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Microscope Immersion Oils
Fluorescence Image of Buttercup Root Taken with Immersion Oil Using a 40X, 1.3 NA Objective
Olympus Type F Immersion Oil
Cargille Type LDF Immersion Oil
Leica Type N Immersion Oil
These Microscope Immersion Oils are designed for use with Oil Immersion Microscope Objectives. Placing an oil medium between the front surface of the objective and the cover glass allows the objective to achieve a high numerical aperture, maximizing light collection by the objective. To minimize refraction of light from the sample, the refractive index of each immersion oil is very close to those of cover glass.
Immersion oils are available with low or very low autofluorescence. Autofluorescence is the natural fluorescence emission of the oil when exposed to light. Each immersion oil has a different level of background emission, which either increases or decreases the contrast of the image; oils with very low autofluorescence are optimized for use in sensitive or UV fluorescence microscopy applications.
To clean immersion objectives after use with immersion oils, use a soft optical cleaning tissue such as our MC-5 premium lens tissues.