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TIDE™ Whole-Slide-Scanning Research Microscope![]()
![]() Please Wait ![]() Click Here for Enlarged View of Whole-Slide Image Click Here for Enlarged View of Inset A fluorescence image of bovine pulmonary artery endothelial (BPAE) cells. The mitochondria were stained with MitoTracker® Red CMXRos, the f-actin was stained with Alexa Fluor® 488 phalloidin, and the nuclei were counterstained with DAPI. The image (scan area: 15 mm x 15 mm) was taken at 31Xa magnification. The inset view shows a small area (604 µm x 627 µm) of the whole-slide image to illustrate the clarity and level of detail. ![]() Click Here for Enlarged View of Whole-Slide Image Click Here for Enlarged View of Inset A color image of a sample stained with DAB and counterstained with hematoxylin. The image (scan area: 20.5 mm x 21.5 mm) was taken at 31Xa magnification. The inset view shows a small area (1.3 mm x 1.27 mm) of the whole-slide image to illustrate the clarity and level of detail. Features
Scientific Challenge Solution The patented dynamic autofocus feature of TIDE adjusts for variations in the slide and sample. Once the whole-slide scan is complete, regions of interest can be drawn on the slide image displayed in the TIDE GUI. The stage can be moved to these regions for more detailed study with the fully featured Nikon Eclipse Ti-E microscope. Thorlabs' TIDE is available in three configurations: fluorescence imaging, brightfield imaging, or both. Advantages
TIDE System PricingThe pricing shown below is base pricing and does not include the cost of light sources, optics (such as objectives and fluorescence filter sets), and the PC required for operation. Our sales team is available to discuss your specific imaging requirements and will provide a quote for an optimized system. The large datasets generated by TIDE require careful selection of a computer; therefore the PC must be purchased from Thorlabs. In certain circumstances, we can incorporate customer-supplied optics. This system is for research applications only. ![]() Click Here for Enlarged View of Whole-Slide Image Click Here for Enlarged View of Inset Image of rat brain sections stained with Thionine (scan area: 63.4 mm x 45 mm) imaged at 31X magnification*. The inset shows a small area (448 µm x 315 µm) of the whole slide image. Sample from NeuroScience Associates. ![]() Click Here for Enlarged View of Whole-Slide Image Click Here for Enlarged View of Inset A merged dual-fluorescence image (scan area 6 mm x 9.4 mm) of a mouse kidney stained with Alexa Fluor® 488 WGA, Alexa Fluor® 568 Phalloidin, and DAPI imaged at 31X magnification*. The inset shows a small area (607 µm x 628 µm) of the whole slide image. *Magnification Calculated Using DICOM Standard with 20X Objective SpeedThe advantages of our TIDE™ systems increase as magnification increases. By taking advantage of the scanning stage's control-loop feedback, the Thorlabs TIDE systems produce images with high positional accuracy at significantly faster speeds than stop-and-stare imaging methods. Additionally, the proprietary autofocus monitors the sample in real-time, removing focus map generation from the workflow. Some typical examples of slide scan times using a standard stop-and-stare system versus Thorlabs' TIDE are outlined in the table below.
Higher-Quality, Large-Format ImagesThorlabs' TIDE systems, incorporating our patented object scanning charge accumulated readout technology, are ideal for applications where positional accuracy is paramount. The integration of the camera into the control loop of the scanning stage allows for precise image registration on the pixel level while capitalizing on the speed of the stage. This unique implementation can provide images with better positional information than standard stop-and-stare methods. For more details on our scanning technology, see the How It Works tab. High positional accuracy is achieved by using the encoder of the stage to tie the rows of pixels to absolute positions in the resulting images, which allows these images to be precisely tiled. The resulting image is created based on absolute position without the use of overlapping images and stitching. Conversely, stop-and-stare scanners require the adjacent images to overlap slightly in order to use post-processing to align the images. Furthermore, this overlapping data can be a challenge when analyzing image stacks. Additionally, TIDE allows for large-format imaging with high positional image alignment for samples with sparse features. This is typically a challenge with off-the-shelf stitching programs that rely on feature recognition to align images. The result is one large image without the risk of lost data inherent in many standard stitching schemes. Image OutputThe included TIDE™ LS software GUI provides a user-friendly viewer that allows seamless zooming of the high-resolution images and the ability to select and save regions of interest. In addition to composite large-format images, TIDE LS can also save each high-resolution image in TIFF or JPEG format. These file formats provide full flexibility for any post processing required. The file structure also allows the user to either save the entire data set or only save regions of interest to reduce the amount of data saved and improve ease of sharing results with remote collaborators. For more information on the software and image export options please see the Software tab. A screenshot of Thorlabs' TIDE™ LS software. The whole-slide view of the Tiki Goddess, a trichrome-stained frontal young mouse tissue section, is shown on the left side of the window. Selected regions of interest from the whole slide view are displayed in the main window. Sample courtesy of Dr. George McNamara, MD Anderson Cancer Center, Houston, Texas.
TIDE™ LS Software
View and Manipulate Data from Anywhere Using Your Favorite Software PackageAll image data is saved in a non-proprietary JPEG or TIFF format, leaving the user to view and manipulate the data with any software supporting these formats. Thorlabs' TIDE LS software can also be freely shared on multiple machines, making it easy to review and share images. Export Control for Manageable Data SetsSlide scanning systems allow for large-area, high-resolution image creation. This results in very large file sizes that can be a challenge to manage and work with. The included image exporter allows users to easily export images in a number of ways. Full-resolution data sets can be broken into smaller subsets for analysis; conversely, full-resolution images can be combined into larger images or downsampled for publications.
Custom Tiled ImagesSelecting "Custom Tiled" from the Image Export window allows the user to optimize the size of the individual high-resolution images for either smaller file size or smaller number of images. Selecting a smaller number of larger full-resolution images can often save processing time. In the example shown above, the whole-slide scan is saved as a matrix of 16 x 15 full-resolution images. One of these tiles is shown to the right of the software screenshot. DownsamplingSelecting "Single Image Per Scan" from the Image Export window allows the user to export one single image. This reduces the image size by sampling the image to fit a user-selected size, which is ideal for exporting the whole-slide image for publishing. Natively Open Scans in FijiEach scan can be natively opened from Fiji by simply clicking a button. Users no longer need to create image stacks for export. Click on the TIDE™ Whole-Slide Scanning System Components, shown below, for further details.The image shows an EV103 system configured for both brightfield and fluorescence imaging. Microscope
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Posted Comments: | |
cbrideau
 (posted 2014-01-13 15:00:13.967) Do you guys have a PDF brochure for this incorporating the information and pictures from the Overview, Advantages, and Components tab? sharrell
 (posted 2014-01-14 08:19:44.0) Response from Sean at Thorlabs: Thank you for your interest in our TDI system. This technology was featured in our recent Life Sciences e-catalog. This link will lead to the pdf of the High-Content Screening section; the information about TDI can be found on pages 178 - 185 of the pdf: http://www.thorlabs.com/images/Catalog/Imaging/3_High%20Content%20Imaging.pdf. The entire catalog, or other sections, may be downloaded here: http://www.thorlabs.com/support.cfm and clicking on the "Download Catalog" tab. |
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