Adaptive Scanning Optical Microscopy (ASOM) has been shown to be useful in high throughput pathology screening, as demonstrated by researchers at RPI with an adaptive scanning optical microscope prototype. The fast steering mirror (FSM) allows rapid data collection over a large field of view, while the deformable mirror (DM) maintains high resolution (1.5 µm) as shown in the images of the breast tissue pathology slide below.
Figure 1: Breast tissue slides were stained for the growth hormone receptor system, which indicates cancerous cells if it is over expressing proteins. The ability to create images with cellular level resolution, while getting gross anatomical morphology, can greatly aid the detection and identification of cancerous tissue. Tile scanning using the FSM makes data acquisition rapid, while clearly identifying abnormal cell locations in a gross pathology sample.
Figure 2. The images shown here illustrate the tile-scan mosaic data collection, demonstrated with a sample of human skin and sweat gland mounted on a standard microscope slide. On the left: the three images from top to bottom are screen shots of the mosaic imaging at 1.3 seconds, 6.4 seconds, and 12 seconds, respectively. As the design of ASOM matures, the acquisition speed is expected to double every 3-6 months.
The DM and FSM design incorporated in the new Thorlabs’ commercial ASM900 system makes pathology screening quicker, easier, and more accurate. By providing cellular-level resolution and a large field of view, the full plane of the pathology slice can be rapidly imaged, eliminating the need for multiple scans with a moving stage, enabling high throughput slide processing. The high resolution obtained in a single scan provides facile determination of cell abnormalities for accurate clinical diagnoses.
Image Courtesy: Benjamin Potsaid, John T. Wen, and Yves Bellouard, Smart Optics Lab, Center for Automation Technologies, Rensselaer Polytechnic Institute, New York.
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