1.6 MP CMOS Compact Scientific Cameras

Insights into Mounting Lenses to Thorlabs' Scientific Cameras

Scroll down to read about compatibility between lenses and cameras of different mount types, with a focus on Thorlabs' scientific cameras.

• Can C-mount and CS-mount cameras and lenses be used with each other?
• Do Thorlabs' scientific cameras need an adapter?
• Why can the FFD be smaller than the distance separating the camera's flange and sensor?

Click here for more insights into lab practices and equipment.

Can C-mount and CS-mount cameras and lenses be used with each other?

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Figure 1: C-mount lenses and cameras have the same flange focal distance (FFD), 17.526 mm. This ensures light through the lens focuses on the camera's sensor. Both components have 1.000"-32 threads, sometimes referred to as "C-mount threads".

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Figure 2: CS-mount lenses and cameras have the same flange focal distance (FFD), 12.526 mm. This ensures light through the lens focuses on the camera's sensor. Their 1.000"-32 threads are identical to threads on C-mount components, sometimes referred to as "C-mount threads."

The C-mount and CS-mount camera system standards both include 1.000"-32 threads, but the two mount types have different flange focal distances (FFD, also known as flange focal depth, flange focal length, register, flange back distance, and flange-to-film distance). The FFD is 17.526 mm for the C-mount and 12.526 mm for the CS-mount (Figures 1 and 2, respectively).

Since their flange focal distances are different, the C-mount and CS-mount components are not directly interchangeable. However, with an adapter, it is possible to use a C-mount lens with a CS-mount camera.

Mixing and Matching
C-mount and CS-mount components have identical threads, but lenses and cameras of different mount types should not be directly attached to one another. If this is done, the lens' focal plane will not coincide with the camera's sensor plane due to the difference in FFD, and the image will be blurry.

With an adapter, a C-mount lens can be used with a CS-mount camera (Figures 3 and 4). The adapter increases the separation between the lens and the camera's sensor by 5.0 mm, to ensure the lens' focal plane aligns with the camera's sensor plane.

In contrast, the shorter FFD of CS-mount lenses makes them incompatible for use with C-mount cameras (Figure 5). The lens and camera housings prevent the lens from mounting close enough to the camera sensor to provide an in-focus image, and no adapter can bring the lens closer.

It is critical to check the lens and camera parameters to determine whether the components are compatible, an adapter is required, or the components cannot be made compatible.

1.000"-32 Threads
Imperial threads are properly described by their diameter and the number of threads per inch (TPI). In the case of both these mounts, the thread diameter is 1.000" and the TPI is 32. Due to the prevalence of C-mount devices, the 1.000"-32 thread is sometimes referred to as a "C-mount thread." Using this term can cause confusion, since CS-mount devices have the same threads.

Measuring Flange Focal Distance
Measurements of flange focal distance are given for both lenses and cameras. In the case of lenses, the FFD is measured from the lens' flange surface (Figures 1 and 2) to its focal plane. The flange surface follows the lens' planar back face and intersects the base of the external 1.000"-32 threads. In cameras, the FFD is measured from the camera's front face to the sensor plane. When the lens is mounted on the camera without an adapter, the flange surfaces on the camera front face and lens back face are brought into contact.

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Figure 5: A CS-mount lens is not directly compatible with a C-mount camera, since the light focuses before the camera's sensor. Adapters are not useful, since the solution would require shrinking the flange focal distance of the camera (blue arrow).

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Figure 4: An adapter with the proper thickness moves the C-mount lens away from the CS-mount camera's sensor by an optimal amount, which is indicated by the length of the purple arrow. This allows the lens to focus light on the camera's sensor, despite the difference in FFD.

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Figure 3: A C-mount lens and a CS-mount camera are not directly compatible, since their flange focal distances, indicated by the blue and yellow arrows, respectively, are different. This arrangement will result in blurry images, since the light will not focus on the camera's sensor.

Date of Last Edit: July 21, 2020

Do Thorlabs' scientific cameras need an adapter?

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Figure 6: An adapter can be used to optimally position a C-mount lens on a camera whose flange focal distance is less than 17.526 mm. This sketch is based on a Zelux camera and its SM1A10Z adapter.

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Figure 7: An adapter can be used to optimally position a CS-mount lens on a camera whose flange focal distance is less than 12.526 mm. This sketch is based on a Zelux camera and its SM1A10 adapter.

All Kiralux™ and Quantalux^® scientific cameras are factory set to accept C-mount lenses. When the attached C-mount adapters are removed from the passively cooled cameras, the SM1 (1.035"-40) internal threads in their flanges can be used. The Zelux scientific cameras also have SM1 internal threads in their mounting flanges, as well as the option to use a C-mount or CS-mount adapter.

The SM1 threads integrated into the camera housings are intended to facilitate the use of lens assemblies created from Thorlabs components. Adapters can also be used to convert from the camera's C-mount configurations. When designing an application-specific lens assembly or considering the use of an adapter not specifically designed for the camera, it is important to ensure that the flange focal distances (FFD) of the camera and lens match, as well as that the camera's sensor size accommodates the desired field of view (FOV).

Made for Each Other: Cameras and Their Adapters
Fixed adapters are available to configure the Zelux cameras to meet C-mount and CS-mount standards (Figures 6 and 7). These adapters, as well as the adjustable C-mount adapters attached to the passively cooled Kiralux and Quantalux cameras, were designed specifically for use with their respective cameras.

While any adapter converting from SM1 to 1.000"-32 threads makes it possible to attach a C-mount or CS-mount lens to one of these cameras, not every thread adapter aligns the lens' focal plane with a specific camera's sensor plane. In some cases, no adapter can align these planes. For example, of these scientific cameras, only the Zelux can be configured for CS-mount lenses.

The position of the lens' focal plane is determined by a combination of the lens' FFD, which is measured in air, and any refractive elements between the lens and the camera's sensor. When light focused by the lens passes through a refractive element, instead of just travelling through air, the physical focal plane is shifted to longer distances by an amount that can be calculated. The adapter must add enough separation to compensate for both the camera's FFD, when it is too short, and the focal shift caused by any windows or filters inserted between the lens and sensor.

Flexiblity and Quick Fixes: Adjustable C-Mount Adapter
Passively cooled Kiralux and Quantalux cameras consist of a camera with SM1 internal threads, a window or filter covering the sensor and secured by a retaining ring, and an adjustable C-mount adapter.

A benefit of the adjustable C-mount adapter is that it can tune the spacing between the lens and camera over a 1.8 mm range, when the window / filter and retaining ring are in place. Changing the spacing can compensate for different effects that otherwise misalign the camera's sensor plane and the lens' focal plane. These effects include material expansion and contraction due to temperature changes, positioning errors from tolerance stacking, and focal shifts caused by a substitute window or filter with a different thickness or refractive index.

Adjusting the camera's adapter may be necessary to obtain sharp images of objects at infinity. When an object is at infinity, the incoming rays are parallel, and location of the focus defines the FFD of the lens. Since the actual FFDs of lenses and cameras may not match their intended FFDs, the focal plane for objects at infinity may be shifted from the sensor plane, resulting in a blurry image.

If it is impossible to get a sharp image of objects at infinity, despite tuning the lens focus, try adjusting the camera's adapter. This can compensate for shifts due to tolerance and environmental effects and bring the image into focus.

Date of Last Edit: Aug. 2, 2020

Why can the FFD be smaller than the distance separating the camera's flange and sensor?

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Figure 9: Refraction causes the ray's angle with the optical axis to be shallower in the medium than in air (θ_m vs. θ_o ), due to the differences in refractive indices (n_m vs. n_o ). After travelling a distance d in the medium, the ray is only h_m closer to the axis. Due to this, the ray intersects the axis Δf beyond the f point.;

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Figure 8: A ray travelling through air intersects the optical axis at point f. The ray is h_o closer to the axis after it travels across distance d. The refractive index of the air is n_o .

Equations for Calculating the Focal Shift (Δf )
Angle of Ray in Air, from Lens f-Number ( f / N )
Change in Distance to Axis, Travelling through Air (Figure 8)
Angle of Ray to Axis, in the Medium (Figure 9)
Change in Distance to Axis, Travelling through Optic (Figure 9)
Focal Shift Caused by Refraction through Medium (Figure 9)	Exact Calculation
	Paraxial Approximation

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Figure 11: Tolerance and / or temperature effects may result in the lens and camera having different FFDs. If the FFD of the lens is shorter, images of objects at infinity will be excluded from the focal range. Since the system cannot focus on them, they will be blurry.

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Figure 10: When their flange focal distances (FFD) are the same, the camera's sensor plane and the lens' focal plane are perfectly aligned. Images of objects at infinity coincide with one limit of the system's focal range.

Flange focal distance (FFD) values for cameras and lenses assume only air fills the space between the lens and the camera's sensor plane. If windows and / or filters are inserted between the lens and camera sensor, it may be necessary to increase the distance separating the camera's flange and sensor planes to a value beyond the specified FFD. A span equal to the FFD may be too short, because refraction through windows and filters bends the light's path and shifts the focal plane farther away.

If making changes to the optics between the lens and camera sensor, the resulting focal plane shift should be calculated to determine whether the separation between lens and camera should be adjusted to maintain good alignment. Note that good alignment is necessary for, but cannot guarantee, an in-focus image, since new optics may introduce aberrations and other effects resulting in unacceptable image quality.

A Case of the Bends: Focal Shift Due to Refraction
While travelling through a solid medium, a ray's path is straight (Figure 8). Its angle (θ_o ) with the optical axis is constant as it converges to the focal point (f ). Values of FFD are determined assuming this medium is air.

When an optic with plane-parallel sides and a higher refractive index (n_m ) is placed in the ray's path, refraction causes the ray to bend and take a shallower angle (θ_m ) through the optic. This angle can be determined from Snell's law, as described in the table and illustrated in Figure 9.

While travelling through the optic, the ray approaches the optical axis at a slower rate than a ray travelling the same distance in air. After exiting the optic, the ray's angle with the axis is again θ_o , the same as a ray that did not pass through the optic. However, the ray exits the optic farther away from the axis than if it had never passed through it. Since the ray refracted by the optic is farther away, it crosses the axis at a point shifted Δf beyond the other ray's crossing. Increasing the optic's thickness widens the separation between the two rays, which increases Δf.

To Infinity and Beyond
It is important to many applications that the camera system be capable of capturing high-quality images of objects at infinity. Rays from these objects are parallel and focused to a point closer to the lens than rays from closer objects (Figure 9). The FFDs of cameras and lenses are defined so the focal point of rays from infinitely distant objects will align with the camera's sensor plane. When a lens has an adjustable focal range, objects at infinity are in focus at one end of the range and closer objects are in focus at the other.

Different effects, including temperature changes and tolerance stacking, can result in the lens and / or camera not exactly meeting the FFD specification. When the lens' actual FFD is shorter than the camera's, the camera system can no longer obtain sharp images of objects at infinity (Figure 11). This offset can also result if an optic is removed from between the lens and camera sensor.

An approach some lenses use to compensate for this is to allow the user to vary the lens focus to points "beyond" infinity. This does not refer to a physical distance, it just allows the lens to push its focal plane farther away. Thorlabs' Kiralux™ and Quantalux^® cameras include adjustable C-mount adapters to allow the spacing to be tuned as needed.

If the lens' FFD is larger than the camera's, images of objects at infinity fall within the system's focal range, but some closer objects that should be within this range will be excluded. This situation can be caused by inserting optics between the lens and camera sensor. If objects at infinity can still be imaged, this can often be acceptable.

Not Just Theory: Camera Design Example
The C-mount, hermetically sealed, and TE-cooled Quantalux camera has a fixed 18.1 mm spacing between its flange surface and sensor plane. However, the FFD (f ) for C-mount camera systems is 17.526 mm. The camera's need for greater spacing becomes apparent when the focal shift due to the window soldered into the hermetic cover and the glass covering the sensor are taken into account. The results recorded in the table beneath Figure 9 show that both exact and paraxial equations return a required total spacing of 18.1 mm.

Date of Last Edit: July 31, 2020

Thorlabs offers four families of scientific cameras: Zelux™, Kiralux^®, Quantalux^®, and Scientific CCD. Zelux cameras are designed for general-purpose imaging and provide high imaging performance while maintaining a small footprint. Kiralux cameras have CMOS sensors in compact, passively cooled housings and are available with monochrome, color, NIR-enhanced, or polarization-sensitive sensors. The polarization-sensitive Kiralux camera incorporates an integrated micropolarizer array that, when used with our ThorCam™ software package, captures images that illustrate degree of linear polarization, azimuth, and intensity at the pixel level. Our Quantalux monochrome sCMOS cameras feature high dynamic range combined with extremely low read noise for low-light applications. They are available in either a compact passively cooled housing or a hermetically sealed TE-cooled housing. We also offer scientific CCD cameras with a variety of features, including versions optimized for operation at UV, visible, or NIR wavelengths; fast-frame-rate cameras; TE-cooled or non-cooled housings; and versions with the sensor face plate removed. The tables below provide a summary of our camera offerings.

Compact Scientific Cameras
Camera Type	Zelux™ CMOS	Kiralux® CMOS					Quantalux® sCMOS
	1.6 MP	1.3 MP	2.3 MP	5 MP	8.9 MP	12.3 MP	2.1 MP
Item #	Monochrome: CS165MUa Color: CS165CUa	Mono.: CS135MU Color: CS135CU NIR-Enhanced Mono.: CS135MUN	Mono.: CS235MU Color: CS235CU	Mono.: CS505MU Color: CS505CU Polarization: CS505MUP	Mono.: CS895MU Color: CS895CU	Mono.: CS126MU Color: CS126CU	Monochrome, Passive Cooling: CS2100M-USB Active Cooling: CC215MU
Product Photos (Click to Enlarge)
Electronic Shutter	Global Shutter	Global Shutter					Rolling Shutterb
Sensor Type	CMOS	CMOS					sCMOS
Number of Pixels (H x V)	1440 x 1080	1280 x 1024	1920 x 1200	2448 x 2048	4096 x 2160	4096 x 3000	1920 x 1080
Pixel Size	3.45 µm x 3.45 µm	4.8 µm x 4.8 µm	5.86 µm x 5.86 µm	3.45 µm x 3.45 µm			5.04 µm x 5.04 µm
Optical Format	1/2.9" (6.2 mm Diag.)	1/2" (7.76 mm Diag.)	1/1.2" (13.4 mm Diag.)	2/3" (11 mm Diag.)	1" (16 mm Diag.)	1.1" (17.5 mm Diag.)	2/3" (11 mm Diag.)
Peak Quantum Efficiency (Click for Plot)	Monochrome: 69% at 575 nm Color: Click for Plot	Monochrome: 59% at 550 nm Color: Click for Plot NIR: 60% at 600 nm	Monochrome: 78% at 500 nm Color: Click for Plot	Monochrome & Polarization: 72% (525 to 580 nm) Color: Click for Plot	Monochrome: 72% (525 to 580 nm) Color: Click for Plot	Monochrome: 72% (525 to 580 nm) Color: Click for Plot	Monochrome: 61% (at 600 nm)
Max Frame Rate (Full Sensor)	34.8 fps	92.3 fps	39.7 fps	35 fps	20.8 fps	14.6 fps	50 fps
Read Noise	<4.0 e- RMS	<7.0 e- RMS	<7.0 e- RMS	<2.5 e- RMS			<1 e- Median RMS; <1.5 e- RMS
Digital Output	10 Bit (Max)	10 Bit (Max)	12 Bit (Max)				16 Bit (Max)
PC Interface	USB 3.0
Available Fanless Cooling	Passive Thermal Management						0 °C at 20 °C Ambient
Housing Size (Click for Details)	0.59" x 1.72" x 1.86" (15.0 x 43.7 x 47.2 mm3)	2.77" x 2.38" x 1.88" (70.4 mm x 60.3 mm x 47.6 mm)					Passively Cooled sCMOS Camera TE-Cooled sCMOS Camera
Typical Applications	General Purpose Imaging, Brightfield Microscopy, Machine Vision & Robotics, UAV, Drone, & Handheld Imaging, Inspection, Monitoring	VIS/NIR Imaging, Electrophysiology/Brain Slice Imaging, Materials Inspection, Multispectral Imaging, Ophthalmology/Retinal Imaging, Vascular Imaging, Laser Speckle Imaging, Semiconductor Inspection, Fluorescence Microscopy, Brightfield Microscopy	Fluorescence Microscopy, Immunohistochemistry, Machine Vision, Inspection, General Purpose Imaging	Mono. & Color: Fluorescence Microscopy, Immunohistochemistry, Machine Vision & Inspection, Polarization: Machine Vision & Inspection, Transparent Material Detection, Surface Reflection Reduction	Fluorescence Microscopy, Immunohistochemistry, Large FOV Slide Imaging, Machine Vision, Inspection		Fluorescence Microscopy, VIS/NIR Imaging, Quantum Dots, Autofluorescence, Materials Inspection, Multispectral Imaging

• These item numbers are representative of the Zelux family. These cameras are available with or without external hardware triggers.
• Rolling Shutter with Equal Exposure Pulse (EEP) Mode for Synchronizing the Camera and Light Sources for Even Illumination

Scientific CCD Cameras
Camera Type	Fast Frame Rate VGA CCD		1.4 MP CCD	4 MP CCD	8 MP CCD
Item # Prefix	Monochrome: 340M	UV-Enhanced Monochrome: 340UV	Monochrome: 1501M Color: 1501C	Monochrome: 4070M Color: 4070C	Monochrome: 8051M Color: 8051C	Monochrome, No Sensor Face Plate: S805MU
Product Photo (Click to Enlarge)
Electronic Shutter	Global Shutter
Sensor Type	CCD
Number of Pixels (H x V)	640 x 480		1392 x 1040	2048 x 2048	3296 x 2472
Pixel Size	7.4 µm x 7.4 µm		6.45 µm x 6.45 µm	7.4 µm x 7.4 µm	5.5 µm x 5.5 µm
Optical Format	1/3" (5.92 mm Diagonal)		2/3" (11 mm Diagonal)	4/3" (21.4 mm Diagonal)	4/3" (22 mm Diagonal)
Peak QE (Click for Plot)	55% at 500 nm	10% at 485 nm	Monochrome: 60% at 500 nm Color: Click for Plot	Monochrome: 52% at 500 nm Color: Click for Plot	Monochrome: 51% at 460 nm Color: Click for Plot	51% at 460 nm
Max Frame Rate (Full Sensor)	200.7 fps (at 40 MHz Dual-Tap Readout)		23 fps (at 40 MHz Single-Tap Readout)	25.8 fps (at 40 MHz Quad-Tap Readout)a	17.1 fps (at 40 MHz Quad-Tap Readout)b	17.1 fps (at 40 MHz Quad-Tap Readout)
Read Noise	<15 e- at 20 MHz		<7 e- at 20 MHz (Standard Models) <6 e- at 20 MHz (-TE Models)	<12 e- at 20 MHz	<10 e- at 20 MHz
Digital Output (Max)	14 Bitc		14 Bit	14 Bitc		14 Bit
Available Fanless Cooling	Passive Thermal Management		-20 °C at 20 °C Ambient Temperature		-10 °C at 20 °C Ambient	Passive Thermal Management
Available PC Interfaces	USB 3.0 or Gigabit Ethernet					USB 3.0
Housing Dimensions (Click for Details)	Non-Cooled Scientific CCD Camera		Cooled Scientific CCD Camera Non-Cooled Scientific CCD Camera			No Face Plate Scientific CCD Camera
Typical Applications	Ca++ Ion Imaging, Particle Tracking, Flow Cytometry, SEM/EBSD, UV Inspection		Fluorescence Microscopy, VIS/NIR Imaging, Quantum Dots, Multispectral Imaging, Immunohistochemistry (IHC), Retinal Imaging	Fluorescence Microscopy, Transmitted Light Micrsoscopy, Whole-Slide Microscopy, Electron Microscopy (TEM/SEM), Inspection, Material Sciences	Fluorescence Microscopy, Whole-Slide Microscopy, Large FOV Slide Imaging, Histopathology, Inspection, Multispectral Imaging, Immunohistochemistry (IHC)	Beam Profiling & Characterization, Interferometry, VCSEL Inspection, Quantitative Phase-Contrast Microscopy, Ptychography, Digital Holographic Microscopy

• Limited to 13 fps at 40 MHz dual-tap readout for Gigabit Ethernet cameras; quad-tap readout is unavailable for Gigabit Ethernet cameras.
• Limited to 8.5 fps at 40 MHz dual-tap readout for Gigabit Ethernet cameras; quad-tap readout is unavailable for Gigabit Ethernet cameras.
• Gigabit Ethernet cameras operating in dual-tap readout mode are limited to 12-bit digital output.