Features
- Excitation, Emission, and Dichroic Filters for Fluorescence Imaging
- Provide >90% Transmission at the desired Wavelength
- Sharp Cutoff (T < 0.001%) Outside the transmission Band
- For use with Green Fluorescent Protein (GFP)
- Excitation and Emission Filters are Mounted in Black Anodized Housings
- Dichroic Filters are Unmounted
These excitation, emission, and dichroic filters are designed specifically for use in fluorescence imaging applications. The Ø25 mm excitation and emission filters are mounted in black anodized housings that are 5 mm and 3.5 mm thick, respectively, while the 25.2 mm x 35.6 mm dichroic filters are unmounted.
Filter Design
These Filters are manufactured to high-performance optical specifications and designed durability. They are produced with multiple dielectric layers deposited on a high-precision, fused silica substrate. The substrate is ground and polished to ensure that the highest possible image quality is maintained. These Hard-coated optics produce filter layers that are denser than those obtained from electron beam deposition techniques. The dense filter layers reduce water absorption and greatly enhance durability, stability, and performance of the filter. Each layer is monitored during growth to ensure minimal deviation from design specification thickness, ensuring overall high-quality filter performance. All filters conform to MIL-STD-810F and MIL-C-48497A environmental standards.
Specifications
- Material: Fused Silica
- Size: Ø25 mm (Excitation/Emission); 25.2 mm x 35.6 mm (Dichroic)
- Thickness: 5.0 ± 0.1 mm (Excitation); 3.5 ± 0.1 mm (Emission); 1.05 ± 0.05 mm (Dichroic)
- Dimensional Tolerance: ±0.1 mm
- Surface Quality: 60-40 Scratch-Dig
- Angle of Incidence: 0° ± 5° (Excitation/Emission); 45° ± 1.5° (Dichroic)
- Fluorophore: Green Fluorescent Protein (GFP)
- Excitation: 469 ± 17.5 nm
- Emission: 525 ± 19.5 nm
- Dichroic (Reflection/Transmission): 452 - 490 nm / 505 - 800 nm
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