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Cerna® Mini Microscopes


  • Quick and Easy Installation
  • Ideal for Sample Preparation, Imaging, and Inspection
  • 6" Throat Depth Accommodates Large Samples
  • Easily Adaptable to Changing Imaging Needs

SFMGFP2

The Cerna® Mini Microscope uses an open-frame design to maximize space for experiments.


Mouse Kidney Cells Labeled with Alexa Fluor 488® Wheat Germ Agglutinin Imaged with the SFMGFP

Rigid Sample Holder Stand and Optical Table Not Included

Related Items


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Mouse Kidney
Click for Full Size Image
449 µm x 335.5 µm FOV with 1.4 MP Camera
mouse kidney
Click for Full Size Image
906.5 µm x 680 µm FOV with 8 MP Camera
Images of mouse kidney cells labeled with Alexa Fluor® 488 Wheat Germ Agglutinin taken with the SFMGFP. The image on the left was taken with the 1.4 Megapixel Scientific Camera included with the SFMGFP. For the image on the right, the camera was replaced with an 8 Megapixel Scientific Camera.
Simple Fluorescence Microscope
Click to Enlarge

The objective height of the Cerna mini microscope can be manually adjusted over a range of 6.38". A motorized objective holder provides 1" of fine Z focus control.

Features

  • Cerna® Mini Microscopes for Widefield or Fluorescence Imaging
  • User Configurable Versions Allow for User-Supplied Components
  • Preconfigured Versions Ready for Imaging Right Out of the Box
  • Accepts C-Mount Cameras from Thorlabs and Other Major Manufacturers
  • 6" Throat Depth and Open Frame Design Maximizes Space for Large Samples
  • 1" of Fine Motorized Motion in X, Y, and Z
  • Objective Nosepiece has M32 x 0.75 Threads
    • Adapter for M25 x 0.75 Objective Threads Included
  • Easily Integrate Custom Modules to Microscope's XT66 Rail Body

Thorlabs' Cerna Mini Microscopes are designed to support widefield, widefield fluorescence, or reflected light imaging. They are quick to set up and simple to use. We offer user configurable microscopes, which are the basic system with no add-ons included, as well as preconfigured microscopes, which are fully outfitted for imaging right out of the box.

There are four types of Cerna mini microscopes. User configurable microscopes, Item #'s SFM2 and SFM, are designed for users to integrate their own scientific camera, objective, illumination source, fluorescence filter set, and filter cube. The SFM2 supports widefield imaging, while the SFM, which includes an epi-illuminator module, supports epi-fluorescence imaging. The SFMGFP and SFMGFP2 microscopes are our preconfigured microscopes. They are designed for epi-fluorescence imaging of Green Fluorescence Protein (GFP) right out of box and include a 470 nm LED with SFM80 collimation module, fluorescence filter set for GFP, and 10X objective. The SFMGFP and SFMGFP2 microscope systems differ with respect to the included camera; the SFMGFP comes with a 1.4 megapixel scientific CCD camera and the SFMGFP2 includes the 2.1 megapixel sCMOS scientific camera.

Each microscope has a deep 6" throat depth and slim 3" wide design to provide ample space around the objective for adding sample stages, perfusion equipment, or other experimental apparatus. The motorized stepper motor stage can move the entire microscope 1" in X and Y. The nosepiece height can be manually adjusted to provide up to 16.70" of clearance for large samples, as shown in the photos to the right. The objective holder has up to 1" of motorized focusing adjustment.

These microscopes are compatible with all of Thorlabs' scientific cameras because each is equipped with a 1X camera tube with C-Mount (1.00"-32) threads. The camera port also features 4.1 mm of focus adjustment, which can be easily set by turning the silver knurled ring at the top of the camera tube.

The epi-illuminator module, included with all microscopes except the SFM2, accepts one of two collimation modules (available below) designed for Thorlabs' mounted LEDs, and can be easily modified to accept two collimation modules by replacing the epi-illuminator spacer block at the rear of the unit with a DFM1(/M) filter cube, C4W-CC cage cube connector, and an SM1CP2 end cap.

In order to minimize the footprint on the table and maximize the throat depth, the base of these microscopes must be bolted to an optical table or breadboard to remain upright. The microscope can be mounted to the MB1224 (MB3060/M) 12" x 24" (300 mm x 600 mm) breadboard to create a free-standing, portable system.

Accessories and Upgrades
To allow the experimenter to use a single microscope for samples that require different imaging configurations, these microscopes feature a modular design that allows light sources, filter cubes, cameras, and eyepieces to be easily added or exchanged. This design allows one Cerna mini microscope to be adapted for many applications from patch clamping to fluorescence imaging in live samples, all while taking up minimal space in the lab. Compatible modules are offered separately below.

Thorlabs' rigid stands (available separately) can be used to position microscope slides, well plates, petri dishes, micromanipulators, and other experimental apparatus underneath the objective, allowing the microscope to be used for both in vivo and in vitro imaging applications. The stands are easy to install and remove, allowing the microscope to be quickly adapted from imaging slices to examining intact samples.

DIY Customization
For the DIY builder, the body of each Cerna mini microscope uses a section of 66 mm rail and a Ø1.5" optical post, allowing users to integrate custom modules built from our extensive catalog of optomechanics. Microscopes with an epi-illuminator module have four 4-40 taps for compatibility with our 30 mm cage system, supporting the integration of custom illumination sources. We also offer several adapters (available below) that combine a D1N dovetail with standard cage system and lens tube mounting features for users interested in adding custom camera ports or additional epi-illuminator modules. Additionally, we have trinocular port adapters, designed to allow trinoculars to be used with DIY Cerna systems. For integrating a transmitted light module, we have our 66 mm rail carriage, optomechanical mounts, and mounted LEDs.

Microscope Body
Rail Length  174.3 mm (6.86")
Post Length 200 mm (7.87")
Throat Depth 6.00" (152.4 mm)
Manual Height Adjustment 6.38" (161.9 mm)
Motorized Motion 1" Microscope Travel in X and Y
1" Z Motion of Objective Stage
Nosepiece Threading Internal M32 x 0.75,
M32M25S Included for M25 x 0.75 Threaded Objectives
Mounting Screws 1/4"-20 or M6, 8 of Each Type Included
Breadboard (Not Included) MB1224 12" x 24" Breadboard or
MB3060/M 300 mm x 600 mm Breadboard
Camera Port
Item # WFA4100
Magnificationa 1X
Dovetail Male D1N
Tube Lens Focal Lengtha 200 mm
Camera Threading C-Mount (1.00"-32)
  • Please see the Magnification & FOV tab for more information on how an objective's magnification is affected by the system's tube lens.
Motorized Translation Stages (XYZ Motion)a
Translation Range 25.4 mm (1")
Bidirectional Repeatability 1 μm
Backlash 1 μm
Minimum Incremental Movement 100 nm
Minimum Achievable Repeatable Movement 200 nm
Velocity (Max) 7 mm/s
Acceleration (Max) 11 mm/s²
Cable Length 6' (1.8 m)
Load Capacitya
Stepper Motor Specifications
  • The motor used to translate the microscope body in X and Y is the same motor as the one used to translate the microscope objective along the Z axis. Therefore the load capacity specifications are defined for the motor oriented horizontally and vertically, as indicated.
  • Without the stages, the SFMGFP weighs 16.3 lbs (7.4 kg) and the SFM weighs 14.8 lbs (6.7 kg). These values were used to calculate the load capacity available for mounting additional components on the microscope body.
Motion Controller
Item # MCM3001
Motor Output
Motor Drive Voltage 24 V
Motor Drive Current 7.0 A (Peak)
3.0 A (RMS)
Motor Drive Type 12-Bit PWM Control
Control Algorithm Open-Loop Microstepping
Stepping 64 Microsteps per Full Step
Encoder Resolution 0.212 µm
Total Steps per Revolution 12,800
Maximum Stepping Velocity 4577 steps/s
Position Feedback Quadrature Encoder (QEP) Input, 5 V
Encoder Feedback Bandwidth 16 MHz
Position Counter 32 Bit
Operating Modes Position and Velocity
Velocity Profile Trapezoid
Motor Drive Connector
Input Power Requirements
General
LED Collimation Package (Only Included with SFMGFP/SFMGFP2)
Item # SFM80
Compatible LED Divergence Angles 80° to 120°
Operating Wavelength Range 350 to 700 nm
LED (Only Included with SFMGFP/SFMGFP2)
Spectrum (Click for Graph) info
Color Blue
Nominal Wavelength 470 nm
LED Power Output at Max Current 650 mW (Min)
710 mW (Typical)
Maximum Current (CW) 1000 mA
Forward Voltage 3.2 V
Bandwidth (FWHM) 25 nm
Irradiance (Typical) 21.9 µW/mm²
Electrical Power 3.200 W
Viewing Angle (Full Angle at Half Maximum) 80°
Emitter Size 1 mm x 1 mm
Typical Lifetime 10,000 Hours
T-Cube™ LED Driver (Only Included with SFMGFP/SFMGFP2)
Item # LEDD1B (LED Driver)
KPS101 (Power Supply)
General
Output Current Range 0 to 1200 mA
LED Current Limit Set Point Range 200 to 1200 mA
LED Forward Voltage 11 V (Min)
12 V (Typical)
Current Ripple 8 mA
Current Ripple Frequency 570 kHz
Modulation Modea
Trigger Modea
General Data
  • All technical data is valid at 23 ± 5 °C and 45 ± 15% relative humidity.
  • Specifications for the modulation and trigger modes depend on the forward voltage and capacitance of the LED.
Fluorescence Filter Set (Only Included with SFMGFP/SFMGFP2)
Item # MDF-GFP
Design Fluorophore Green Fluorescent Protein (GFP) / Alexa Fluor® 488
Filter Type Excitation Filter Emission Filter Dichroic
Size Ø25 ± 0.1 mm Ø25 ± 0.1 mm 25.0 mm x 36.0 mm
Clear Aperture >Ø21 mm >(22.5 mm x 32.4 mm)
Angle of Incidence 0° ± 5° 45° ± 1.5°
Thickness 5.0 ± 1.0 mm 3.5 ± 0.1 mm 1.0 ± 0.1 mm
Surface Quality 60-40 Scratch Dig
Substrate Fused Silica
Center Wavelength 469 nm 525 nm -
FWHM 35 nm 39 nm -
Reflection Band - -  452 to 490 nm (Ravg > 90%)
Transmission Band - - 505 to 800 nm (Tabs > 90%)
AR Coatinga - - 400 to 800 nm (Ravg < 2%)
Performance Graphs
(Click Icon for Details)
info
  • Designed for 45° AOI on the back surface.

Objective Dimensions

Objective Dimensions

Objective (Only Included with SFMGFP/SFMGFP2)
Item # N10X-PF
Magnification 10X
Manufacturer Part # MRH00101
Numerical Aperture (NA) 0.30
Working Distance (WD)a 16 mm
Parfocal Lengtha 60 mm
Design Tube Lens Focal Length 200 mm
Coverslip Correction 0.17 mm
Barrel Diametera 30.0 mm
Lengtha 48.7 mm
Threading M25 x 0.75 External Threads
  • These dimensions are defined in the drawing to the right.
Scientific Cameraa (Only Included with SFMGFP/SFMGFP2)
System SFMGFP SFMGFP2
Item # 1501M-GE CS2100M-USB
Sensor Sony ICX285AL
Monochrome CCD (Grade 0)
Monochrome sCMOS
Effective Number of Pixels (Horizontal x Vertical) 1392 x 1040 1920 x 1080
Imaging Area (Horizontal x Vertical) 8.98 mm x 6.71 mm 9.68 mm x 5.44 mm
Pixel Size 6.45 μm x 6.45 μm 5.04 µm x 5.04 µm
Optical Format 2/3" Format (11 mm Diagonal) 2/3" Format (11 mm Diagonal)
Peak Quantum Efficiency 60% at 500 nm 61% at 600 nm
Quantum Efficiency (Click for Graph)
Click for Data

Click for Data
IR-Blocking Filter Transmission
(Click for Graph)

Click for Data
N/A
Exposure Time 0 to 1000 seconds in 1 ms Incrementsb 0.029 ms to 7767.2 ms in ~0.03 ms Increments
Pixel Clock Speed 20 MHz or 40 MHz 74.25 MHz to 148.5 MHz
Analogue-to-Digital Converter Resolution N/A 16 Bit
Analog-to-Digital Converter Gain 0 to 1023 Steps (0.036 dB/Step) N/A
Optical Black Clamp 0 to 1023 Steps (0.25 ADU/Step)c N/A
Vertical Hardware Binning Continuous Integer Values from 1 to 24d Continuous Integer Values from 1 to 16e
Horizontal Software Binning Continuous Integer Values from 1 to 24d Continuous Integer Values from 1 to 16e
Region of Interest 1 x 1 Pixel to 1392 x 1040 Pixels, Rectangular 8 x 2 Pixelse to 1920 x 1080 Pixels, Rectangular
Read Noise <7 e- at 20 MHzf <1 e- Mediang
Digital Output 14 Bit 16 Bit
Host PC Interface PCIe x 1 Slot USB 3.0
Lens Mount C-Mount (1.000"-32) C-Mount (1.000"-32)
  • The specified performance is valid when using a computer with the specifications listed in the Recommended System Requirements table in the Camera Software tab above.
  • Exposure time varies with operating mode; exposure times shorter than 1 ms may be possible when using an external trigger.
  • ADU = Analog-to-Digital Unit
  • Camera Frame Rate is impacted by the Vertical Hardware Binning Parameter.
  • When Binning at 1 x 1
  • If your application is read-noise limited, we recommend using the lower CCD pixel clock speed of 20 MHz. For more information about read noise, and for examples of how to calculate the limiting factor of total camera noise, please see the Camera Noise tab.
  • As specified by the sensor manufacturer. The RMS read noise is <1.5 e.-

ThorCam™

ThorCam is a powerful image acquisition software package that is designed for use with our cameras on 32- and 64-bit Windows® 7 or 10 systems. This intuitive, easy-to-use graphical interface provides camera control as well as the ability to acquire and play back images. Single image capture and image sequences are supported. Please refer to the screenshots below for an overview of the software's basic functionality.

Application programming interfaces (APIs) and a software development kit (SDK) are included for the development of custom applications by OEMs and developers. The SDK provides easy integration with a wide variety of programming languages, such as C, C++, C#, Python, and Visual Basic .NET. Support for third-party software packages, such as LabVIEW, MATLAB, and µManager* is available. We also offer example Arduino code for integration with our TSI-IOBOB2 Interconnect Break-Out Board.

*µManager control of Zelux and 1.3 MP Kiralux cameras is not currently supported. When controlling the Kiralux Polarization-Sensitive Camera using µManager, only intensity images can be taken; the ThorCam software is required to produce images with polarization information.

Recommended System Requirementsa
Operating System Windows® 7 or 10 (64 Bit)
Processor (CPU)b ≥3.0 GHz Intel Core (i5 or Higher)
Memory (RAM) ≥8 GB
Hard Drivec ≥500 GB (SATA) Solid State Drive (SSD)
Graphics Cardd Dedicated Adapter with ≥256 MB RAM
Motherboard USB 3.0 (-USB) Cameras: Integrated Intel USB 3.0 Controller
or One Unused PCIe x1 Slot (for Item # USB3-PCIE)
GigE (-GE) Cameras: One Unused PCIe x1 Slot
Connectivity USB or Internet Connectivity for Driver Installation
  • See the Performance Considerations section below for recommendations to minimize dropped frames for demanding applications.
  • Intel Core i3 processors and mobile versions of Intel processors may not satisfy the requirements.
  • We recommend a solid state drive (SSD) for reliable streaming to disk during image sequence storage.
  • On-board/integrated graphics solutions present on Intel Core i5 and i7 processors are also acceptable.

Software

Version 3.5.1

Click the button below to visit the ThorCam software page.

Software Download

Example Arduino Code for TSI-IOBOB2 Board

Click the button below to visit the download page for the sample Arduino programs for the TSI-IOBOB2 Shield for Arduino. Three sample programs are offered:

  • Trigger the Camera at a Rate of 1 Hz
  • Trigger the Camera at the Fastest Possible Rate
  • Use the Direct AVR Port Mappings from the Arduino to Monitor Camera State and Trigger Acquisition
Software Download

Click the Highlighted Regions to Explore ThorCam Features

Thorcam GUI Window

Camera Control and Image Acquisition

Camera Control and Image Acquisition functions are carried out through the icons along the top of the window, highlighted in orange in the image above. Camera parameters may be set in the popup window that appears upon clicking on the Tools icon. The Snapshot button allows a single image to be acquired using the current camera settings.

The Start and Stop capture buttons begin image capture according to the camera settings, including triggered imaging.

Timed Series and Review of Image Series

The Timed Series control, shown in Figure 1, allows time-lapse images to be recorded. Simply set the total number of images and the time delay in between captures. The output will be saved in a multi-page TIFF file in order to preserve the high-precision, unaltered image data. Controls within ThorCam allow the user to play the sequence of images or step through them frame by frame.

Measurement and Annotation

As shown in the yellow highlighted regions in the image above, ThorCam has a number of built-in annotation and measurement functions to help analyze images after they have been acquired. Lines, rectangles, circles, and freehand shapes can be drawn on the image. Text can be entered to annotate marked locations. A measurement mode allows the user to determine the distance between points of interest.

The features in the red, green, and blue highlighted regions of the image above can be used to display information about both live and captured images.

ThorCam also features a tally counter that allows the user to mark points of interest in the image and tally the number of points marked (see Figure 2). A crosshair target that is locked to the center of the image can be enabled to provide a point of reference.

Third-Party Applications and Support

ThorCam is bundled with support for third-party software packages such as LabVIEW, MATLAB, and .NET. Both 32- and 64-bit versions of LabVIEW and MATLAB are supported. A full-featured and well-documented API, included with our cameras, makes it convenient to develop fully customized applications in an efficient manner, while also providing the ability to migrate through our product line without having to rewrite an application.

Thorcam Software Screenshot
Click to Enlarge

Figure 1: A timed series of 10 images taken at 1 second intervals is saved as a multipage TIFF.
Thorcam Software Screenshot
Click to Enlarge

Figure 2: A screenshot of the ThorCam software showing some of the analysis and annotation features. The Tally function was used to mark four locations in the image. A blue crosshair target is enabled and locked to the center of the image to provide a point of reference.

 

Performance Considerations

Please note that system performance limitations can lead to "dropped frames" when image sequences are saved to the disk. The ability of the host system to keep up with the camera's output data stream is dependent on multiple aspects of the host system. Note that the use of a USB hub may impact performance. A dedicated connection to the PC is preferred. USB 2.0 connections are not supported.

First, it is important to distinguish between the frame rate of the camera and the ability of the host computer to keep up with the task of displaying images or streaming to the disk without dropping frames. The frame rate of the camera is a function of exposure and readout (e.g. clock, ROI) parameters. Based on the acquisition parameters chosen by the user, the camera timing emulates a digital counter that will generate a certain number of frames per second. When displaying images, this data is handled by the graphics system of the computer; when saving images and movies, this data is streamed to disk. If the hard drive is not fast enough, this will result in dropped frames.

One solution to this problem is to ensure that a solid state drive (SSD) is used. This usually resolves the issue if the other specifications of the PC are sufficient. Note that the write speed of the SSD must be sufficient to handle the data throughput.

Larger format images at higher frame rates sometimes require additional speed. In these cases users can consider implementing a RAID0 configuration using multiple SSDs or setting up a RAM drive. While the latter option limits the storage space to the RAM on the PC, this is the fastest option available. ImDisk is one example of a free RAM disk software package. It is important to note that RAM drives use volatile memory. Hence it is critical to ensure that the data is moved from the RAM drive to a physical hard drive before restarting or shutting down the computer to avoid data loss.

Pixel PeekVertical and Horizontal Line ProfilesHistogramCamera Control IconsMeasurement and Annotation FunctionsMeasurement and Annotation Functions
Simple Fluorescence Microscopes Shipping List
Item # SFM2 SFM SFMGFP SFMGFP2
Microscope Body with PLS-XY Stage and Motorized Objective Mount 1 1 1 1
3-Axis Controller with Knob Box (MCM3001) 1 1 1 1
1X Camera Port with 200 mm Focal Length Tube Lens (WFA4100) 1 1 1 1
Epi-Illuminator Cube Housing (WFA2002) - 1 1 1
OEM Microscopy Filter Cube (MDFM-MF2) - - 1 1
GFP/Alexa Fluor 488 Filter Set (MDF-GFP) - - 1 1
T-Cube™ LED Driver (LEDD1B) - - 1 1
T-Cube™ Power Supply (KPS101) - - 1 1
Blue (470 nm) LED - - 1 1
80° Collimating Tube with XY Adjustment (SFM80) - - 1 1
Monochrome Gig-E Scientific CCD Camera (1501M-GE) - - 1 -
Monochrome UBS 3.0 sCMOS Camera (CS2100M-USB) - - - 1
10X Nikon Plan Fluorite Imaging Objective (N10X-PF) - - 1 1
M6 Socket Head Cap Screw 8 6 8 8
1/4"-20 Socket Head Cap Screw 8 6 8 8
M6 Flat Washer 8 6 8 8
LED Driver Operating Manual - - 1 1
Thorlabs Digital Camera Quick Start Guide - - 1 1
ThorCam Camera Software CD - - 1 1
Widefield Viewing Optical Path
When viewing an image with a camera, the system magnification is the product of the objective and camera tube magnifications. When viewing an image with trinoculars, the system magnification is the product of the objective and eyepiece magnifications.
Magnification & FOV Calculator
Manufacturer Tube Lens
Focal Length
Leica f = 200 mm
Mitutoyo f = 200 mm
Nikon f = 200 mm
Olympus f = 180 mm
Thorlabs f = 200 mm
Zeiss f = 165 mm

The rows highlighted in green denote manufacturers that do not use f = 200 mm tube lenses.

Magnification and Sample Area Calculations

Magnification

The magnification of a system is the multiplicative product of the magnification of each optical element in the system. Optical elements that produce magnification include objectives, camera tubes, and trinocular eyepieces, as shown in the drawing to the right. It is important to note that the magnification quoted in these products' specifications is usually only valid when all optical elements are made by the same manufacturer. If this is not the case, then the magnification of the system can still be calculated, but an effective objective magnification should be calculated first, as described below.

To adapt the examples shown here to your own microscope, please use our Magnification and FOV Calculator, which is available for download by clicking on the red button above. Note the calculator is an Excel spreadsheet that uses macros. In order to use the calculator, macros must be enabled. To enable macros, click the "Enable Content" button in the yellow message bar upon opening the file.

Example 1: Camera Magnification
When imaging a sample with a camera, the image is magnified by the objective and the camera tube. If using a 20X Nikon objective and a 0.75X Nikon camera tube, then the image at the camera has 20X × 0.75X = 15X magnification.

Example 2: Trinocular Magnification
When imaging a sample through trinoculars, the image is magnified by the objective and the eyepieces in the trinoculars. If using a 20X Nikon objective and Nikon trinoculars with 10X eyepieces, then the image at the eyepieces has 20X × 10X = 200X magnification. Note that the image at the eyepieces does not pass through the camera tube, as shown by the drawing to the right.

Using an Objective with a Microscope from a Different Manufacturer

Magnification is not a fundamental value: it is a derived value, calculated by assuming a specific tube lens focal length. Each microscope manufacturer has adopted a different focal length for their tube lens, as shown by the table to the right. Hence, when combining optical elements from different manufacturers, it is necessary to calculate an effective magnification for the objective, which is then used to calculate the magnification of the system.

The effective magnification of an objective is given by Equation 1:

Equation 1 (Eq. 1)

Here, the Design Magnification is the magnification printed on the objective, fTube Lens in Microscope is the focal length of the tube lens in the microscope you are using, and fDesign Tube Lens of Objective is the tube lens focal length that the objective manufacturer used to calculate the Design Magnification. These focal lengths are given by the table to the right.

Note that Leica, Mitutoyo, Nikon, and Thorlabs use the same tube lens focal length; if combining elements from any of these manufacturers, no conversion is needed. Once the effective objective magnification is calculated, the magnification of the system can be calculated as before.

Example 3: Trinocular Magnification (Different Manufacturers)
When imaging a sample through trinoculars, the image is magnified by the objective and the eyepieces in the trinoculars. This example will use a 20X Olympus objective and Nikon trinoculars with 10X eyepieces.

Following Equation 1 and the table to the right, we calculate the effective magnification of an Olympus objective in a Nikon microscope:

Equation 2

The effective magnification of the Olympus objective is 22.2X and the trinoculars have 10X eyepieces, so the image at the eyepieces has 22.2X × 10X = 222X magnification.


Image Area on Camera

Sample Area When Imaged on a Camera

When imaging a sample with a camera, the dimensions of the sample area are determined by the dimensions of the camera sensor and the system magnification, as shown by Equation 2.

Equation 5 (Eq. 2)

The camera sensor dimensions can be obtained from the manufacturer, while the system magnification is the multiplicative product of the objective magnification and the camera tube magnification (see Example 1). If needed, the objective magnification can be adjusted as shown in Example 3.

As the magnification increases, the resolution improves, but the field of view also decreases. The dependence of the field of view on magnification is shown in the schematic to the right.

Example 4: Sample Area
The dimensions of the camera sensor in Thorlabs' 1501M-USB Scientific Camera are 8.98 mm × 6.71 mm. If this camera is used with the Nikon objective and trinoculars from Example 1, which have a system magnification of 15X, then the image area is:

Equation 6

Sample Area Examples

The images of a mouse kidney below were all acquired using the same objective and the same camera. However, the camera tubes used were different. Read from left to right, they demonstrate that decreasing the camera tube magnification enlarges the field of view at the expense of the size of the details in the image.

Image with 1X Camera Tube
Click to Enlarge

Acquired with 1X Camera Tube (Item # WFA4100)
Image with 1X Camera Tube
Click to Enlarge

Acquired with 0.75X Camera Tube (Item # WFA4101)
Image with 1X Camera Tube
Click to Enlarge

Acquired with 0.5X Camera Tube (Item # WFA4102)

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User Configurable Cerna® Mini Microscopes 

Simple Fluorescence Microscope
Click to Enlarge

The OSL2 (shown here with the OSL2RFB gooseneck bundle) can be used to illuminate samples viewed with the Cerna Mini Microscope. The SFM2 is shown.
Included Componentsa
SFM2 SFM
Microscope Body with Motorized 2D Translation Stage
1X Camera Tube with 200 mm Focal Length Tube Lens
- Epi-Illuminator Module
Motorized Objective Focusing Module
  • See the Shipping List tab for the itemized list.

Our SFM2 and SFM user configurable microscopes allow the users to customize the microscope with their own illumination source, objective, and scientific camera. These microscopes accept objectives with M32 x 0.75 threads or accept objectives with M25 x 0.75 threads by using the included adapter. Each microscope has a 1X camera tube that features C-Mount threads, making it compatible with any of Thorlabs' scientific cameras. The SFM2 is designed to support widefield imaging, while the SFM includes the epi-illuminator module to support widefield fluorescence and reflected light imaging. The SFM has a complete epi-fluorescence path and users can select their own fluorescence filter set and filter cubes. Fluorescence filter cubes and collimation tubes for Thorlabs' mounted LEDs are provided below. 

Based on your currency / country selection, your order will ship from Newton, New Jersey  
+1 Qty Docs Part Number - Universal Price Available
SFM2 Support Documentation
SFM2Customer Inspired! Cerna Mini Microscope
$9,861.07
Today
SFM Support Documentation
SFMCustomer Inspired! Cerna Mini Microscope with Epi-Illuminator Module
$10,280.12
Lead Time

Preconfigured Cerna® Mini Microscopes for GFP/Alexa Fluor® 488

Fluorescence Microscope on a Breadboard
Click to Enlarge

The SFMGFP2 with a Rigid Stand Slide Holder can be mounted on an MB1224 (MB3060/M) breadboard (not included), shown here with RDF1 rubber mounting feet.
Fluorescence Microscope
Click to Enlarge

The Cerna Mini Microscopes have a slim 3" profile to maximize space for experimental setups.
  • Ready for Epi-Fluorescence Imaging of Green Fluorescent Protein (GFP) Out of the Box
  • Includes SFM System Described Above with the Following Accessories Installed:
    • 470 nm LED with LEDD1B Controller
    • SFM80 Collimation Module
    • MDF-GFP GFP Fluorescence Filter Set in Removable MDFM-MF2 Filter Cube
    • N10X-PF 10X Infinity-Corrected Objective
    • SFMGFP2: CS2100M-USB 2.1 Megapixel sCMOS Camera
    • SFMGFP: 1501M-GE 1.4 Megapixel Scientific CCD Camera
  • 1/4" (M6) Slots to Mount to an Optical Table or Breadboard (Not Included)

The SFMGFP2 and SFMGFP utilize the same optical pathway construction as the SFM above, but are conveniently equipped with everything needed for imaging GFP or Alexa Fluor 488 fluorescence right out of the box. This includes a scientific camera, blue LED, GFP filter set, and 10X objective. These microscopes provide 7.46" to 14.84" between the base of the microscope and the focal plane (including the manual 6.38" adjustment range and 1" of fine focus control). The provided fluorescence filter set and 470 nm LED enable imaging of GFP, Alexa Fluor 488, and other fluorophores of similar excitation/emission wavelengths. Filter sets and mounted LEDs can be easily swapped out to support imaging of other fluorophores or reflected light imaging setups.

These two microscopes differ with respect to the included scientific camera. The SFMGFP2 comes with a 2.1 megapixel sCMOS camera that can be connected to a PC via a USB 3.0 port, while the SFMGFP is equipped with a 1.4 megapixel CCD camera that connects to a PC with gigabit ethernet. The sCMOS camera sensor has low read noise and a large dynamic range, providing the power to resolve dim signals without overexposing robust signals in the same sample. The CCD camera has a software-selectable NIR Enhanced (Boost) mode that generates enough sensitivity up to the 900 - 1000 nm range to acquire usable images for many applications. Both cameras can be operated using the intuitive ThorCam Software package; see the Camera Software tab for details.

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SFMGFP2Cerna Mini Microscope with Epi-Illuminator Module for GFP/Alexa Fluor® 488 with sCMOS Camera
$18,729.13
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LED Collimation Modules (One Required for SFM Microscope)

Simple Fluorescence Microscope
Click to Enlarge

Two SFM80 LED collimation modules connected to the Cerna Mini Microscope with Epi-Illuminator Module using a DFM1(/M), C4W-CC, and SM1CP2.
  • Designed for Thorlabs' Mounted LEDs
  • SFM22 Collimation Module
    • <22° LED Divergence Angle
    • Antireflection-Coated Optics for 400 - 700 nm
  • SFM80 Collimation Module
    • 80° to 120° LED Divergence Angle
    • Antireflection-Coated Optics for 350 - 700 nm

These LED Collimation Modules are designed to allow our mounted LEDs to be connected to Cerna® Mini Microscopes with an Epi-Illuminator Module. Each collimation module connects to an LED via an XY translation mount with ±1 mm of travel, to allow the LED emitter to be centered on the optical axis. The SFM80 is designed for LEDs with an 80° to 120° divergence angle, while SFM22 is for LEDs with a divergence angle of <22°. The table below provides suggested ways to combine each LED mount with compatible mounted LEDs and fluorescence filter sets. Alternatively, the microscope can be set up for reflected light imaging by replacing the fluorescence filter set with a beamsplitter and polarizers. Thorlabs can also customize these modules to work with other LED divergence angles; please contact Tech Support with inquiries.

Each LED collimation module includes two 4-40 cap screws (3/32" hex) and two alignment pins for mounting the module on the epi-illuminator of an SFM, SFMGFP, or SFMGFP2 microscope. To create a two-LED epi-illuminator, replace the epi-illuminator spacer block on the Cerna mini microscope with a DFM1(/M) filter cube, C4W-CC cage cube connector, and an SM1CP2 end cap, as shown in the photo to the right. An SM1T2 lens tube coupler with two locking nuts is included for mounting LEDs that have internal SM1 threading.

One SFM80 is included with the SFMGFP Cerna Mini Microscope with Epi-Illuminator Module for GFP/Alexa Fluor 488.

LED Collimation Module Suggested LEDs Recommended Fluorescence Filter Set
Item # LED Divergence Angle AR Coating Range LED Item # Spectruma Color Nominal Wavelength Filter Set Item # Design Fluorophore Transmissionb
SFM22 <22° 400 - 700 nm M430L4 info Violet 430 nm MDF-CFP CFP info
SFM80 80° to 120° 350 - 700 nm M385L2 info UV 385 nm MDF-BFP BFP info
M395L4 info UV 395 nm
M470L4 info Blue 470 nm MDF-GFP GFP info
MDF-GFP2 GFP / Alexa Fluor® 488 info
M505L4 info Cyan 505 nm MDF-YFP YFP info
M530L4 info Green 530 nm MDF-TOM tdTomato info
  • Click for LED Spectrum and Support Documentation
  • Click for Fluorescence Filter Set Transmission Spectra
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SFM80Customer Inspired! SFM Collimation Module for LEDs with 80° to 120° Divergence Angle
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Filter Cube (Required for SFM Microscope)


Click to Enlarge

These filter cubes connect to the WFA2001C Epi-Illuminator Module Cover (one included with each SFM or SFMGFP microscope).
  • Filter Cubes for Use with SFM, SFMGFP, SFMGFP2 Microscopes
  • MDFM-MF2 OEM Cube Assembly
    • OEM Filter Cube from Olympus
    • Plastic Construction
  • WFA2001C: Extra Epi-Illuminator Module Cover to Aid in Quick Filter Exchanges

This Drop-In Filter Cube holds one Fluorescence Filter Set, which includes an excitation filter, emission filter, and dichroic mirror/beamsplitter. Optics can be mounted, aligned, and swapped out easily, following the videos and assembly instructions below.

The WFA2001C is an extra epi-illuminator module cover, allowing the filter cubes to be pre-mounted for quicker filter exchange.


Installation of Filters into the MDFM-MF2 Cube
Compatible Filter Sizes
Item # MDFM-MF2
Excitation Filter Ø25 mm, Up to 5 mm Thick
Emission Filter Ø25 mm, Up to 3.5 mm Thick
Dichroic Up to 25.2 mm x 36.0 mm x 1.0 mm

Click to Enlarge

MDFM-MF2 Retaining a Dichroic Beamsplitter
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WFA2001C Support Documentation
WFA2001CExtra Filter Cube Cover for WFA2001 and WFA2002 Epi-Illuminator Modules
$188.28
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Optional Upgrade: Inverted-Image Trinoculars

Simple Fluorescence Microscope
Click to Enlarge

A Cerna Mini Microscope with Epi-Illuminator Module Configured with Trinoculars
Trinoculars Drawing
Click for Details

Drawing of WFA4000 and WFA4001 Trinoculars
  • 10X Eyepieces to Observe the FOV with the Naked Eye
  • Available with or without IR Filters for Eye Protection
  • Top-Located Port for Camera Attachment Using 0.7X or 1X Camera Tube (Optional)
  • See the Full Web Presentation for More Information

These Trinoculars produce an inverted image of a sample that can be viewed with the naked eye using the included 10X eyepieces or with a camera connected to the top-located camera port via a camera tube (camera and camera tube sold separately). A lever located on the side of the housing directs the incoming light to either the eyepieces or the camera. In order to protect the viewer's eyes, the WFA4000 trinoculars contain a filter before the eyepieces that blocks NIR light. This filter will not block NIR light that is sent to the camera port and it cannot be removed. To mount the trinoculars onto a Cerna® mini epi-fluorescence microscope, loosen the M4 setscrew at the top of the epi-illuminator module, remove the included camera tube, and replace it with the trinoculars. Tighten the M4 screw to lock the trinoculars in place.

In order to use a C-mount camera with these trinoculars, either the WFA4105 or WFA4106 camera tube is required to position the camera at the image plane. The WFA4105 does not introduce any magnification, while the WFA4106 will produce an image with 0.7 times the magnification provided by the objective. Each camera tube features C-mount threads for mounting scientific cameras and can be secured to the trinoculars via a dovetail and M3 setscrew.

System Magnification
The total system magnification will be the multiplicative product of the objective magnification and the eyepieces or camera tube magnification, depending on which is being used. To achieve an objective's stated magnification, the objective must have been designed for systems using a 200 mm tube lens. Please note that the eyepieces and camera will see a different FOV due to a difference in magnification in each path. Please see the Magnification & FOV tab for more information on objective's magnification dependence on the system's tube lens and how paths with differing magnifications will see a different FOV.

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WFA4000 Support Documentation
WFA4000Trinoculars with 10X Eyepieces, Inverted Image, IR Filter
$3,308.97
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WFA4001 Support Documentation
WFA4001Trinoculars with 10X Eyepieces, Inverted Image, No IR Filter
$1,990.63
5-8 Days
WFA4105 Support Documentation
WFA41051X Camera Tube for WFA4000 & WFA4001 Trinoculars, C-Mount, Male D2N Dovetail
$487.25
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WFA4106 Support Documentation
WFA41060.7X Camera Tube for WFA4000 & WFA4001 Trinoculars, C-Mount, Male D2N Dovetail
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Optional Upgrade: Upright-Image Trinoculars

Simple Fluorescence Microscope
Click to Enlarge

A Cerna Mini Microscope with Epi-Illuminator Module Configured with Trinoculars
Trinoculars Drawing
Click for Details

Drawing of WFA4002 and WFA4003 Trinoculars
Trinoculars Drawing
Click for Details

Drawing of LAURE1 and LAURE2 Trinoculars
  • Ideal for Electrophysiology Applications
  • 10X Eyepieces to Observe the FOV with the Naked Eye
  • Available with or without IR Filters for Eye Protection
  • Top-Located Port for Camera Attachment Using 0.5X, 0.7X or 1X Camera Tube (Optional)
  • See the Full Web Presentation for More Information

These Trinoculars produce an upright image of a sample that can be viewed with the naked eye using the included 10X eyepieces or with a camera connected to the top-located camera port via a camera tube (camera and camera tube sold separately); this configuration is particularly useful for patch-clamping applications. A lever located on the side of the housing directs the incoming light to either the eyepieces or the camera. In order to protect the viewer's eyes, the WFA4000 and LAURE1 trinoculars contain a filter before the eyepieces that blocks NIR light. This filter will not block NIR light that is sent to the camera port and it cannot be removed. To mount the trinoculars onto a Cerna® mini epi-fluorescence microscope, loosen the M4 setscrew at the top of the epi-illuminator module, remove the included camera tube, and replace it with the trinoculars. Tighten the M4 screw to lock the trinoculars in place.

The LAURE1 and LAURE2 trinoculars incorporate several additional features. A red vibration damping knob on the side of the housing allows the detent mechanism on the carriage slider to be disengaged, which is useful for electrophysiology applications that require minimal vibration when switching between the eyepiece and camera port. These trinoculars also include a carriage position indicator switch via a 2.5 mm phono jack, allowing users to connect a laser interlock; it is designed to break the circuit in a laser interlock when the carriage is in the eyepiece output position. For custom-built optical detection systems, these trinoculars have 4-40 taps on the top D2N dovetail connector for compatibility with 30 mm cage systems and 4-40 taps on the bottom D1N dovetail for compatibility with 60 mm cage systems systems.

In order to use a C-mount camera with these trinoculars, a camera tube is required to position the camera at the image plane. The WFA4107, WFA4108, or WFA4109 camera tubes are compatible with the WFA400x trinoculars, while the TC1X camera tube is compatible with the LAUREx trinoculars. The WFA4107 and TC1X camera tubes do not introduce any magnification, while the WFA4108 and WFA4109 will produce images with 0.5 and 0.7 times the magnification provided by the objective, respectively. Each camera tube features C-mount threads for mounting scientific cameras and can be secured to the trinoculars via a dovetail and M3 setscrew.

System Magnification
The total system magnification will be the multiplicative product of the objective magnification and the eyepieces or camera tube magnification, depending on which is being used. To achieve an objective's stated magnification, the objective must have been designed for systems using a 200 mm tube lens. Please note that the eyepieces and camera will see a different FOV due to a difference in magnification in each path. Please see the Magnification & FOV tab for more information on objective's magnification dependence on the system's tube lens and how paths with differing magnifications will see a different FOV.

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LAURE1 Support Documentation
LAURE1Cerna Trinoculars with 10X Eyepieces, Upright Image, IR Filter
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LAURE2Cerna Trinoculars with 10X Eyepieces, Upright Image, No IR Filter
$2,995.00
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WFA4002Trinoculars with 10X Eyepieces, Upright Image, IR Filter
$3,822.32
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WFA4003 Support Documentation
WFA4003Trinoculars with 10X Eyepieces, Upright Image, No IR Filter
$2,496.18
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TC1X Support Documentation
TC1X1X Camera Tube for LAURE1 & LAURE2 Trinoculars, C-Mount, Male D2N Dovetail
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WFA4107 Support Documentation
WFA41071X Camera Tube for WFA4002 & WFA4003 Trinoculars, C-Mount, Male D5N Dovetail
$385.00
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WFA4108 Support Documentation
WFA41080.7X Camera Tube for WFA4002 & WFA4003 Trinoculars, C-Mount, Male D5N Dovetail
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WFA4109 Support Documentation
WFA41090.5X Camera Tube for WFA4002 & WFA4003 Trinoculars, C-Mount, Male D5N Dovetail
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Optional Upgrade: Stand-Alone Camera Tubes

Camera Tube Adjuster
Click to Enlarge

Fine Focus Adjuster (Item # SM1ZM) on Camera Tube
  • One WFA4100 is Included with the Cerna® Mini Microscope
  • Other Camera Tubes Offer Magnification
    • WFA4101: 0.75X
    • WFA4102: 0.5X
  • C-Mount Threads for Camera Attachment
  • 4.1 mm of Focus Adjustment for Camera
  • See the Full Web Presentation for More Information

These camera tubes provide the mechanical spacing necessary to align a camera sensor to the imaging plane in a microscope. The top of each camera tube has external C-mount (1.00"-32) threading that accepts Thorlabs' scientific cameras, as well as cameras from most major manufacturers. In order to balance the size of the field of view (FOV) displayed on the camera against the resolution of the microscope, our camera tubes are offered in several magnifications from 1X to 0.5X. Greater magnification improves the resolution, but it also decreases the size of the FOV. Please see the Magnification & FOV tab for more information.

The WFA4100, one of which is included with the Cerna mini microscope, includes a 200 mm tube lens to focus the image from the objective at the sample plane with 1X magnification. The WFA4101 and WFA4102 camera tubes use a 150 mm or 100 mm focal length tube lens, respectively, to produce 0.75 times or 0.5 times the magnification of the objective. To compensate for mechanical tolerances or any alignment issue, these camera tubes also include an SM1ZM Fine Focus Adjuster directly before the camera. This is shown in the photo to the right.

System Magnification
The total system magnification will be the multiplicative product of the objective magnification and the eyepieces or camera tube magnification, depending on which is being used. To achieve an objective's stated magnification, the objective must have been designed for systems using a 200 mm tube lens. Please note that the eyepieces and camera will see a different FOV due to a difference in magnification in each path. Please see the Magnification & FOV tab for more information on objective's magnification dependence on the system's tube lens and how paths with differing magnifications will see a different FOV.

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WFA4100 Support Documentation
WFA41001X Camera Tube with C-Mount, Male D1N Dovetail
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WFA4101 Support Documentation
WFA41010.75X Camera Tube with C-Mount, Male D1N Dovetail
$713.12
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WFA4102 Support Documentation
WFA41020.5X Camera Tube with C-Mount, Male D1N Dovetail
$438.25
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Optional Upgrade: Double Camera Ports for Scientific Cameras

2CM2 Mechanical Drawing
Click for Details

Mechanical Diagram of the 2CM2 Double Camera Port
2CM1 Mechanical Drawing
Click for Details

Mechanical Diagram of the 2CM1 Double Camera Port
Compatible Filters
Type Dimensions Thickness
Excitation Ø25 mm 5 mm
Emission Ø25 mm 3.5 mm
Dichroic Min 25.0 mm x 35.6 mm 1.0 mm
Max 25.2 mm x 36.0 mm 2.0 mm
Image Overlay from Two Cameras
Click to Enlarge

Live two-channel composite image generated using 2CM1 Mount, scientific CCD cameras, and ThorCam software. The image shows Fluorescence (Pink) and DIC (Grayscale) images of a mouse kidney.
  • Microscope Adapters Allow Two Scientific Cameras to Image a Single Optical Input Simultaneously
  • Optics Not Included:
    • Accepts 25 mm x 36 mm Dichroic Filters or Beamsplitters
    • Accepts Standard Ø25 mm or Ø1" Filters on Outputs
  • Fine Pitch Rotation and XY Adjustment for Image Co-Registration
  • Coarse Focus Adjustment for Parfocalization of Cameras
  • Can be Used with Inverted-Image Trinoculars or as Stand-Alone Double-Camera Ports

Thorlabs' two-camera mount are designed to attach two Thorlabs scientific cameras to a standard microscope, allowing simultaneous imaging of a single optical output. A rotation mount allows for 360° of rotational adjustment (±8° fine adjustment) for the reflected camera while a translation mount gives 4 mm linear XY adjustment of the transmitted camera. Both camera mounts have coarse focus adjustment by manually translating the cameras, allowing for parfocalization of both images. The 2CM1 and 2CM2 mounts have up to 15 mm and 11 mm of adjustment, respectively, using the cage rods, although this adjustment range may be limited by the geometry of the camera's front face.

Each mount includes our fluorescence filter cube, which is designed to hold a fluorescence filter set (dichroic mirror, excitation filter, and emission filter) as well as plate beamsplitters or other similarly sized optics. See the table to the right for compatible optic sizes. The filter cube has an insert to hold filter set components with a kinematic design for easy swapping between mounted filter sets without requiring realignment. A DFM1T1 filter cube insert for mounting additional filter sets is sold separately below. Please note that these mounts do not include tube lenses.

These camera ports are ideal for use with Thorlabs' scientific cameras and ThorCam software. The 2CM1 mount is designed for cameras with 60 mm cage system taps on the front, such as our scientific CCD cameras, while the 2CM2 mount is identical except for the inclusion of two LCP4S cage size adapters for compatibility with 30 mm cage system taps, such as those found on our compact scientific cameras with sCMOS and CMOS sensors. The ThorCam user interface, provided for free with our scientific cameras, includes a plug-in to allow for multiple live camera images to be overlaid into a real-time 2-channel composite, eliminating the need for frequent updates of a static overlay image. This live imaging method is ideal for applications such as calcium ratio imaging and electrophysiology.

To see example applications where these camera ports are used and how various filters and dichroics are used, please see the full presentation.

Cerna® Mini Microscope Compatibility

The input port of each camera port adapter has external SM1 (1.035"-40) threading and places Thorlabs scientific cameras' sensors at a distance of 4.05" to 4.17" (102.9 mm to 106.0 mm) from the base of the mount (see drawings to the upper right). An adapter can be mounted to a Cerna mini microscope with trinoculars or without trinoculars. These mounts are not compatible with the upright-image trinoculars.

Double Camera Port with Inverted-Image Trinoculars
These camera ports can be mounted directly onto our inverted-image trinoculars using the SM1A58 adapter (available below). Simply screw the adapter onto the base of the camera port and install it onto the top port of the trinoculars in place of the camera tube. When used with the inverted-image trinoculars, each mounted camera will be parfocal with the eyepieces. When used with upright-image trinocs the camera sensors will not be parfocal with the eyepieces.

Stand-Alone Double Camera Port
The double camera port can be installed without trinoculars by connecting the WFA4110 Dovetail Adapter with 200 mm Focal Length Tube Lens to the camera port using two lens tubes (SM2L10 and SM1M05) and an SM1A2 adapter. To mount this assembly onto the Cerna mini microscope, loosen the M4 setscrew at the top of the epi-illuminator module, remove the included camera tube, and replace it with the double camera port assembly. Tighten the M4 screw to lock the dovetail at the base of the WFA4110 adapter in place.

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2CM1 Support Documentation
2CM1NEW!Two-Camera Mount for Microscopes, 60 mm Cage Mount Compatible
$1,800.00
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2CM2NEW!Two-Camera Mount for Microscopes, 30 mm Cage Mount Compatible
$1,880.00
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DIY Accessories: Dovetail Adapters

  • LCPN2 Trinocular Port Adapter
    • Male D1N Dovetail
    • Internal SM30 Threads (M30.5 x 0.5)
    • 30 mm and 60 mm Cage Compatible
  • LCPN3 Trinocular Port Adapter
    • Compatible with Olympus Trinoculars
    • Male D1N Dovetail and Female D5Y Dovetail
    • Internal SM30 Threads (M30.5 x 0.5)
    • 60 mm Cage Compatible
  • WFA4111 Adapter
    • Male D1N Dovetail Mates to Top of Cerna® Mini Epi-Illuminator Module
    • External SM2 (2.035"-40) Threads for our SM2 Lens Tubes
    • Internal M38 x 0.5 Threads Accept TTL200 Tube Lens
  • WFA4110 Adapter
    • All WFA4111 Mounting Features
    • Includes 200 mm Focal Length Tube Lens
  • SM1A58 Camera Port Adapter
    • Male D2N and D2NB Dovetails
    • Internal SM1 (1.035"-40) and External SM2 Threads
    • 30 mm Cage Compatible

Our Dovetail Adapters allow users to easily integrate custom assemblies that use our extensive line of optomechanical components with the Cerna mini microscope.

WFA4111 and WFA4110 Adapters

The WFA4111 and WFA4110 adapters feature a male D1N dovetail on the bottom that allows the adapter to mate directly to the top of the Cerna mini epi-fluorescence microscope's filter cube housing. External SM2 threads at the top of the module enable the construction of custom light-tight optical paths using our SM2 lens tube systems. The WFA4110 includes a 200 mm focal length tube lens to support custom camera ports, while the WFA4111 has an M38 x 0.5 threaded central bore to accept user-selected optics. The SM38RR retaining ring can be used to lock the optics in place when using the WFA4111 adapter.

SM1A58 Camera Port Adapter
The SM1A58 Adapter has a male D2N dovetail and internal SM1 threads that allow a double camera port to be added to inverted-image trinoculars (available above). External SM2 threads and taps for our 30 mm cage system also support the construction of custom modules.

LCPN2 and LCPN3 Trinocular Port Adapters
The LCPN2 and LCPN3 adapters have a male D1N dovetail and feature internal SM30 (M30.5 x 0.5) threading for Ø30 mm lens tubes; two SM30RR retaining rings are included to secure an optic inside the adapter. Four through holes, each with two side-located locking setscrews (5/64" [2 mm] hex), can be used to attach Ø6 mm cage rods for 60 mm cage systems. The LCPN2 adapter is designed to be used with custom widefield viewing setups and includes additional 4-40 tapped holes on 30 mm centers on the side opposite the dovetail for 30 mm cage systems. The LCPN3 adapter features a female D5Y dovetail to allow Olympus trinoculars with a male D5Y dovetail to be used with the Cerna mini microscope. 

Item # WFA4111 WFA4110 SM1A58 LCPN2 LCPN3
Dovetail Male D1N Male D2N Male D1N Male D1N
Female D5Y
Threading Internal M38 x 0.5a
External SM2 (2.035"- 40)
External SM2 (2.035"- 40) Internal SM1b (1.035"- 40)
External SM2 (2.035"- 40)
Internal SM30 (M30.5 x 0.5) Internal SM30 (M30.5 x 0.5)
Cage Compatibility Use an SM2-Threaded Cage Plate to Add
60 mm Cage Compatibility
30 mm Cage System
(4-40 Tap, 4 Places)
30 mm Cage System
(4-40 Tap, 4 Places)
60 mm Cage System
(Ø6 mm Bore, 4 Places) 
60 mm Cage System
(Ø6 mm Bore, 4 Places)
Clear Aperture Ø1.47" (37.0 mm) - Ø1.008" (25.6 mm) Ø1.10"  (27.9 mm) Ø1.10" (27.9 mm)
Built-in Tube Lens - TTL200 - - -
Adapter Profile
(Click for Drawing)
  • This internal M38 x 0.5 threading is compatible with our SM38RR retaining rings.
  • This internal SM1 threading is not deep enough for mounting optics.
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WFA4111Adapter with Male D1N Dovetail, External SM2 Threads, and Internal M38 x 0.5 Threads
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WFA4110Adapter with Male D1N Dovetail, External SM2 Threads, and TTL200 Tube Lens
$618.97
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SM1A58 Support Documentation
SM1A58Upright Nikon Eclipse and Thorlabs Cerna Microscope Camera Port Adapter, Internal SM1 Threads, External SM2 Threads, 30 mm Cage Compatible
$81.70
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LCPN2 Support Documentation
LCPN2Nikon Eclipse or Cerna Microscope Trinocular Adapter, Male D1N Dovetail, Internal SM30 Threads, 30 and 60 mm Cage Compatibility
$111.39
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LCPN3 Support Documentation
LCPN3Customer Inspired! Nikon Eclipse or Cerna Microscope Trinocular Adapter, Male D1N Dovetail, Female D5Y Dovetail, Internal SM30 Threads, 60 mm Cage Compatibility
$73.34
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DIY Accessories: Housing for Custom Epi-Illuminator Module


Click to Enlarge

The front of the module has a magnetic door cover that holds a filter cube.

Click to Enlarge

The back of the WFA2002 module has internal SM1 threads and four 4-40 taps for our 30 mm cage system.
  • Internal SM1 (1.035"-40) Threads, Four 4-40 Taps for 30 mm
    Cage Systems, and Female and Male D1N Dovetails
  • Magnetic Door Cover Holds Filter Cube in Optical Path
This animation shows the installation of a loaded filter cube into the WFA2002 Epi-Illuminator Module.

The WFA2002 Epi-Illuminator Module holds one filter cube and is the base module for the epi-illumination pathway of a Cerna® mini epi-fluorescence microscope. Additional modules are offered separately to support the addition of customer-built epi-illuminator modules. With a female D1N dovetail on top and a male D1N dovetail on the bottom, the WFA2002 is designed to mate with the epi-illumination arm of the microscope body, as well as with other epi-illuminator modules.

As shown in the image to the right, its optical input port has internal SM1 (1.035"-40) threads and four 4-40 taps for our 30 mm cage system. These mechanical interfaces enable Thorlabs' extensive selection of SM1 and 30 mm cage components to be used to build custom epi-illumination paths.

The module includes a magnetically secured cover that can be connected to a MDFM-MF2 filter cube, manufactured by Olympus (available above). Thorlabs' filter cubes provide reduced optic distortion and simpler optic mounting. The animation to the left shows the filter cube installation procedure. Extra covers, which the user can attach to the filter cubes to speed up filter cube exchange, are sold as Item # WFA2001C (available above). These filter cubes can be used to hold fluorescence filter sets, beamsplitters with crossed polarizers, or mirrors.

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WFA2002 Support Documentation
WFA2002Epi-Illuminator Module for 1 Cube, Male & Female D1N Dovetails
$419.06
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