Thorlabs' Dispersion-Compensating Mirror Set corrects for the pulse broadening that occurs when ultrashort pulses propagate through an optical system (e.g., a laser scanning microscope setup).
Since a femtosecond laser pulse consists of many different wavelengths, pulse broadening (a lengthening of the temporal intensity profile) will occur when the pulse passes through a dielectric medium, like glass. This broadening is caused by the wavelength dependence of the refractive index of the optical components through which the light travels. In typical glass, shorter wavelengths have higher indices of refraction than longer wavelengths, causing shorter wavelengths to travel slower. These mirrors are specifically designed so that longer wavelengths experience larger group velocity delay than shorter wavelengths, allowing the shorter wavelengths to "catch up" to the longer wavelengths.
Pulse broadening decreases the peak power of the femtosecond laser pulse. Since the intensity of the generated fluorescence depends on the intensity of the excitation pulse, correcting for pulse broadening enhances the image contrast. A demonstration is available in the Application tab.
The highly reflective coating for the 700 - 1000 nm range is deposited on the surface using ion beam sputtering (IBS). This highly repeatable and controllable technique results in durable thin film coatings with high damage thresholds.
As shown in the figure to the right, these mirrors can be mounted in the Kinematic Grating Mount Adapter, which is compatible with Ø1", front-loading, unthreaded mirror mounts, such as our Polaris Ultrastable Kinematic Mirror Mount.
|Wavelength Range||700 - 1000 nm|
|Reflectivity Over Wavelength Range||>99%|
|Group Delay Dispersion (GDD)|
|-175 fs2 at 800 nm|
|Clear Aperture||At Least 10 mm x 50 mm|
|Surface Flatness (Peak to Valley)||λ/10 Over Any Ø10 mm in the Clear Aperture|
|Surface Quality||10-5 Scratch-Dig|
|Damage Threshold||0.1 J/cm2|
(100 fs Pulses Centered at 800 nm)
|Substrate Material||Fused Silica|
|Dimensions (L X W X D)||52.0 mm x 11.0 mm x 12.0 mm|
(2.05" x 0.43" x 0.47")
Multiphoton microscopy is recognized as the premier method for obtaining high-resolution, three-dimensional images from within thick biological samples. Compared to confocal microscopy, multiphoton imaging features more efficient fluorescence detection and reduced photodamage. Furthermore, the use of near-infrared excitation allows for improved imaging depth due to a reduction in scattering losses at the surface.
Since multiphoton microscopy requires simultaneous absorption of two or more photons by the fluorophore, mode-locked pulsed lasers with high repetition rates and ultrashort pulses are employed. These lasers provide the high peak powers necessary to generate sufficient multiphoton absorption in the focal plane while preventing damage to the sample by keeping the average power absorbed low.
However, the standard optical components found in most imaging systems tend to broaden ultrashort laser pulses to the point where the quality of the image will be affected. This pulse broadening occurs because of the wavelength dependence of the refractive index of optical components through which the light travels; shorter wavelengths have higher indices of refraction in glass than longer wavelengths, causing shorter wavelengths to travel slower.
The dispersion of the laser pulse is corrected using dielectric mirrors with specialized coatings that are specifically designed so that longer wavelengths experience a larger group delay than shorter wavelengths, thereby negating pulse broadening caused by other optical elements within the imaging system. The two-photon images of a mouse kidney shown below demonstrate the benefits of using the Dispersion-Compensating Mirror Set for increasing image quality.
|Figure 1. Pseudocolored multiphoton images of a mouse kidney obtained without (frame a) and with (frame b) the use of a Dispersion-Compensating Mirror Set. The glomeruli and convoluted tubules are stained with Alexa Fluor 488 (green) while the nuclei are stained with DAPI (blue). By introducing the mirror pair into the setup, the signal to noise was increased by a factor of approximately 38, thereby providing a higher quality image of the mouse kidney.|
The figure to the right shows an images of a mouse kidney specimen that were taken prior to and after inserting the Dispersion-Compensating Mirror Set into the setup. In the specimen (Molecular Probes®, Invitrogen Corp.), the glomeruli and convoluted tubules were labeled with Alexa Fluor 488 (green), while the cell nuclei were labeled with DAPI (blue).
Simultaneous excitation of both fluorophores was accomplished using a Ti:Sapphire oscillator that produces ultrashort (<6 fs) pulses with a 1 GHz repetition rate. Two-photon fluorescence images were obtained with Thorlabs' in-house multiphoton microscope equipped with a 40X objective (Olympus, NA = 0.50).
As shown in the figure, the introduction of a dispersion-compensating mirror set into the experimental setup prior to the imaging optics dramatically improves the quality of the image. Figure 1a shows an image of a mouse kidney specimen that was taken without the use of the mirror set. The group delay dispersion (GDD) attributed to the optical elements in the multiphoton microscope is ~4200 fs2 at 800 nm. Figure 1b shows the same image acquired by using the dispersion-compensating mirror set to negate the GDD of the imaging optics. An intensity analysis of Fig. 1 indicates that the signal-to-noise ratio increased by a factor of approximately 38 (16 dB).
Dispersion Compensating Mirror Set (2 Pieces)