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Welcome to Thorlabs ASOM Gallery
Anatomy and Developmental Biology
Adaptive Scanning Optical Microscopy (ASOM) is useful in anatomy and developmental biology studies due to the high resolution and large viewing field (up to 1250 mm2). Traditional microscopy is limited due to the fact that the field of view becomes smaller as the magnification increases. This means that obtaining high resolution images is limited to a select region of the sample, or that the sample must be moved and multiple data collection scans obtained. In contrast, the fast steering mirror (FSM) and deformable mirror (DM) used in ASOM retains cellular-level resolution (1.5 µm) while simultaneously allowing the entire sample to be observed. This is demonstrated below using Thorlabs’ ASM9600 in transmission mode. Figure 1a-c shows a sample of rabbit lymph node, with high resolution imaging of specific regions of interest that were obtained directly during the scan without changing objectives, as would be required in traditional microscopy. Figure 2 demonstrates the usefulness of this technology in developmental biology studies. In this case, the complete organism can be imaged at high resolution in order to observe development and differentiation, demonstrated here with a mosquito larva.
Figure 1: These three images show a slide of rabbit lymph node mounted on a standard microscope slide. The images were taken using the ASM9600 in transmission mode. The first image (Figure1a) shows the entire structure, while the two other images (Figures 1b and 1c) demonstrate cellular-level resolution capabilities of the ASOM, by zooming in on the sinus and a cross-section of a lymphatic vessel.
Figure 2: Image of mosquito larva taken using the ASM9600 in transmission mode. The total digital image measures 8,192 x 6,144 pixels and was created using a 8 x 8 tile mosaic.
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