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Imaging/Focusing Microscope Objectives


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Imaging/Focusing Microscope Objectives

Thorlabs offers both air and oil-immersion designs of Olympus and Nikon visible imaging objectives. Plan achromat and plan fluorite (also called plan semi-apochromat or plan fluor) objectives are available. For more details about the differences between these types of objectives, please see the Objective Tutorial tab above.

The infinity-corrected objectives have elements with an ultra-wide broadband AR coating. They are compatible with many of the microscopy and fiber coupling accessories offered by Thorlabs. All of the Olympus objectives on this page have RMS (0.800"-36) threading, while the Nikon objectives have M25 x 0.75 threading.

These microscope objectives should be used in applications like traditional microscopy, confocal imaging, and fiber coupling. When choosing a microscope objective, it is important to keep in mind that objectives are often designed to integrate with a particular manufacturer's microscopes and are not necessarily interchangeable due to tube length differences and variations in thread pitch or diameter.

Our microscope objectives can be mounted directly to our fiber launch systems, or they can be mounted to any of our flexure or translation stages using an HCS013 RMS mount. Alternatively, these microscope objectives can be easily mounted to our cage systems (using an SM1A3 adapter) or to our lens tubes, thus facilitating the setup of optical systems.

Olympus Plan Achromat Objectives

Item #RMS4XRMS10XRMS20XRMS40X
Diameter*24 mm24 mm24 mm24 mm
Length*30.89 mm38.79 mm48.49 mm48.79 mm
Magnification4X10X20X40X
Numerical Aperture (NA)0.100.250.400.65
Field Number22222222
Working Distance (WD)18.5 mm10.6 mm1.2 mm0.6 mm
Effective Focal Length (EFL)45 mm18 mm9.0 mm4.5 mm
Parfocal Length*45.06 mm
Wavelength RangeVisible
ThreadingRMS**

*These dimensions are defined in the drawing below.
**An SM1A3 adapter allows an RMS objective to be used with our SM1 lens tubes.

Olympus Plan Fluorite Objectives

Item #RMS4X-PFRMS10X-PFRMS20X-PFRMS40X-PFRMS60X-PFC
Diameter*24 mm24 mm26 mm26 mm28 mm
Length*32.39 mm39.39 mm47.29 mm48.88 mm49.39 mm
Magnification4X10X20X40X60X
Numerical Aperture (NA)0.130.30.500.750.9
Working Distance (WD)17 mm10 mm2.1 mm0.51 mm0.2 mm
Parfocal Length*45.06 mm
Wavelength RangeVisible and NIR
ThreadingRMS**

*These dimensions are defined in the drawing below.
** An SM1A3 adapter allows an RMS objective to be used with our SM1 lens tubes.

Nikon Plan Fluorite Objectives

Item #N4X-PFN10X-PFN20X-PFN40X-PFN60X-PF
Magnification4X10X20X40X60X
Numerical Aperture (NA)0.130.30.500.750.85
Working Distance (WD)17.2 mm16 mm2.1 mm0.66 mm0.31 - 0.4 mm
ThreadingM25 x 0.75*

*An SM1A12 adapter allows an M25 x 0.75 objective to be used with our SM1 lens tubes.

Olympus Oil-Immersion Objectives

Item #RMS40X-PFORMS60X-PFODRMS100X-PFOaRMS100X-PFODRMS100X-O
Diameterb28 mm28 mm26 mm26 mm24 mm
Lengthb49.39 mm49.47 mm49.19 mm49.19 mm49.24 mm
Magnification40X60X100X100X100X
Numerical Aperture (NA)1.31.25 - 0.651.31.3 - 0.61.25
Working Distance (WD)0.2 mm0.12 mm 0.2 mm0.2 mm0.15 mm
Parfocal Lengthb45.06 mm
Design WavelengthVisible and NIRVisible
Threading RMSc

a. The high NA of this objective also makes it suitable for use with our Optical Tweezer kit.
b. These dimesions are defined in the drawing below.
c. An SM1A3 adapter allows an RMS objective to be used with our SM1 lens tubes.

Nikon Oil-Immersion Objective

Item #N100X-PFO*
Magnification100X
Numerical Aperture (NA)1.3
Working Distance (WD)0.2 mm
ThreadingM25.5 x 0.75*

*The high NA of this objective also makes it suitable for use with our Optical Tweezer kit.
**An SM1A12 adapter allows an RMS objective to be used with our SM1 lens tubes.

Dimensional Drawing

Olympus Objective Dimensional Drawing

Types of Objectives

Thorlabs offers several types of objectives from Nikon and Olympus. This guide describes the features and benefits of each type of objective.

Air or Oil-Immersion Objectives
This designation refers to the medium that should be present between the front of the objective and the cover glass of the microscope slide. Air objectives are designed to work best with an air gap between the objective and the specimen, while oil-immersion objectives require the use of a drop of immersion oil (such as MOIL-30) between and in contact with the front lens of the objective and with the cover glass. Oil immersion is required in order to achieve numerical apertures greater than 1. Note that if an oil immersion objective is used without the oil present, the image quality will be very low.

Plan Achromat Objectives
"Plan" designates that these objectives produce a flat image across the field of view. "Achromat" refers to the correction for chromatic aberration featured in the lens design. These objectives have chromatic correction for three wavelengths, and feature spherical correction at two wavelengths. Plan achromats produce their best images for green light. In white light, the plan achromats give satisfactory images for color photomicrography, but the results are not as good as objectives that feature better correction, such as plan fluorite objectives (see below).

Plan Fluorite Objectives
Plan fluorite objectives, also referred to as plan semi-apochromats or plan fluours, also produce a flat image across the field of view. Plan fluorite objectives are corrected for chromatic and spherical aberrations at four wavelengths. Plan fluorite objectives work well for color photomicrography.

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Posted Comments:
Poster: tcohen
Posted Date: 2012-06-21 15:54:00.0
Response from Tim at Thorlabs: Thank you for your interest in our objectives! We currently do not have operating temperature specs for these objectives. I will contact you to find out which objective you are interested in data for so that we may provide more information.
Poster: elizabethdoesphysics
Posted Date: 2012-06-19 13:34:42.0
Are there specs stating the operable temperature range for these objectives? We are considering heating a sample and viewing it with an objective. However, the objective may get hot during the process (it takes about 1.5 days at a temperature of about 50 to 60 degrees C). Would these microscope objectives tolerate such use?
Poster: bdada
Posted Date: 2012-01-23 09:56:00.0
Response from Buki at Thorlabs: Thank you for using our feedback forum. An objective with 10, 20, 40X, etc and no Focal Length designation will typically have an EFL equal to the "Tube Length" divided by the magnification. Typical tube length is 200mm so for a 10X objective the EFL is 200/10 = 20mm EFL. This is the approximate EFL and NOT the working distance. Working distance for a 10X objective would be somewhere around 15mm. There are other common tube lengths but the more common ones are 200mm and 160mm. Please contact TechSupport@thorlabs.com if you have any questions.
Poster: david.szwer
Posted Date: 2012-01-20 13:09:43.0
Dear ThorLabs, I am considering buying one of the infinity-corrected microscope objectives for use as an output collimator for an optical fibre. The output beam radius is very important, and I can calculate it using the fibre's numerical aperture, and the objective's effective focal length. However, I can't find details of the effective focal lengths for these objectives. Is there another way to calculate the beam size using the information you give? Best regards, David.
Poster: jjurado
Posted Date: 2011-03-18 10:35:00.0
Response from Javier at Thorlabs to Lehmann: Thank you for contacting us with your request. The entrance aperture for these microscope objectives can be calculated using the following formula: EA = 2 * EFL * tan [arcsin(NA)]. So, for an objective like the RMS4X, the entrance aperture is 9.1 mm. I will contact you directly for further support.
Poster: Lehmann
Posted Date: 2011-03-17 13:05:06.0
I have a question about these microscope objectives. I need to focus a laser to a certain spot size. The given values for the NA is based, I assume, that one fills the input lens. In order to calculate the effective NA for an input TEM00 beam, I must know the input size corresponding to the limiting aperture, but I do not see this listed. Perhaps this the effective focal length times the listed NA. Please pass along information that will allow me to calculate the focal spot size from the wavelength and input beam diameter, assuming a TEM00 beam.
Poster: Thorlabs
Posted Date: 2010-07-27 17:55:26.0
Response from Javier at Thorlabs to kearly500: Thank you for your feedback. We do not have concise values for the damage threshold of these lenses. However, as a guideline, Olympus recommends limiting the power input to <20mW for focused light and <100mW for collimated light.
Poster: kearly500
Posted Date: 2010-07-27 11:40:40.0
Hi, Im wondering what the damage threshold is for the RMS40X. Were using it to fiber couple a 2W solid state laser at 532 nm, and I wanted to know if we need to attenuate beforehand.
Poster: Adam
Posted Date: 2010-04-15 09:32:19.0
A response from Adam at Thorlabs to Albert: While it is possible to use the RMS threaded objectives with the DCC1645C, I think we need more information about your application before we confirm. We would need to know information about your object size and distance. I will email you directly to get this information. On a side note, to connect the RMS threaded objective to the DCC1645C, one would need to use a SM1A3(SM1 to RMS threaded adapater), and a SM1A9(SM1 to C-mount threaded adapter).
Poster: albert
Posted Date: 2010-04-15 06:14:07.0
Dear Sirs, We are going to develop a digital microscopic imaging system by using your DCC1645C, MAX203, BSC102 and objective lens(RMS Series). I would like to know if it is possible to install RMS lens to DCC1645C and get the microscopic image. If the answer is not, how we can integrate such kind of imaging systm by using your existing products. Look forward to getting your advice ASAP. Albert Lin Unique Instruments Co., Ltd. 6F., No.15, Lane 12, Xingzhong Rd., Nangang District, Taipei City 115, Taiwan (R.O.C.) Web: www.unii.com.tw email: albert@unii.com.tw Tel:+886-2-2654-6101 Fax:+886-2-2654-6102
Poster: Adam
Posted Date: 2010-03-24 11:35:49.0
A response from Adam at Thorlabs: At this time, Olympus does not provide us with information on the focal shift of these lenses. Since we cannot get this information from Olympus, we cannot provide concrete numbers. The RMS40X is designed more for red and blue wavelengths, so the shift from 650-750nm will be slightly larger than that of a typical objective, 3-4um. The focal shift of the C340TME-B lens is 20um over the range of 650-750nm. I noticed that an email address was not provided. If you have further questions, please feel free to respond to the feedback or send an email to techsupport@thorlabs.com.
Poster:
Posted Date: 2010-03-23 11:23:28.0
I am interested in the typical focal shift around 650-750nm. In addition I would like to know the maximum diffraction limited range around 700nm. I am thinking about this objective or the C340TME-B lens, to collect light from a point like ligth source in the above mentionned wavelength regime. Thanks
Poster: klee
Posted Date: 2009-10-19 18:26:23.0
A response from Ken at Thorlabs to plouis: These objectives are primarily designed for blue and red region. However, they still have about 83% transmittance at 800nm so they should work.
Poster: plouis
Posted Date: 2009-10-19 16:45:10.0
I would like to know if it possible to use these lenses for coupling 830 nm light (photons from SPDC) into SM (and MM) fibers using your fiber launch system.
Poster: technicalmarketing
Posted Date: 2009-03-22 08:33:38.0
Response to jbao from Inge at Thorlabs: These microscope objectives can be mounted to our lens tubes using an SM1A3 adapter.
Poster: jbao
Posted Date: 2009-03-21 17:07:00.0
do you have tube lens for these objectives?
Poster: technicalmarketing
Posted Date: 2008-02-07 09:14:45.0
In response to dutertes inquiry, we have contacted our optics department. Unfortunately, Olympus does not provide us with transmission curves for their objectives. I apologize that we cannot be of more help to you. Perhaps Olympus would be willing to provide that information if you contacted then directly.
Poster: duterte
Posted Date: 2008-02-05 04:40:14.0
Could you please tell me what are the transmissions for the infinity-corrected microscope objectives UIS2 : - 4X Microscope Objective, 18.5 mm WD - 10X Microscope Objective, 10.6 mm WD - 20X Microscope Objective, 0.4 NA, 1.2 mm WD - 40X Microscope Objective, 0.65 NA, 0.6 mm WD At 1064 nm and if it’s possible at 1550 nm. With best regards, Charles DUTERTE
Poster: technicalmarketing
Posted Date: 2007-10-25 15:12:01.0
We are currently in the process of updating this page to inlcude new items. A link to microscopy tools will appear in the family image shortly.
Poster: acable
Posted Date: 2007-10-25 14:32:36.0
It would be nice to link the Microscopy Tools section to this product page.
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Olympus Plan Achromat Objectives
  Plan Achromat Design for Air Applications
  Infinity Corrected Design
  Ideal for Imaging or Focusing Laser Light
  RMS (0.800"-36) Threading

These infinity-corrected, imaging, visible microscope objectives provide 4X, 10X, 20X, or 40X magnification. With their high numerical apertures (NA) and large magnifications, they are suitable for focusing or collimating laser light. These Olympus objectives are ideal for imaging applications due to their diffraction-limited performance across the entire visible spectrum. Alternatively, they can be used to focus light to a diffraction-limited spot, thus enabling efficient coupling of monochromatic or broadband light into a waveguide or fiber.

Their designation as Plan Achromats indicates that they are flat field and aberration corrected at two different wavelengths in the visible spectrum, leading to better spherical and chromatic corrections and superb field flatness. These achromatic objectives have an ultra wide antireflection coating and standard RMS threading.

Based on your currency / country selection, your order will ship from Newton, New Jersey  
+1 Qty Docs Part Number - Universal/Imperial Price Available / Ships
RMS4X Support Documentation RMS4X 4X Olympus Plan Achromat Objective, 0.10 NA, 18.5 mm WD $196.00
Today
RMS10X Support Documentation RMS10X 10X Olympus Plan Achromat Objective, 0.25 NA, 10.6 mm WD $388.00
Today
RMS20X Support Documentation RMS20X 20X Olympus Plan Achromat Objective, 0.4 NA, 1.2 mm WD $472.00
Today
RMS40X Support Documentation RMS40X 40X Olympus Plan Achromat Objective, 0.65 NA, 0.6 mm WD $784.00
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Olympus Plan Fluorite Objectives
  • Plan Fluorite Design
  • Infinity-Corrected Design
  • Ideal for Imaging or Focusing Laser Light
  • RMS (0.800"-36) Threading

These infinity-corrected, imaging, visible microscope objectives provide 4X, 10X, 20X, 40X, and 60X magnification. Plan Fluorite objectives, also called a plan semi-apochromat, are corrected for four wavelengths. These are well suited for color photomicrography. With high signal-to-noise ratios, excellent resolution, and high contrast imaging, they are useful in brightfield and Nomarski DIC observations.

The correction collar on the RMS60X-PFC rotates. By rotating the collar, the distance between the objective elements is changed, thereby correcting for the cover glass thickness.

Based on your currency / country selection, your order will ship from Newton, New Jersey  
+1 Qty Docs Part Number - Universal/Imperial Price Available / Ships
RMS4X-PF Support Documentation RMS4X-PF 4X Olympus Plan Fluorite Objective, 0.13 NA, 17 mm WD $547.00
Today
RMS10X-PF Support Documentation RMS10X-PF 10X Olympus Plan Fluorite Objective, 0.3 NA, 10 mm WD $979.00
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RMS20X-PF Support Documentation RMS20X-PF 20X Olympus Plan Fluorite Objective, 0.5 NA, 2.1 mm WD $1,095.00
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RMS40X-PF Support Documentation RMS40X-PF 40X Olympus Plan Fluorite Objective, 0.75 NA, 0.51 mm WD $1,272.00
Today
RMS60X-PFC Support Documentation RMS60X-PFC 60X Olympus Plan Fluorite Objective with Correction Collar, 0.9 NA, 0.2 mm WD $3,228.00
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Nikon Plan Fluorite Objectives
  Plan Fluorite Design
  Infinity-Corrected Design
  Ideal for Imaging or Focusing Laser Light
  M25 x 0.75 Threading

The Nikon Plan Fluorite Objectives provide 4X, 10X, 20X, 40X, 60X or 100X magnification. They are designed to have a high transmission rate (particularly in the UV wavelength) and to produce flat images across the field of view. Thus, these objectives are well suited for use in color laser scanning microscopy. These multi-purpose objectives can be utilized for brightfield, fluorescence, polarizing, and DIC observations. Of all the objectives featured on this page, thes Nikon plan fluor objectives are the most compatible with the Infinity-Corrected Tube Lens. They can then be incorporated into complex optical systems by using the SM2A20 adapter and Thorlabs' standard SM2 lens tube components.

Based on your currency / country selection, your order will ship from Newton, New Jersey  
+1 Qty Docs Part Number - Universal/Imperial Price Available / Ships
N4X-PF Support Documentation N4X-PF 4X Nikon Plan Fluorite Imaging Objective, 0.13 NA, 17.2 mm WD $432.00
3-5 Days
N10X-PF Support Documentation N10X-PF 10X Nikon Plan Fluorite Imaging Objective, 0.3 NA, 16 mm WD $785.00
3-5 Days
N20X-PF Support Documentation N20X-PF 20X Nikon Plan Fluorite Imaging Objective, 0.50 NA, 2.1 mm WD $870.00
3-5 Days
N40X-PF Support Documentation N40X-PF 40X Nikon Plan Fluorite Imaging Objective, 0.75 NA, 0.66 mm WD $991.00
3-5 Days
N60X-PF Support Documentation N60X-PF 60X Nikon Plan Fluorite microscope objective, 0.85 NA, 0.31 - 0.4 mm WD $2,334.00
3-5 Days
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Olympus Oil Immersion Objectives
  • Oil Immersion Objectives for the Visible Portion of the Spectrum
  • Plan Fluorite Design or Plan Achromat Design
  • Iris Diaphragm on RMS60X-PFOD and RMS100X-PFOD for Darkfield Microscopy
  • Infinity-Corrected Design
  • RMS (0.800"-36) Threading

These objectives provide 40X, 60X, or 100X magnification. They are designed for use in oil immersion applications; for more details on the benefits and applications of oil immersion and air objectives, please see the Objective Tutorial tab above. These objectives provide flat images across the entire visible spectrum. With their high signal-to-noise ratio, excellent resolution, and high-contrast imaging capabilities; they are effective in brightfield and Nomarski DIC observations. The RMS100X-PFO objective is also suitable for Optical Tweezer applications due to its nigh NA.

The majority of the objectives are plan fluorite objectives, while one (RMS100X-O) is a plan achromat design; the differences between these designs can be found in the Objective Tutorial tab. The RMS60X-PFOD and the RMS100X-PFOD objectives feature a built-in iris diaphragm, which is designed to be partially closed during darkfield microscopy, preserving the darkness of the background in darkfield observation. This is absolutely necessary for high numerical aperture (above NA = 1.2) oil immersion objectives when using an oil immersion darkfield condenser. For ordinary brightfield observations, the iris diaphragm should be left fully open. The iris diaphragm adjustment is pictured above and to the right.

The MOIL-30 Immersion Oil is also available below for use with these objectives.

Based on your currency / country selection, your order will ship from Newton, New Jersey  
+1 Qty Docs Part Number - Universal/Imperial Price Available / Ships
RMS40X-PFO Support Documentation RMS40X-PFO 40X Olympus Plan Fluorite Oil Immersion Objective, 1.3 NA, 0.2 mm WD $5,798.00
Today
RMS60X-PFOD Support Documentation RMS60X-PFOD 60X Olympus Plan Fluorite Oil Immersion Objective with Iris, 1.25-0.65 NA, 0.12 mm WD $3,375.00
Today
RMS100X-PFO Support Documentation RMS100X-PFO 100X Olympus Plan Fluorite Oil Immersion Objective, 1.30 NA, 0.20 mm WD $2,644.00
3-5 Days
RMS100X-PFOD Support Documentation RMS100X-PFOD 100X Olympus Plan Fluorite Oil Immersion Objective with Iris, 1.3-0.55 NA, 0.20 mm WD $3,184.00
Today
RMS100X-O Support Documentation RMS100X-O 100X Olympus Plan Achromat Oil Immersion Objective, 1.25 NA, 0.150mm WD $1,100.00
Today
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Nikon Oil Immersion Objective
  Plan Fluorite Design
  Designed for Oil Immersion Applications
  Ideal for High NA Imaging
  M25 x 0.75 Threading

This objective provides 100X magnification. It is designed for use in oil immersion applications; for more details on the benefits and applications of oil immersion and air objectives, please see the Objective Tutorial tab above.

This objective features a high transmission rate (particularly in the UV wavelength) and produces flat images across the field of view. Thus, this objective is well suited for use in color laser scanning microscopy. It can be utilized for brightfield, fluorescence, polarizing, and DIC observations. The high NA of this objective also makes it suitable for Optical Tweezer applications.

Based on your currency / country selection, your order will ship from Newton, New Jersey  
+1 Qty Docs Part Number - Universal/Imperial Price Available / Ships
N100X-PFO Support Documentation N100X-PFO 100X Nikon Plan Fluorite Oil immersion objective, 1.3 NA, 0.2 mm WD $2,112.00
3-5 Days
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Microscope Immersion Oil
  • Designed for use with Oil-Immersion Objectives
  • Low Auto-Fluorescence for Multiphoton Imaging

The MOIL-30 contains 30 mL of microscope immersion oil ideal for general microscopy. The low auto-fluorescence also makes it ideal for multiphoton and other fluorescence imaging microscopes.

Based on your currency / country selection, your order will ship from Newton, New Jersey  
+1 Qty Docs Part Number - Universal/Imperial Price Available / Ships
MOIL-30 Support Documentation MOIL-30 Low Auto Fluorescence Immersion Oil, 30 mL $45.00
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Microscope Objective Turret
  4 RMS-Threaded Objective Lens Ports
   SM1 (1.035"-40)-Threaded Mounting Interface
   Bi-Directional Repeatability: ±2.5 µm

The OT1 is a compact objective lens turret with four RMS-threaded ports. The housing is primarily constructed of aluminum but has steel inserts at critical points to ensure the durability and precision of the detent mechanism that reproducibly positions the objective lenses with a high degree of accuracy. The mounting interface for the OT1 objective lens turret has SM1 (1.035"-40) threading so that it can be easily integrated into custom imaging systems built using Thorlabs' Lens Tube and Cage Systems. To mount the OT1 in a cage system, use a CP02 or CP02T for a 30 mm cage system and an LCP02 for a 60 mm cage system.

Based on your currency / country selection, your order will ship from Newton, New Jersey  
+1 Qty Docs Part Number - Universal/Imperial Price Available / Ships
OT1 Support Documentation OT1 Objective Lens Turret, 4 RMS Threaded Objective Ports $285.00
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