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Two-Camera Mount for Microscopes


  • Mount Two Cameras for Two-Channel Imaging
  • Compatible with Thorlabs' Cerna Series and Other Microscopes
  • Designed for Thorlabs' Scientific Cameras

2SCM1-DC

2SCM1-DC Two Camera Mount on an Upright Microscope with Two 1500M-GE Scientific Cameras

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Suggested Compatible Optics
Fluorescence Imaging Filters
(Dichroic Mirrors, Emission Filters, Excitation Filters)
Longpass and Shortpass Dichroic Mirrors
Hot and Cold Mirrors
Plate Beamsplitters
Nir Dodt and Fluorescence Imaging
Click to Enlarge
The image above shows a live, simultaneous overlay of fluorescence and near-infrared Dodt contrast images of a 50 µm brain section from a CX3CR1-GFP mouse. More details can be found on the Applications tab. Sample courtesy of Dr. Andrew Chojnacki, University of Calgary.

Features

  • Microscope Adapter Allows Two Scientific Cameras to Image a Single Optical Input
  • Ideal for Calcium Ratio Imaging and Electrophysiology
  • Accepts 25 mm x 36 mm Dichroic Filters or Beamsplitters
  • Accepts Standard Ø25 mm or Ø1" Filters on Outputs
  • Fine Pitch Rotation and XY Adjustment for Image Registration
  • 6 mm Coarse Focus Adjustment for Parfocalization
  • Adapts to Most Standard Microscopes Using Thorlabs' Standard SM1 Interface (Microscope Camera Port Adapters Sold Separately)
  • Use with ThorCamTM Image Overlay Plug-in

Thorlabs' Two-Camera Mount, designed to be used with Thorlabs' scientific cameras, can attach two cameras to a standard upright microscope, allowing simultaneous imaging of a single optical output. Typical applications include multispectral imaging using a dichroic beamsplitter; for more details please see the Applications tab. A rotation mount allows for 360° of rotational adjustment (±8° fine adjustment) for the reflected camera while a translation mount gives 4 mm linear XY adjustment of the transmitted camera. Both camera ports have up to 6 mm of coarse focus adjustment by manually translating the cameras, allowing for parfocalization of both images.

The mount includes our Fluorescence Filter Cube, which is designed to hold a fluorescence filter set (dichroic mirror, excitation filter, and emission filter) as well as 25 mm x 36 mm plate beamsplitters or other optics (up to 1.1 mm thick). The filter cube consists of a base and top lid with an insert to hold filter set components and has a kinematic design for easy swapping between mounted filter sets without requiring realignment. The includes one top piece for mounting a filter set; DFM1T1 filter cube tops for mounting additional filter sets are sold separately below.

Microscope Compatibility
The input port of the 2SCM1-DC has external SM1 (1.035"-40) threading, and Thorlabs offers a line of microscope camera port adapters that will allow the 2SCM1-DC to be installed on many commercial microscopes. The 2SCM1-DC places the cameras at a distance of 4.04" to 4.16" (102.6 mm to 105.6 mm) from the camera port, which may be outside the parfocal distance of some microscopes, including inverted microscopes from Nikon and Olympus. For these microscopes, the 2SCM1-DC mount can be used if parfocality with the eyepieces is not needed.

The 2SCM1-DC mount will be parfocal with Thorlabs' Cerna® Series Microscopes and upright microscopes from Nikon and Olympus. For Olympus BX microscopes, Thorlabs offers the SM1A51 camera port adapter with SM1 threading. For both Cerna Series Microscopes and upright microscopes from Nikon, the 2SCM1-DC can be attached to the trinoc camera port using our SM1A58 camera port adapter.

Turnkey prealigned systems with cameras and three-way camera mounts are also available as custom items. Please contact ImagingSales@thorlabs.com for more details.

Thorcam 2-Camera Overlay Plugin
Click to Enlarge

Screenshot of ThorCam Overlay Plug-in
Scientific Camera Selection Guide
1.4 MP CCD
4 MP CCD
8 MP CCD
200 Frames Per Second, VGA CCD

Compatible with Thorlabs' Scientific Cameras

  • 1.4, 4, or 8 Megapixel as well as Fast Frame Rate Cameras Available
  • Low Read Noise
  • Asynchronous, Triggered, and Bulb Exposure Modes
  • ThorCam GUI included
  • Third-Party Software Support Including LabVIEW, MATLAB, µManager, and Metamorph

Thorlabs' Monochrome Scientific CCD cameras are based on high quantum efficiency, low-noise CCD imagers, which make them ideal for multispectral imaging, fluorescence microscopy, and other high-performance imaging techniques. Both non-cooled and cooled versions are available. For more details about our camera families, please click on the links in the table to the right.

The ThorCam user interface provided with our scientific cameras includes a plug-in to allow for multiple live camera images to be overlaid into a real-time 2-channel composite, eliminating the need for frequent updates of a static overlay image. This live imaging method is ideal for applications such as calcium ratio imaging and electrophysiology. As seen to the left, the plug-in controls image threshold and opacity (Alpha) settings, and can apply false color to one of the two monochrome channels.

Simultaneous NIR and Fluorescence Imaging
Click to Enlarge
Example Setup for Simultaneous NIR/DIC and Fluorescence Imaging

Live Dual-Channel Imaging

Many life science imaging experiments require a cell sample to be tested and imaged under varying experimental conditions over a significant period of time. One common technique to monitor complex cell dynamics in these experiments uses fluorophores to identify relevant cells within a sample, while simultaneously using NIR or differential interference contrast (DIC) microscopy to probe individual cells. Registering the two microscopy images to monitor changing conditions can be a difficult and frustrating task. 

Live overlay imaging allows both images comprising the composite to be updated in real-time versus other methods that use a static image with a real-time overlay. Overlays with static images require frequent updates of the static image due to drift in the system or sample, or due to repositioning of the sample. Live overlay imaging removes that dependency by providing live streaming in both channels. 

Using the ThorCam overlay plug-in with the two-way camera microscope mount, users can generate real-time two-channel composite images with live streaming updates from both camera channels, eliminating the need for frequent updates of a static overlay image. This live imaging method is ideal for applications such as calcium ratio imaging and electrophysiology. 

Example Images

Simultaneous Fluorescence and DIC Imaging

The image sequence below shows mouse kidney cells imaged using a dichroic filter to separate the fluorescence and DIC signals into different cameras. These images are then combined into a two-channel composite live image with false color fluorescence by the ThorCam overlay plug-in.

Fluorescence Image
Fluorescence Image of Two Camera Overlay
Click to Enlarge
2-Channel Composite Live Image
Image Overlay from Two Cameras
Click to Enlarge
Microaspiration Image using Two Cameras
Click to Enlarge
In the image above, the pipette is visible in the DIC image as two lines near the center of the frame.

Microaspiration Using a Micropipette

The image to the right shows a live, simultaneous overlay of fluorescence and DIC images. The experiment consists of a microaspiration technique using a micropipette to isolate a single neuron that expresses GFP. This neuron can then be used for PCR. This image was taken with our 1.4 Megapixel Cameras and our 2SCM1-DC Two-Camera Mount and shows the live overlay of fluorescence and DIC from the ThorCam plug-in. Image courtesy of Ain Chung, in collaboration with Dr. Andre Fenton at NYU and Dr. Juan Marcos Alarcon at The Robert F. Furchgott Center for Neural and Behavioral Science, Department of Pathology, SUNY Downstate Medical Center.

Simultaneous NIR Dodt Contrast and Epi Fluorescence imaging

The image to the right shows a live, simultaneous overlay of fluorescence and near-infrared Dodt contrast images of a 50 µm brain section from a CX3CR1-GFP mouse, which has been immunostained for PECAM-1 with Alexa-687 to highlight vasculature. The Dodt contrast uses a quarter annulus and a diffuser to create a gradient of light across the sample that can reveal the structure of thick samples. The image was taken with our Scientific Cameras and our 2SCM1-DC Two-Camera Mount. Sample courtesy of Dr. Andrew Chojnacki, Department of Physiology and Pharmacology, Live Cell Imaging Facility, Snyder Institute for Chronic Diseases, University of Calgary.

Other Configurations

The 2SCM1-DC two-camera mount can also be utilized with other optics sharing the same 25 mm x 36 mm x 1 mm size of a standard dichroic mirror. The schematic to the left below suggest a two-camera imaging system designed for dual wavelength fluorescence imaging with a dichroic mirror. In the right schematic, a 50:50 plate beamsplitter can be used to split the signal evenly; this is useful, for example, for imaging the sample using two different cameras simultaneously.

Simultaneous Dual Wavelength Fluorescence using a 50:50 Beamsplitter
Click to Enlarge

Example Setup for Dual Wavelength Fluorescence Imaging Using a 50:50 Plate Beamsplitter
Simultaneous Dual Wavelength Fluorescence Imaging
Click to Enlarge
Example Setup for Dual Wavelength Fluorescence Imaging Using a Dichroic Mirror

Posted Comments:
christian  (posted 2017-12-07 17:13:27.933)
Is there a way to attach 2 cameras to a microscope, being able to select camera 1 or camera 2 with a motorized 100% flipper mirror?
YLohia  (posted 2018-03-26 10:14:37.0)
Hello, thank your for contacting Thorlabs. Unfortunately, we currently do not have such a fixture. Your best bet would be looking into MFF101/MFF102, but these will not allow for a light-tight system. Please note that the maximum load capacity is 120g.
maoyafikuddin  (posted 2017-06-21 04:38:53.183)
We are trying to perform multiple fluorescence imaging in our existing Nikon inverted microscope of model TE 2000 U. For this purpose, we need a multi port camera attachment which will enable us to simultaneous image capturing as well as video recording on two camera. Therefore, requesting for availability of following components: 1. Multi port camera attachment preferably two or three 2. Attachment of fluorescence filters between the camera and camera port which is C mount. 3. A suitable model of scientific CMOS camera having resolution 1024×1024 pixel and fps of min. 40 FPS at full resolution. Must be compiled with synchronisation facility. Any other solutions or modification to the above said system will be highly appreciated.
tfrisch  (posted 2017-07-20 01:24:31.0)
Hello, thank you for contacting Thorlabs. It looks like you are already in contact with our Scientific Imaging Team about potential solutions for using this with an inverted scope.

Two-Camera Mount for Upright Microscopes

Based on your currency / country selection, your order will ship from Newton, New Jersey  
+1 Qty Docs Part Number - Universal Price Available
2SCM1-DC Support Documentation
2SCM1-DCTwo-Camera Mount for Upright Microscopes
$1,854.00
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Additional Dichroic Filter Inserts


Click to Enlarge

Each filter cube insert can hold a fluorescence filter set: one dichroic mirror, one excitation filter, and one emission filter.

Additional DFM1T1 inserts allow for different filters to be swapped in and out of our 2SCM1-DC two camera mount. The kinematic design creates a repeatable alignment stop so that the system will not require realignment after the inserts are switched. The image to the right shows a fluorescence filter set being installed in the DFM1T1.

Based on your currency / country selection, your order will ship from Newton, New Jersey  
+1 Qty Docs Part Number - Universal Price Available
DFM1T1 Support Documentation
DFM1T1Kinematic Cage Cube Top for Fluorescence Filter Sets, Right-Turning
$262.65
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