a moving specimen. See the graphic and compatibility table below for the necessary cables and cannulae to create this setup. For LEDs and drivers, see below or the full web presentation.
Click to See Ø1.25 mm (LC) Ferrule Components Recommended for Use with RJ2 Rotary Joint Splitter
Click to See Ø2.5 mm (FC) Ferrule Components Recommended for Use with RJ2 Rotary Joint Splitter
If the intent is for one LED source to connect to two cannulae for simultaneous light modulation, then a bifurcated fiber bundle can be used to split the light from the LED into each respective cannula. For dual wavelength stimulation (mixing two wavelengths in a single cannula) or a more controlled split ratio between cannula, one can use a multimode coupler to connect one or two LEDs to the cannulae. If one cable end is left unused, the spare coupler cable end may be terminated by a light trap. See the graphic and compatibility table below for the necessary cables and cannulae to create this setup. For LEDs and drivers, see below or the full web presentation.
Click on Each Component Below for More Information
For bilateral stimulation applications where the two cannulas need to be placed in close proximity (within ~1 mm), Thorlabs offers dual-core patch cables and cannulae that are designed for this specific application. Each core is driven by a separate light source, enabling users to stimulate and/or supress nerve cells in the same region of the specimen. See the graphic and compatibility table below for the necessary cables and cannulae to create this setup. For LEDs and drivers, see below or the full web presentation.
Click on Each Component for More Information
Click to Enlarge
Our fiber-coupled LEDs are ideal light sources for optogenetics applications. They feature a variety of wavelength choices and a convenient interconnection to optogenetics patch cables. Thorlabs offers fiber-coupled LEDs with nominal wavelengths ranging from 280 nm to 1050 nm. See the table to the right for the LEDs with the most popular wavelengths for optogenetics. A table of compatible LED drivers can be viewed by clicking below.
Quick-Release Interconnect Usage
Click to Enlarge
Interconnects are easily operated using a single hand and are engraved to indicate where pressure should be applied.
Thorlabs' Quick-Release Interconnects are easy-to-use and reliable solutions for mating optogenetics patch cables and cannulae. These interconnects offer <1.0 dB (<21%) insertion loss and minimal light leakage at the interconnect interface, preventing stray light from distracting a specimen. The quick-release mechanism requires <20% of the force to connect or disconnect compared to mating sleeves, significantly reducing stress on a specimen when mating to a patch cable. As shown in the photo to the left, the interconnects can be operated using a single hand which ensures hassle-free mating to an implanted cannula.
As seen in the animation to the right, an interconnect holds a patch cable ferrule on one end using a cup-point setscrew that does not damage the ferrule surface, and holds a cannula on the other end using a quick-release squeeze mechanism. First, insert a patch cable ferrule into the interconnect and secure by tightening the setscrew using the included hex key. Then, squeeze the quick-release lever and insert the cannula until it makes physical contact with the ferrule. Finally, release the lever to lock the cannula into place. The interconnect is engraved to indicate where squeeze pressure should be applied. To release the cannula (or disconnect from an implanted cannula), squeeze the quick-release lever and pull the interconnect away from the cannula; only light force is needed to disconnect the interconnect. Please see the Interconnect Usage tab above for additional guidelines.
These interconnects are designed to be used with our optogenetics patch cables and cannulae and are not intended for use with patch cables terminated with standard connectors.
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In the photo above, the ADAF1 is used to connect a cannula with a 2.5 mm ferrule to the ferrule end of a M81L01 patch cable.
Click to Enlarge
ADAF1 Mating Sleeve with Optogenetics Patch Cable and Cannula
The ADAL1 and ADAF1 ceramic (zirconia) mating sleeves are low-profile, lightweight options for connecting our optogenetics patch cables with ferrule connectors to our implantable fiber optic cannulae (see photo to the right). Additionally, they are capable of connecting any terminated fiber with a Ø1.25 mm or Ø2.5 mm ferrule, such as LC/PC, FC/PC, ST®/PC, and SC/PC connectors.
This mating sleeve is compatible with both stainless steel and ceramic patch cables and cannulae. Cannulae and patch cables with different ferrule materials can be mixed and matched without significant additional signal losses.
These mating sleeves are available individually or in packages of five at a significant savings over the individual price.