Fast Switching and Intensity Adjustments via LED Current Settings
High Emission Stability and Reproducibility
High Thermal and Mechanical Stability
Rugged and Vibration Free: No Moving Parts
Long Lifetime Light Source
54 Combinations of 10 LED Wavelengths (see table on the right)
Optional Adapters for Olympus, Nikon, Zeiss and Leica Microscope Lightports
The new LED4C Series 4-Wavelength LED Sources are ideal for application requiring up to four wavelengths of high-performance light. These light sources efficiently combine all of the beams into a single collimated emission. Together with the DC4100 4-Channel Driver, the LED4C forms a reliable, versatile light source that offers fast switching and accurate modulation of each individual LED. The source is vibration free, easy to operate, and offers a long lifetime (see the Specs tab for details). The LED4C Series source is available with 54 different preselected combinations of 10 LED wavelengths. Any combination of four wavelengths can be combined except for the pairings listed below. The output aperture of these LED sources is SM2 (2.035"-40) internally threaded. For Olympus, Nikon, Zeiss, and Leica microscopes, optional adapters are available that directly mount the 4-Wavelength Sources onto the microscope lightport (see SM2-Threaded Microscope Adapter).
The LED4C contains four individual bare LEDs. These LEDs have high divergence and the light is guided through the system using mirrors. The output is collimated with an aspheric condenser lens. This setup inherently leads to a reduction in the measured output power from that of the bare LEDs. Thorlabs also offers single-channel collimated LEDs, which have higher power outputs due to the lack of the wavelength combining optics.
The following LED wavelength combinations cannot be included in the same LED4C source:
385 nm and 405 nm
455 nm and 470 nm
455 nm and 505 nm
505 nm and 530 nm
617 nm and 625 nm
625 nm and 660 nm
Compared to conventional, non-LED sources, these 4-wavelength sources offer many advantages such as higher signal-to-noise ratios due to narrow bandwidth emission, easy system integration, simple operation without maintenance cycles, and no active cooling requirements or thermal filtering due to minimal thermal loads. The operation wavelength and intensity can be set quickly, making them an ideal choice for microscopy applications where multiple wavelengths and short time constants are required.
This is a response from Thomas at Thorlabs. Thank you very much for your inquiry. Unfortunately we do not have this information but I will be very happy to simulate it for you. I will contact you with more detailed information. Best Regards, Thomas
Poster: mhawks
Posted Date: 2012-12-19 13:29:54.733
Do you have any specifications on how well-collimated the beam is?
Poster: tschalk
Posted Date: 2012-12-19 10:26:00.0
This is a response from Thomas at Thorlabs. Thank you very much for your inquiry. It is possible to integrate an excitation filter into the 4 Wavelength High-Power LED Source. This filter will influence all four wavelengths. I will contact you directly for more detailed information. Best Regards, Thomas
Poster: tschalk
Posted Date: 2012-12-19 09:50:00.0
This is a response from Thomas at Thorlabs. Thank you very much for your inquiry. Unfortunately we do not have a setup yet were it is possible to couple light from a 4-color LED source into a liquid light guide. However, you could use a lens tube, for example SM2L30, with an aspheric condenser lens, for example ACL5040-A. The lens tube fits into the SM2 threading on the 4-color LED source. To mount a liquid light guide you can use two adapters, SM2A6 and AD5LLG. It is a good idea to have a complete setup with an aligned lens. I will forward this idea to our development department so that we will be able to offer a part like this in the future. Best Regards, Thomas
Poster: heyderc
Posted Date: 2012-12-18 17:31:48.753
I would like to know if the 4-color LED source can be coupled to a liquid light guide. Thanks, Chris
Poster: heyderc
Posted Date: 2012-12-18 15:20:11.607
I would also like to know if it is possible to fit excitation filters into the LED source. Thanks.
Poster: tschalk
Posted Date: 2012-11-14 06:29:00.0
A response from Thomas at Thorlabs: Thank you for your inquiry! The lower power is caused through losses inside the housing. The divergence of the LED-Chips is very high and the beam is guided with mirrors to the condenser lens for collimation. Because of this setup a lot of optical energy gets lost. The lens of a single collimated LED is located directly in front of the LED-Chip and this causes almost no losses.
Poster: eozkan
Posted Date: 2012-11-12 21:47:25.213
Hello,
Could you help me understand why power measurements on 4 wavelength high power led source are much lower single wavelength collimated led sources?(compare 470 for example: 25 mW on this product vs. >800 if purchased seperately)
thanks,
Poster: jvigroux
Posted Date: 2012-06-20 10:04:00.0
A response from Julien at Thorlabs: Thank you for your inquiry! This should be in principle possible but will largely depend on the exact combination of wavelengths you need. I will contact you directly to see which possible combination would work best for you
Poster: dario.protti
Posted Date: 2012-06-20 01:11:26.0
would it be possible to use 3 LEDs in the visible range and one infrared (~850nm)? many thanks in advance
Poster: jvigroux
Posted Date: 2012-01-26 07:37:00.0
A response from Julien at Thorlabs: Thank you for your inquiry! The LEDs will yield about the same spectral irradiance as a HOB mercury lamp. The LED however will have wider spectral distribution than the individual mercury lamps such that their efficiency will also depend on the width of the fluorescent proteins you use. I will contact you directly to further discuss those points
Poster: ron.goldstein
Posted Date: 2012-01-24 11:44:04.0
I would like to use this light source for live imaging of cells with fluorescent proteins GFP/mCherry etc.
I currently use a 100W HBO mercury lamp and exciter filters. How does the INTENSITY of the light from this LED source compare to that of at mercury lamp at 455/590/627?
Thanks
Poster: jvigroux
Posted Date: 2011-12-19 13:09:00.0
a response form Julien at Thorlabs: Thank you for your feedback! the design of the LED4C LED source only allows the simultaneous use of four different wavelengths. Further, there is to our knowledge no high power LED available at 340nm. We should discuss more closely what the exact requirements for your application are as based on the exact output power required, there might be some other type of setup allowing to achieve the wavelength combination you need. Unfortunately, for now the use of the software within MetaMorph/MetaFluor is not yet implemented. The suggestion to emulate the Sutter filter wheel seems like a goop approach. Our software engineers are having a look into this and I should be able to post further information about the feasibility of such approach within the next 2-3 weeks.
Poster: gmcnamara
Posted Date: 2011-12-16 19:03:14.0
I would like to use a 4LED - or better yet 5LED - for Fura-2 Calcium ion ratio imaging, plus GFP, DsRed and Cy5/Alexa Fluor 647 (not necessarily at one time). I will need:
~340 nm LED (in addition to 385, 470, 530 and hopefully 627 LEDs).
specific bandpass filtes to eliminate the tails of the LED spectra - especially so that I can have a clean separation of ~340 nm from ~385 nm light.
I can select the appropriate filter sets for inside my microscope, once you/I/we figure out the LED bandpass filters. Probably a Fura-2 + Cy5 filter set and a DAPI/Green/Red triple pass set.
I find your 4LED + microscope adapter product selection line-up on your web site confusing.
I need more useful software integration than uManager. You never posted a follow-up to the 2009-11-24 posting about third party software. I am especially interested in MetaMorph/MetaFluor. If you do not want to work with vendors one by one, a suggstion: consider emulating a Sutter 10-2 filter wheel and shutter, with USB control (you can use the uManager Sutter interface to test the emulation).
Poster: bdada
Posted Date: 2011-11-08 10:53:00.0
Response from Buki at Thorlabs:
Thank you for your interest in our 4 wavelength LED source. We will contact you for more information about your applications and the options for customization of the unit.
Poster: richard.norman
Posted Date: 2011-11-07 18:25:14.0
Interested in using it for a different application, but I'm having difficulty determining the output levels. May need different output optics. Can they be changed? Contact me and I'll explain the application.
Poster: jvigroux
Posted Date: 2011-08-10 11:48:00.0
A response from Julien at Thorlabs: Thank you for your feedback. As of now, we do not have dichroic mirrors that allow to cover the necessary spectrum for the combination you need (from 450nm to 850nm). This is the main reason why the maximum wavelengths for those four colors LEDs is about 670nm. Maybe an different arrangement using single LEDs could also work for your application. I will contact you directly to discuss this approach.
Poster: pbenzel
Posted Date: 2011-08-09 11:53:30.0
Can a LED4C1 be ordered with the following wavelengths 455, 530, 627, 850nm?
Poster: bdada
Posted Date: 2011-04-26 12:14:00.0
Response from Buki at Thorlabs:
Thank you for your feedback. The DC4100 controller allows you to switch on all 4 LEDs at once. Please refer to page 11 of the DC4100 manual linked below to learn more about the multi selection mode:
http://www.thorlabs.com/Thorcat/18300/18321-D02.pdf
You can also access the manual and other technical documents for each product on our website. If you have any questions, please contact TechSupport@thorlabs.com.
Poster: michael.halter
Posted Date: 2011-04-26 10:25:40.0
Can the light from all four LEDs be output at the same time (such that I could filter all light in the microscope body)?
Poster: karl.farrow
Posted Date: 2011-03-15 11:14:36.0
Im very interested in purchasing this product but with a different set of mounted LEDs.
Could you please contact me to discuss if this is possible.
Thanks
Karl Farrow
Poster: jjurado
Posted Date: 2011-03-15 08:46:00.0
Response from Javier at Thorlabs to Karl Farrow: I will contact you directly to discuss your application.
Poster: julien
Posted Date: 2010-12-08 05:22:39.0
A response from Julien at Thorlabs: Dear Shin-young, the LED4CX have an internal SM2 thread on which the microscope adapters LED4AX can be directly mounted. I will contact you directly to see exactly what the problem with your adapter is.
Poster: ryusy123
Posted Date: 2010-12-07 07:06:28.0
I purchased LED4C and olympus adapter but I cannot find the right way to mount this LED4C with the olympus adapter LED4A1.
my microscope is olympus IX71.
Please let me know how to mount
Thank you.
Shin-Young Ryu
Poster: egentzsch
Posted Date: 2009-11-24 16:42:30.0
A response from Erik at Thorlabs to lhall: This driver has its own software (Graphical User Interface) and software drivers. Currently it supports only the software µManager. I will check if it is possible to integrate it into third party software.
Poster: lhall
Posted Date: 2009-11-20 08:48:06.0
Hi again,
Forgot to ask if this system will work with Metamorph, Slidebook or Olympus SIS software or other software.
Please advise.
Lance R. Hall
Leeds
Poster: lhall
Posted Date: 2009-11-20 08:44:57.0
Has this been used for fluorescent application, most noteably for GFP and CFP.
thanks,
Lance R. Hall
Senior Technical Sales
Leeds Instruments, Inc.
San Antonio, Texas
Cell: (210) 421 4953
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4-Channels with Precise and Individual Current Adjustment
Modulation Frequency: 0 - 100 kHz
LED Current: 1 A (Max)
Driving Voltage: 5 V (Max)
External Modulation: 0 - 10 V
Precise Intensity Adjustment
The DC4100 Series LED Driver is designed to conveniently control the LED4C Series Source. For more details please see the Product Page of the DC4100 Series